Obtenção do plasma rico em plaquetas autólogo e sua ação sobre feridas cutâneas com autoenxertos em equinos

• Autor(a) principal: Pedroso, Ana Carolina Barros da Rosa
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/7890
• topic: Cavalos; Cicatrização; Concentração plaquetária; Enxertos de pele; Punch; Equine; Platelet concentration; Skin grafts; Wound healing; MEDICINA VETERINARIA::PATOLOGIA ANIMAL

The economic relevance of equine breeding in Brazil indicates the importance of the development of new economically viable treatments to promote tissue healing. Skin grafts and Platelet Rich Plasma (PRP) are available alternatives for improving the wound healing process in these animals. This study aimed to establish a simple method for autologous PRP production, as well as to evaluate its action on cutaneous wounds containing skin autografts in horses. Eight healthy horses were used, ranging in age from 3 to 18 years and mean live weight of 350 kg. For PRP obtention, a double centrifugation protocol was used. Two 6 x 6 cm wounds were performed on each side of the gluteal region. After the wound was filled by granulation tissue, punch autografts were obtained, with fragments harvested from the neck. Left-sided wounds were treated with PRP (GT) and right-sided wounds were not treated (GC). The protocol used to obtain PRP resulted in platelet concentration, on average, three times higher than that of whole blood. In the evaluation of PRP action in the healing of autografts, macroscopic and microscopic variables were considered. There was no significant difference between the groups (p> 0.05) for the variables inflammatory infiltrate, young fibroblasts, collagenization, epidermal thickness, macroscopic and microscopic integration of the autografts and retraction of the wound edges. However, neovascularization was significantly greater (p = 0.0191) in the PRP group, on the 14 th day after insertion of the autografts. It is concluded that the double centrifugation method results in PRP with adequate platelet concentration for therapeutic use. As for the action of PRP, this favors the process of skin repair with autografts in horses, since it increases the neovascularization in the initial phase of wound healing. Moreover, PRP seems to positively influence the integration of skin autografts and retraction of the wound edges, which is of fundamental importance for the reduction of hospitalization time and consequently of the therapeutic costs.