Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte

Detalhes bibliográficos
Autor(a) principal: Figueira, Samantha Verdi
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/6642
Resumo: The present study was developted with the main object to evaluate the invasive ability, persitence and behavior of Salmonella Schwarzengrund in embryos and broiler chicks with one day of birth. Initially, 260 fertile eggs from broiler chicken were incubated e randomly distributed onto three different incubators. In the nineteenth day post incubation, the eggs were submited to candling and at the time 40 eggs were eliminated. The remaining eggs were distrubuted and 50 eggs were inoculated with 0,2 mL of sterile buffered saline 0,85% trough the air chamber and 50 eggs were inoculated with 1,5 x 104 UFC/0,2mL of Salmonella Schwarzengrund trough the air chamber, 100 were incubated without treatment. After the eclosion, the one day-old chicks were equally distributed in four treatments, discriminated well below: T1 – feed control – 30 chicks from incubator number 1, and received 0,1 mL of sterile buffered saline 0,85% per g of feed; T2 – feed inoculated - 30 chicks from incubator number 1, received feed with 5,3 x 10 4/0,1mL/g of Salmonella Schwarzengrund in buffered saline 0,85% per g de of feed until the seventh day-old; T3- inoculated control trough air chamber -30 chicks from inoculated embryos with 0,2 mL of sterile buffered saline 0,85% trough the air chamber; T4- inoculated trough air chamber - 30 chicks from inoculated embryos with 1,5 x 104 UFC/0,2mL de Salmonella Schwarzengrund in the air chamber. Salmonella Schwarzengrund was able to cause embryo mortality, enteric colonization, negatively influenced the weight gain, septicemic and enteric signs, with no correlation between the inoculation way. The bidrs shown clinical signs seen as feces adered to cloaca,diarrhea, prostration, decreased appetite, growth retardation and sleepness. The contamination determined higher weight and length of intestinal and liver with 25 days.Therefore is conclude that Salmonella Schwarzengrund is able to invade and persist in organs of inoculated air chamber embryo and one day old chicks contaminated feed.
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spelling Andrade, Maria Auxiliadorahttp://lattes.cnpq.br/9441751521255467Rezende, Cíntia Silva Minafra ehttp://lattes.cnpq.br/5841210447886226Strighini, José Henriquehttp://lattes.cnpq.br/8505634095383289Andrade, Maria AuxiliadoraBarbosa, Silvia MinharroCafé, Marcos Barcelloshttp://lattes.cnpq.br/3212246712633622Figueira, Samantha Verdi2016-12-26T14:34:33Z2014-08-07FIGUEIRA, S. V. Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte. 2014. 51 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016.http://repositorio.bc.ufg.br/tede/handle/tede/6642The present study was developted with the main object to evaluate the invasive ability, persitence and behavior of Salmonella Schwarzengrund in embryos and broiler chicks with one day of birth. Initially, 260 fertile eggs from broiler chicken were incubated e randomly distributed onto three different incubators. In the nineteenth day post incubation, the eggs were submited to candling and at the time 40 eggs were eliminated. The remaining eggs were distrubuted and 50 eggs were inoculated with 0,2 mL of sterile buffered saline 0,85% trough the air chamber and 50 eggs were inoculated with 1,5 x 104 UFC/0,2mL of Salmonella Schwarzengrund trough the air chamber, 100 were incubated without treatment. After the eclosion, the one day-old chicks were equally distributed in four treatments, discriminated well below: T1 – feed control – 30 chicks from incubator number 1, and received 0,1 mL of sterile buffered saline 0,85% per g of feed; T2 – feed inoculated - 30 chicks from incubator number 1, received feed with 5,3 x 10 4/0,1mL/g of Salmonella Schwarzengrund in buffered saline 0,85% per g de of feed until the seventh day-old; T3- inoculated control trough air chamber -30 chicks from inoculated embryos with 0,2 mL of sterile buffered saline 0,85% trough the air chamber; T4- inoculated trough air chamber - 30 chicks from inoculated embryos with 1,5 x 104 UFC/0,2mL de Salmonella Schwarzengrund in the air chamber. Salmonella Schwarzengrund was able to cause embryo mortality, enteric colonization, negatively influenced the weight gain, septicemic and enteric signs, with no correlation between the inoculation way. The bidrs shown clinical signs seen as feces adered to cloaca,diarrhea, prostration, decreased appetite, growth retardation and sleepness. The contamination determined higher weight and length of intestinal and liver with 25 days.Therefore is conclude that Salmonella Schwarzengrund is able to invade and persist in organs of inoculated air chamber embryo and one day old chicks contaminated feed.O presente estudo foi desenvolvido com o objetivo de investigar a capacidade invasiva e a persistência da Salmonella Schwarzengrund em embriões e pintos de corte de um dia. Foram incubados, inteiramente ao acaso, 260 ovos férteis de frangos de corte em três incubadoras distintas. Com 19 dias de incubação fez-se a ovoscopia e, 40 ovos foram eliminados. O restante dos ovos foram distribuídos da seguinte maneira: 50 ovos foram inoculados com 0,2 mL de solução salina tamponada esterilizada 0,85% via câmara de ar, 50 ovos foram inoculados com 1,5 x 104 UFC/0,2mL de Salmonella Schwarzengrund na câmara de ar e 100 foram incubados sem nenhum inóculo, para posterior inoculação nos pintos de um dia. Após a eclosão, os pintos de um dia foram distribuídos igualmente em quatro tratamentos, assim discriminados: T1 - controle da ração - constituído de 30 pintos livres de Salmonella, e receberam ração inoculada com 0,1 mL solução salina tamponada a 0, 85% por g de ração; T2 – inoculado ração - constituído de 30 pintos oriundos da incubadora 1, receberam ração com 5,3 x 10 4/0,1mL/g de Salmonella Schwarzengrund em solução salina tamponada 0, 85% por grama de ração até o sétimo dia de vida; T3- pintos controle oriundos da inoculação via câmara de ar ainda embriões -30 pintos oriundos de embriões inoculados com 0,2 mL de solução salina 0,85% tamponada, esterilizada na câmara de ar; T4- pintos contaminados câmara de ar ainda embriões- 30 pintos oriundos de embriões inoculados com 1,5 x 104 UFC/0,2mL de Salmonella Schwarzengrund na câmara de ar. Salmonella Schwarzengrund foi capaz de causar a mortalidade embrionária, colonizar o intestino, influenciar negativamente o ganho de peso, determinar quadro septicêmico e entérico independente da via de inoculação. As aves apresentaram sinais clínicos como fezes aderidas à cloaca, diarreia, prostração, apetite diminuído, retardo no crescimento e sonolência. A contaminação determinou maior peso e comprimento do intestino e fígado com 25 dias. Conclui-se que Salmonella Schwarzengrund possui a capacidade invasiva e de persistência em aves oriundas da inoculação da câmera de ar e em pintos de um dia por ração contaminada.Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2016-12-19T10:48:39Z No. of bitstreams: 2 Dissertação - Samantha Verdi Figueira - 2014.pdf: 1208888 bytes, checksum: 9e0b3cc451a0348f01e26e1d1e9fabf3 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-12-26T14:34:33Z (GMT) No. of bitstreams: 2 Dissertação - Samantha Verdi Figueira - 2014.pdf: 1208888 bytes, checksum: 9e0b3cc451a0348f01e26e1d1e9fabf3 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2016-12-26T14:34:33Z (GMT). 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dc.title.por.fl_str_mv Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte
dc.title.alternative.eng.fl_str_mv Experimental inoculation of Salmonella enterica Serovar Schwarzengrund in embryos and broilers chicks
title Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte
spellingShingle Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte
Figueira, Samantha Verdi
Salmonelose
Ovos férteis
Ração
Vias de transmissão
Salmonelosis
Fertile eggs
Feed
Transmission paths
MEDICINA VETERINARIA::PATOLOGIA ANIMAL
title_short Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte
title_full Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte
title_fullStr Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte
title_full_unstemmed Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte
title_sort Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte
author Figueira, Samantha Verdi
author_facet Figueira, Samantha Verdi
author_role author
dc.contributor.advisor1.fl_str_mv Andrade, Maria Auxiliadora
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9441751521255467
dc.contributor.advisor-co1.fl_str_mv Rezende, Cíntia Silva Minafra e
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/5841210447886226
dc.contributor.advisor-co2.fl_str_mv Strighini, José Henrique
dc.contributor.advisor-co2Lattes.fl_str_mv http://lattes.cnpq.br/8505634095383289
dc.contributor.referee1.fl_str_mv Andrade, Maria Auxiliadora
dc.contributor.referee2.fl_str_mv Barbosa, Silvia Minharro
dc.contributor.referee3.fl_str_mv Café, Marcos Barcellos
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3212246712633622
dc.contributor.author.fl_str_mv Figueira, Samantha Verdi
contributor_str_mv Andrade, Maria Auxiliadora
Rezende, Cíntia Silva Minafra e
Strighini, José Henrique
Andrade, Maria Auxiliadora
Barbosa, Silvia Minharro
Café, Marcos Barcellos
dc.subject.por.fl_str_mv Salmonelose
Ovos férteis
Ração
Vias de transmissão
topic Salmonelose
Ovos férteis
Ração
Vias de transmissão
Salmonelosis
Fertile eggs
Feed
Transmission paths
MEDICINA VETERINARIA::PATOLOGIA ANIMAL
dc.subject.eng.fl_str_mv Salmonelosis
Fertile eggs
Feed
Transmission paths
dc.subject.cnpq.fl_str_mv MEDICINA VETERINARIA::PATOLOGIA ANIMAL
description The present study was developted with the main object to evaluate the invasive ability, persitence and behavior of Salmonella Schwarzengrund in embryos and broiler chicks with one day of birth. Initially, 260 fertile eggs from broiler chicken were incubated e randomly distributed onto three different incubators. In the nineteenth day post incubation, the eggs were submited to candling and at the time 40 eggs were eliminated. The remaining eggs were distrubuted and 50 eggs were inoculated with 0,2 mL of sterile buffered saline 0,85% trough the air chamber and 50 eggs were inoculated with 1,5 x 104 UFC/0,2mL of Salmonella Schwarzengrund trough the air chamber, 100 were incubated without treatment. After the eclosion, the one day-old chicks were equally distributed in four treatments, discriminated well below: T1 – feed control – 30 chicks from incubator number 1, and received 0,1 mL of sterile buffered saline 0,85% per g of feed; T2 – feed inoculated - 30 chicks from incubator number 1, received feed with 5,3 x 10 4/0,1mL/g of Salmonella Schwarzengrund in buffered saline 0,85% per g de of feed until the seventh day-old; T3- inoculated control trough air chamber -30 chicks from inoculated embryos with 0,2 mL of sterile buffered saline 0,85% trough the air chamber; T4- inoculated trough air chamber - 30 chicks from inoculated embryos with 1,5 x 104 UFC/0,2mL de Salmonella Schwarzengrund in the air chamber. Salmonella Schwarzengrund was able to cause embryo mortality, enteric colonization, negatively influenced the weight gain, septicemic and enteric signs, with no correlation between the inoculation way. The bidrs shown clinical signs seen as feces adered to cloaca,diarrhea, prostration, decreased appetite, growth retardation and sleepness. The contamination determined higher weight and length of intestinal and liver with 25 days.Therefore is conclude that Salmonella Schwarzengrund is able to invade and persist in organs of inoculated air chamber embryo and one day old chicks contaminated feed.
publishDate 2014
dc.date.issued.fl_str_mv 2014-08-07
dc.date.accessioned.fl_str_mv 2016-12-26T14:34:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
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dc.identifier.citation.fl_str_mv FIGUEIRA, S. V. Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte. 2014. 51 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/6642
identifier_str_mv FIGUEIRA, S. V. Inoculação experimental de Salmonella entérica Sorovar Schwarzengrund em embriões e pintos de corte. 2014. 51 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016.
url http://repositorio.bc.ufg.br/tede/handle/tede/6642
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language por
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600
600
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dc.relation.cnpq.fl_str_mv 5069770875075328004
dc.relation.sponsorship.fl_str_mv -2555911436985713659
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dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Ciência Animal (EVZ)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Escola de Veterinária e Zootecnia - EVZ (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
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MD5
repository.name.fl_str_mv Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv tasesdissertacoes.bc@ufg.br
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