Desenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitro
Main Author: | |
---|---|
Publication Date: | 2017 |
Format: | Master thesis |
Language: | por |
Source: | Repositório Institucional da UFG |
Download full: | http://repositorio.bc.ufg.br/tede/handle/tede/8731 |
Summary: | The effects of ultraviolet radiation on a skin have been extensively studied. Chronic exposure to solar radiation has been considered to be the major cause of skin cancer and photoaging. As bioactive molecules derived from natural products have been researched for the development of sunscreens combining ability to absorb UV light with potential antioxidant activity. Sucupira oil (Pterodon spp.) is cited in the literature for its anti-inflammatory, anti-nociceptive and antioxidante activities, among others. This research aimed to develop an oil-based sucupira nanoemulsion using a method of high energy emulsification, and to evaluate the influence of components and parameters related to the method in the stability and size of the droplets of nanoemulsions. Also, to assess its safety and effectiveness for in vitro methods. The HLB value, the association of surfactants, the ratios between the aqueous / oil phases and surfactants, operating conditions, and number of cycles in the homogenizer will influence the stability, size and size distribution of the droplets exhibited by the nanoemulsions. The nanoemulsions show pH between 4.0 and 5.0, average diameter of the droplets around 150nm and polydispersity index lower than 0.2 (indicating narrow size distribution) after being submitted to two cycles in the high pressure homogenizer at a pressure of 500 bar. Formulations containing 10% oil, 3 or 4% surfactant and 86% water with HLB values between 11 and 13 were the nanoemulsions presenting stability at all temperatures examined for a 90-day study period. NE presented IC50 of 20 μg of oil/mL on a human keratinocyte cell line (HaCat). NE did not exhibit photoprotective activity by inhibition of COX-2 and nuclear factor NFκB. However, it showed a protective effect against oxidative stress and the reduction of proinflammatory cytokines (IL-6 and IL-8) induced by UVA. The results obtained suggest that sucupira oil nanoemulsion is a potentially candidate for photoprotection by interfering with events initiated after UV exposure at keratinocytes. |
id |
UFG-2_0f6551b533a0737d65f10eb573a126ab |
---|---|
oai_identifier_str |
oai:repositorio.bc.ufg.br:tede/8731 |
network_acronym_str |
UFG-2 |
network_name_str |
Repositório Institucional da UFG |
repository_id_str |
|
spelling |
Lima, Eliana Martinshttp://lattes.cnpq.br/7248774319455970Lima, Eliana MartinsPeres Filho, Marco JunioDiniz, Danielle Guimarães Almeidahttp://lattes.cnpq.br/1973449622882511Pacheco, Maiulle Teixeira2018-07-23T12:12:19Z2017-06-06PACHECO, M. T. Desenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitro. 2017. 96 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2017.http://repositorio.bc.ufg.br/tede/handle/tede/8731The effects of ultraviolet radiation on a skin have been extensively studied. Chronic exposure to solar radiation has been considered to be the major cause of skin cancer and photoaging. As bioactive molecules derived from natural products have been researched for the development of sunscreens combining ability to absorb UV light with potential antioxidant activity. Sucupira oil (Pterodon spp.) is cited in the literature for its anti-inflammatory, anti-nociceptive and antioxidante activities, among others. This research aimed to develop an oil-based sucupira nanoemulsion using a method of high energy emulsification, and to evaluate the influence of components and parameters related to the method in the stability and size of the droplets of nanoemulsions. Also, to assess its safety and effectiveness for in vitro methods. The HLB value, the association of surfactants, the ratios between the aqueous / oil phases and surfactants, operating conditions, and number of cycles in the homogenizer will influence the stability, size and size distribution of the droplets exhibited by the nanoemulsions. The nanoemulsions show pH between 4.0 and 5.0, average diameter of the droplets around 150nm and polydispersity index lower than 0.2 (indicating narrow size distribution) after being submitted to two cycles in the high pressure homogenizer at a pressure of 500 bar. Formulations containing 10% oil, 3 or 4% surfactant and 86% water with HLB values between 11 and 13 were the nanoemulsions presenting stability at all temperatures examined for a 90-day study period. NE presented IC50 of 20 μg of oil/mL on a human keratinocyte cell line (HaCat). NE did not exhibit photoprotective activity by inhibition of COX-2 and nuclear factor NFκB. However, it showed a protective effect against oxidative stress and the reduction of proinflammatory cytokines (IL-6 and IL-8) induced by UVA. The results obtained suggest that sucupira oil nanoemulsion is a potentially candidate for photoprotection by interfering with events initiated after UV exposure at keratinocytes.Os efeitos da radiação ultravioleta sobre a pele têm sido amplamente estudados. A exposição crônica à radiação solar tem sido considerada a maior causa de câncer de pele e fotoenvelhecimento. As moléculas bioativas oriundas de produtos naturais têm sido pesquisadas para o desenvolvimento de protetores solares aliando capacidade de absorção da luz UV com potencial atividade antioxidante. O óleo de sucupira (Pterodon spp.) é citado na literatura pela sua atividade anti-inflamatória, antinoceptiva, antioxidante, dentre outras. Esta pesquisa teve como objetivo desenvolver nanoemulsão à base de óleo de sucupira a partir de método que utiliza alta energia de emulsificação, bem como avaliar a influência dos componentes e dos parâmetros relacionados ao método de obtenção na estabilidade e tamanho dos glóbulos das nanoemulsões e posteriormente avaliar a sua segurança e possível atividade fotoprotetora in vitro. O valor de EHL, a associação de tensoativos, a proporção entre as fases aquosa/oleosa e tensoativo, e condições operacionais, com número de ciclos depassagem no homogeneizador influenciaram na estabilidade, tamanho e distribuição de tamanho das gotículas. As nanoemulsões apresentaram pH entre 4,0 e 5,0, diâmetro médio das gotículas em torno de 150nm e índice de polidispersão abaixo de 0,2 (indicando estreita distribuição de tamanho), após passagem por 2 ciclos a uma pressão de 500 bar em homogeneizador de alta pressão. As formulações contendo 10% de óleo, 3 ou 4% de tensoativo e 86% de água, com EHL variando de 11 a 13 foram as nanoemulsões que se mantiveram estáveis em todas as temperaturas analisadas pelo período de estudo de 90 dias. A formulação final apresentou IC50 de 20μg de óleo/mL sobre a linhagem de queratinócitos humanos HaCaT. A NE não apresentou atividade fotoprotetora por inibição da COX-2 e do fator nuclear NFκB. Entretanto, apresentou efeito protetor contra o estresse oxidativo e a redução de citocinas pró-inflamatórias (IL-6 e IL-8) induzidas por UVA. Os dados obtidos sugerem que a nanoemulsão contendo 10% de óleo de sucupira é um potencial candidato à fotoproteção ao interferir com os eventos iniciados após a exposição à radiação UV nos queratinócitos.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2018-07-23T11:59:43Z No. of bitstreams: 2 Dissertação - Maiulle Teixeira Pacheco - 2017.pdf: 5936031 bytes, checksum: 8e7de0b329d5ef575b26675f42aa8154 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-07-23T12:12:19Z (GMT) No. of bitstreams: 2 Dissertação - Maiulle Teixeira Pacheco - 2017.pdf: 5936031 bytes, checksum: 8e7de0b329d5ef575b26675f42aa8154 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-07-23T12:12:19Z (GMT). No. of bitstreams: 2 Dissertação - Maiulle Teixeira Pacheco - 2017.pdf: 5936031 bytes, checksum: 8e7de0b329d5ef575b26675f42aa8154 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-06-06Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciências Farmacêuticas (FF)UFGBrasilFaculdade Farmácia - FF (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessFotoenvelhecimentoÓleo de sucupiraEstresse oxidativoNanoemulsãoestabilidadePhotoagingSucupira oil nanoemulsionStabilityOxidative stressCIENCIAS DA SAUDE::FARMACIADesenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitroDevelopment and characterization of sucupira (Pterodon spp.) oil nanoemulsion, in vitro evaluation its safety and efficiencyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis824936988196152412600600600600601028116152420937569976364134497549962075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://repositorio.bc.ufg.br/tede/bitstreams/9d4060f4-f993-4db2-bb9f-91536404794f/downloadbd3efa91386c1718a7f26a329fdcb468MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.bc.ufg.br/tede/bitstreams/8699aa2f-968b-4042-bfde-08d0a0ae2999/download4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://repositorio.bc.ufg.br/tede/bitstreams/e293a42f-fd16-4e09-a0b0-ceadc3bf95f1/downloadd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://repositorio.bc.ufg.br/tede/bitstreams/9c3a2575-1ea3-4075-b968-4d5c56343953/downloadd41d8cd98f00b204e9800998ecf8427eMD54ORIGINALDissertação - Maiulle Teixeira Pacheco - 2017.pdfDissertação - Maiulle Teixeira Pacheco - 2017.pdfapplication/pdf5936031http://repositorio.bc.ufg.br/tede/bitstreams/4409b31c-90e5-4a93-ac5f-e1cd146e4c15/download8e7de0b329d5ef575b26675f42aa8154MD55tede/87312018-07-23 09:12:19.286http://creativecommons.org/licenses/by-nc-nd/4.0/Acesso Abertoopen.accessoai:repositorio.bc.ufg.br:tede/8731http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2018-07-23T12:12:19Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.eng.fl_str_mv |
Desenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitro |
dc.title.alternative.eng.fl_str_mv |
Development and characterization of sucupira (Pterodon spp.) oil nanoemulsion, in vitro evaluation its safety and efficiency |
title |
Desenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitro |
spellingShingle |
Desenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitro Pacheco, Maiulle Teixeira Fotoenvelhecimento Óleo de sucupira Estresse oxidativo Nanoemulsãoestabilidade Photoaging Sucupira oil nanoemulsion Stability Oxidative stress CIENCIAS DA SAUDE::FARMACIA |
title_short |
Desenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitro |
title_full |
Desenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitro |
title_fullStr |
Desenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitro |
title_full_unstemmed |
Desenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitro |
title_sort |
Desenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitro |
author |
Pacheco, Maiulle Teixeira |
author_facet |
Pacheco, Maiulle Teixeira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Lima, Eliana Martins |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7248774319455970 |
dc.contributor.referee1.fl_str_mv |
Lima, Eliana Martins |
dc.contributor.referee2.fl_str_mv |
Peres Filho, Marco Junio |
dc.contributor.referee3.fl_str_mv |
Diniz, Danielle Guimarães Almeida |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1973449622882511 |
dc.contributor.author.fl_str_mv |
Pacheco, Maiulle Teixeira |
contributor_str_mv |
Lima, Eliana Martins Lima, Eliana Martins Peres Filho, Marco Junio Diniz, Danielle Guimarães Almeida |
dc.subject.por.fl_str_mv |
Fotoenvelhecimento Óleo de sucupira Estresse oxidativo Nanoemulsãoestabilidade |
topic |
Fotoenvelhecimento Óleo de sucupira Estresse oxidativo Nanoemulsãoestabilidade Photoaging Sucupira oil nanoemulsion Stability Oxidative stress CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Photoaging Sucupira oil nanoemulsion Stability Oxidative stress |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::FARMACIA |
description |
The effects of ultraviolet radiation on a skin have been extensively studied. Chronic exposure to solar radiation has been considered to be the major cause of skin cancer and photoaging. As bioactive molecules derived from natural products have been researched for the development of sunscreens combining ability to absorb UV light with potential antioxidant activity. Sucupira oil (Pterodon spp.) is cited in the literature for its anti-inflammatory, anti-nociceptive and antioxidante activities, among others. This research aimed to develop an oil-based sucupira nanoemulsion using a method of high energy emulsification, and to evaluate the influence of components and parameters related to the method in the stability and size of the droplets of nanoemulsions. Also, to assess its safety and effectiveness for in vitro methods. The HLB value, the association of surfactants, the ratios between the aqueous / oil phases and surfactants, operating conditions, and number of cycles in the homogenizer will influence the stability, size and size distribution of the droplets exhibited by the nanoemulsions. The nanoemulsions show pH between 4.0 and 5.0, average diameter of the droplets around 150nm and polydispersity index lower than 0.2 (indicating narrow size distribution) after being submitted to two cycles in the high pressure homogenizer at a pressure of 500 bar. Formulations containing 10% oil, 3 or 4% surfactant and 86% water with HLB values between 11 and 13 were the nanoemulsions presenting stability at all temperatures examined for a 90-day study period. NE presented IC50 of 20 μg of oil/mL on a human keratinocyte cell line (HaCat). NE did not exhibit photoprotective activity by inhibition of COX-2 and nuclear factor NFκB. However, it showed a protective effect against oxidative stress and the reduction of proinflammatory cytokines (IL-6 and IL-8) induced by UVA. The results obtained suggest that sucupira oil nanoemulsion is a potentially candidate for photoprotection by interfering with events initiated after UV exposure at keratinocytes. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-06-06 |
dc.date.accessioned.fl_str_mv |
2018-07-23T12:12:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PACHECO, M. T. Desenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitro. 2017. 96 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2017. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/8731 |
identifier_str_mv |
PACHECO, M. T. Desenvolvimento e caracterização de nanoemulsão à base de óleo de sucupira (Pterodon spp.), avaliação de sua toxicidade e bioatividade in vitro. 2017. 96 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2017. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/8731 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
824936988196152412 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
6010281161524209375 |
dc.relation.cnpq.fl_str_mv |
6997636413449754996 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Ciências Farmacêuticas (FF) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Faculdade Farmácia - FF (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/9d4060f4-f993-4db2-bb9f-91536404794f/download http://repositorio.bc.ufg.br/tede/bitstreams/8699aa2f-968b-4042-bfde-08d0a0ae2999/download http://repositorio.bc.ufg.br/tede/bitstreams/e293a42f-fd16-4e09-a0b0-ceadc3bf95f1/download http://repositorio.bc.ufg.br/tede/bitstreams/9c3a2575-1ea3-4075-b968-4d5c56343953/download http://repositorio.bc.ufg.br/tede/bitstreams/4409b31c-90e5-4a93-ac5f-e1cd146e4c15/download |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e 8e7de0b329d5ef575b26675f42aa8154 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1798044344852152320 |