Propriedades citotóxicas daβ lapachona em células de osteossarcoma caninoin vitro

• Autor(a) principal: Pimenta, Vanessa de Sousa Cruz
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/00130000062dz
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/5143
• topic: Cães; Cultivo celular; Métodos moleculares; Neoplasia óssea; Quinonas; Bone tumors; Cell culture; Dogs; Molecular methods; Quinones; CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL

Osteosarcoma is the most diagnosed primary bone cell tumor in dogs and humans. The need for more effective drugs with less intense collateral effect has triggered the development of plant-derived, natural-source chemotherapeutics. This study aimed to verify β lapachone intracellular effects on canine osteosarcoma cultured cells, as well as identify action mechanisms related to its antiproliferative properties. Cells were obtained from a cell line bank, sub cultivated and subjected to treatment with different β lapachone concentrations, followed by tetrazolium reduction, Tripan Blue dye exclusion assay, clongenic survival assay, Annexin V-FITC and propidium iodine double-labeling, JC-1 dye labeling and cell cycle kinetics analysis. The group treated with β lapachone for 72 hours showed the lowest cell viability, 27,74%, and the most conspicuous citotoxic effect, 64,81%, at 0,3 μM concentration; lower IC50, 0,180 μM and also the lowest cell growth - 0,50%- following treatment with 1,0 μM concentration. No statistical difference for cell proliferation was verified between concentrations after β lapachone exposure.Early apoptosis was the most frequent type of cell death considering all groups. It was less frequent in the 24-hour group treated with 0,1 μM (4,26 %) and more frequent in the 72-hour group treated with 1,0 μM (85,89 %). Mitochondrial depolarization was dose-dependent. Cell growth inhibition was carried out through cycle block at G0/G1 phase, according to exposure time. β lapachone was shown to have antiproliferative and cytotoxic effects, to induce apoptosis and to block cell cycle at G0/G1 phase on canine osteosarcoma cells.