Identificação molecular, virulência e suscetibilidade aos antifúngicos das espécies do complexo Candida parapsilosis

Detalhes bibliográficos
Autor(a) principal: Ataídes, Fábio Silvestre
Data de Publicação: 2014
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFG
dARK ID: ark:/38995/0013000008dqh
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/5153
Resumo: The Candida species are associated with clinical manifestations that can characterize from superficial infections up to systemic involvement. Non-albicans species are considered emerging, and Candida parapsilosis is the main etiological agent in several casuistics. The secondary alcohol dehydrogenase (SADH) gene amplification and restriction fragment analysis by enzyme digestion BanI allows differentiation of C. parapsilosis in a species complex formed by C. parapsilosis sensu stricto, C. orthopsilosis and C. metapsilosis that may present behaviors different virulence and response to antifungal agents. Thus, this study aims to identify the species of the C. parapsilosis complex, obtained from mycology collection of the Mycology Laboratory of the Institute of Tropical Pathology and Public Health, Federal University of Goiás, using molecular methods, as well as the characterization of factors virulence and in vitro susceptibility to different antifungal agents. For the distinction between the species, was performed a polymerase chain reaction followed by digestion with restriction enzyme BanI. After the identification of 87 isolates from blood (54) and nails (33), the determination of virulence factors such as proteases, esterase, phospholipase, hemolysins, adhesion, biofilm formation and switching phenomenon were performed. The profile of in vitro susceptibility to amphotericin B, fluconazole, itraconazole, voriconazole, posaconazole and caspofungin were performed using the Etest® method. The results showed that C. parapsilosis sensu stricto is the most common in our region (78), followed by C.orthopsilosis (5) C. metapsilosis (4). There was no difference between the behavior of virulence by the three species, but noted that there is divergence in the susceptibility to antifungal agents. Isolates of C. parapsilosis sensu stricto were less susceptible to amphotericin B, itraconazole and caspofungin compared with the susceptibility patterns of C. orthopsilosis and C. metapsilosis. A percentage of 10.2% of the isolates of C. parapsilosis sensu stricto no were susceptible to caspofungin. This paper reports the first identification of species of the C. parapsilosis complex in the state of Goiás, Brazil, and shows the importance of determining antifungal susceptibility to appropriate therapy.
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spelling Silva, Maria do Rosário Rodrigueshttp://lattes.cnpq.br/7119226630434725Silva, Maria de Rosário RodriguesMelhem, Márcia de Souza CarvalhoCamplesi Junior, MiltonAmorim, Rosália Santos JesuinoSouza, Lucia Kioko Hasimoto ehttp://lattes.cnpq.br/9523959031041843Ataídes, Fábio Silvestre2016-01-28T11:43:48Z2014-08-26ATAÍDES, F. S. Identificação molecular, virulência e suscetibilidade aos antifúngicos das espécies do complexo Candida parapsilosis. 2014. 132 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2014.http://repositorio.bc.ufg.br/tede/handle/tede/5153ark:/38995/0013000008dqhThe Candida species are associated with clinical manifestations that can characterize from superficial infections up to systemic involvement. Non-albicans species are considered emerging, and Candida parapsilosis is the main etiological agent in several casuistics. The secondary alcohol dehydrogenase (SADH) gene amplification and restriction fragment analysis by enzyme digestion BanI allows differentiation of C. parapsilosis in a species complex formed by C. parapsilosis sensu stricto, C. orthopsilosis and C. metapsilosis that may present behaviors different virulence and response to antifungal agents. Thus, this study aims to identify the species of the C. parapsilosis complex, obtained from mycology collection of the Mycology Laboratory of the Institute of Tropical Pathology and Public Health, Federal University of Goiás, using molecular methods, as well as the characterization of factors virulence and in vitro susceptibility to different antifungal agents. For the distinction between the species, was performed a polymerase chain reaction followed by digestion with restriction enzyme BanI. After the identification of 87 isolates from blood (54) and nails (33), the determination of virulence factors such as proteases, esterase, phospholipase, hemolysins, adhesion, biofilm formation and switching phenomenon were performed. The profile of in vitro susceptibility to amphotericin B, fluconazole, itraconazole, voriconazole, posaconazole and caspofungin were performed using the Etest® method. The results showed that C. parapsilosis sensu stricto is the most common in our region (78), followed by C.orthopsilosis (5) C. metapsilosis (4). There was no difference between the behavior of virulence by the three species, but noted that there is divergence in the susceptibility to antifungal agents. Isolates of C. parapsilosis sensu stricto were less susceptible to amphotericin B, itraconazole and caspofungin compared with the susceptibility patterns of C. orthopsilosis and C. metapsilosis. A percentage of 10.2% of the isolates of C. parapsilosis sensu stricto no were susceptible to caspofungin. This paper reports the first identification of species of the C. parapsilosis complex in the state of Goiás, Brazil, and shows the importance of determining antifungal susceptibility to appropriate therapy.As espécies do gênero Candida estão relacionadas com manifestações clínicas que podem caracterizar desde infecções superficiais até um acometimento sistêmico. Espécies não-albicans, são consideradas emergentes, sendo que Candida parapsilosis é o principal agente etiológico em várias casuísticas. A amplificação do gene Secondary Alcohol Dehydrogenase (SADH) e a análise dos fragmentos de restrição pela enzima de digestão BanI, permite a diferenciação de C. parapsilosis em um complexo de espécies formado pela C. parapsilosis sensu stricto, C. orthopsilosis e C. metapsilosis, que podem apresentar comportamentos diferentes de virulência e resposta aos antifúngicos. Deste modo, este trabalho se propõe a identificar as espécies pertencentes ao complexo C. parapsilosis, obtidas da micoteca do Laboratório de Micologia do Instituto de Patologia Tropical e Saúde Pública da Universidade Federal de Goiás, usando métodos moleculares, bem como a caracterização dos fatores de virulência, e perfil de suscetibilidade in vitro a diferentes agentes antifúngicos. Para a distinção entre as espécies foi realizado a reação em cadeia da polimerase (PCR) seguido de digestão pela enzima de restrição BanI. Após a identificação de 87 isolados obtidos de sangue (54) e de unhas (33), foi realizada a determinação dos fatores de virulência como proteinases, esterase, fosfolipase, hemolisinas, aderência, formação de biofilme e fenômeno switching. O perfil de suscetibilidade a anfotericina B, fluconazol, itraconazol, voriconazol, posaconazol e caspofungina foi realizado usando-se o método de Etest®. Os resultados mostraram que C. parapsilosis sensu stricto é a mais frequente na nossa região (78), seguido de C. orthopsilosis (5) e C. metapsilosis (4). Não se verificou diferença entre o comportamento de virulência pelas três espécies, mas foi observado que há divergência na suscetibilidade aos agentes antifúngicos. Isolados de C. parapsilosis sensu stricto foram menos suscetíveis a anfotericina B, itraconazol e caspofungina quando comparados com os padrões de suscetibilidade de C. orthopsilosis e C. metapsilosis. Um percentual de 10,2% dos isolados de C. parapsilosis sensu stricto apresentaram-se não suscetíveis a caspofungina. Este trabalho relata pela primeira vez a identificação de espécies do complexo C. parapsilosis no estado de Goiás, Brasil, e mostra a importância de determinação de suscetibilidade antifúngica para uma terapia adequada.Submitted by Cláudia Bueno (claudiamoura18@gmail.com) on 2016-01-27T16:27:50Z No. of bitstreams: 2 Tese - Fábio Silvestre Ataides - 2014.pdf: 2285867 bytes, checksum: 7c53025bcd16ddc4bc411b6f0415905a (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-01-28T11:43:48Z (GMT) No. of bitstreams: 2 Tese - Fábio Silvestre Ataides - 2014.pdf: 2285867 bytes, checksum: 7c53025bcd16ddc4bc411b6f0415905a (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2016-01-28T11:43:48Z (GMT). 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dc.title.por.fl_str_mv Identificação molecular, virulência e suscetibilidade aos antifúngicos das espécies do complexo Candida parapsilosis
dc.title.alternative.eng.fl_str_mv Molecular identification, virulence and antifungal susceptibility of Candida parapsilosis complex species
title Identificação molecular, virulência e suscetibilidade aos antifúngicos das espécies do complexo Candida parapsilosis
spellingShingle Identificação molecular, virulência e suscetibilidade aos antifúngicos das espécies do complexo Candida parapsilosis
Ataídes, Fábio Silvestre
Complexo Candida parapsilosis
Identificação molecular
Virulência
Molecular identification
Virulence
Complex Candida parapsilosis
MICROBIOLOGIA::MICROBIOLOGIA APLICADA
title_short Identificação molecular, virulência e suscetibilidade aos antifúngicos das espécies do complexo Candida parapsilosis
title_full Identificação molecular, virulência e suscetibilidade aos antifúngicos das espécies do complexo Candida parapsilosis
title_fullStr Identificação molecular, virulência e suscetibilidade aos antifúngicos das espécies do complexo Candida parapsilosis
title_full_unstemmed Identificação molecular, virulência e suscetibilidade aos antifúngicos das espécies do complexo Candida parapsilosis
title_sort Identificação molecular, virulência e suscetibilidade aos antifúngicos das espécies do complexo Candida parapsilosis
author Ataídes, Fábio Silvestre
author_facet Ataídes, Fábio Silvestre
author_role author
dc.contributor.advisor1.fl_str_mv Silva, Maria do Rosário Rodrigues
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7119226630434725
dc.contributor.referee1.fl_str_mv Silva, Maria de Rosário Rodrigues
dc.contributor.referee2.fl_str_mv Melhem, Márcia de Souza Carvalho
dc.contributor.referee3.fl_str_mv Camplesi Junior, Milton
dc.contributor.referee4.fl_str_mv Amorim, Rosália Santos Jesuino
dc.contributor.referee5.fl_str_mv Souza, Lucia Kioko Hasimoto e
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9523959031041843
dc.contributor.author.fl_str_mv Ataídes, Fábio Silvestre
contributor_str_mv Silva, Maria do Rosário Rodrigues
Silva, Maria de Rosário Rodrigues
Melhem, Márcia de Souza Carvalho
Camplesi Junior, Milton
Amorim, Rosália Santos Jesuino
Souza, Lucia Kioko Hasimoto e
dc.subject.por.fl_str_mv Complexo Candida parapsilosis
Identificação molecular
Virulência
topic Complexo Candida parapsilosis
Identificação molecular
Virulência
Molecular identification
Virulence
Complex Candida parapsilosis
MICROBIOLOGIA::MICROBIOLOGIA APLICADA
dc.subject.eng.fl_str_mv Molecular identification
Virulence
Complex Candida parapsilosis
dc.subject.cnpq.fl_str_mv MICROBIOLOGIA::MICROBIOLOGIA APLICADA
description The Candida species are associated with clinical manifestations that can characterize from superficial infections up to systemic involvement. Non-albicans species are considered emerging, and Candida parapsilosis is the main etiological agent in several casuistics. The secondary alcohol dehydrogenase (SADH) gene amplification and restriction fragment analysis by enzyme digestion BanI allows differentiation of C. parapsilosis in a species complex formed by C. parapsilosis sensu stricto, C. orthopsilosis and C. metapsilosis that may present behaviors different virulence and response to antifungal agents. Thus, this study aims to identify the species of the C. parapsilosis complex, obtained from mycology collection of the Mycology Laboratory of the Institute of Tropical Pathology and Public Health, Federal University of Goiás, using molecular methods, as well as the characterization of factors virulence and in vitro susceptibility to different antifungal agents. For the distinction between the species, was performed a polymerase chain reaction followed by digestion with restriction enzyme BanI. After the identification of 87 isolates from blood (54) and nails (33), the determination of virulence factors such as proteases, esterase, phospholipase, hemolysins, adhesion, biofilm formation and switching phenomenon were performed. The profile of in vitro susceptibility to amphotericin B, fluconazole, itraconazole, voriconazole, posaconazole and caspofungin were performed using the Etest® method. The results showed that C. parapsilosis sensu stricto is the most common in our region (78), followed by C.orthopsilosis (5) C. metapsilosis (4). There was no difference between the behavior of virulence by the three species, but noted that there is divergence in the susceptibility to antifungal agents. Isolates of C. parapsilosis sensu stricto were less susceptible to amphotericin B, itraconazole and caspofungin compared with the susceptibility patterns of C. orthopsilosis and C. metapsilosis. A percentage of 10.2% of the isolates of C. parapsilosis sensu stricto no were susceptible to caspofungin. This paper reports the first identification of species of the C. parapsilosis complex in the state of Goiás, Brazil, and shows the importance of determining antifungal susceptibility to appropriate therapy.
publishDate 2014
dc.date.issued.fl_str_mv 2014-08-26
dc.date.accessioned.fl_str_mv 2016-01-28T11:43:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
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dc.identifier.citation.fl_str_mv ATAÍDES, F. S. Identificação molecular, virulência e suscetibilidade aos antifúngicos das espécies do complexo Candida parapsilosis. 2014. 132 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2014.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/5153
dc.identifier.dark.fl_str_mv ark:/38995/0013000008dqh
identifier_str_mv ATAÍDES, F. S. Identificação molecular, virulência e suscetibilidade aos antifúngicos das espécies do complexo Candida parapsilosis. 2014. 132 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2014.
ark:/38995/0013000008dqh
url http://repositorio.bc.ufg.br/tede/handle/tede/5153
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 6085308344741430434
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv -7769011444564556288
dc.relation.cnpq.fl_str_mv -203884118251241698
dc.relation.sponsorship.fl_str_mv -2555911436985713659
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFG
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
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reponame_str Repositório Institucional da UFG
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repository.name.fl_str_mv Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv tasesdissertacoes.bc@ufg.br
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