Estudo dos óleos naturais frente à Paracoccidioides spp.: atividade biológica de óleos de copaíba in natura e imobilizado em micelas

• Autor(a) principal: Miguel, Meire Ane Costa
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/00130000035hx
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/9655
• topic: Paracoccidioides spp.; Compostos naturais; Óleos; Micela; Antifúngicos; Paracoccidioides spp.; Natural compounds; Oils; Micelle; Antifungals; CIENCIAS BIOLOGICAS::MICROBIOLOGIA

Natural oils add therapeutic values and are commonly used as herbal medicines. Copaiba oil, sucupira and andiroba, the janauba latex, have been extracted from native Brazilian trees and have been used against various pathogens. In this work we used natural oils, such caryophylleneas janauba compound against latex and Paracoccidioides spp.. This genus corresponds to filamentous fungi that cause systemic mycosis Paracoccidioidomycosis (PCM) with records in Latin America, with the highest number of cases they are in Brazil. From these compounds it was determined on the cytotoxicity in Balb/c 3T3 cells and for the oils that had inhibited the growth fungal interaction with the antifungal agents amphotericin B, sulfamethoxazole-trimethoprim (SXT) and itraconazole were evaluated. In addition, the copaiba oil encapsulated in Pluronic® F-127 micelle (oil-micelle) is a promising compound, not yet elucidated in Paracoccidioides spp. was also tested. The minimal inhibitory concentration (MIC) and the cytotoxicity of the compounds were evidenced by the microdilution technique. The minimum fungicidal concentration (MFC) was determined by the growth in plaque by the checkerboard technique was used to assay the interaction of the oils with the antifungal agents. Copaiba, oil-micelle and sucupira oil exhibited fungicidal activity with MIC values of 62.5 μg /mL, 125 μg/mL and 250 μg/mL, respectively. Andiroba oil, janauba-latex β-caryophyllene and did not inhibit the growth of Paracoccidioides spp. at the concentrations tested. Copaiba, sucupira and oil-micelle showed no significant hemolytic activity and cytotoxicity in the Balb/c 3T3 cells at MIC concentrations. The combination of amphotericin B was synergistic with copaiba oil and oil-micelle, having a fractional inhibitory concentration of 0.75 μg/mL. Copaiba oil and oil-micelle have been shown to be promising for the treatment of PCM, as they inhibit fungal growth and have no cytotoxic or hemolytic effects. In addition, copaiba oil and oil-micelle in combination with amphotericin B demonstrated additive activity, whereas sucupira oil had no synergistic activity.