Avaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivo
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/6424 |
Resumo: | Punicalagin and gemin D are ellagitannins found in some species of plants of medical importance such as Punica granatum and Geun japonicun. For this study, punicalagin and gemin D were isolated, respectively, from the leaves of Lafoensia pacari and Eugenia uniflora, two species of Brazilian medicinal plants with several biological activities, such as antitumoral, antioxidant and healing of wounds. In the present study, we evaluated the genotoxic, cytotoxic, antigenotoxic and anticytotoxic effects of gemin D using the Ames test in Salmonella typhimurium, the micronucleus (MN) test and comet assay in mice. With the punicalagin ellagitannin, we assessed the same effects mentioned above using the comet and MN tests in mice, and we also evaluated the angiogenic and antiangiogenic activities of this ellagitannin by the chick chorioallantoic membrane (CAM) angiogenic assay. The results obtained with gemin D showed that this tannin did not present genotoxic effect by the Ames and MN tests, however, in the comet assay, the highest dose of gemin D (100 mg/kg) induced increase of breaks in DNA in comparison to the negative control (p < 0.05). In the antigenotoxicity, gemin D protected DNA against the harmful action of 4-nitroquinoline-1-oxide and sodium azide by the Ames test, and also against cyclophosphamide (CPA) in pre- and co-treatment by MN and comet tests in mice, but it did not protect DNA in the post-treatment. The results obtained with punicalagin showed that this tannin exhibited no genotoxic effect by MN test and comet assay in mice. Only the highest dose of punicalagin (50 mg/kg) exhibited significant cytotoxic effect by MN test, and in the co-treatment with CPA, this cytotoxicity was enhanced. Co-treatment, pre-treatment and post-treatment of punicalagin with CPA led to a significant reduction in the number of DNA breaks and in the frequency of CPA-induced MN, indicating antigenotoxic effect. Using the CAM model, punicalagin exhibited angiogenic activity in all concentrations, mainly at the lowest concentration (12.5 µg/µL). Therefore, gemin D and punicalagin exhibited relevant antigenotoxic and cytotoxic effects, which indicate that they may be probables candidates for chemoprevention or for the development of new cancer therapies. In addition, the angiogenic activity presented by punicalagin in this study could contribute for the processes of tissue repairing and wound healing. |
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Lee, Chen Chenhttp://lattes.cnpq.br/4621907105842007Santos, Suzana da Costahttp://lattes.cnpq.br/7811945085200334Lee, Chen ChenReis, Paulo Roberto De MeloBailão, Elisa Flávia Luiz CardosoSapanó, Mário AntônioCruz, Aline Helena Da Silvahttp://lattes.cnpq.br/9930282180386562Carneiro, Cristiene Costa2016-10-19T16:08:06Z2016-09-16CARNEIRO, C.C. Avaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivo. 2016. 123 f. Tese (Doutorado em Biologia) -Universidade Federal de Goiás, Goiânia, 2016.http://repositorio.bc.ufg.br/tede/handle/tede/6424Punicalagin and gemin D are ellagitannins found in some species of plants of medical importance such as Punica granatum and Geun japonicun. For this study, punicalagin and gemin D were isolated, respectively, from the leaves of Lafoensia pacari and Eugenia uniflora, two species of Brazilian medicinal plants with several biological activities, such as antitumoral, antioxidant and healing of wounds. In the present study, we evaluated the genotoxic, cytotoxic, antigenotoxic and anticytotoxic effects of gemin D using the Ames test in Salmonella typhimurium, the micronucleus (MN) test and comet assay in mice. With the punicalagin ellagitannin, we assessed the same effects mentioned above using the comet and MN tests in mice, and we also evaluated the angiogenic and antiangiogenic activities of this ellagitannin by the chick chorioallantoic membrane (CAM) angiogenic assay. The results obtained with gemin D showed that this tannin did not present genotoxic effect by the Ames and MN tests, however, in the comet assay, the highest dose of gemin D (100 mg/kg) induced increase of breaks in DNA in comparison to the negative control (p < 0.05). In the antigenotoxicity, gemin D protected DNA against the harmful action of 4-nitroquinoline-1-oxide and sodium azide by the Ames test, and also against cyclophosphamide (CPA) in pre- and co-treatment by MN and comet tests in mice, but it did not protect DNA in the post-treatment. The results obtained with punicalagin showed that this tannin exhibited no genotoxic effect by MN test and comet assay in mice. Only the highest dose of punicalagin (50 mg/kg) exhibited significant cytotoxic effect by MN test, and in the co-treatment with CPA, this cytotoxicity was enhanced. Co-treatment, pre-treatment and post-treatment of punicalagin with CPA led to a significant reduction in the number of DNA breaks and in the frequency of CPA-induced MN, indicating antigenotoxic effect. Using the CAM model, punicalagin exhibited angiogenic activity in all concentrations, mainly at the lowest concentration (12.5 µg/µL). Therefore, gemin D and punicalagin exhibited relevant antigenotoxic and cytotoxic effects, which indicate that they may be probables candidates for chemoprevention or for the development of new cancer therapies. In addition, the angiogenic activity presented by punicalagin in this study could contribute for the processes of tissue repairing and wound healing.Punicalagin e gemin D são elagitaninos encontrados em algumas espécies de plantas de importância médica, tais como Punica granatum e Pelargonium sidoides. Para o presente estudo, punicalagin e gemin D foram isolados, respectivamente, das folhas de Lafoensia pacari e Eugenia uniflora, duas espécies de plantas medicinais brasileiras com diversas atividades biológicas, tais como, antitumoral, antioxidante e cicatrizante de feridas. No presente estudo, nós avaliamos os seguintes efeitos: genotóxico, citotóxico, antigenotóxico e anticitotóxico de gemin D utilizando o teste de Ames em Salmonella typhimurium, o teste do micronúcleo (MN) e o ensaio cometa em camundongos. Com o elagitanino punicalagin, nós investigamos os mesmos efeitos citados anteriormente utilizando os testes cometa e MN em camundongos, e também avaliamos a atividade angiogênica e antiangiogênica desse tanino utilizando o ensaio em membrana corioalantóide (MCA) do ovo embrionado de galinha. Os resultados obtidos com gemin D mostraram que esse tanino não apresentou efeito genotóxico pelos testes de Ames e MN, porém, no ensaio cometa, a maior dose de gemin D (100 mg/kg) induziu aumento de quebras no DNA em comparação com o controle negativo (p < 0.05). Na avaliação antigenotóxica, gemin D protegeu o DNA contra as ações lesivas de 4-nitroquinolina-1-óxido e azida sódica pelo teste de Ames, e também contra ciclofosfamida (CIF) no pré- e co-tratamento pelos testes cometa e MN em camundongos, entretanto, ele não protegeu o DNA no pós-tratamento. Os resultados obtidos com punicalagin mostraram que esse elagitanino não exibiu efeito genotóxico pelos testes cometa e MN em camundongos. Apenas a maior dose de punicalagin (50 mg/kg) exibiu efeito citotóxico significativo pelo teste do MN, e no co-tratamento com CIF, essa citotoxicidade foi maior do que a apresentada pelo controle positivo (CIF). O co-, pré e pós-tratamento de punicalagin com CIF em camundongos levou a uma redução significativa no número de quebras no DNA e na frequência de MN induzida por CIF, indicando efeito antigenotóxico. Utilizando o modelo MCA, punicalagin exibiu atividade angiogênica em todas as concentrações testadas, especialmente a menor concentração (12.5 µg/µL). Assim, gemin D e punicalagin demonstraram relevantes efeitos antigenotóxico e citotóxico, indicando que eles podem ser prováveis candidatos para quimioprevenção ou desenvolvimento de novas terapias para o câncer. Além disso, a atividade angiogênica apresentada por punicalagin nesse estudo poderia contribuir em processos de reparação tecidual e cicatrização de feridas, como por exemplo no tratamento de úlceras gástricas e queimaduras.Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2016-10-19T10:06:31Z No. of bitstreams: 2 Tese - Cristiene Costa Carneiro - 2016.pdf: 2763720 bytes, checksum: 5287cb8e3862369e99646711609afd00 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Jaqueline Silva (jtas29@gmail.com) on 2016-10-19T16:08:06Z (GMT) No. of bitstreams: 2 Tese - Cristiene Costa Carneiro - 2016.pdf: 2763720 bytes, checksum: 5287cb8e3862369e99646711609afd00 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2016-10-19T16:08:06Z (GMT). No. of bitstreams: 2 Tese - Cristiene Costa Carneiro - 2016.pdf: 2763720 bytes, checksum: 5287cb8e3862369e99646711609afd00 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-09-16Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessPunicalaginGemin DAntigenotoxicidadeQuimioprevençãoAngiogênesePunicalaginGemin DAntigenotoxicityChemopreventionAngiogenesisCIENCIAS BIOLOGICAS::GENETICACIENCIAS BIOLOGICAS::BIOQUIMICAAvaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivoAssessment of the activities genotoxic, antigenotoxic, cytotoxic, anticytotoxic, angiogenic and antiangiogenic of ellagitannins, using tests in vitro and in vivoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis6883982777473437920600600600600600-3872772117827373404-55181442685852520518934779903292661142075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Avaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivo |
dc.title.alternative.eng.fl_str_mv |
Assessment of the activities genotoxic, antigenotoxic, cytotoxic, anticytotoxic, angiogenic and antiangiogenic of ellagitannins, using tests in vitro and in vivo |
title |
Avaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivo |
spellingShingle |
Avaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivo Carneiro, Cristiene Costa Punicalagin Gemin D Antigenotoxicidade Quimioprevenção Angiogênese Punicalagin Gemin D Antigenotoxicity Chemoprevention Angiogenesis CIENCIAS BIOLOGICAS::GENETICA CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Avaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivo |
title_full |
Avaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivo |
title_fullStr |
Avaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivo |
title_full_unstemmed |
Avaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivo |
title_sort |
Avaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivo |
author |
Carneiro, Cristiene Costa |
author_facet |
Carneiro, Cristiene Costa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Lee, Chen Chen |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4621907105842007 |
dc.contributor.advisor-co1.fl_str_mv |
Santos, Suzana da Costa |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7811945085200334 |
dc.contributor.referee1.fl_str_mv |
Lee, Chen Chen |
dc.contributor.referee2.fl_str_mv |
Reis, Paulo Roberto De Melo |
dc.contributor.referee3.fl_str_mv |
Bailão, Elisa Flávia Luiz Cardoso |
dc.contributor.referee4.fl_str_mv |
Sapanó, Mário Antônio |
dc.contributor.referee5.fl_str_mv |
Cruz, Aline Helena Da Silva |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9930282180386562 |
dc.contributor.author.fl_str_mv |
Carneiro, Cristiene Costa |
contributor_str_mv |
Lee, Chen Chen Santos, Suzana da Costa Lee, Chen Chen Reis, Paulo Roberto De Melo Bailão, Elisa Flávia Luiz Cardoso Sapanó, Mário Antônio Cruz, Aline Helena Da Silva |
dc.subject.por.fl_str_mv |
Punicalagin Gemin D Antigenotoxicidade Quimioprevenção Angiogênese |
topic |
Punicalagin Gemin D Antigenotoxicidade Quimioprevenção Angiogênese Punicalagin Gemin D Antigenotoxicity Chemoprevention Angiogenesis CIENCIAS BIOLOGICAS::GENETICA CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Punicalagin Gemin D Antigenotoxicity Chemoprevention Angiogenesis |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::GENETICA CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Punicalagin and gemin D are ellagitannins found in some species of plants of medical importance such as Punica granatum and Geun japonicun. For this study, punicalagin and gemin D were isolated, respectively, from the leaves of Lafoensia pacari and Eugenia uniflora, two species of Brazilian medicinal plants with several biological activities, such as antitumoral, antioxidant and healing of wounds. In the present study, we evaluated the genotoxic, cytotoxic, antigenotoxic and anticytotoxic effects of gemin D using the Ames test in Salmonella typhimurium, the micronucleus (MN) test and comet assay in mice. With the punicalagin ellagitannin, we assessed the same effects mentioned above using the comet and MN tests in mice, and we also evaluated the angiogenic and antiangiogenic activities of this ellagitannin by the chick chorioallantoic membrane (CAM) angiogenic assay. The results obtained with gemin D showed that this tannin did not present genotoxic effect by the Ames and MN tests, however, in the comet assay, the highest dose of gemin D (100 mg/kg) induced increase of breaks in DNA in comparison to the negative control (p < 0.05). In the antigenotoxicity, gemin D protected DNA against the harmful action of 4-nitroquinoline-1-oxide and sodium azide by the Ames test, and also against cyclophosphamide (CPA) in pre- and co-treatment by MN and comet tests in mice, but it did not protect DNA in the post-treatment. The results obtained with punicalagin showed that this tannin exhibited no genotoxic effect by MN test and comet assay in mice. Only the highest dose of punicalagin (50 mg/kg) exhibited significant cytotoxic effect by MN test, and in the co-treatment with CPA, this cytotoxicity was enhanced. Co-treatment, pre-treatment and post-treatment of punicalagin with CPA led to a significant reduction in the number of DNA breaks and in the frequency of CPA-induced MN, indicating antigenotoxic effect. Using the CAM model, punicalagin exhibited angiogenic activity in all concentrations, mainly at the lowest concentration (12.5 µg/µL). Therefore, gemin D and punicalagin exhibited relevant antigenotoxic and cytotoxic effects, which indicate that they may be probables candidates for chemoprevention or for the development of new cancer therapies. In addition, the angiogenic activity presented by punicalagin in this study could contribute for the processes of tissue repairing and wound healing. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-10-19T16:08:06Z |
dc.date.issued.fl_str_mv |
2016-09-16 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CARNEIRO, C.C. Avaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivo. 2016. 123 f. Tese (Doutorado em Biologia) -Universidade Federal de Goiás, Goiânia, 2016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/6424 |
identifier_str_mv |
CARNEIRO, C.C. Avaliação das atividades genotóxica, antigenotóxica, citotóxica, anticitotóxica, angiogênica e antiangiogênica de elagitaninos utilizando ensaios in vitro e in vivo. 2016. 123 f. Tese (Doutorado em Biologia) -Universidade Federal de Goiás, Goiânia, 2016. |
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http://repositorio.bc.ufg.br/tede/handle/tede/6424 |
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por |
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openAccess |
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Universidade Federal de Goiás |
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UFG |
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Brasil |
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Instituto de Ciências Biológicas - ICB (RG) |
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Universidade Federal de Goiás |
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