Caracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrionii
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/7137 |
Resumo: | The polymorphic fungi Fonsecaea pedrosoi and Cladophialophora carrionii are the main etiological agents of Chromoblastomycosis (CBM), a chronic cutaneous mycotic infection, which occurs mainly in humid tropical regions. The scarcity of effective therapeutic protocols added to the clinical polymorphism of CBM leads to prolonged therapies and a high relapse rate. There are few data on the molecular biology of F. pedrosoi and C. carrionii, although some virulence factors have already been described, revealing the need to explore the mechanisms used by these pathogens during infection. Iron is a vital micronutrient for all organisms, with the exception of some bacteria, participating in essential cellular processes, such as: DNA replication, oxidative stress, cellular respiration, oxygen transport, energy production, among others. During the infection, the host decreases the availability of iron to the pathogen to contain the advancement of the infection; while the pathogen activates mechanisms for obtain and use the metal to survive in the infected tissues, revealing a scenario of intense dispute. In order to characterize the presence of these systems in F. pedrosoi and C. carrionii, in sílico analyzes were performed, revealing the presence of orthologs for proteins involved in reductive iron assimilation, siderophore mediated uptake and regulators of capture and utilization strategies of the metal, these sequences containing conserved domains consistent with the predicted functions. The expression profile of transcripts demonstrated the induction of iron reducing scavengers, siderophore biosynthesis intermediates and the hapX positive regulator during iron deprivation, in addition to repressing the negative regulatory sreA. The production of siderophores was observed after qualitative approaches, and the siderophore ferricrocine was subsequently identified intra- and extracellularly in F. pedrosoi and C. carrionii. These siderophores were able to restore the growth of a mutant strain of ΔsidA from A. nidulans. Preferred iron sources were observed after growth assays and, in addition, some of these sources were used by F. pedrosoi and C. carrionii during infection in macrophages, demonstrating that these pathogens may have strategies for remodeling iron homeostasis for survival. |
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Bailão, Alexandre Melohttp://lattes.cnpq.br/5415221996976886Bailão, Mirelle Garcia Silvahttp://lattes.cnpq.br/8844829751376156Bailão, Alexandre Melohttp://lattes.cnpq.br/5415221996976886Soares, Célia Maria de AlmeidaSchrank, Augustohttp://lattes.cnpq.br/3347135768456734Silva, Kassyo Lobato Potenciano da2017-04-13T11:21:48Z2017-02-20SILVA, K. L. P. Caracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrionii. 2017. 99 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2017.http://repositorio.bc.ufg.br/tede/handle/tede/7137The polymorphic fungi Fonsecaea pedrosoi and Cladophialophora carrionii are the main etiological agents of Chromoblastomycosis (CBM), a chronic cutaneous mycotic infection, which occurs mainly in humid tropical regions. The scarcity of effective therapeutic protocols added to the clinical polymorphism of CBM leads to prolonged therapies and a high relapse rate. There are few data on the molecular biology of F. pedrosoi and C. carrionii, although some virulence factors have already been described, revealing the need to explore the mechanisms used by these pathogens during infection. Iron is a vital micronutrient for all organisms, with the exception of some bacteria, participating in essential cellular processes, such as: DNA replication, oxidative stress, cellular respiration, oxygen transport, energy production, among others. During the infection, the host decreases the availability of iron to the pathogen to contain the advancement of the infection; while the pathogen activates mechanisms for obtain and use the metal to survive in the infected tissues, revealing a scenario of intense dispute. In order to characterize the presence of these systems in F. pedrosoi and C. carrionii, in sílico analyzes were performed, revealing the presence of orthologs for proteins involved in reductive iron assimilation, siderophore mediated uptake and regulators of capture and utilization strategies of the metal, these sequences containing conserved domains consistent with the predicted functions. The expression profile of transcripts demonstrated the induction of iron reducing scavengers, siderophore biosynthesis intermediates and the hapX positive regulator during iron deprivation, in addition to repressing the negative regulatory sreA. The production of siderophores was observed after qualitative approaches, and the siderophore ferricrocine was subsequently identified intra- and extracellularly in F. pedrosoi and C. carrionii. These siderophores were able to restore the growth of a mutant strain of ΔsidA from A. nidulans. Preferred iron sources were observed after growth assays and, in addition, some of these sources were used by F. pedrosoi and C. carrionii during infection in macrophages, demonstrating that these pathogens may have strategies for remodeling iron homeostasis for survival.Os fungos polimórficos Fonsecaea pedrosoi e Cladophialophora carrionii são os principais agentes etiológicos da Cromoblastomicose (CBM), uma infecção micótica cutânea crônica, que ocorre principalmente em regiões tropicais úmidas. A escassez de protocolos terapêuticos eficazes somada ao polimorfismo clínico da CBM, conduzem a terapias prolongadas e elevada taxa de recidiva. Poucos são os dados a respeito da biologia molecular de F. pedrosoi e C. carrionii, embora alguns fatores de virulência já tenham sido descritos, salientando a necessidade de se explorar os mecanismos utilizados por estes patógenos durante a infecção. O ferro é um micronutriente vital para todos os organismos, com exceção de algumas bactérias, participando de processos celulares essenciais, tais como: replicação do DNA, combate ao estresse oxidativo, respiração celular, transporte de oxigênio, produção de energia, dentre outros. Durante a infecção o hospedeiro diminui a disponibilidade de ferro para o agente patogênico para conter o avanço da infecção; enquanto o patógeno ativa mecanismos de obtenção e utilização do metal para sobreviver nos tecidos infectados, revelando um cenário de intensa disputa. Com o objetivo de caracterizar a presença destes sistemas em F. pedrosoi e C. carrionii, análises in sílico foram realizadas, revelando a presença de ortólogas para proteínas envolvidas na assimilação redutiva de ferro, captação mediada por sideróforos e reguladores das estratégias de captação e utilização do metal, contendo estas sequências domínios conservados consistentes com as funções preditas. O perfil de expressão de transcritos demonstrou a indução de captadores redutivos de ferro, intermediários da biossíntese de sideróforos e do regulador positivo hapX durante a privação de ferro, além da repressão do regulador negativo sreA. A produção de sideróforos foi observada após abordagens qualitativas, sendo posteriormente identificado o sideróforo ferricrocina intra e extracelularmente em F. pedrosoi e C. carrionii. Estes sideróforos mostraram-se capazes de restaurar o crescimento de uma linhagem mutante de ∆sidA de A. nidulans. Fontes preferenciais de ferro foram observadas após ensaios de crescimento e, além disso, algumas destas fontes foram utilizadas por F. pedrosoi e C. carrionii durante infecção em macrófagos, demonstrando que estes patógenos podem possuir estratégias de remodelamento da homeostase de ferro para sobrevivência.Submitted by JÚLIO HEBER SILVA (julioheber@yahoo.com.br) on 2017-04-12T17:27:16Z No. of bitstreams: 2 Dissertação - Kassyo Lobato Potenciano da Silva - 2017.pdf: 3631765 bytes, checksum: debcde89ec6bb553c1b37cafd56ee370 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-04-13T11:21:48Z (GMT) No. of bitstreams: 2 Dissertação - Kassyo Lobato Potenciano da Silva - 2017.pdf: 3631765 bytes, checksum: debcde89ec6bb553c1b37cafd56ee370 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2017-04-13T11:21:48Z (GMT). No. of bitstreams: 2 Dissertação - Kassyo Lobato Potenciano da Silva - 2017.pdf: 3631765 bytes, checksum: debcde89ec6bb553c1b37cafd56ee370 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-02-20Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Genética e Biologia MolecularUFGBrasilInstituto de Ciências Biológicas - ICB (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessVirulênciaSideróforosMetalVia redutivaReguladoresVirulenceSiderophoresMetalReductive pathwayRegulatorsGENETICA::GENETICA MOLECULAR E DE MICROORGANISMOSCaracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrioniiCaracterization of iron uptake mecanisms in Fonsecaea pedrosoi e Cladophialophora carrioniiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis7015780075895009588600600600600-3872772117827373404-7683082515614575372075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Caracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrionii |
dc.title.alternative.eng.fl_str_mv |
Caracterization of iron uptake mecanisms in Fonsecaea pedrosoi e Cladophialophora carrionii |
title |
Caracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrionii |
spellingShingle |
Caracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrionii Silva, Kassyo Lobato Potenciano da Virulência Sideróforos Metal Via redutiva Reguladores Virulence Siderophores Metal Reductive pathway Regulators GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS |
title_short |
Caracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrionii |
title_full |
Caracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrionii |
title_fullStr |
Caracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrionii |
title_full_unstemmed |
Caracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrionii |
title_sort |
Caracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrionii |
author |
Silva, Kassyo Lobato Potenciano da |
author_facet |
Silva, Kassyo Lobato Potenciano da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Bailão, Alexandre Melo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5415221996976886 |
dc.contributor.advisor-co1.fl_str_mv |
Bailão, Mirelle Garcia Silva |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/8844829751376156 |
dc.contributor.referee1.fl_str_mv |
Bailão, Alexandre Melo |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/5415221996976886 |
dc.contributor.referee2.fl_str_mv |
Soares, Célia Maria de Almeida |
dc.contributor.referee3.fl_str_mv |
Schrank, Augusto |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3347135768456734 |
dc.contributor.author.fl_str_mv |
Silva, Kassyo Lobato Potenciano da |
contributor_str_mv |
Bailão, Alexandre Melo Bailão, Mirelle Garcia Silva Bailão, Alexandre Melo Soares, Célia Maria de Almeida Schrank, Augusto |
dc.subject.por.fl_str_mv |
Virulência Sideróforos Metal Via redutiva Reguladores |
topic |
Virulência Sideróforos Metal Via redutiva Reguladores Virulence Siderophores Metal Reductive pathway Regulators GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS |
dc.subject.eng.fl_str_mv |
Virulence Siderophores Metal Reductive pathway Regulators |
dc.subject.cnpq.fl_str_mv |
GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS |
description |
The polymorphic fungi Fonsecaea pedrosoi and Cladophialophora carrionii are the main etiological agents of Chromoblastomycosis (CBM), a chronic cutaneous mycotic infection, which occurs mainly in humid tropical regions. The scarcity of effective therapeutic protocols added to the clinical polymorphism of CBM leads to prolonged therapies and a high relapse rate. There are few data on the molecular biology of F. pedrosoi and C. carrionii, although some virulence factors have already been described, revealing the need to explore the mechanisms used by these pathogens during infection. Iron is a vital micronutrient for all organisms, with the exception of some bacteria, participating in essential cellular processes, such as: DNA replication, oxidative stress, cellular respiration, oxygen transport, energy production, among others. During the infection, the host decreases the availability of iron to the pathogen to contain the advancement of the infection; while the pathogen activates mechanisms for obtain and use the metal to survive in the infected tissues, revealing a scenario of intense dispute. In order to characterize the presence of these systems in F. pedrosoi and C. carrionii, in sílico analyzes were performed, revealing the presence of orthologs for proteins involved in reductive iron assimilation, siderophore mediated uptake and regulators of capture and utilization strategies of the metal, these sequences containing conserved domains consistent with the predicted functions. The expression profile of transcripts demonstrated the induction of iron reducing scavengers, siderophore biosynthesis intermediates and the hapX positive regulator during iron deprivation, in addition to repressing the negative regulatory sreA. The production of siderophores was observed after qualitative approaches, and the siderophore ferricrocine was subsequently identified intra- and extracellularly in F. pedrosoi and C. carrionii. These siderophores were able to restore the growth of a mutant strain of ΔsidA from A. nidulans. Preferred iron sources were observed after growth assays and, in addition, some of these sources were used by F. pedrosoi and C. carrionii during infection in macrophages, demonstrating that these pathogens may have strategies for remodeling iron homeostasis for survival. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-04-13T11:21:48Z |
dc.date.issued.fl_str_mv |
2017-02-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, K. L. P. Caracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrionii. 2017. 99 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2017. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/7137 |
identifier_str_mv |
SILVA, K. L. P. Caracterização dos mecanismos de captação de ferro em Fonsecaea pedrosoi e Cladophialophora carrionii. 2017. 99 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2017. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/7137 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
7015780075895009588 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-3872772117827373404 |
dc.relation.cnpq.fl_str_mv |
-768308251561457537 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Genética e Biologia Molecular |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Ciências Biológicas - ICB (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/5b3b3e16-8f8d-4ee7-a442-a4ed7519a12d/download http://repositorio.bc.ufg.br/tede/bitstreams/99c80eac-8e16-4418-b27a-96f70817e5e1/download http://repositorio.bc.ufg.br/tede/bitstreams/e206d84e-d07e-4f85-abe0-64d72ccb6876/download http://repositorio.bc.ufg.br/tede/bitstreams/5f85894f-1c83-4349-834e-6ac7d0a32c56/download http://repositorio.bc.ufg.br/tede/bitstreams/62cff4ca-f029-4d33-91b8-e3b801a19a37/download |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e debcde89ec6bb553c1b37cafd56ee370 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1798044393363472384 |