Avaliação da toxicidade aguda oral, atividades antinociceptiva e anti-inflamatória da crotamina isolada da peçonha de Crotalus durissus collilineatus em camundongos

• Autor(a) principal: Moreira, Lorena Alves
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/8747
• topic: Crotalus durissus collilineatus; Crotamina; Analgesia; Inflamação; Toxicidade; Crotamine; Antinociceptive effect; Inflammation; Toxicity; CIENCIAS DA SAUDE::FARMACIA

Introduction: Crotamine is a low molecular weight cationic polypeptide (4.8 kDa), isolated from rattlesnake venom. In the previous study the presence of this toxin in the snake venom Crotalus durissus collilineatus was responsible for anti-edematogenic activity, suggesting its anti-inflammatory properties. Pain and inflammation are present mechanisms in several pathologies, being important the search for new compounds candidates for effective drugs and with less adverse effects than those existing in the market for such treatments. Objective: To determine acute oral toxicity of crotamine isolated from snake venom C. d. collilineatus was evaluated and its effects in experimental models of pain and inflammation in mice was investigated. Methodology: To evaluate acute oral toxicity, the up and down procedure and hippocratic screening was performed. For the nociceptive and inflammation assays, acetic acid-induced abdominal writhing, formalin-induced pain, ear edema induced by croton oil and pleurisy induced by carrageenan were performed. Results and Discussion: Crotamine did not cause lethality or signs of intoxication in the dose range of 0.34 to 10.88 mg/kg. In the abdominal writhing test, treatments at doses of 0.08, 0.16 and 0.32 mg/kg p.o. reduced the number of writhings in relation to the control by 34, 57 and 74 %, respectively, showing a dose-dependent trend. In the formalin-induced pain test it was observed that crotamine (0.16 mg/kg p.o.) reduced reactivity to pain in the neurogenic phase in 44.8% and in the inflammatory phase in 59.7%, suggesting antinociceptive activity. In addition, crotamine (0.16 mg/kg p.o.) was able to reduce ear edema induced by croton oil by 77 %, showing antidematogenic activity and reduced in 52.3 % the number of total leukocytes in the pleurisy test, reducing both neutrophil and mononuclear cells migration, thus exhibiting antimigratory activity. Conclusion: Crotamine did not induce a toxic or lethal effect at the doses tested. The reductions in writhings, reactivity to pain, ear edema and leukocytes migration with administration of crotamine were significant, suggesting that it has antinociceptive and anti-inflammatory activities.