Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás

Detalhes bibliográficos
Autor(a) principal: Cunha, Marielton dos Passos
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
dARK ID: ark:/38995/001300000866p
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/5162
Resumo: Dengue viruses (DENV) serotypes 1, 2, 3, and 4 have been causing yearly outbreaks in Brazil. Nevertheless, the population structure of the viruses transmitted in Goiás state is not well understood. In this study, we investigated the phylogenetic pattern of DENV samples identified in Goiânia, Goiás, Brazil during the 2012/2013 epidemic. Therefore, the entire region of the gene of the envelope (E) protein (1485bp) of 16 DENV-1 samples as well as partial region of this gene (363bp) of seven DENV-4 samples were sequenced. Phylogenetic analysis showed that DENV-1 belongs to the genotype V and presents itself divided into two clades, suggesting co-circulation of two distinct lineages in this region. Still, the molecular analyzes indicated a significant change in the amino acid level E348 position. For DENV-4, the sequences were segregate in a monophyletic group and are classified as genotype II American subclade. The molecular and phylogenetic analysis showed that the region suffered multiple introductions by dengue virus. This is the first report of co-circulation of two lineages of DENV-1, and the circulation of DENV-4 in Goiás state.
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spelling Fiaccadori, Fabíola Souzahttp://lattes.cnpq.br/0993842409303174Fiaccadori, Fabíola SouzaCoelho, Alexandre Siqueira GuedesCardoso, Divina das Dôres de Paulahttp://lattes.cnpq.br/8889654250506994Cunha, Marielton dos Passos2016-01-29T11:01:41Z2015-04-17CUNHA, M. P. Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás. 2015. 84 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/5162ark:/38995/001300000866pDengue viruses (DENV) serotypes 1, 2, 3, and 4 have been causing yearly outbreaks in Brazil. Nevertheless, the population structure of the viruses transmitted in Goiás state is not well understood. In this study, we investigated the phylogenetic pattern of DENV samples identified in Goiânia, Goiás, Brazil during the 2012/2013 epidemic. Therefore, the entire region of the gene of the envelope (E) protein (1485bp) of 16 DENV-1 samples as well as partial region of this gene (363bp) of seven DENV-4 samples were sequenced. Phylogenetic analysis showed that DENV-1 belongs to the genotype V and presents itself divided into two clades, suggesting co-circulation of two distinct lineages in this region. Still, the molecular analyzes indicated a significant change in the amino acid level E348 position. For DENV-4, the sequences were segregate in a monophyletic group and are classified as genotype II American subclade. The molecular and phylogenetic analysis showed that the region suffered multiple introductions by dengue virus. This is the first report of co-circulation of two lineages of DENV-1, and the circulation of DENV-4 in Goiás state.Os vírus dengue (DENV) sorotipos 1, 2, 3 e 4 tem causado surtos anuais no Brasil. No entanto, a estrutura populacional dos vírus transmitidos em Goiás não é bem compreendida. Neste estudo, investigamos o padrão filogenético de amostras do DENV identificadas em Goiânia, Goiás, Brasil, durante a epidemia de 2012/2013. Para isso, a região completa do gene codificante da proteína do envelope (E) (1485pb) de 16 amostras DENV-1 assim como a região parcial deste gene (363pb) de sete amostras DENV-4, foram sequenciadas. A análise filogenética mostrou que o DENV-1 pertence ao genótipo V e apresenta-se dividido em dois clados, sugerindo a cocirculação de duas linhagens distintas na região. Ainda, as análises moleculares indicaram uma alteração significativa na posição do aminoácido E348. Para DENV-4, as sequências segregaram em um grupo monofilético, sendo classificadas como genótipo II subclado americano. As análises moleculares e filogenéticas indicaram que a região sofreu múltiplas introduções pelo DENV. Este é o primeiro relato de cocirculação de duas linhagens de DENV-1, assim como circulação do DENV-4 no estado de Goiás.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2016-01-29T10:59:58Z No. of bitstreams: 2 Dissertação - Marielton dos Passos Cunha - 2015.pdf: 3794305 bytes, checksum: 63dd946c15b0a01f4a247e2bd60fa1e2 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-01-29T11:01:41Z (GMT) No. of bitstreams: 2 Dissertação - Marielton dos Passos Cunha - 2015.pdf: 3794305 bytes, checksum: 63dd946c15b0a01f4a247e2bd60fa1e2 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2016-01-29T11:01:41Z (GMT). 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dc.title.por.fl_str_mv Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás
dc.title.alternative.eng.fl_str_mv Phylogenetic characterization of isolates of dengue virus in Goiânia, Goiás
title Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás
spellingShingle Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás
Cunha, Marielton dos Passos
Dengue
Linhagens
Análise filogenética
Dengue virus
Lineages
Phylogenetic analysis
CIENCIAS BIOLOGICAS::PARASITOLOGIA
title_short Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás
title_full Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás
title_fullStr Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás
title_full_unstemmed Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás
title_sort Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás
author Cunha, Marielton dos Passos
author_facet Cunha, Marielton dos Passos
author_role author
dc.contributor.advisor1.fl_str_mv Fiaccadori, Fabíola Souza
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/0993842409303174
dc.contributor.referee1.fl_str_mv Fiaccadori, Fabíola Souza
dc.contributor.referee2.fl_str_mv Coelho, Alexandre Siqueira Guedes
dc.contributor.referee3.fl_str_mv Cardoso, Divina das Dôres de Paula
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8889654250506994
dc.contributor.author.fl_str_mv Cunha, Marielton dos Passos
contributor_str_mv Fiaccadori, Fabíola Souza
Fiaccadori, Fabíola Souza
Coelho, Alexandre Siqueira Guedes
Cardoso, Divina das Dôres de Paula
dc.subject.por.fl_str_mv Dengue
Linhagens
Análise filogenética
topic Dengue
Linhagens
Análise filogenética
Dengue virus
Lineages
Phylogenetic analysis
CIENCIAS BIOLOGICAS::PARASITOLOGIA
dc.subject.eng.fl_str_mv Dengue virus
Lineages
Phylogenetic analysis
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::PARASITOLOGIA
description Dengue viruses (DENV) serotypes 1, 2, 3, and 4 have been causing yearly outbreaks in Brazil. Nevertheless, the population structure of the viruses transmitted in Goiás state is not well understood. In this study, we investigated the phylogenetic pattern of DENV samples identified in Goiânia, Goiás, Brazil during the 2012/2013 epidemic. Therefore, the entire region of the gene of the envelope (E) protein (1485bp) of 16 DENV-1 samples as well as partial region of this gene (363bp) of seven DENV-4 samples were sequenced. Phylogenetic analysis showed that DENV-1 belongs to the genotype V and presents itself divided into two clades, suggesting co-circulation of two distinct lineages in this region. Still, the molecular analyzes indicated a significant change in the amino acid level E348 position. For DENV-4, the sequences were segregate in a monophyletic group and are classified as genotype II American subclade. The molecular and phylogenetic analysis showed that the region suffered multiple introductions by dengue virus. This is the first report of co-circulation of two lineages of DENV-1, and the circulation of DENV-4 in Goiás state.
publishDate 2015
dc.date.issued.fl_str_mv 2015-04-17
dc.date.accessioned.fl_str_mv 2016-01-29T11:01:41Z
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dc.identifier.citation.fl_str_mv CUNHA, M. P. Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás. 2015. 84 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015.
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dc.identifier.dark.fl_str_mv ark:/38995/001300000866p
identifier_str_mv CUNHA, M. P. Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás. 2015. 84 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015.
ark:/38995/001300000866p
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dc.publisher.program.fl_str_mv Programa de Pós-graduação em Biologia das Interações PH (IPTSP)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
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