Isolamento e caracterização fenotípica e genotípica de Salmonella sp. em vísceras de frangos

• Autor(a) principal: Costa, Camila Silva de Carvalho
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/00130000068m6
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/8824
• topic: Antimicrobianos; Frangos; Genes de virulência; Genes de resistência; Vísceras; Antibiotics; Broiler; Virulence; Gene; Resistance genes; Viscera; CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS

Salmonella sp. in food of poultry origin represents a major problem for world public health. The viscera of broilers can also be contaminated with the microorganism being the potential carrier of this pathogen. In this context, the objective of this work was to investigate the occurrence of Salmonella sp. in edible and inedible viscera of broilers, the presence of virulence genes (invA, and spvC) and resistance genes (sul1 and bla TEM ), as well as susceptibility profile to antimicrobial bases. The samples were collected in two slaughterhouses where 405 samples were obtained, 150 hearts, 150 livers and 105 biliary vesicles. Of these, seven samples (1.73%) were positive. The serovars found were Salmonella Minnesota and Salmonella Swarzengrund. Antimicrobial principles were tested for susceptibility to antimicrobials. The highest percentages of resistance were observed for gentamicin (100%), ciprofloxacin (100%), enrofloxacin (100%) and florfenicol (100%), followed by ampicillin, doxycycline, tetracycline (71%) and amoxicillin (57%). All isolates were sensitive to sulfamethoxazole + trimethoprim. The invA gene was identified in all isolates. The The spvC gene was identified in 50% S. Minnesota. Regarding the resistance genes, bla TEM was identified in all isolates. The sul1 gene was detected in four of the seven isolates. From the results, S. Minnesota and S. Swarzengrund of avian origin have genes that enable invasion, as well as phenotypically express resistance to nine antimicrobial bases tested.