Estudo da morfogênese prostática em gerbilos normais e expostos a níveis ambientais de bisfenol-A durante o desenvolvimento intrauterino

• Autor(a) principal: Rodríguez, Daniel Andrés Osorio
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/0013000005gjx
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/9875
• topic: Seio urogenital; Próstata ventral; Próstata feminina; Bisfenol-A; Receptor de estrógenos alfa; Receptor de andrógenos; Antígeno nuclear de proliferação celular; Urogenital sinus; Ventral prostate; Female prostate; Bisphenol-A; Estrogen receptor alpha; Proliferating cell nuclear antigen; Androgen receptor; BIOQUIMICA::BIOLOGIA MOLECULAR

The prostate is an organ that during its development, growth, differentiation and homeostasis in adult life is under hormonal control, mainly by steroid hormones. Bisphenol-A (BPA) is a chemical used in the manufacture of polycarbonate-based products with the ability to mimic estrogens. The exposure to this compound during critical periods of development has caused permanent changes in several reproductive organs of females and males. The prostate is present in males and some species of female mammals. Little is known about prostate development in females and what are the mechanisms that govern it, further of its function in the female reproductive tract. The aim of this study was to describe comparatively the prostate development of male and female gerbils with one day of life and evaluate the effects of intrauterine exposure to environmental levels of BPA in the ventral prostate of males and the prostate of females of these rodents during the first day of neonatal life and a young-adult stage. For this, 20 female and 20 male of gerbils, all between 3 and 4 months old, were randomly matched for mating. We randomly matched one male to one female to form independent families. The mating day was determined by the presence of spermatozoids in the vaginal smears; this day was considered as day zero, being the initial day of the pregnancy period. Pregnant females received daily doses of mineral oil (control group) or 40 μg/kg/day of BPA (BPA group) from the 17th until the 24th day of gestation. After the birth, males and females were separated and euthanized with a day and ninety days. The prostates were collected for structural, cytochemical and immunohistochemical analysis. Furthermore, the prostate tissue of animals from one day of age was subjected to analysis three-dimensional reconstruction (3D). With this study, we describe the development of prostate compared gerbils males and females with a day of life, and evaluate the effects of exposure to BPA at levels similar to the spread in the environment, on the prostate of these animals. The results showed that there were no morphological alterations in the pattern of ductal budding and branching in the different experimental groups. In newborn males, there was a decrease in immunostaining for the androgen receptor (AR) and the proliferating cell nuclear antigen (PCNA). In adult males was observed a reduction in the relative frequency of the epithelial compartment. In newborn females was a decrease in AR and PCNA, and an increase in the immunostaining for estrogen receptor alpha (ERα). In adult females there was a significant increase in AR and PCNA. Thus, it can be concluded that BPA exposure during intrauterine life did not change the prostate development pattern or the morphology of the tissue in both, male and female newborns and adults. The effects of exposure are manifested differently in newborn and adult animals, due to changes in the hormonal environment that occur through the neonatal to adult life. Finally, the intrauterine exposure to BPA had more effect on the prostate gland of female gerbil.