AVALIAÇÃO DAS ATIVIDADES GENOTÓXICA E ANTIGENOTÓXICA DE DUGUETIA FURFURACEA EM BACTÉRIAS E CAMUNDONGOS

Detalhes bibliográficos
Autor(a) principal: SILVA, Carolina Ribeiro e
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
dARK ID: ark:/38995/00130000026ws
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tde/1277
Resumo: The plant Duguetia furfuracea (St. Hil.) Benth and Hook. f. (1862), belongs to the family Annonaceae, is popularly known as sofre&#8208;do&#8208;rim&#8208;quem&#8208;quer, araticum&#8208;do&#8208;cerrado and ata&#8208;do&#8208;mato. This plant occurres in several Brazilian states, as Amazonas, Bahia, Distrito Federal, Goiás, Minas Gerais, Mato Grosso, Mato Grosso do Sul and São Paulo. It is commonly used by people as anti&#8208;rheumatic and to cure renal colic. Previous studies have described therapeutic activity of this plant with trypanocidal, antiplasmodial and antiprotozoal effects. Due to the large utilization of this plant by the local people, the present work aimed to evaluate the genotoxic/mutagenic, antigenotoxic/antimutagenic and cytotoxic activities of lyophilized leaf extract of D. furfuracea by the lisogenic induction test (Inductest) and the mice bone marrow micronucleus assay (Micronucleus Test). The lisogenic induction test was performed according Moreau et al., (1976) using E. coli WP2s(&#955;) and RJF013 strains. To evaluate the genotoxic and toxic activities, the doses employed were 1 mg, 2 mg, 5 mg e 10 mg, while to evaluate the antigenotoxic action the doses were of 0,5 mg, 1 mg, 2 mg, 5 mg e 10 mg co&#8208;treated with 0,5 &#956;g de mitomycin C. Micronucleus test in the bone marrow of mice was performed according to Schmid (1975). To evaluate mutagenic activity, mice were treated with three different doses of plant extract (100, 200 and 300 mg/Kg body weight), to evaluate antimutagenic activity, mice were treated with the same doses co&#8208;administered with 4 mg/kg of mitomycin C. The genotoxic action was evaluated by the frequency of micronucleated polychromatic erythrocytes (MNPCE) and cytotoxicity was evaluated by the polychromatic and normochromatic erythrocytes ratio (PCE/NCE). The results obtained from the evaluation of mutagenicity of this plant showed that the extract of D. Furfuracea didn t increase significantly (p>0.05) the frequency of MNPCE compared to negative control. Cytotoxicity was evident in all applied doses (p<0.05). Concerning antimutagenicity, all doses of extract reduced significantly the frequency of MNPCE compared to the positive control group (p<0.05). In both tests, the results obtained demonstrated that the extract of D. furfuracea presented cytotoxicity; however, it didn t show genotoxic or mutagenic effects in bacteria and mice bone marrow respectively. In the other hand, it was observed a antigenotoxic and antimutagenic action.
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spelling CHEN, Lee Chenhttp://lattes.cnpq.br/4621907105842007http://lattes.cnpq.br/5491812153712081SILVA, Carolina Ribeiro e2014-07-29T15:16:34Z2010-04-152008-02-25SILVA, Carolina Ribeiro e. ASSESSMENT OF ACTIVITIES OF antigenotoxic genotoxic and Duguet furfuracea IN MICE AND BACTERIA. 2008. 118 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2008.http://repositorio.bc.ufg.br/tede/handle/tde/1277ark:/38995/00130000026wsThe plant Duguetia furfuracea (St. Hil.) Benth and Hook. f. (1862), belongs to the family Annonaceae, is popularly known as sofre&#8208;do&#8208;rim&#8208;quem&#8208;quer, araticum&#8208;do&#8208;cerrado and ata&#8208;do&#8208;mato. This plant occurres in several Brazilian states, as Amazonas, Bahia, Distrito Federal, Goiás, Minas Gerais, Mato Grosso, Mato Grosso do Sul and São Paulo. It is commonly used by people as anti&#8208;rheumatic and to cure renal colic. Previous studies have described therapeutic activity of this plant with trypanocidal, antiplasmodial and antiprotozoal effects. Due to the large utilization of this plant by the local people, the present work aimed to evaluate the genotoxic/mutagenic, antigenotoxic/antimutagenic and cytotoxic activities of lyophilized leaf extract of D. furfuracea by the lisogenic induction test (Inductest) and the mice bone marrow micronucleus assay (Micronucleus Test). The lisogenic induction test was performed according Moreau et al., (1976) using E. coli WP2s(&#955;) and RJF013 strains. To evaluate the genotoxic and toxic activities, the doses employed were 1 mg, 2 mg, 5 mg e 10 mg, while to evaluate the antigenotoxic action the doses were of 0,5 mg, 1 mg, 2 mg, 5 mg e 10 mg co&#8208;treated with 0,5 &#956;g de mitomycin C. Micronucleus test in the bone marrow of mice was performed according to Schmid (1975). To evaluate mutagenic activity, mice were treated with three different doses of plant extract (100, 200 and 300 mg/Kg body weight), to evaluate antimutagenic activity, mice were treated with the same doses co&#8208;administered with 4 mg/kg of mitomycin C. The genotoxic action was evaluated by the frequency of micronucleated polychromatic erythrocytes (MNPCE) and cytotoxicity was evaluated by the polychromatic and normochromatic erythrocytes ratio (PCE/NCE). The results obtained from the evaluation of mutagenicity of this plant showed that the extract of D. Furfuracea didn t increase significantly (p>0.05) the frequency of MNPCE compared to negative control. Cytotoxicity was evident in all applied doses (p<0.05). Concerning antimutagenicity, all doses of extract reduced significantly the frequency of MNPCE compared to the positive control group (p<0.05). In both tests, the results obtained demonstrated that the extract of D. furfuracea presented cytotoxicity; however, it didn t show genotoxic or mutagenic effects in bacteria and mice bone marrow respectively. In the other hand, it was observed a antigenotoxic and antimutagenic action.A planta Duguetia furfuracea (St. Hil.) Benth e Hook. f. (1862), pertence à família Annonaceae, é conhecida popularmente como sofre&#8208;do&#8208;rim&#8208;quem&#8208;quer, araticum&#8208;do&#8208;cerrado e ata&#8208;do&#8208;mato. Esta planta ocorre em vários estados brasileiros, como Amazonas, Bahia, Distrito Federal, Goiás, Minas Gerais, Mato Grosso, Mato Grosso do Sul e São Paulo. É comumente utilizada pela população como anti&#8208;reumática e para combater cólicas renais. Estudos anteriores têm descrito a atividade terapêutica da planta com ação tripanomicida, antiprotozoárica e antiplasmódica. Devido à grande utilização da planta D. furfuracea pela população, o presente trabalho teve como objetivo avaliar as atividades genotóxica/mutagênica, antigenotóxica/antimutagênica e citotóxica do extrato liofilizado de folhas de D. furfuracea pelo teste de indução do profago &#955; (Induteste) em cepas de E. coli e pelo teste do micronúcleo em medula óssea de camundongos (Teste do Micronúcleo). O teste de indução do profago &#955; foi realizado de acordo com Moreau et al. (1976), utilizando as cepas de E. coli WP2s(&#955;) e RJF013. Para a avaliação das atividades genotóxica e tóxica foram empregadas as doses de 1 mg, 2 mg, 5 mg e 10 mg, enquanto que na avaliação da ação antigenotóxica foram testadas as doses de 0,5 mg, 1 mg, 2 mg, 5 mg e 10 mg co&#8208;tratadas com 0,5 &#956;g de mitomicina C. O teste do micronúcleo em medula óssea de camundongos foi realizado de acordo com a metodologia de Schmid (1975). Para a avaliação da atividade mutagênica, os animais foram tratados com três diferentes doses do extrato da planta (100, 200 e 300 mg/Kg p.c.), enquanto que para a avaliação da atividade antimutagênica foram administradas as mesmas doses (100, 200 e 300 mg/Kg p.c.) concomitantemente de uma dose de 4 mg/Kg de mitomicina C. A genotoxicidade foi avaliada pela freqüência de eritrócitos policromáticos micronucleados (EPCMN) e a citotoxicidade foi analisada pela razão eritrócitos policromáticos e normocromáticos (EPC/ENC). Os resultados obtidos na avaliação da mutagenicidade mostraram que todas as doses do extrato de D. furfuracea não aumentaram significativamente o número de EPCMN (p>0,05), quando comparados com o grupo controle negativo. A citotoxicidade foi evidente em todas as doses testadas (p<0,05). Em relação à antimutagenicidade, todas as doses administradas reduziram significativamente a freqüência de EPCMN, em relação ao grupo controle positivo (p<0,05). Em ambos os testes, os resultados obtidos mostraram&#8208;se concordantes, nos quais o extrato de D. furfuracea apresentou atividade citotóxica; porém, não demonstrou atividade genotóxica em bactérias e ação mutagênica em camundongos. Por outro lado, foi observada ação antigenotóxica e antimutagênica.Made available in DSpace on 2014-07-29T15:16:34Z (GMT). No. of bitstreams: 1 Carol pdf.pdf: 1930051 bytes, checksum: b9c82e79bb7e104735c36e05b2702150 (MD5) Previous issue date: 2008-02-25application/pdfhttp://repositorio.bc.ufg.br/TEDE/retrieve/3966/Carol%20pdf.pdf.jpgporUniversidade Federal de GoiásMestrado em BiologiaUFGBRCiências BiolóicasDuguetia FurfuraceaGenotoxidadeteste do micronúcleoinduteste1.Araticum-seco - Duguetia furfuracea; 2. Medicina popularDuguetia furfuraceagenotoxicitymicronucleus testinductsCNPQ::CIENCIAS BIOLOGICAS::GENETICAAVALIAÇÃO DAS ATIVIDADES GENOTÓXICA E ANTIGENOTÓXICA DE DUGUETIA FURFURACEA EM BACTÉRIAS E CAMUNDONGOSASSESSMENT OF ACTIVITIES OF antigenotoxic genotoxic and Duguet furfuracea IN MICE AND BACTERIAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALCarol pdf.pdfapplication/pdf1930051http://repositorio.bc.ufg.br/tede/bitstreams/b2c844be-a828-415e-b444-e60020ecd538/downloadb9c82e79bb7e104735c36e05b2702150MD51TEXTCarol pdf.pdf.txtCarol pdf.pdf.txtExtracted Texttext/plain243435http://repositorio.bc.ufg.br/tede/bitstreams/96e11835-e20c-4ae6-9d65-b6426cf0b74c/downloadf5b8f54c28dad5691fe1b2d27c95ef24MD52THUMBNAILCarol pdf.pdf.jpgCarol pdf.pdf.jpgGenerated Thumbnailimage/jpeg3995http://repositorio.bc.ufg.br/tede/bitstreams/5735973e-eb27-429d-ba0d-08a7ac456f9d/downloadbef3c0123d13a45249e9ce7c69e1fbc5MD53tde/12772014-07-30 03:09:46.761open.accessoai:repositorio.bc.ufg.br:tde/1277http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2014-07-30T06:09:46Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)false
dc.title.por.fl_str_mv AVALIAÇÃO DAS ATIVIDADES GENOTÓXICA E ANTIGENOTÓXICA DE DUGUETIA FURFURACEA EM BACTÉRIAS E CAMUNDONGOS
dc.title.alternative.eng.fl_str_mv ASSESSMENT OF ACTIVITIES OF antigenotoxic genotoxic and Duguet furfuracea IN MICE AND BACTERIA
title AVALIAÇÃO DAS ATIVIDADES GENOTÓXICA E ANTIGENOTÓXICA DE DUGUETIA FURFURACEA EM BACTÉRIAS E CAMUNDONGOS
spellingShingle AVALIAÇÃO DAS ATIVIDADES GENOTÓXICA E ANTIGENOTÓXICA DE DUGUETIA FURFURACEA EM BACTÉRIAS E CAMUNDONGOS
SILVA, Carolina Ribeiro e
Duguetia Furfuracea
Genotoxidade
teste do micronúcleo
induteste
1.Araticum-seco - Duguetia furfuracea; 2. Medicina popular
Duguetia furfuracea
genotoxicity
micronucleus test
inducts
CNPQ::CIENCIAS BIOLOGICAS::GENETICA
title_short AVALIAÇÃO DAS ATIVIDADES GENOTÓXICA E ANTIGENOTÓXICA DE DUGUETIA FURFURACEA EM BACTÉRIAS E CAMUNDONGOS
title_full AVALIAÇÃO DAS ATIVIDADES GENOTÓXICA E ANTIGENOTÓXICA DE DUGUETIA FURFURACEA EM BACTÉRIAS E CAMUNDONGOS
title_fullStr AVALIAÇÃO DAS ATIVIDADES GENOTÓXICA E ANTIGENOTÓXICA DE DUGUETIA FURFURACEA EM BACTÉRIAS E CAMUNDONGOS
title_full_unstemmed AVALIAÇÃO DAS ATIVIDADES GENOTÓXICA E ANTIGENOTÓXICA DE DUGUETIA FURFURACEA EM BACTÉRIAS E CAMUNDONGOS
title_sort AVALIAÇÃO DAS ATIVIDADES GENOTÓXICA E ANTIGENOTÓXICA DE DUGUETIA FURFURACEA EM BACTÉRIAS E CAMUNDONGOS
author SILVA, Carolina Ribeiro e
author_facet SILVA, Carolina Ribeiro e
author_role author
dc.contributor.advisor1.fl_str_mv CHEN, Lee Chen
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4621907105842007
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5491812153712081
dc.contributor.author.fl_str_mv SILVA, Carolina Ribeiro e
contributor_str_mv CHEN, Lee Chen
dc.subject.por.fl_str_mv Duguetia Furfuracea
Genotoxidade
teste do micronúcleo
induteste
1.Araticum-seco - Duguetia furfuracea; 2. Medicina popular
topic Duguetia Furfuracea
Genotoxidade
teste do micronúcleo
induteste
1.Araticum-seco - Duguetia furfuracea; 2. Medicina popular
Duguetia furfuracea
genotoxicity
micronucleus test
inducts
CNPQ::CIENCIAS BIOLOGICAS::GENETICA
dc.subject.eng.fl_str_mv Duguetia furfuracea
genotoxicity
micronucleus test
inducts
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::GENETICA
description The plant Duguetia furfuracea (St. Hil.) Benth and Hook. f. (1862), belongs to the family Annonaceae, is popularly known as sofre&#8208;do&#8208;rim&#8208;quem&#8208;quer, araticum&#8208;do&#8208;cerrado and ata&#8208;do&#8208;mato. This plant occurres in several Brazilian states, as Amazonas, Bahia, Distrito Federal, Goiás, Minas Gerais, Mato Grosso, Mato Grosso do Sul and São Paulo. It is commonly used by people as anti&#8208;rheumatic and to cure renal colic. Previous studies have described therapeutic activity of this plant with trypanocidal, antiplasmodial and antiprotozoal effects. Due to the large utilization of this plant by the local people, the present work aimed to evaluate the genotoxic/mutagenic, antigenotoxic/antimutagenic and cytotoxic activities of lyophilized leaf extract of D. furfuracea by the lisogenic induction test (Inductest) and the mice bone marrow micronucleus assay (Micronucleus Test). The lisogenic induction test was performed according Moreau et al., (1976) using E. coli WP2s(&#955;) and RJF013 strains. To evaluate the genotoxic and toxic activities, the doses employed were 1 mg, 2 mg, 5 mg e 10 mg, while to evaluate the antigenotoxic action the doses were of 0,5 mg, 1 mg, 2 mg, 5 mg e 10 mg co&#8208;treated with 0,5 &#956;g de mitomycin C. Micronucleus test in the bone marrow of mice was performed according to Schmid (1975). To evaluate mutagenic activity, mice were treated with three different doses of plant extract (100, 200 and 300 mg/Kg body weight), to evaluate antimutagenic activity, mice were treated with the same doses co&#8208;administered with 4 mg/kg of mitomycin C. The genotoxic action was evaluated by the frequency of micronucleated polychromatic erythrocytes (MNPCE) and cytotoxicity was evaluated by the polychromatic and normochromatic erythrocytes ratio (PCE/NCE). The results obtained from the evaluation of mutagenicity of this plant showed that the extract of D. Furfuracea didn t increase significantly (p>0.05) the frequency of MNPCE compared to negative control. Cytotoxicity was evident in all applied doses (p<0.05). Concerning antimutagenicity, all doses of extract reduced significantly the frequency of MNPCE compared to the positive control group (p<0.05). In both tests, the results obtained demonstrated that the extract of D. furfuracea presented cytotoxicity; however, it didn t show genotoxic or mutagenic effects in bacteria and mice bone marrow respectively. In the other hand, it was observed a antigenotoxic and antimutagenic action.
publishDate 2008
dc.date.issued.fl_str_mv 2008-02-25
dc.date.available.fl_str_mv 2010-04-15
dc.date.accessioned.fl_str_mv 2014-07-29T15:16:34Z
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dc.identifier.citation.fl_str_mv SILVA, Carolina Ribeiro e. ASSESSMENT OF ACTIVITIES OF antigenotoxic genotoxic and Duguet furfuracea IN MICE AND BACTERIA. 2008. 118 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2008.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tde/1277
dc.identifier.dark.fl_str_mv ark:/38995/00130000026ws
identifier_str_mv SILVA, Carolina Ribeiro e. ASSESSMENT OF ACTIVITIES OF antigenotoxic genotoxic and Duguet furfuracea IN MICE AND BACTERIA. 2008. 118 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2008.
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dc.publisher.department.fl_str_mv Ciências Biolóicas
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