Microssistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicos
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/6201 |
Resumo: | This work describes several instrumental improvements to electrophoretic microchips (MSE) and microfluidic paper-based analytical devices (μPADs). The improvements are showed in terms of fabrication process, modification steps, control of sample volume and detection system. First off, a CO2 laser engraver was proposed to produce microchannels on a polymethyl methacrylate (PMMA) surface. All parameters were previously optimized in order to obtain a channel with the smallest dimension. Channels with 80 μm of deep and width were fabricated. MSE was coupled with amperometric detection and this work showed for the use of a decoupler produced by a mixture between PDMS and sugar the first time. The proof-of-concept testing of the final device demonstrated the analysis of a model mixture of phenolic compounds within 200 s with baseline resolution. Injection and control of the solution inside the microchannel was the another parameter improved during the development of this project. Hydrodynamic injector was developed using an electronic micropipette. The dispenser function of the micropipette can be explored to inject fractioned sample volumes inside the microchannel. The volume equaling 0.6 μL was considered ideal to completely fill the injection channel. In order to have better control of the sample volume, microdevices with different configuration were proposed. A microdevice with two auxiliary channels (split configuration) was select to realize the experimental part. When compared to electrokinetic injection, hydrodynamic showed better analytical performance including correcting the bias effect, which is the main problem related to electrokinetic injection. μPAD surface was modified by two different methodologies, using silica nanoparticles and chitosan. The modification process was used to solve the drawback related to the non-homogeneity or uniformity of color development in the detection zone. Silica nanoparticles and chitosan were incorporated on the cellulose surface to function as a new support to immobilize the selected enzyme. The modification process with both the nano and bio compounds improved the analytical performance, increased the reliability of the low cost platform and decreased the limit of detection (LD) of colorimetric system. The resulting LD for the glucose and uric acid assays were 23 and 37 μM, respectively. The enhanced analytical performance of modified the μPADs ensured for the first time the colorimetric detection of glucose in tear samples from four non-diabetic patients. The found concentration levels ranged from 130 to 380 μM. |
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Coltro, Wendell Karlos Tomazellihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4730248E9Coltro, Wendell Karlos TomazelliJesus, Dosil Pereira deSilva, Claudinei Alves daAntoniosi Filho, Nelson RobertoIonashiro, Elias Yukihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4211289E6Gabriel, Ellen Flávia Moreira2016-09-15T12:23:41Z2016-06-10GABRIEL, E. F. M. Microssistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicos. 2016.198 f. Tese (Doutorado em Química) - Universidade Federal de Goiás,Goiânia, 2016.http://repositorio.bc.ufg.br/tede/handle/tede/6201ark:/38995/0013000003jf4This work describes several instrumental improvements to electrophoretic microchips (MSE) and microfluidic paper-based analytical devices (μPADs). The improvements are showed in terms of fabrication process, modification steps, control of sample volume and detection system. First off, a CO2 laser engraver was proposed to produce microchannels on a polymethyl methacrylate (PMMA) surface. All parameters were previously optimized in order to obtain a channel with the smallest dimension. Channels with 80 μm of deep and width were fabricated. MSE was coupled with amperometric detection and this work showed for the use of a decoupler produced by a mixture between PDMS and sugar the first time. The proof-of-concept testing of the final device demonstrated the analysis of a model mixture of phenolic compounds within 200 s with baseline resolution. Injection and control of the solution inside the microchannel was the another parameter improved during the development of this project. Hydrodynamic injector was developed using an electronic micropipette. The dispenser function of the micropipette can be explored to inject fractioned sample volumes inside the microchannel. The volume equaling 0.6 μL was considered ideal to completely fill the injection channel. In order to have better control of the sample volume, microdevices with different configuration were proposed. A microdevice with two auxiliary channels (split configuration) was select to realize the experimental part. When compared to electrokinetic injection, hydrodynamic showed better analytical performance including correcting the bias effect, which is the main problem related to electrokinetic injection. μPAD surface was modified by two different methodologies, using silica nanoparticles and chitosan. The modification process was used to solve the drawback related to the non-homogeneity or uniformity of color development in the detection zone. Silica nanoparticles and chitosan were incorporated on the cellulose surface to function as a new support to immobilize the selected enzyme. The modification process with both the nano and bio compounds improved the analytical performance, increased the reliability of the low cost platform and decreased the limit of detection (LD) of colorimetric system. The resulting LD for the glucose and uric acid assays were 23 and 37 μM, respectively. The enhanced analytical performance of modified the μPADs ensured for the first time the colorimetric detection of glucose in tear samples from four non-diabetic patients. The found concentration levels ranged from 130 to 380 μM.O trabalho descrito nesta tese mostra uma variedade de melhorias instrumentais desenvolvida tanto para os microssistemas eletroforéticos (MSE) como para os dispositivos analíticos a base de papel (μPADs). As melhorias são discutidas em termos de processo de fabricação dos microdispositivos, modificação de superfície e manipulação da solução no interior dos microcanais. Inicialmente o uso de uma gravadora a laser foi proposta para a fabricação dos microcanais em substrato de poli(metilmetacrilato) (PMMA). Todos os parâmetros do laser foram otimizados afim de fabricar canais nas menores dimensões possíveis. Canais com aproximadamente 80 μm de largura e profundidade foram obtidas. Os MSE fabricados com laser foram acoplados ao sistema de detecção amperométrica em que foi proposto pela primeira vez o uso de um desacoplador alternativo fabricado a partir da mistura entre o poli(dimetisiloxano) (PDMS) e açúcar. A prova de conceito do dispositivo fabricado com a gravadora a laser associada a detecção amperométrica foi demostrada na separação de uma mistura de compostos fenólicos em menos de 200 s e com ótima resolução de linha de base. A etapa de injeção e manipulação de solução no interior dos microcanais foi outra melhoria instrumental proposta no desenvolvimento deste trabalho. Desenvolveu-se um injetor hidrodinâmico alternativo a partir do uso de uma micropipeta eletrônica comercial. A micropipeta possui uma função dispenser que pode ser explorado para injetar volumes fracionados no interior dos microcanais. O volume de 0,6 μL foi denominado como o ideal para realizar a etapa de injeção. No intuito de se ter um melhor controle do volume de amostra, um arranjo de microcanais foi proposto. Os dispositivos fabricados na configuração contendo dois canais auxiliares foram determinados como os ideais. Quando comparada a injeção eletrocinética, o injetor hidrodinâmico apresentou um ótimo desempenho analítico corrigindo principalmente o efeito bias, um dos principais problemas relacionados ao processo de injeção eletrocinética. Em relação aos μPADs, foram propostas duas diferentes estratégias de modificação de superfície afim de corrigir problemas correlacionados à falta de homogeneidade e uniformidade de cor gerada na zona de detecção. Nanopartículas de sílica e quitosana foram diretamente incorporadas na superfície da celulose servido como um novo suporte para imobilização enzimática. A modificação com o nano e biocomposto melhorou significativamente o desempenho analítico dos μPADs corrigindo o efeito de lavagem, aumentando a confiabilidade analítica e reduzindo os limites de detecção do sistema colorimétrico. Os limites de detecção (LD) encontrado para os ensaios de glicose e ácido úrico foram de 23 e 37 μM, respectivamente. Devido as melhoras analíticas encontrada foi possível pela primeira vez demonstrar o uso dos dispositivos a base de papel para na detecção de glicose em amostra de lágrimas. Foram medidos os níveis de glicose na lágrima de quatro pacientes não diabéticos. Os níveis de glicose encontrado variou entre 130 a 380 μM.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2016-09-12T17:12:09Z No. of bitstreams: 2 Tese - Ellen Flávia Moreira Gabriel - 2016.pdf: 5261570 bytes, checksum: 5ada90cb52637625bbd0a9853f342564 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-09-15T12:23:41Z (GMT) No. of bitstreams: 2 Tese - Ellen Flávia Moreira Gabriel - 2016.pdf: 5261570 bytes, checksum: 5ada90cb52637625bbd0a9853f342564 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2016-09-15T12:23:41Z (GMT). No. of bitstreams: 2 Tese - Ellen Flávia Moreira Gabriel - 2016.pdf: 5261570 bytes, checksum: 5ada90cb52637625bbd0a9853f342564 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-06-10Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Química (IQ)UFGBrasilInstituto de Química - IQ (RG)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMicrochips eletroforéticosMicrodispositivos de papelInjetor hidrodinâmicoEnsaios enzimáticosAnálises de lágrimaCIENCIAS EXATAS E DA TERRA::QUIMICAMicrossistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis66369392132541515860060060060078260667437411972781571700325303117195-2555911436985713659reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Microssistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicos |
title |
Microssistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicos |
spellingShingle |
Microssistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicos Gabriel, Ellen Flávia Moreira Microchips eletroforéticos Microdispositivos de papel Injetor hidrodinâmico Ensaios enzimáticos Análises de lágrima CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Microssistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicos |
title_full |
Microssistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicos |
title_fullStr |
Microssistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicos |
title_full_unstemmed |
Microssistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicos |
title_sort |
Microssistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicos |
author |
Gabriel, Ellen Flávia Moreira |
author_facet |
Gabriel, Ellen Flávia Moreira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Coltro, Wendell Karlos Tomazelli |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4730248E9 |
dc.contributor.referee1.fl_str_mv |
Coltro, Wendell Karlos Tomazelli |
dc.contributor.referee2.fl_str_mv |
Jesus, Dosil Pereira de |
dc.contributor.referee3.fl_str_mv |
Silva, Claudinei Alves da |
dc.contributor.referee4.fl_str_mv |
Antoniosi Filho, Nelson Roberto |
dc.contributor.referee5.fl_str_mv |
Ionashiro, Elias Yuki |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4211289E6 |
dc.contributor.author.fl_str_mv |
Gabriel, Ellen Flávia Moreira |
contributor_str_mv |
Coltro, Wendell Karlos Tomazelli Coltro, Wendell Karlos Tomazelli Jesus, Dosil Pereira de Silva, Claudinei Alves da Antoniosi Filho, Nelson Roberto Ionashiro, Elias Yuki |
dc.subject.por.fl_str_mv |
Microchips eletroforéticos Microdispositivos de papel Injetor hidrodinâmico Ensaios enzimáticos Análises de lágrima |
topic |
Microchips eletroforéticos Microdispositivos de papel Injetor hidrodinâmico Ensaios enzimáticos Análises de lágrima CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
This work describes several instrumental improvements to electrophoretic microchips (MSE) and microfluidic paper-based analytical devices (μPADs). The improvements are showed in terms of fabrication process, modification steps, control of sample volume and detection system. First off, a CO2 laser engraver was proposed to produce microchannels on a polymethyl methacrylate (PMMA) surface. All parameters were previously optimized in order to obtain a channel with the smallest dimension. Channels with 80 μm of deep and width were fabricated. MSE was coupled with amperometric detection and this work showed for the use of a decoupler produced by a mixture between PDMS and sugar the first time. The proof-of-concept testing of the final device demonstrated the analysis of a model mixture of phenolic compounds within 200 s with baseline resolution. Injection and control of the solution inside the microchannel was the another parameter improved during the development of this project. Hydrodynamic injector was developed using an electronic micropipette. The dispenser function of the micropipette can be explored to inject fractioned sample volumes inside the microchannel. The volume equaling 0.6 μL was considered ideal to completely fill the injection channel. In order to have better control of the sample volume, microdevices with different configuration were proposed. A microdevice with two auxiliary channels (split configuration) was select to realize the experimental part. When compared to electrokinetic injection, hydrodynamic showed better analytical performance including correcting the bias effect, which is the main problem related to electrokinetic injection. μPAD surface was modified by two different methodologies, using silica nanoparticles and chitosan. The modification process was used to solve the drawback related to the non-homogeneity or uniformity of color development in the detection zone. Silica nanoparticles and chitosan were incorporated on the cellulose surface to function as a new support to immobilize the selected enzyme. The modification process with both the nano and bio compounds improved the analytical performance, increased the reliability of the low cost platform and decreased the limit of detection (LD) of colorimetric system. The resulting LD for the glucose and uric acid assays were 23 and 37 μM, respectively. The enhanced analytical performance of modified the μPADs ensured for the first time the colorimetric detection of glucose in tear samples from four non-diabetic patients. The found concentration levels ranged from 130 to 380 μM. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-09-15T12:23:41Z |
dc.date.issued.fl_str_mv |
2016-06-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GABRIEL, E. F. M. Microssistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicos. 2016.198 f. Tese (Doutorado em Química) - Universidade Federal de Goiás,Goiânia, 2016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/6201 |
dc.identifier.dark.fl_str_mv |
ark:/38995/0013000003jf4 |
identifier_str_mv |
GABRIEL, E. F. M. Microssistemas eletroforéticos e dispositivos à base de papel: avanços instrumentais, incorporação de nanomateriais e diagnósticos clínicos. 2016.198 f. Tese (Doutorado em Química) - Universidade Federal de Goiás,Goiânia, 2016. ark:/38995/0013000003jf4 |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/6201 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
663693921325415158 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
7826066743741197278 |
dc.relation.cnpq.fl_str_mv |
1571700325303117195 |
dc.relation.sponsorship.fl_str_mv |
-2555911436985713659 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Química (IQ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Química - IQ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
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