Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/7095 |
Resumo: | Oil palm (Elaeis guineensis) is the world’s major source of vegetable oil and it has been able to adapt to the Brazilian subtropical area. Its growth, largely done in Para, is affected by Fatal Yellowing (FY), a condition that can cause plant’s death and has unknown cause. The aim for this master’s project was to prospect and identify, using a metabolomics approach, possible biomarkers of FY. To accomplish this study, for the first time different transport and extraction methods were evaluated in order to verify the metabolic profile stability in this species. Significant differences were noted and described using a metabolic fingerprinting approach, with ultra high precision liquid chromatography – mass spectrometry (UHPLC-MS) as the chemical analysis and ANOVA simultaneous component analysis (ASCA) as the multivariate statistic tool. After this study, leaves with and without FY symptoms were transported using the previously optimizes protocol, analyzed and five potential biomarkers were identified and related to their metabolic pathways, using PCA as the chemometric tool. Finally, for the first time, the use of advanced analytical techniques as MALDI imaging, which has been highlighted as a powerful tool for identification of chemical compounds present on tissue surface with spatial localization, has been applied to oil palm leaves in order to perform a metabolic screening and search for the previously identified biomarkers. An analytical protocol was established for analysis with and without matrix application, for a wide range mass coverage and one of the possible biomarkers was detected using the matrix-free approach. |
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Abdelnur, Patrícia Verardihttp://lattes.cnpq.br/0386805742968055Abdelnur, Patrícia Verardihttp://lattes.cnpq.br/0386805742968055Vaz, Boniek GontijoChaves, Andréa Rodrigueshttp://lattes.cnpq.br/0552790058654932Rodrigues Neto, Jorge Candido2017-04-07T11:07:20Z2017-03-03RODRIGUES NETO, J. C. Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica. 2017. 75 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2017.http://repositorio.bc.ufg.br/tede/handle/tede/7095Oil palm (Elaeis guineensis) is the world’s major source of vegetable oil and it has been able to adapt to the Brazilian subtropical area. Its growth, largely done in Para, is affected by Fatal Yellowing (FY), a condition that can cause plant’s death and has unknown cause. The aim for this master’s project was to prospect and identify, using a metabolomics approach, possible biomarkers of FY. To accomplish this study, for the first time different transport and extraction methods were evaluated in order to verify the metabolic profile stability in this species. Significant differences were noted and described using a metabolic fingerprinting approach, with ultra high precision liquid chromatography – mass spectrometry (UHPLC-MS) as the chemical analysis and ANOVA simultaneous component analysis (ASCA) as the multivariate statistic tool. After this study, leaves with and without FY symptoms were transported using the previously optimizes protocol, analyzed and five potential biomarkers were identified and related to their metabolic pathways, using PCA as the chemometric tool. Finally, for the first time, the use of advanced analytical techniques as MALDI imaging, which has been highlighted as a powerful tool for identification of chemical compounds present on tissue surface with spatial localization, has been applied to oil palm leaves in order to perform a metabolic screening and search for the previously identified biomarkers. An analytical protocol was established for analysis with and without matrix application, for a wide range mass coverage and one of the possible biomarkers was detected using the matrix-free approach.O dendê, fruto do dendezeiro (Elaeis guineensis) é a maior fonte de óleo vegetal do mundo e teve boa adaptação ao clima subtropical brasileiro. Seu cultivo no Brasil, que tem maior extensão no Pará, é afetado pelo Amarelecimento Fatal (AF), uma condição que pode provocar a morte da planta e tem causa desconhecida. O objetivo deste projeto de mestrado foi prospectar e identificar marcadores químicos relacionados ao AF utilizando uma abordagem metabolômica. Para que o estudo dessa causa fosse realizado, pela primeira vez foram avaliados diferentes métodos de transporte e extração de folhas para verificar a estabilidade do perfil metabólico desta espécie. Diferenças significativas foram observadas utilizando a estratégia de metabolic fingerprinting, com análise dos metabólitos por cromatografia de ultra alta eficiência – espectrometria de massas (UHPLC-MS) e análise quimiométrica dos dados por análise de componente simultâneo e análise de variância (ASCA). Após este estudo, folhas com e sem sintomas de AF foram transportadas utilizando protocolo otimizado anteriormente, analisadas e cinco potenciais biomarcadores foram identificados e relacionados as suas respectivas vias metabólicas, utilizando a análise de componentes principais (PCA) como ferramenta quimiométrica. Por fim, fez-se uso da recente e avançada técnica de imagem química em espectrometria de massas, com ionização por dessorção a laser assistida por matriz (MALDI-MS Imaging), que vem se destacando como uma poderosa ferramenta para a identificação de compostos químicos presentes na superfície de um tecido com localização espacial e foi utilizada pela primeira vez em um screening de metabólitos em folhas de dendê na busca dos marcadores previamente identificados. Um protocolo analítico foi desenvolvido para análise com aplicação de matriz e sem aplicação de matriz, com o intuito de abranger uma maior faixa de massas e um dos possíveis marcadores foi detectado utilizando a técnica de imagem sem matriz.Submitted by JÚLIO HEBER SILVA (julioheber@yahoo.com.br) on 2017-04-06T17:53:33Z No. of bitstreams: 2 Dissertação - Jorge Candido Rodrigues Neto - 2017.pdf: 4621226 bytes, checksum: 93409e3a28aced62d8c3f5dab02c58a7 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-04-07T11:07:20Z (GMT) No. of bitstreams: 2 Dissertação - Jorge Candido Rodrigues Neto - 2017.pdf: 4621226 bytes, checksum: 93409e3a28aced62d8c3f5dab02c58a7 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2017-04-07T11:07:20Z (GMT). No. of bitstreams: 2 Dissertação - Jorge Candido Rodrigues Neto - 2017.pdf: 4621226 bytes, checksum: 93409e3a28aced62d8c3f5dab02c58a7 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-03-03Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Química (IQ)UFGBrasilInstituto de Química - IQ (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessElaeis guineensisMetabolômicaEspectrometria de massasImagem químicaElaeis guineensisMetabolomicsMass spectrometryImagingQUIMICA ANALITICA::INSTRUMENTACAO ANALITICAProspecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômicaBiomarkers prospection related to fatal yellowing in Elaeis guineensis leaves using metabolomics approachinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis663693921325415158600600600600782606674374119727811432645100632034522075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALDissertação - Jorge Candido Rodrigues Neto - 2017.pdfDissertação - Jorge Candido Rodrigues Neto - 2017.pdfapplication/pdf4621226http://repositorio.bc.ufg.br/tede/bitstreams/315ac947-9b7b-435d-b355-00f92aaf38d7/download93409e3a28aced62d8c3f5dab02c58a7MD55LICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica |
dc.title.alternative.eng.fl_str_mv |
Biomarkers prospection related to fatal yellowing in Elaeis guineensis leaves using metabolomics approach |
title |
Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica |
spellingShingle |
Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica Rodrigues Neto, Jorge Candido Elaeis guineensis Metabolômica Espectrometria de massas Imagem química Elaeis guineensis Metabolomics Mass spectrometry Imaging QUIMICA ANALITICA::INSTRUMENTACAO ANALITICA |
title_short |
Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica |
title_full |
Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica |
title_fullStr |
Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica |
title_full_unstemmed |
Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica |
title_sort |
Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica |
author |
Rodrigues Neto, Jorge Candido |
author_facet |
Rodrigues Neto, Jorge Candido |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Abdelnur, Patrícia Verardi |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0386805742968055 |
dc.contributor.referee1.fl_str_mv |
Abdelnur, Patrícia Verardi |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/0386805742968055 |
dc.contributor.referee2.fl_str_mv |
Vaz, Boniek Gontijo |
dc.contributor.referee3.fl_str_mv |
Chaves, Andréa Rodrigues |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0552790058654932 |
dc.contributor.author.fl_str_mv |
Rodrigues Neto, Jorge Candido |
contributor_str_mv |
Abdelnur, Patrícia Verardi Abdelnur, Patrícia Verardi Vaz, Boniek Gontijo Chaves, Andréa Rodrigues |
dc.subject.por.fl_str_mv |
Elaeis guineensis Metabolômica Espectrometria de massas Imagem química |
topic |
Elaeis guineensis Metabolômica Espectrometria de massas Imagem química Elaeis guineensis Metabolomics Mass spectrometry Imaging QUIMICA ANALITICA::INSTRUMENTACAO ANALITICA |
dc.subject.eng.fl_str_mv |
Elaeis guineensis Metabolomics Mass spectrometry Imaging |
dc.subject.cnpq.fl_str_mv |
QUIMICA ANALITICA::INSTRUMENTACAO ANALITICA |
description |
Oil palm (Elaeis guineensis) is the world’s major source of vegetable oil and it has been able to adapt to the Brazilian subtropical area. Its growth, largely done in Para, is affected by Fatal Yellowing (FY), a condition that can cause plant’s death and has unknown cause. The aim for this master’s project was to prospect and identify, using a metabolomics approach, possible biomarkers of FY. To accomplish this study, for the first time different transport and extraction methods were evaluated in order to verify the metabolic profile stability in this species. Significant differences were noted and described using a metabolic fingerprinting approach, with ultra high precision liquid chromatography – mass spectrometry (UHPLC-MS) as the chemical analysis and ANOVA simultaneous component analysis (ASCA) as the multivariate statistic tool. After this study, leaves with and without FY symptoms were transported using the previously optimizes protocol, analyzed and five potential biomarkers were identified and related to their metabolic pathways, using PCA as the chemometric tool. Finally, for the first time, the use of advanced analytical techniques as MALDI imaging, which has been highlighted as a powerful tool for identification of chemical compounds present on tissue surface with spatial localization, has been applied to oil palm leaves in order to perform a metabolic screening and search for the previously identified biomarkers. An analytical protocol was established for analysis with and without matrix application, for a wide range mass coverage and one of the possible biomarkers was detected using the matrix-free approach. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-04-07T11:07:20Z |
dc.date.issued.fl_str_mv |
2017-03-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
RODRIGUES NETO, J. C. Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica. 2017. 75 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2017. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/7095 |
identifier_str_mv |
RODRIGUES NETO, J. C. Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica. 2017. 75 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2017. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/7095 |
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por |
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por |
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600 600 600 600 |
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7826066743741197278 |
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1143264510063203452 |
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2075167498588264571 |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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Universidade Federal de Goiás |
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Programa de Pós-graduação em Química (IQ) |
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UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Química - IQ (RG) |
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Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
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