Avaliação do perfil de parasitemia por hemocultura seriada em indivíduos infectados cronicamente pelo Trypanosoma cruzi

• Autor(a) principal: Nascente, Flávia Martins
• Data de Publicação: 2010
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/001300000b1w8
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/8001
• topic: Doença de Chagas; Trypanosoma cruzi; Parasitemia e hemocultura seriada; CIENCIAS BIOLOGICAS::PARASITOLOGIA

Over a hundred years have passed since the discovery of Chagas disease and it is still a serious challenge to public health. Hemoculture has shown to be an important parasitological method aiming the isolation of Trypanosoma cruzi as well as in doubtful diagnosis and in the validation of the specific therapeutic efficacy. Its repetitive performance is another factor that increases its sensitivity. The aim of this study was to evaluate the parasitaemia profile through serial hemocultures from chronically T. cruzi infected individuals without previous treatment. 50 individuals from both genders (60% males) which had venous blood collected three fold with one month of minimum interval between the collections participated of this study. The technique used was of Chiari et al. & Luz et al. with LIT medium resulting in seven tubes per examination. Resulting in one performed hemoculture the sensitivity was of 32% in the first, 32% in the second sample and 34% in the third. When adding up the cumulative results of three samples the positivity increased to 58% (p=0,016). When we analyzed the parasitaemia profile of the positive hemocultures, 42% were of low parasitaemia (none of the three hemocultures were positive), 30% presented median parasitaemia (one hemoculture positive from the three performed), and 28% presented high parasitaemia (two or more positive hemocultures). When we related the parasitaemia level by hemoculture versus the number of positive tubes by hemocultures we found a direct relation between the parasitaemia and the number of positive tubes. Analyze the number of tubes positive blood culture proves to be sufficient to define the profile of individuals with parasitemia middle and high parasitemia, without the need for serial blood cultures. This can be expedited with the release of results when the clinician intends to make specific treatment.