Análise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassiceps
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
dARK ID: | ark:/38995/00130000027sh |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/4794 |
Resumo: | Experimental studies with Taenia crassiceps have been used to demonstrate its metabolic alterations in biochemical pathways (energetic and respiratory) in response to the presence of drugs. The use of T. crassiceps as an experimental model offers conditions to reproduce the neurocysticercosis infection one of the most severe form of human cysticercosis. This study aimed the analysis of the in vitro influence of niclosamide, an antihelminthic drug, on the metabolism of carbohydrates and fatty acids of T. crassiceps cysticerci (ORF strain). 20 larval stage cysticerci were cultured into 5mL of RPMI (Gibco) supplemented culture media, in 6 well culture plates, exposed or not to niclosamide (1, 2, and 3 uM). The control groups were performed with cysticerci not exposed to the drug, exposed to ethanol in the concentration used to dissolve the drug. The cysticerci were cultured at 37ºC for 24 hours. After this period the cysticerci were removed from the culture medium and both were frozen with liquid nitrogen. Afterward, the samples were processed for the HPLC analysis. Accordingly to the mode of action of this drug, which is to interfere in the electrons chain transport, the succinate concentrations were altered in the secretion/excretion of this parasite. Therefore, it is possible to conclude that in the concentrations used, this drug caused little alteration in the metabolic pathways of the parasite. |
id |
UFG-2_4077b07fc8e717c36effe25c585cb6bf |
---|---|
oai_identifier_str |
oai:repositorio.bc.ufg.br:tede/4794 |
network_acronym_str |
UFG-2 |
network_name_str |
Repositório Institucional da UFG |
repository_id_str |
|
spelling |
Vinaud, Marina Clarehttp://lattes.cnpq.br/1921551651088660Costa, Tatiane Luiza dahttp://lattes.cnpq.br/8219041935358486Vinaud, Marina ClareSilva, Luciana DamacenaBezerra, José Clecildo Barretohttp://lattes.cnpq.br/2742086994717950Costa, Marco Vítor Silva de Melo2015-10-28T10:10:53Z2015-03-09COSTA, Marco Vítor Silva de Melo. Análise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassiceps. 2015. 46 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/4794ark:/38995/00130000027shExperimental studies with Taenia crassiceps have been used to demonstrate its metabolic alterations in biochemical pathways (energetic and respiratory) in response to the presence of drugs. The use of T. crassiceps as an experimental model offers conditions to reproduce the neurocysticercosis infection one of the most severe form of human cysticercosis. This study aimed the analysis of the in vitro influence of niclosamide, an antihelminthic drug, on the metabolism of carbohydrates and fatty acids of T. crassiceps cysticerci (ORF strain). 20 larval stage cysticerci were cultured into 5mL of RPMI (Gibco) supplemented culture media, in 6 well culture plates, exposed or not to niclosamide (1, 2, and 3 uM). The control groups were performed with cysticerci not exposed to the drug, exposed to ethanol in the concentration used to dissolve the drug. The cysticerci were cultured at 37ºC for 24 hours. After this period the cysticerci were removed from the culture medium and both were frozen with liquid nitrogen. Afterward, the samples were processed for the HPLC analysis. Accordingly to the mode of action of this drug, which is to interfere in the electrons chain transport, the succinate concentrations were altered in the secretion/excretion of this parasite. Therefore, it is possible to conclude that in the concentrations used, this drug caused little alteration in the metabolic pathways of the parasite.Os estudos experimentais com o parasito Taenia crassiceps têm demonstrado alterações nas vias metabólicas (energética e respiratória) desse parasito, em resposta ao tratamento com fármacos. A utilização de Taenia crassiceps como modelo experimental oferece condições de se reproduzir a neurocisticercose, uma das formas mais graves da cisticercose humana. Este estudo tem como objetivo analisar a influência in vitro do fármaco anti-helmintico niclosamida, sobre o metabolismo de carboidratos e ácidos graxos de cisticercos de Taenia crassiceps (cepa ORF). Na realização deste estudo foram usados cisticercos em estágios larval de T. crassiceps (cepa ORF) e meio de cultura RPMI suplementado (Gibco). Foram utilizados 100 cisticercos, que foram alocados em placas de microcultura contendo seis poços, expostos ou não a niclosamida (1, 2 e 3 uM). Os grupos controle foram compostos por cisticercos não expostos ao fármaco e ao etanol na concentração utilizada para diluir o fármaco. Em seguida as placas foram à estufa a 37°C por 24 horas. Após esse período, os cisticercos foram retirados da cultura e ambos, parasito e meio de cultura, foram congelados em nitrogênio líquido. Na sequência os cisticercos foram processados para análise em HPLC. De acordo com o mecanismo de ação do fármaco, ou seja, interferência na cadeia transportadora de elétrons, nas concentrações utilizadas, observou-se uma alteração na secreção/ excreção do ácido orgânico succinato. As outras vias metabólicas não foram influenciadas pela ação deste fármaco. Portanto, conclui-se que a niclosamida apresenta baixa influencia no metabolismo dos cisticercos nas concentrações utilizadas.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2015-10-28T10:08:59Z No. of bitstreams: 2 Dissertação - Marco Vítor Silva de Melo Costa - 2015.pdf: 1166542 bytes, checksum: bcdd5fa5766049e7e29869d0734d92c8 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-10-28T10:10:53Z (GMT) No. of bitstreams: 2 Dissertação - Marco Vítor Silva de Melo Costa - 2015.pdf: 1166542 bytes, checksum: bcdd5fa5766049e7e29869d0734d92c8 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2015-10-28T10:10:53Z (GMT). No. of bitstreams: 2 Dissertação - Marco Vítor Silva de Melo Costa - 2015.pdf: 1166542 bytes, checksum: bcdd5fa5766049e7e29869d0734d92c8 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2015-03-09application/pdfhttp://repositorio.bc.ufg.br/tede/retrieve/22144/Disserta%c3%a7%c3%a3o%20-%20Marco%20V%c3%adtor%20Silva%20de%20Melo%20Costa%20-%202015.pdf.jpgporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia das Interações PH (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessÁcidos orgânicosTaenia crassicepsMetabolismoNiclosamidaOganic acidsTaenia crassicepsMetabolismNiclosamideCIENCIAS BIOLOGICAS::PARASITOLOGIAAnálise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassicepsBiochemical analysis of the niclosamida in metabolism of Taenia crassiceps cysticeriinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6367159433410056917600600600-7769011444564556288-4544576747271574306reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://repositorio.bc.ufg.br/tede/bitstreams/3cb1646e-8cb7-4a2c-b5c7-39cfefbd03cc/downloadbd3efa91386c1718a7f26a329fdcb468MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.bc.ufg.br/tede/bitstreams/522ca8a8-341f-4b77-8133-15c7d3bd35e1/download4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-821328http://repositorio.bc.ufg.br/tede/bitstreams/fcf365e8-88ad-4332-ba91-99548320a8c2/download683d9883b2ad62ac3b8bafc566b2e600MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-823148http://repositorio.bc.ufg.br/tede/bitstreams/30ef1620-9a7b-440c-bb3f-102a74d2ca7b/download9da0b6dfac957114c6a7714714b86306MD54ORIGINALDissertação - Marco Vítor Silva de Melo Costa - 2015.pdfDissertação - Marco Vítor Silva de Melo Costa - 2015.pdfapplication/pdf1166542http://repositorio.bc.ufg.br/tede/bitstreams/803eb856-645c-4503-9bf2-bf801fe9571b/downloadbcdd5fa5766049e7e29869d0734d92c8MD55TEXTDissertação - Marco Vítor Silva de Melo Costa - 2015.pdf.txtDissertação - Marco Vítor Silva de Melo Costa - 2015.pdf.txtExtracted Texttext/plain82041http://repositorio.bc.ufg.br/tede/bitstreams/612f3730-f337-4c7b-8f19-560c13367ee2/downloadf859f3767b92314e89b91da9ec163a21MD56THUMBNAILDissertação - Marco Vítor Silva de Melo Costa - 2015.pdf.jpgDissertação - Marco Vítor Silva de Melo Costa - 2015.pdf.jpgGenerated Thumbnailimage/jpeg2634http://repositorio.bc.ufg.br/tede/bitstreams/adcf3e14-356f-42d7-a033-68c1508a2c03/downloadf9ece41b4d692235d00dcf91357b8097MD57tede/47942015-10-29 03:03:45.248http://creativecommons.org/licenses/by-nc-nd/4.0/Acesso Abertoopen.accessoai:repositorio.bc.ufg.br:tede/4794http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2015-10-29T05:03:45Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)falseTk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSw5NQUklBIExJQ0VOw4dBCkVzdGEgbGljZW7Dp2EgZGUgZXhlbXBsbyDDqSBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTsOHQSBERSBESVNUUklCVUnDh8ODTyBOw4NPLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhw6fDo28gZGVzdGEgbGljZW7Dp2EsIHZvY8OqIChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlw7pkbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo= |
dc.title.por.fl_str_mv |
Análise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassiceps |
dc.title.alternative.eng.fl_str_mv |
Biochemical analysis of the niclosamida in metabolism of Taenia crassiceps cysticeri |
title |
Análise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassiceps |
spellingShingle |
Análise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassiceps Costa, Marco Vítor Silva de Melo Ácidos orgânicos Taenia crassiceps Metabolismo Niclosamida Oganic acids Taenia crassiceps Metabolism Niclosamide CIENCIAS BIOLOGICAS::PARASITOLOGIA |
title_short |
Análise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassiceps |
title_full |
Análise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassiceps |
title_fullStr |
Análise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassiceps |
title_full_unstemmed |
Análise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassiceps |
title_sort |
Análise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassiceps |
author |
Costa, Marco Vítor Silva de Melo |
author_facet |
Costa, Marco Vítor Silva de Melo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vinaud, Marina Clare |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1921551651088660 |
dc.contributor.advisor-co1.fl_str_mv |
Costa, Tatiane Luiza da |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/8219041935358486 |
dc.contributor.referee1.fl_str_mv |
Vinaud, Marina Clare |
dc.contributor.referee2.fl_str_mv |
Silva, Luciana Damacena |
dc.contributor.referee3.fl_str_mv |
Bezerra, José Clecildo Barreto |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2742086994717950 |
dc.contributor.author.fl_str_mv |
Costa, Marco Vítor Silva de Melo |
contributor_str_mv |
Vinaud, Marina Clare Costa, Tatiane Luiza da Vinaud, Marina Clare Silva, Luciana Damacena Bezerra, José Clecildo Barreto |
dc.subject.por.fl_str_mv |
Ácidos orgânicos Taenia crassiceps Metabolismo Niclosamida |
topic |
Ácidos orgânicos Taenia crassiceps Metabolismo Niclosamida Oganic acids Taenia crassiceps Metabolism Niclosamide CIENCIAS BIOLOGICAS::PARASITOLOGIA |
dc.subject.eng.fl_str_mv |
Oganic acids Taenia crassiceps Metabolism Niclosamide |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::PARASITOLOGIA |
description |
Experimental studies with Taenia crassiceps have been used to demonstrate its metabolic alterations in biochemical pathways (energetic and respiratory) in response to the presence of drugs. The use of T. crassiceps as an experimental model offers conditions to reproduce the neurocysticercosis infection one of the most severe form of human cysticercosis. This study aimed the analysis of the in vitro influence of niclosamide, an antihelminthic drug, on the metabolism of carbohydrates and fatty acids of T. crassiceps cysticerci (ORF strain). 20 larval stage cysticerci were cultured into 5mL of RPMI (Gibco) supplemented culture media, in 6 well culture plates, exposed or not to niclosamide (1, 2, and 3 uM). The control groups were performed with cysticerci not exposed to the drug, exposed to ethanol in the concentration used to dissolve the drug. The cysticerci were cultured at 37ºC for 24 hours. After this period the cysticerci were removed from the culture medium and both were frozen with liquid nitrogen. Afterward, the samples were processed for the HPLC analysis. Accordingly to the mode of action of this drug, which is to interfere in the electrons chain transport, the succinate concentrations were altered in the secretion/excretion of this parasite. Therefore, it is possible to conclude that in the concentrations used, this drug caused little alteration in the metabolic pathways of the parasite. |
publishDate |
2015 |
dc.date.accessioned.fl_str_mv |
2015-10-28T10:10:53Z |
dc.date.issued.fl_str_mv |
2015-03-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COSTA, Marco Vítor Silva de Melo. Análise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassiceps. 2015. 46 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/4794 |
dc.identifier.dark.fl_str_mv |
ark:/38995/00130000027sh |
identifier_str_mv |
COSTA, Marco Vítor Silva de Melo. Análise bioquímica da niclosamida no metabolismo de cisticercos de Taenia crassiceps. 2015. 46 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015. ark:/38995/00130000027sh |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/4794 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
6367159433410056917 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
-7769011444564556288 |
dc.relation.cnpq.fl_str_mv |
-4544576747271574306 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Biologia das Interações PH (IPTSP) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/3cb1646e-8cb7-4a2c-b5c7-39cfefbd03cc/download http://repositorio.bc.ufg.br/tede/bitstreams/522ca8a8-341f-4b77-8133-15c7d3bd35e1/download http://repositorio.bc.ufg.br/tede/bitstreams/fcf365e8-88ad-4332-ba91-99548320a8c2/download http://repositorio.bc.ufg.br/tede/bitstreams/30ef1620-9a7b-440c-bb3f-102a74d2ca7b/download http://repositorio.bc.ufg.br/tede/bitstreams/803eb856-645c-4503-9bf2-bf801fe9571b/download http://repositorio.bc.ufg.br/tede/bitstreams/612f3730-f337-4c7b-8f19-560c13367ee2/download http://repositorio.bc.ufg.br/tede/bitstreams/adcf3e14-356f-42d7-a033-68c1508a2c03/download |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f 683d9883b2ad62ac3b8bafc566b2e600 9da0b6dfac957114c6a7714714b86306 bcdd5fa5766049e7e29869d0734d92c8 f859f3767b92314e89b91da9ec163a21 f9ece41b4d692235d00dcf91357b8097 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1815172532609744896 |