Análise da tolerância á resistência em Trichoderma harzianum

• Autor(a) principal: Santos, Karina Roterdanny Araújo dos
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/00130000066xm
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/7940
• topic: Biocontrole; Resistência; Alumínio; Trichoderma harzianum; Biocontrol; Resistance; Aluminum; Trichoderma harzianum; CIENCIAS BIOLOGICAS::BIOQUIMICA

The presence of heavy metals in the soil has increased, causing many problems of an agricultural nature. Trichoderma are a species which is among the most well know and used biocontrol agents wordwide, as well as possessing qualities such tha parasitism and antibiosis, they have great resistance to metals and can also absorb them. Therefore, it is necessary for more detailed studies of the resistance of this fungus to metals and to research further the processes involved. In this work, we aimed to better understand the tolerance of T. harzianum to aluminum chloride, though a series of experiments performed in BDA, MEX e MYG, as well Bradford tests for quantification of proteins. In addition, we evaluated the gene response to metal stress through the sequencing RNA samples obtained when the fungus was created in BDA medium and submitted to concentrations of 1.5 and 3.0 mg / mL of aluminum chloride, 1.5 mg / mL, the fungus showed significant inhibition. It was observed that T. harzianum showed higher mycelial growth when grown in BDA medium compared to MEX and MYG, presenting levels of protein secretion inversely proportional to increasing concentrations of aluminum. The amount of transcriptionspecific factors of the stress response was increased, as well as the induction of genes involved in G-mediated cell signaling and increase in genes involved in vacuolar transport and carrier proteins. A repression of genes encoding proteins associated with cellular processes important for the growth of T. harzianum was also observed. These results show that T. harzianum is resistant to aluminum since it is able to tolerate and absorb large concentrations of this metal, which is commonly harmful to agriculture.