Estudo da toxicidade e genotoxicidade induzidas por diferentes nanopartículas in vivo
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/4353 |
Resumo: | The rapid advancement of nanotechnology has created a vast array of nanoparticles promising for industrial, energy and environmental sectors. Furthermore, many biological applications have been proposed for the nanoparticles, such as transport of genes and drugs for disease treatments, including cancer and infections. So, it is important to clarify if nanoparticles may represent a hazard to the environment and human health. In this study, we investigated the toxic potential and ability to induce DNA damage of different nanoparticles: four species of carbon nanotubes (NTC, NT, CS e MW), silver (AgNP) and nanoceria (CeO2-NP) nanoparticles. It was employed the somatic mutation and recombination test (SMART) in Drosophila melanogaster that detects the loss of heterozygosity of two genetic markers involved in the metabolic pathways of wing hairs formation - multiple wing hairs (mwh) and flare3 (flr3). In larvae, the proliferating of wings imaginal disc cells can produce hairs with mutant phenotypes expressed on the adult wings. Using the standard cross, third-stage larvae were treated with different concentrations of the nanoparticles until pupal stage. The wings of adult flies were examined microscopically for the identification of phenotypic abnormalities. In all concentrations the survival rates were higher than 90%, indicating the absence of chronic toxicity for nanoparticles evaluated. Using the conditional binomial test, the results of different treatments were compared with the respective negative control (distilled water), demonstrating no significantly increase in the NTC, NT and CS mutation and recombination frequencies (p>0.05) in mwh/flr3 genotype. In all carbon nanotubes tested, only the two higher concentrations of nanotubes MW (0.4 and 1 mg/mL) were able to induce genetic changes, mainly by mitotic recombination. The concentration of 10 mg/mL AgNP also promoted changes in the DNA and 61% of the phenotypic abnormalities were caused by recombination. The nanoceria was able to produce genotoxic effects at all concentrations tested (0.64-10 mg/mL). Overall, the mutational events were predominant, ranging from 46 to 72% of the total genotoxic effect induced by nanoceria, showing no dose-response relationship. In conclusion, our results demonstrated that carbon, silver and cerium dioxide nanoparticles have different genotoxic potential in D. melanogaster, so, another studies should be performed before any clinical and/or industrial application of nanoparticles. |
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Cunha, Kênya Silvahttp://lattes.cnpq.br/6124135410387685Cunha, Kênya SilvaLee, Chen ChenIrazusta, Silvia Pierrehttp://lattes.cnpq.br/9709334860647035Andrade, Laise Rodrigues de2015-03-26T13:07:25Z2012-02-27ANDRADE, L. R. Estudo da toxicidade e genotoxicidade induzidas por diferentes nanopartículas in vivo. 2012. 83 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2012.http://repositorio.bc.ufg.br/tede/handle/tede/4353ark:/38995/00130000032d9The rapid advancement of nanotechnology has created a vast array of nanoparticles promising for industrial, energy and environmental sectors. Furthermore, many biological applications have been proposed for the nanoparticles, such as transport of genes and drugs for disease treatments, including cancer and infections. So, it is important to clarify if nanoparticles may represent a hazard to the environment and human health. In this study, we investigated the toxic potential and ability to induce DNA damage of different nanoparticles: four species of carbon nanotubes (NTC, NT, CS e MW), silver (AgNP) and nanoceria (CeO2-NP) nanoparticles. It was employed the somatic mutation and recombination test (SMART) in Drosophila melanogaster that detects the loss of heterozygosity of two genetic markers involved in the metabolic pathways of wing hairs formation - multiple wing hairs (mwh) and flare3 (flr3). In larvae, the proliferating of wings imaginal disc cells can produce hairs with mutant phenotypes expressed on the adult wings. Using the standard cross, third-stage larvae were treated with different concentrations of the nanoparticles until pupal stage. The wings of adult flies were examined microscopically for the identification of phenotypic abnormalities. In all concentrations the survival rates were higher than 90%, indicating the absence of chronic toxicity for nanoparticles evaluated. Using the conditional binomial test, the results of different treatments were compared with the respective negative control (distilled water), demonstrating no significantly increase in the NTC, NT and CS mutation and recombination frequencies (p>0.05) in mwh/flr3 genotype. In all carbon nanotubes tested, only the two higher concentrations of nanotubes MW (0.4 and 1 mg/mL) were able to induce genetic changes, mainly by mitotic recombination. The concentration of 10 mg/mL AgNP also promoted changes in the DNA and 61% of the phenotypic abnormalities were caused by recombination. The nanoceria was able to produce genotoxic effects at all concentrations tested (0.64-10 mg/mL). Overall, the mutational events were predominant, ranging from 46 to 72% of the total genotoxic effect induced by nanoceria, showing no dose-response relationship. In conclusion, our results demonstrated that carbon, silver and cerium dioxide nanoparticles have different genotoxic potential in D. melanogaster, so, another studies should be performed before any clinical and/or industrial application of nanoparticles.O rápido avanço da nanotecnologia permitiu a criação de uma grande variedade de nanopartículas promissoras para os setores industrial, energético e ambiental. Além disso, muitas aplicações biológicas têm sido propostas para as nanopartículas, como o transporte de genes e fármacos para o tratamento de doenças, incluindo o câncer e infecções. Portanto, é importante esclarecer se as nanopartículas podem representar um perigo ao ambiente e à saúde humana. Neste estudo, nós investigamos a toxicidade potencial e a capacidade de induzir danos ao DNA de diferentes nanopartículas: quatro espécies de nanotubos de carbono (NTC, NT, CS e MW), nanopartículas de prata (AgNP) e nanocéria (CeO2-NP). Foi utilizado o teste para detecção de mutação e recombinação somática (SMART) em Drosophila melanogaster que detecta a perda de heterozigose de dois marcadores genéticos envolvidos nas vias metabólicas de formação de pelos da asa – pelos múltiplos (mwh) e flare3 (flr3). Nas larvas, a proliferação das células dos discos imaginais das asas pode produzir pelos com fenótipos mutantes expressos nas asas dos indivíduos adultos. Usando o cruzamento padrão, larvas de terceiro estágio foram tratadas com diferentes concentrações das nanopartículas até atingir o estágio de pupa. As asas das moscas adultas foram examinadas microscopicamente para identificação de alterações fenotípicas. Em todas as concentrações as taxas de sobrevivência foram superiores a 90%, indicando ausência de toxicidade crônica das nanopartículas avaliadas. Usando o teste binomial condicional, os resultados dos diferentes tratamentos com NTC, NT e CS foram comparados com o respectivo controle negativo (água destilada), demonstrando que não houve aumento estatisticamente significativo (p>0,05) nas frequências de mutação e recombinação no genótipo mwh/flr3. De todos os nanotubos de carbono avaliados, apenas as duas maiores concentrações do nanotubo MW (0,4 e 1 mg/mL) foram capazes de induzir alterações genéticas, principalmente via recombinação mitótica. A concentração de 10 mg/mL de AgNP também promoveu alterações no DNA e 61% das anormalidades fenotípicas foram causadas por recombinação. A nanocéria foi capaz de produzir efeitos genotóxicos em todas as concentrações testadas (0,64-10 mg/mL). Destes efeitos, os eventos mutacionais foram predominantes, variando de 46 a 72% do efeito genotóxico total induzido pela nanocéria, sem demonstrar efeito dose resposta. Em conclusão, nossos resultados demonstraram que nanopartículas constituídas de carbono, prata e dióxido de cério têm potenciais genotóxicos distintos em D. melanogaster, portanto, outros estudos devem ser realizados antes de qualquer aplicação clínica e/ou industrial das nanopartículas.Submitted by Luanna Matias (lua_matias@yahoo.com.br) on 2015-03-25T20:56:40Z No. of bitstreams: 2 Dissertação - Laise Rodrigues de Andrade - 2012.pdf: 1986605 bytes, checksum: 71d7e662c6200f1a50a70ae9addb99b0 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luanna Matias (lua_matias@yahoo.com.br) on 2015-03-26T13:07:25Z (GMT) No. of bitstreams: 2 Dissertação - Laise Rodrigues de Andrade - 2012.pdf: 1986605 bytes, checksum: 71d7e662c6200f1a50a70ae9addb99b0 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2015-03-26T13:07:25Z (GMT). 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dc.title.por.fl_str_mv |
Estudo da toxicidade e genotoxicidade induzidas por diferentes nanopartículas in vivo |
dc.title.alternative.por.fl_str_mv |
Study of taxicity and genotoxicity induced by different nenoparticles in vivo |
title |
Estudo da toxicidade e genotoxicidade induzidas por diferentes nanopartículas in vivo |
spellingShingle |
Estudo da toxicidade e genotoxicidade induzidas por diferentes nanopartículas in vivo Andrade, Laise Rodrigues de Genotoxicidade Nanopartículas Drosophila Genotoxicity Nanoparticles Drosophila GENETICA::MUTAGENESE |
title_short |
Estudo da toxicidade e genotoxicidade induzidas por diferentes nanopartículas in vivo |
title_full |
Estudo da toxicidade e genotoxicidade induzidas por diferentes nanopartículas in vivo |
title_fullStr |
Estudo da toxicidade e genotoxicidade induzidas por diferentes nanopartículas in vivo |
title_full_unstemmed |
Estudo da toxicidade e genotoxicidade induzidas por diferentes nanopartículas in vivo |
title_sort |
Estudo da toxicidade e genotoxicidade induzidas por diferentes nanopartículas in vivo |
author |
Andrade, Laise Rodrigues de |
author_facet |
Andrade, Laise Rodrigues de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Cunha, Kênya Silva |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6124135410387685 |
dc.contributor.referee1.fl_str_mv |
Cunha, Kênya Silva |
dc.contributor.referee2.fl_str_mv |
Lee, Chen Chen |
dc.contributor.referee3.fl_str_mv |
Irazusta, Silvia Pierre |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9709334860647035 |
dc.contributor.author.fl_str_mv |
Andrade, Laise Rodrigues de |
contributor_str_mv |
Cunha, Kênya Silva Cunha, Kênya Silva Lee, Chen Chen Irazusta, Silvia Pierre |
dc.subject.por.fl_str_mv |
Genotoxicidade Nanopartículas Drosophila |
topic |
Genotoxicidade Nanopartículas Drosophila Genotoxicity Nanoparticles Drosophila GENETICA::MUTAGENESE |
dc.subject.eng.fl_str_mv |
Genotoxicity Nanoparticles Drosophila |
dc.subject.cnpq.fl_str_mv |
GENETICA::MUTAGENESE |
description |
The rapid advancement of nanotechnology has created a vast array of nanoparticles promising for industrial, energy and environmental sectors. Furthermore, many biological applications have been proposed for the nanoparticles, such as transport of genes and drugs for disease treatments, including cancer and infections. So, it is important to clarify if nanoparticles may represent a hazard to the environment and human health. In this study, we investigated the toxic potential and ability to induce DNA damage of different nanoparticles: four species of carbon nanotubes (NTC, NT, CS e MW), silver (AgNP) and nanoceria (CeO2-NP) nanoparticles. It was employed the somatic mutation and recombination test (SMART) in Drosophila melanogaster that detects the loss of heterozygosity of two genetic markers involved in the metabolic pathways of wing hairs formation - multiple wing hairs (mwh) and flare3 (flr3). In larvae, the proliferating of wings imaginal disc cells can produce hairs with mutant phenotypes expressed on the adult wings. Using the standard cross, third-stage larvae were treated with different concentrations of the nanoparticles until pupal stage. The wings of adult flies were examined microscopically for the identification of phenotypic abnormalities. In all concentrations the survival rates were higher than 90%, indicating the absence of chronic toxicity for nanoparticles evaluated. Using the conditional binomial test, the results of different treatments were compared with the respective negative control (distilled water), demonstrating no significantly increase in the NTC, NT and CS mutation and recombination frequencies (p>0.05) in mwh/flr3 genotype. In all carbon nanotubes tested, only the two higher concentrations of nanotubes MW (0.4 and 1 mg/mL) were able to induce genetic changes, mainly by mitotic recombination. The concentration of 10 mg/mL AgNP also promoted changes in the DNA and 61% of the phenotypic abnormalities were caused by recombination. The nanoceria was able to produce genotoxic effects at all concentrations tested (0.64-10 mg/mL). Overall, the mutational events were predominant, ranging from 46 to 72% of the total genotoxic effect induced by nanoceria, showing no dose-response relationship. In conclusion, our results demonstrated that carbon, silver and cerium dioxide nanoparticles have different genotoxic potential in D. melanogaster, so, another studies should be performed before any clinical and/or industrial application of nanoparticles. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-27 |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:07:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ANDRADE, L. R. Estudo da toxicidade e genotoxicidade induzidas por diferentes nanopartículas in vivo. 2012. 83 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/4353 |
dc.identifier.dark.fl_str_mv |
ark:/38995/00130000032d9 |
identifier_str_mv |
ANDRADE, L. R. Estudo da toxicidade e genotoxicidade induzidas por diferentes nanopartículas in vivo. 2012. 83 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2012. ark:/38995/00130000032d9 |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/4353 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
6883982777473437920 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
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dc.relation.cnpq.fl_str_mv |
-4315821173933878065 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Biologia (ICB) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Ciências Biológicas - ICB (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
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Universidade Federal de Goiás (UFG) |
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UFG |
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UFG |
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Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/5e0f002a-75cd-4ff0-821b-2313c7b17c04/download http://repositorio.bc.ufg.br/tede/bitstreams/499bc169-bd0e-4bbb-b6dc-afd2ea243519/download http://repositorio.bc.ufg.br/tede/bitstreams/18e644ac-25f2-4c25-8e86-2cd4128a755d/download http://repositorio.bc.ufg.br/tede/bitstreams/2eb751d1-1835-4ba9-bc3c-d5ab5b7299ef/download http://repositorio.bc.ufg.br/tede/bitstreams/af35ad08-a66e-4ed0-a08e-fb41a458846a/download http://repositorio.bc.ufg.br/tede/bitstreams/ea9582c9-7cf1-4f98-b13d-2e4caff07978/download http://repositorio.bc.ufg.br/tede/bitstreams/0040d115-2305-4e25-903d-b06a3507d6e0/download |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f b292a83e42bd8ad62533bba1395b83ff 9da0b6dfac957114c6a7714714b86306 71d7e662c6200f1a50a70ae9addb99b0 8dc1a443107fe6d285950e4404ca585a b1fcef63339a5e551b85a29726b2f9f0 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1811721362019975168 |