Isolamento, seleção e cultivo de bactérias Produtoras de lipases para tratamento de Efluentes da indústria de alimentos

• Autor(a) principal: BUENO, Pabline Rafaella Mello
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tde/1435
• topic: hidrólise; tributirina; fermentação; hydrolysis; tributyrin; fermentation; CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA::TECNOLOGIA QUIMICA::ALIMENTOS

The present work was to isolate, select and cultivate microorganisms that produce lipases to apply the wastewater treatment industry of potato chips. For the isolation of microorganisms was used for industrial effluent and sewage treatment plant. The selection of the strain producing lipase was performed on solid medium containing tributyrin and in liquid medium, with p-nitrofenolpalmitato. Optimization of lipase production was performed as variables, the concentration of corn steep liquor, soybean oil and different mineral sources. The enzyme extract and the commercial enzyme selected strain were characterized on the optimal activity and stability at different temperatures and pH values and determined the molecular weight of the commercial enzyme. For the treatment of sewage, it has been characterized and has been the optimization of hydrolysis of triglycerides of the effluent having as variables, pH, enzyme concentration, emulsifier and the influence of fed batch and batch processes. Twenty-nine were isolated microorganisms were acquired and eleven research institutes. Of these, four microorganisms were pre-selected for their higher enzymatic activity. In the selection in submerged fermentation, enzymatic activity was the best strain of Burkholderia cepacia ATCC 25416. Optimization of lipase production was obtained with corn steep liquor (13.8 to 26.2 g.L-1) and sodium nitrate (1.5 to 3.9 g.L-1). In the characterization of the lipase enzyme extract and immobilized lipase from Burkholderia cepacia, the best results were found at 37ºC and pH 8.0. Were stable at 40°C, and thermosensitive at 50 and 60°C. The inactivation of lipases template denaturation followed first order. The molecular mass of commercial enzyme was 33 kDa. For the treatment of effluent, the maximum hydrolytic activity (0.310 U.mL-1) was observed when the volume of 7% gum arabic was set at 3 mL of pH between 7.47 to 7.92 and the concentration of enzyme 0.111 to 0.148 g.mL-1. The mathematical model obtained can be used for batch process and the predictive largest possible hydrolysis of the triglycerides present in the effluent.