Listeria spp. e L. monocytogenes em carne bovina refrigerada e embalada a vácuo, equipamentos e ambiente de matadouros frigoríficos
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tde/928 |
Resumo: | Intending to stipulate the parcel of Listeria spp. and L. monocytogenes in several sources, using the PCR techicic, 360 samples were analyzed in the period between november 2006 and february 2008, proceeding from 25 commercial abattoirs of five companies all licensed to export from some Brazilian States. Primers U1/LI1 for Listeria spp. and LM1/LM2 and LL5/LL6 for L. monocytogenes. Listeria spp. was detected in 19,72% of the samples, with percentage of 23% in the bone rooms, cold chamber and half carcass, 15% in beef cuts and 10% in the slaughter rooms. In the slaughterhouses it was detected in 19,42% in the A, 17,72% in the B, 22,95% in the C and 20% in the D and E. Analyzing the states, 16,34% in São Paulo, 23,94% in Mato Grosso do Sul, 14,06% in Goiás, 32% in Mato Grosso, 27,27% in Bahia and 14,28% in Pará were positive for its genus. No matter the pair of ‟primers‟‟ used for amplication, L. monocytogenes were detected in 14,72% of the samples, 20% in cold chambers, 15,83% in bone rooms, 13.33% in halfcarcass and in beef cuts and 10% in slaughter rooms. In the slaughterhouse A, 12,57% of the samples were detected positive‟, 11,39% in B, 21,31% in C and 13,33% in D e E. The bacterium was detected in São Paulo in 12,42% of positive samples, 14,08% in Mato Grosso do Sul, 12,30% in Goiás, 24%, in Mato Grosso, 27,27% in Bahia and 14,28% in Pará. The pair of primer LM1/LM2 revealed better efficience than the LL5/LL6 and although this last one has presented low efficiency to detection, the total of positive samples of L. monocytogenes of this research only was possible by the association of both. Listeria spp. and L. monocytogenes are largely propagated in the sources and studied places, and, therefore, disseminated in all slaughterhouses and analyzed States, excepting Minas Gerais. The results of this research revealed the necessariness of adoption of urgent providences about the control of the pathogen in the bovine slaughterhouses in Brazil and must alert the possibility of occurrence of outbreak of listeriosis of nutritious transmission, considering the occurrence and the frequency repetition here verified in half-carcass and beef cuts |
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NUNES, Iolanda Aparecidahttp://lattes.cnpq.br/2555738917312914MESQUITA, Albenones José dehttp://lattes.cnpq.br/3180029815183858MEIRELES, Marcelo Vasconceloshttp://lattes.cnpq.br/0903513897615274http://lattes.cnpq.br/0501694555123594FRANÇA, Leonardo2014-07-29T15:07:51Z2010-02-042008-08-28FRANÇA, Leonardo. Listeria spp. and L. monocytogenes in vacuum-packed chilled beef, equipment, environment of slaughterhouses. 2008. 93 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2008.http://repositorio.bc.ufg.br/tede/handle/tde/928ark:/38995/001300000cr1mIntending to stipulate the parcel of Listeria spp. and L. monocytogenes in several sources, using the PCR techicic, 360 samples were analyzed in the period between november 2006 and february 2008, proceeding from 25 commercial abattoirs of five companies all licensed to export from some Brazilian States. Primers U1/LI1 for Listeria spp. and LM1/LM2 and LL5/LL6 for L. monocytogenes. Listeria spp. was detected in 19,72% of the samples, with percentage of 23% in the bone rooms, cold chamber and half carcass, 15% in beef cuts and 10% in the slaughter rooms. In the slaughterhouses it was detected in 19,42% in the A, 17,72% in the B, 22,95% in the C and 20% in the D and E. Analyzing the states, 16,34% in São Paulo, 23,94% in Mato Grosso do Sul, 14,06% in Goiás, 32% in Mato Grosso, 27,27% in Bahia and 14,28% in Pará were positive for its genus. No matter the pair of ‟primers‟‟ used for amplication, L. monocytogenes were detected in 14,72% of the samples, 20% in cold chambers, 15,83% in bone rooms, 13.33% in halfcarcass and in beef cuts and 10% in slaughter rooms. In the slaughterhouse A, 12,57% of the samples were detected positive‟, 11,39% in B, 21,31% in C and 13,33% in D e E. The bacterium was detected in São Paulo in 12,42% of positive samples, 14,08% in Mato Grosso do Sul, 12,30% in Goiás, 24%, in Mato Grosso, 27,27% in Bahia and 14,28% in Pará. The pair of primer LM1/LM2 revealed better efficience than the LL5/LL6 and although this last one has presented low efficiency to detection, the total of positive samples of L. monocytogenes of this research only was possible by the association of both. Listeria spp. and L. monocytogenes are largely propagated in the sources and studied places, and, therefore, disseminated in all slaughterhouses and analyzed States, excepting Minas Gerais. The results of this research revealed the necessariness of adoption of urgent providences about the control of the pathogen in the bovine slaughterhouses in Brazil and must alert the possibility of occurrence of outbreak of listeriosis of nutritious transmission, considering the occurrence and the frequency repetition here verified in half-carcass and beef cutsObjetivando determinar a distribuição de Listeria spp. e L. monocytogenes em fontes diversas, pela técnica de PCR, foram analisadas 360 amostras no período de novembro de 2006 a fevereiro de 2008, procedentes de 25 unidades frigoríficas de cinco empresas habilitadas para comércio internacional localizadas em alguns Estados. Foram utilizados os primers U1/LI1 para Listeria spp. e LM1/LM2 e LL5/LL6 para L. monocytogenes. Listeria spp. foi detectada em 19,72% do total analisado, com percentuais de 23% em salas de desossa, câmaras-frias e meiascarcaças, 15% em cortes cárneos e 10% em salas de matança. Nos matadouros frigoríficos foi identificada em 19,42% no A, 17,72% no B, 22,95% no C e 20% no D e E. Em relação aos Estados, 16,34% em São Paulo, 23,94% no Mato Grosso do Sul, 14,06% em Goiás, 32% no Mato Grosso, 27,27% na Bahia e 14,28% no Pará foram positivas para o gênero. Independente do par de primers utilizado para amplificação, L. monocytogenes foi detectada em 14,72% da amostragem, sendo 20% em câmaras-frias, 15,83% em salas de desossa, 13,33% em meiascarcaças e em cortes cárneos e 10% em salas de matança. Foram obtidas 12,57% de amostras positivas no matadouro-frigorifico A, 11,39% no B, 21,31% no C e 13,33% no D e E. A espécie foi detectada em São Paulo, com 12,42% de amostras positivas, 14,08% no Mato Grosso do Sul, 12,30% em Goiás, 24% no Mato Grosso, 27,27% na Bahia e 14,28% no Pará. O par de primer LM1/LM2 revelou maior eficiência que o LL5/LL6 e, apesar deste último ter apresentado baixa eficiência para detecção, a totalidade de amostras positivas para L. monocytogenes deste trabalho só foi possível com a associação de ambos. Listeria spp. e L. monocytogenes se encontram amplamente distribuídas nas fontes e sítios estudados, estando disseminadas em todos os matadourosfrigoríficos e Estados analisados, com exceção de Minas Gerais. Os resultados deste trabalho revelam a necessidade de adoção de medidas urgentes para o controle do patógeno nos estabelecimentos de abate de bovinos no Brasil e devem servir de alerta para a possibilidade de ocorrência de surtos de listeriose de veiculação alimentar, dadas as freqüências de ocorrência aqui verificadas em meias-carcaças e cortes cárneosMade available in DSpace on 2014-07-29T15:07:51Z (GMT). No. of bitstreams: 1 Dissertacao2008_Leonado_Franca.pdf: 610450 bytes, checksum: 1fc7bfc6096780687d2226f632e6aa85 (MD5) Previous issue date: 2008-08-28application/pdfhttp://repositorio.bc.ufg.br/TEDE/retrieve/3714/Dissertacao2008_Leonado_Franca.pdf.jpgporUniversidade Federal de GoiásMestrado em Ciência AnimalUFGBRCiências Agrárias - Veterinariacarcaças, carne bovina fresca, cortes cárneos, saúde pública, PCR, segurança alimentarCarcass, fresh bovine meet, beef cuts, PCR, public health, food safetyCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOSListeria spp. e L. monocytogenes em carne bovina refrigerada e embalada a vácuo, equipamentos e ambiente de matadouros frigoríficosListeria spp. and L. monocytogenes in vacuum-packed chilled beef, equipment, environment of slaughterhousesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALDissertacao2008_Leonado_Franca.pdfapplication/pdf610450http://repositorio.bc.ufg.br/tede/bitstreams/9408a290-f75a-4202-baf1-2ebcadfdc049/download1fc7bfc6096780687d2226f632e6aa85MD51THUMBNAILDissertacao2008_Leonado_Franca.pdf.jpgDissertacao2008_Leonado_Franca.pdf.jpgGenerated Thumbnailimage/jpeg2104http://repositorio.bc.ufg.br/tede/bitstreams/6aa0c9ba-3ccd-41d0-bf2c-fe3b0194ac89/downloadc4715912a635b5fbde63d2a9b070733fMD52tde/9282014-07-30 03:07:33.741open.accessoai:repositorio.bc.ufg.br:tde/928http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2014-07-30T06:07:33Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)false |
dc.title.por.fl_str_mv |
Listeria spp. e L. monocytogenes em carne bovina refrigerada e embalada a vácuo, equipamentos e ambiente de matadouros frigoríficos |
dc.title.alternative.eng.fl_str_mv |
Listeria spp. and L. monocytogenes in vacuum-packed chilled beef, equipment, environment of slaughterhouses |
title |
Listeria spp. e L. monocytogenes em carne bovina refrigerada e embalada a vácuo, equipamentos e ambiente de matadouros frigoríficos |
spellingShingle |
Listeria spp. e L. monocytogenes em carne bovina refrigerada e embalada a vácuo, equipamentos e ambiente de matadouros frigoríficos FRANÇA, Leonardo carcaças, carne bovina fresca, cortes cárneos, saúde pública, PCR, segurança alimentar Carcass, fresh bovine meet, beef cuts, PCR, public health, food safety CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS |
title_short |
Listeria spp. e L. monocytogenes em carne bovina refrigerada e embalada a vácuo, equipamentos e ambiente de matadouros frigoríficos |
title_full |
Listeria spp. e L. monocytogenes em carne bovina refrigerada e embalada a vácuo, equipamentos e ambiente de matadouros frigoríficos |
title_fullStr |
Listeria spp. e L. monocytogenes em carne bovina refrigerada e embalada a vácuo, equipamentos e ambiente de matadouros frigoríficos |
title_full_unstemmed |
Listeria spp. e L. monocytogenes em carne bovina refrigerada e embalada a vácuo, equipamentos e ambiente de matadouros frigoríficos |
title_sort |
Listeria spp. e L. monocytogenes em carne bovina refrigerada e embalada a vácuo, equipamentos e ambiente de matadouros frigoríficos |
author |
FRANÇA, Leonardo |
author_facet |
FRANÇA, Leonardo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
NUNES, Iolanda Aparecida |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2555738917312914 |
dc.contributor.advisor-co1.fl_str_mv |
MESQUITA, Albenones José de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/3180029815183858 |
dc.contributor.advisor-co2.fl_str_mv |
MEIRELES, Marcelo Vasconcelos |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/0903513897615274 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0501694555123594 |
dc.contributor.author.fl_str_mv |
FRANÇA, Leonardo |
contributor_str_mv |
NUNES, Iolanda Aparecida MESQUITA, Albenones José de MEIRELES, Marcelo Vasconcelos |
dc.subject.por.fl_str_mv |
carcaças, carne bovina fresca, cortes cárneos, saúde pública, PCR, segurança alimentar |
topic |
carcaças, carne bovina fresca, cortes cárneos, saúde pública, PCR, segurança alimentar Carcass, fresh bovine meet, beef cuts, PCR, public health, food safety CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS |
dc.subject.eng.fl_str_mv |
Carcass, fresh bovine meet, beef cuts, PCR, public health, food safety |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS |
description |
Intending to stipulate the parcel of Listeria spp. and L. monocytogenes in several sources, using the PCR techicic, 360 samples were analyzed in the period between november 2006 and february 2008, proceeding from 25 commercial abattoirs of five companies all licensed to export from some Brazilian States. Primers U1/LI1 for Listeria spp. and LM1/LM2 and LL5/LL6 for L. monocytogenes. Listeria spp. was detected in 19,72% of the samples, with percentage of 23% in the bone rooms, cold chamber and half carcass, 15% in beef cuts and 10% in the slaughter rooms. In the slaughterhouses it was detected in 19,42% in the A, 17,72% in the B, 22,95% in the C and 20% in the D and E. Analyzing the states, 16,34% in São Paulo, 23,94% in Mato Grosso do Sul, 14,06% in Goiás, 32% in Mato Grosso, 27,27% in Bahia and 14,28% in Pará were positive for its genus. No matter the pair of ‟primers‟‟ used for amplication, L. monocytogenes were detected in 14,72% of the samples, 20% in cold chambers, 15,83% in bone rooms, 13.33% in halfcarcass and in beef cuts and 10% in slaughter rooms. In the slaughterhouse A, 12,57% of the samples were detected positive‟, 11,39% in B, 21,31% in C and 13,33% in D e E. The bacterium was detected in São Paulo in 12,42% of positive samples, 14,08% in Mato Grosso do Sul, 12,30% in Goiás, 24%, in Mato Grosso, 27,27% in Bahia and 14,28% in Pará. The pair of primer LM1/LM2 revealed better efficience than the LL5/LL6 and although this last one has presented low efficiency to detection, the total of positive samples of L. monocytogenes of this research only was possible by the association of both. Listeria spp. and L. monocytogenes are largely propagated in the sources and studied places, and, therefore, disseminated in all slaughterhouses and analyzed States, excepting Minas Gerais. The results of this research revealed the necessariness of adoption of urgent providences about the control of the pathogen in the bovine slaughterhouses in Brazil and must alert the possibility of occurrence of outbreak of listeriosis of nutritious transmission, considering the occurrence and the frequency repetition here verified in half-carcass and beef cuts |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-08-28 |
dc.date.available.fl_str_mv |
2010-02-04 |
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2014-07-29T15:07:51Z |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
FRANÇA, Leonardo. Listeria spp. and L. monocytogenes in vacuum-packed chilled beef, equipment, environment of slaughterhouses. 2008. 93 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2008. |
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http://repositorio.bc.ufg.br/tede/handle/tde/928 |
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ark:/38995/001300000cr1m |
identifier_str_mv |
FRANÇA, Leonardo. Listeria spp. and L. monocytogenes in vacuum-packed chilled beef, equipment, environment of slaughterhouses. 2008. 93 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2008. ark:/38995/001300000cr1m |
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http://repositorio.bc.ufg.br/tede/handle/tde/928 |
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Universidade Federal de Goiás |
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Mestrado em Ciência Animal |
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UFG |
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BR |
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Ciências Agrárias - Veterinaria |
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Universidade Federal de Goiás |
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