Caracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviae
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/9180 |
Resumo: | Phosphorus is a nutritional limiting factor for plant growth but over 80% of the phosphorus applied directly to the soil becomes unavailable due to adsorption, precipitation or assimilation to organic phosphorus. One plant’s strategy to obtain phosphate from soils is mediated by the action of phosphatase enzymes, which catalyze hydrolysis of phosphate esters producing soluble phosphorus. In this study, a phosphate-solubilizing microorganism from Cerrado soil was used as phosphatase producer. The produced phosphatase was partially characterized and immobilized in different soil fractions. The microorganism that showed the highest phosphatase activity was identified as Pluralibacter gergoviae with a specific activity of 27.43 U/mg. The optimal activity of phosphatase produced by P. gergoviae was found at pH 5.2 and a temperature of 55°C. The effect of common inhibitors show that calcium and magnesium did not interfere in the phosphatase activity, whereas inorganic phosphate, ammonium and potassium slightly inhibited the enzyme activity. The best results of immobilization were obtained in the tests with silica and the organic fraction, which presented 44% of immobilization yield. Therefore, the immobilization of acid phosphatase produced by P.gergoviae in soil fractions might be an promising alternative for increasing the inorganic phosphate content in soil, which might contributes to the enhancing the agricultural productivity. |
id |
UFG-2_5f3d3c130eb5d265aec9e615eb3cb125 |
---|---|
oai_identifier_str |
oai:repositorio.bc.ufg.br:tede/9180 |
network_acronym_str |
UFG-2 |
network_name_str |
Repositório Institucional da UFG |
repository_id_str |
|
spelling |
Fernandes, Kátia Fláviahttp://lattes.cnpq.br/9737543228759171Fernandes, Kátia FláviaUlhoa, Cirano JoséCampos, Ivan Torres Nicolas dehttp://lattes.cnpq.br/4358533963974405Valencia Mejía, Erika2018-12-21T10:57:27Z2015-02-26VALENCIA MEJÍA, E. Caracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviae. 2015. 60 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Goiás, Goiânia, 2015http://repositorio.bc.ufg.br/tede/handle/tede/9180Phosphorus is a nutritional limiting factor for plant growth but over 80% of the phosphorus applied directly to the soil becomes unavailable due to adsorption, precipitation or assimilation to organic phosphorus. One plant’s strategy to obtain phosphate from soils is mediated by the action of phosphatase enzymes, which catalyze hydrolysis of phosphate esters producing soluble phosphorus. In this study, a phosphate-solubilizing microorganism from Cerrado soil was used as phosphatase producer. The produced phosphatase was partially characterized and immobilized in different soil fractions. The microorganism that showed the highest phosphatase activity was identified as Pluralibacter gergoviae with a specific activity of 27.43 U/mg. The optimal activity of phosphatase produced by P. gergoviae was found at pH 5.2 and a temperature of 55°C. The effect of common inhibitors show that calcium and magnesium did not interfere in the phosphatase activity, whereas inorganic phosphate, ammonium and potassium slightly inhibited the enzyme activity. The best results of immobilization were obtained in the tests with silica and the organic fraction, which presented 44% of immobilization yield. Therefore, the immobilization of acid phosphatase produced by P.gergoviae in soil fractions might be an promising alternative for increasing the inorganic phosphate content in soil, which might contributes to the enhancing the agricultural productivity.O fósforo é um fator nutricional limitante para o crescimento das plantas. No entanto, mais de 80% do fósforo aplicado diretamente sobre o solo torna-se indisponível devido à adsorção, precipitação ou assimilação. Uma estratégia da planta para obter fosfato de solos é mediada pela ação de enzimas do tipo fosfatase, que catalisam a hidrólise de ésteres de fosfato, tornando-os derivados de fósforo solúvel. Neste estudo, um microrganismo de solo de cerrado com a capacidade de solubilizar o fosfato foi utilizado como produtor de fosfatase. A fosfatase produzida foi parcialmente caracterizada e imobilizada em diferentes frações de solo. O microrganismo que apresentou a maior atividade de fosfatase foi identificado como Pluralibacter gergoviae, com uma atividade específica de 27,43 U mg -1 . A atividade ótima da fosfatase produzida por P. gergoviae foi encontrada a pH 5,2 e uma temperatura de 55 °C. No estudo do efeito de inibidores comuns foi observado que o cálcio e magnésio não interferem na atividade da fosfatase de P. gergoviae, enquanto que o fosfato inorgânico, o amônio e o potássio inibem ligeiramente a atividade da enzima. Os melhores resultados da imobilização foram obtidos nos ensaios com a sílica e a fração orgânica de solo, que apresentaram 44% de rendimento da imobilização. Por isso, a imobilização da fosfatase ácida produzida por P. gergoviae em frações de solo pode ser uma alternativa promissora para aumentar o teor de fosfato solúvel no solo, o que pode contribuir para a melhoria da produtividade agrícola.Submitted by Ana Caroline Costa (ana_caroline212@hotmail.com) on 2018-12-18T18:18:41Z No. of bitstreams: 2 Dissertação - Erika Valencia Mejía - 2015.pdf: 2339807 bytes, checksum: 8daa90cef634d98901d33e6a1e7dc758 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-12-21T10:57:27Z (GMT) No. of bitstreams: 2 Dissertação - Erika Valencia Mejía - 2015.pdf: 2339807 bytes, checksum: 8daa90cef634d98901d33e6a1e7dc758 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-12-21T10:57:27Z (GMT). No. of bitstreams: 2 Dissertação - Erika Valencia Mejía - 2015.pdf: 2339807 bytes, checksum: 8daa90cef634d98901d33e6a1e7dc758 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-02-26Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciências Biológicas (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMicrorganismosPluralibacter gergoviaeFosfataseSolubilização de fosfatoImobilizaçãoMicroorganismsPhosphatasePhosphate solubilizationImmobilizationCIENCIAS BIOLOGICAS::BIOQUIMICACaracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviaePartial characterization and immobilization of phosphatase produced by Pluralibacter gergoviaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-3362730732320261554600600600600-3872772117827373404893477990329266114-2555911436985713659reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://repositorio.bc.ufg.br/tede/bitstreams/bf415465-e1b6-4e6d-bbea-57dcf6bdc30d/downloadbd3efa91386c1718a7f26a329fdcb468MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.bc.ufg.br/tede/bitstreams/872a2ab3-ff60-42c2-9af2-592e7b4d7ad2/download4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://repositorio.bc.ufg.br/tede/bitstreams/772b9e45-883a-4430-af35-0c29d50012d5/downloadd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://repositorio.bc.ufg.br/tede/bitstreams/c0712714-baf4-4b69-9417-7022b99c8db7/downloadd41d8cd98f00b204e9800998ecf8427eMD54ORIGINALDissertação - Erika Valencia Mejía - 2015.pdfDissertação - Erika Valencia Mejía - 2015.pdfapplication/pdf2339807http://repositorio.bc.ufg.br/tede/bitstreams/36361157-8833-47c8-8ee8-0f591bbbd9e1/download8daa90cef634d98901d33e6a1e7dc758MD55tede/91802018-12-21 08:57:27.099http://creativecommons.org/licenses/by-nc-nd/4.0/Acesso Abertoopen.accessoai:repositorio.bc.ufg.br:tede/9180http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2018-12-21T10:57:27Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.eng.fl_str_mv |
Caracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviae |
dc.title.alternative.eng.fl_str_mv |
Partial characterization and immobilization of phosphatase produced by Pluralibacter gergoviae |
title |
Caracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviae |
spellingShingle |
Caracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviae Valencia Mejía, Erika Microrganismos Pluralibacter gergoviae Fosfatase Solubilização de fosfato Imobilização Microorganisms Phosphatase Phosphate solubilization Immobilization CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Caracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviae |
title_full |
Caracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviae |
title_fullStr |
Caracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviae |
title_full_unstemmed |
Caracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviae |
title_sort |
Caracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviae |
author |
Valencia Mejía, Erika |
author_facet |
Valencia Mejía, Erika |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Kátia Flávia |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9737543228759171 |
dc.contributor.referee1.fl_str_mv |
Fernandes, Kátia Flávia |
dc.contributor.referee2.fl_str_mv |
Ulhoa, Cirano José |
dc.contributor.referee3.fl_str_mv |
Campos, Ivan Torres Nicolas de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4358533963974405 |
dc.contributor.author.fl_str_mv |
Valencia Mejía, Erika |
contributor_str_mv |
Fernandes, Kátia Flávia Fernandes, Kátia Flávia Ulhoa, Cirano José Campos, Ivan Torres Nicolas de |
dc.subject.por.fl_str_mv |
Microrganismos Pluralibacter gergoviae Fosfatase Solubilização de fosfato Imobilização |
topic |
Microrganismos Pluralibacter gergoviae Fosfatase Solubilização de fosfato Imobilização Microorganisms Phosphatase Phosphate solubilization Immobilization CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Microorganisms Phosphatase Phosphate solubilization Immobilization |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Phosphorus is a nutritional limiting factor for plant growth but over 80% of the phosphorus applied directly to the soil becomes unavailable due to adsorption, precipitation or assimilation to organic phosphorus. One plant’s strategy to obtain phosphate from soils is mediated by the action of phosphatase enzymes, which catalyze hydrolysis of phosphate esters producing soluble phosphorus. In this study, a phosphate-solubilizing microorganism from Cerrado soil was used as phosphatase producer. The produced phosphatase was partially characterized and immobilized in different soil fractions. The microorganism that showed the highest phosphatase activity was identified as Pluralibacter gergoviae with a specific activity of 27.43 U/mg. The optimal activity of phosphatase produced by P. gergoviae was found at pH 5.2 and a temperature of 55°C. The effect of common inhibitors show that calcium and magnesium did not interfere in the phosphatase activity, whereas inorganic phosphate, ammonium and potassium slightly inhibited the enzyme activity. The best results of immobilization were obtained in the tests with silica and the organic fraction, which presented 44% of immobilization yield. Therefore, the immobilization of acid phosphatase produced by P.gergoviae in soil fractions might be an promising alternative for increasing the inorganic phosphate content in soil, which might contributes to the enhancing the agricultural productivity. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-26 |
dc.date.accessioned.fl_str_mv |
2018-12-21T10:57:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VALENCIA MEJÍA, E. Caracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviae. 2015. 60 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Goiás, Goiânia, 2015 |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/9180 |
identifier_str_mv |
VALENCIA MEJÍA, E. Caracterização parcial e imobilização de fosfatase produzida por Pluralibacter gergoviae. 2015. 60 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Goiás, Goiânia, 2015 |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/9180 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-3362730732320261554 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-3872772117827373404 |
dc.relation.cnpq.fl_str_mv |
893477990329266114 |
dc.relation.sponsorship.fl_str_mv |
-2555911436985713659 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Ciências Biológicas (ICB) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Ciências Biológicas - ICB (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/bf415465-e1b6-4e6d-bbea-57dcf6bdc30d/download http://repositorio.bc.ufg.br/tede/bitstreams/872a2ab3-ff60-42c2-9af2-592e7b4d7ad2/download http://repositorio.bc.ufg.br/tede/bitstreams/772b9e45-883a-4430-af35-0c29d50012d5/download http://repositorio.bc.ufg.br/tede/bitstreams/c0712714-baf4-4b69-9417-7022b99c8db7/download http://repositorio.bc.ufg.br/tede/bitstreams/36361157-8833-47c8-8ee8-0f591bbbd9e1/download |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e 8daa90cef634d98901d33e6a1e7dc758 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1798044369636294656 |