Avaliação histoquímica e da expressão das proteínas p53 e c-KIT no mastocitoma canino

• Autor(a) principal: Pimenta, Vanessa de Sousa Cruz
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/0013000007db4
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/3576
• topic: Proto-oncogene; Sarcoma de mastócitos; Cães; Imunoistoquímica; Proteína supressora de tumor; Mast-cell sarcoma; Dogs; Immunohistochemistry; Tumor suppressor protein; Proto-oncogenes; MEDICINA VETERINARIA::PATOLOGIA ANIMAL

The objective of this study was to verify the pattern of staining by Bismarck Brown and evaluate the expression of p53 and c-KIT in neoplastic mast cells, quantifying the marking obtained by these antibodies using the Image J software and correlating the values found with histologic subtypes. Mast cell tumors are the most common skin neoplasms of dogs, is an excessive proliferation of neoplastic mast cells, of variable and unpredictable biological behavior, with great capacity of recurrences and metastasis. The lesions may be dermis or subcutaneous location and three grades are described. At the first, the tumor is well differentiated, moderately differentiated is the second and the third is little differentiated. Their cause is still unknown. One of the mechanisms proposed for the proliferation of mast cells is to change the nucleotide sequence of the c-KIT gene. The tumor suppressor gene TP53 is directly related to blockage of cell cycle and the protection is changed by mutations in the p53. Were reviewed 1242 protocols of histopathology exams obtained the archives of Laboratory of Animal Pathology/UFG, from January 2007 to April 2011, found 37 diagnosis of canine cutaneous mast cell tumor. Regarding the epidemiologic aspects the prevalence of 55.26% was noted in dogs in the age group 6-10 years, 63.16% female and 39.48% of the Boxer breed. The most common anatomic site was the hind-limb with 31.58% of records. Histologic evaluation and histochemistry using Hematoxylin - Eosin and Toluidine Blue allowed to classify the majority of mast cell tumors studied, although only 24.32% of the samples were identified using the color Bismarck Brown. The prevalence of Grade II was 43.24% of all cases The lowest value expressed for p53 and c-KIT, was in Grade II, the highest value in the Grade III and the highest average in grade I. Was concluded that the coloration of Bismarck Brown proved efficient to aid in the diagnostic accuracy of laboratory routine and utilization of antibodies anti p53 and anti c-kit promoted good immunostaining with important role to determine the prognosis of canine mast cell tumors.