Avaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanol
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/12788 |
Resumo: | The International Energy Agency estimates that ethanol from sugar cane reduces the emission of greenhouse gases by 89%. Brazil ranks second in world production of ethanol but still needs to improve in the coming years to reach the decarbonization targets proposed by the National Biofuel Policy. However, the process is consolidated the ethanol production improvement depends on the role performed by several factors as fermentation. Industrial yeasts are inoculated in the fermentation owing to their great fermentation characteristics but it may happen the entrance or appearance of contaminating microorganisms which are called wild yeasts. The process requires a wide range of minerals to reach adequate efficiency and a correct range of macro and micronutrients in the fermentation may improve the functioning of cellular metabolism and prevent the development of wild yeasts. This study aims to analyze the effects of mineral nutrients on ethanol production from synthetic sugarcane juice and a mixed culture of industrial and wild yeast. The industrial yeasts BG-1, CAT-1, FT-858L and PE-2 of the species Saccharomyces cerevisiae were used and the wild yeast was isolated from an Alcohol Power Plant in Goiás and called LFS. The yeasts were identified according to their macromorphological characteristics in the growth of colonies on plates. To assure the experimental reproducibility the synthetic sugarcane juice was used with 16% of sugars (m/v). The fermentation essays were evaluated by high performance liquid chromatography and residual sugars, glycerol, ethanol and acetic acid quantified. Minerals were quantified by atomic absorption spectrometry. The tests were carried out in the proportion of 30% of the wild yeast and 17.5% of each industrial yeast for 72 hours at 30ºC with 2.5 mL of the synthetic sugarcane juice. The inoculum was prepared from the scraping of the yeast present in the sterile plates and the solution mineral was added for the supplementation. The influence of Ca, Fe, Cu, K, P, N, S and Mg ions was evaluated at the end of fermentation through colony population dynamics, sugar residual and ethanol production. The tests showed that nitrogen, magnesium, sulfur and copper favor fermentation while calcium, phosphorus and iron are more prejudicial to the process. To evaluate the relationship between the prejudicial minerals was carried out a simplex-lattice mixture design with 3 components and results showed that excess calcium is prejudicial to ethanol production. Another simplexcentroid mixture design was carried out with the 4 elements identified as favorable in synthetic sugarcane juice with calcium excess and the contamination decreased by 67% with supplementation of nitrogen, magnesium and sulfur. Tests in natural sugarcane juice showed that reduction of contaminating was by 48% and confirmed that nitrogen, magnesium and sulfur are favorable to inhibit wild yeast growth. In conclusion, the tests demonstrated that supplementation of nitrogen, magnesium and sulfur reduces the contamination and increases ethanol production. As a suggestion, the addition of fertilizers with these minerals during the cultivation of sugarcane or in start fermentation may help to improve the industrial process. |
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Castiglioni, Gabriel Luishttp://lattes.cnpq.br/6050198962131737Freitas, Fernanda Ferreirahttp://lattes.cnpq.br/0883086742146577Castiglioni, Gabriel LuisVendruscolo, FrancieloRibeiro, Eloizio Júliohttp://lattes.cnpq.br/1570481013278036Silveira, Larissa Gabriele Silva2023-04-26T10:27:54Z2023-04-26T10:27:54Z2022-12-16SILVEIRA, L. G. S. Avaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanol. 2022. 98 f. Dissertação (Mestrado em Engenharia Química) - Universidade Federal de Goiás, Goiânia, 2022.http://repositorio.bc.ufg.br/tede/handle/tede/12788ark:/38995/0013000008gmtThe International Energy Agency estimates that ethanol from sugar cane reduces the emission of greenhouse gases by 89%. Brazil ranks second in world production of ethanol but still needs to improve in the coming years to reach the decarbonization targets proposed by the National Biofuel Policy. However, the process is consolidated the ethanol production improvement depends on the role performed by several factors as fermentation. Industrial yeasts are inoculated in the fermentation owing to their great fermentation characteristics but it may happen the entrance or appearance of contaminating microorganisms which are called wild yeasts. The process requires a wide range of minerals to reach adequate efficiency and a correct range of macro and micronutrients in the fermentation may improve the functioning of cellular metabolism and prevent the development of wild yeasts. This study aims to analyze the effects of mineral nutrients on ethanol production from synthetic sugarcane juice and a mixed culture of industrial and wild yeast. The industrial yeasts BG-1, CAT-1, FT-858L and PE-2 of the species Saccharomyces cerevisiae were used and the wild yeast was isolated from an Alcohol Power Plant in Goiás and called LFS. The yeasts were identified according to their macromorphological characteristics in the growth of colonies on plates. To assure the experimental reproducibility the synthetic sugarcane juice was used with 16% of sugars (m/v). The fermentation essays were evaluated by high performance liquid chromatography and residual sugars, glycerol, ethanol and acetic acid quantified. Minerals were quantified by atomic absorption spectrometry. The tests were carried out in the proportion of 30% of the wild yeast and 17.5% of each industrial yeast for 72 hours at 30ºC with 2.5 mL of the synthetic sugarcane juice. The inoculum was prepared from the scraping of the yeast present in the sterile plates and the solution mineral was added for the supplementation. The influence of Ca, Fe, Cu, K, P, N, S and Mg ions was evaluated at the end of fermentation through colony population dynamics, sugar residual and ethanol production. The tests showed that nitrogen, magnesium, sulfur and copper favor fermentation while calcium, phosphorus and iron are more prejudicial to the process. To evaluate the relationship between the prejudicial minerals was carried out a simplex-lattice mixture design with 3 components and results showed that excess calcium is prejudicial to ethanol production. Another simplexcentroid mixture design was carried out with the 4 elements identified as favorable in synthetic sugarcane juice with calcium excess and the contamination decreased by 67% with supplementation of nitrogen, magnesium and sulfur. Tests in natural sugarcane juice showed that reduction of contaminating was by 48% and confirmed that nitrogen, magnesium and sulfur are favorable to inhibit wild yeast growth. In conclusion, the tests demonstrated that supplementation of nitrogen, magnesium and sulfur reduces the contamination and increases ethanol production. As a suggestion, the addition of fertilizers with these minerals during the cultivation of sugarcane or in start fermentation may help to improve the industrial process.A Agência Internacional de Energia estima que o etanol proveniente da cana-de-açúcar reduz em 89% a emissão de gases de efeito estufa. O Brasil, mesmo ocupando o segundo lugar na geração global de etanol, ainda precisa crescer nos próximos anos para atingir as metas de descarbonização propostas pela Política Nacional de Biocombustíveis. Por mais que o processo de produção esteja consolidado, sua eficiência e melhoria dependem do papel desempenhado por diversos fatores, entre eles a fermentação. As cepas industriais são inoculadas no meio devido suas boas características fermentativas, entretanto, pode ocorrer a entrada ou surgimento de microrganismos contaminantes, que são chamados de leveduras selvagens. O processo requer uma vasta gama de minerais para atingir o rendimento adequado por isso, níveis adequados de macro e micronutrientes no meio permitem o bom funcionamento do metabolismo celular, a manutenção da viabilidade fermentativa e impedem o desenvolvimento das leveduras selvagens. Em vista disso, este estudo propõe a análise dos efeitos dos nutrientes minerais na fermentação dos açúcares contidos no caldo de cana-de-açúcar com cultura mista de leveduras industriais e selvagem. As leveduras industriais da espécie Saccharomyces cerevisiae BG-1, CAT-1, FT-858L e PE-2, e a levedura floculante selvagem isolada do processo produtivo de uma usina de etanol do estado de Goiás, chamada LFS, foram identificadas conforme suas características macromorfológicas no crescimento das colônias em placas. Para garantir a reprodutibilidade experimental, o mosto sintético com 16% de açúcares (m/v) foi usado, e sua caracterização por cromatografia líquida de alta performance identificou os açúcares residuais, glicerol, etanol e ácido acético, usados como parâmetros fermentativos. O teor mineral foi quantificado pela espectrometria de absorção atômica. Os ensaios foram conduzidos com 30% da levedura selvagem e 17,5% de cada levedura industrial, durante 72 horas, com 2,5 mL do mosto sintético, o inóculo preparado a partir da raspagem das cepas presentes nas placas de manutenção e a solução mineral. A influência dos íons Ca, Fe, Cu, K, P, N, S e Mg foi avaliada ao final da fermentação com base na dinâmica populacional da colônia, no consumo de açúcares e na produção de etanol. Os ensaios mostraram que nitrogênio, magnésio, enxofre e cobre favorecem a fermentação, enquanto cálcio, fósforo e ferro são mais prejudiciais ao processo. Para avaliar a relação entre os minerais danosos fez-se um delineamento de mistura simplex-láttice com 3 componentes e observouse que o cálcio isoladamente é o elemento mais prejudicial ao meio. Outro delineamento de mistura do tipo simplexcentróide foi realizado com os 4 elementos identificados como favoráveis em meios suplementados com cálcio e observou-se uma redução de 67% da contaminação inicial gerada pela interação entre N, Mg e S. Experimentos com mosto natural também foram realizados e comprovaram a redução em 48% ocasionada pela suplementação de nitrogênio, magnésio e enxofre. Assim, observa-se melhor eficiência fermentativa a partir da suplementação mineral de nitrogênio, magnésio e enxofre, com redução dos níveis de contaminação e aumento do rendimento de etanol, e sugere-se o a adição destes minerais em etapa industrial ou prematuramente no cultivo da cana a partir do uso de fertilizantes.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2023-04-25T10:47:35Z No. of bitstreams: 2 Dissertação - Larissa Gabriele Silva Silveira - 2022.pdf: 2626446 bytes, checksum: 641ac0394a7f9ca9e840de268f80f946 (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2023-04-26T10:27:54Z (GMT) No. of bitstreams: 2 Dissertação - Larissa Gabriele Silva Silveira - 2022.pdf: 2626446 bytes, checksum: 641ac0394a7f9ca9e840de268f80f946 (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Made available in DSpace on 2023-04-26T10:27:54Z (GMT). No. of bitstreams: 2 Dissertação - Larissa Gabriele Silva Silveira - 2022.pdf: 2626446 bytes, checksum: 641ac0394a7f9ca9e840de268f80f946 (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Previous issue date: 2022-12-16Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Engenharia Química (IQ)UFGBrasilInstituto de Química - IQ (RMG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessProdução de etanolFermentação industrialLeveduras selvagensNutrientes mineraisEthanol productionIndustrial fermentationWild yeastsMineral nutrientsENGENHARIAS::ENGENHARIA QUIMICA::PROCESSOS INDUSTRIAIS DE ENGENHARIA QUIMICAAvaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanolEvaluation of the mineral composition of sugarcane must in the fermentative performance with mixed culture of Saccharomyces cerevisiae yeasts diagnosis and solution of problems related to the industrial production of ethanolinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis51500500500500294621reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/107b4221-0339-4e24-85ce-521071b2ed3b/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/37cac8ee-4490-4e51-a3d0-05ec07374f21/download4460e5956bc1d1639be9ae6146a50347MD52ORIGINALDissertação - Larissa Gabriele Silva Silveira - 2022.pdfDissertação - Larissa Gabriele Silva Silveira - 2022.pdfapplication/pdf2626446http://repositorio.bc.ufg.br/tede/bitstreams/f57205ce-21b0-42bb-91d7-aa827fe7eed5/download641ac0394a7f9ca9e840de268f80f946MD53tede/127882023-04-26 07:27:54.673http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/12788http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2023-04-26T10:27:54Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.pt_BR.fl_str_mv |
Avaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanol |
dc.title.alternative.eng.fl_str_mv |
Evaluation of the mineral composition of sugarcane must in the fermentative performance with mixed culture of Saccharomyces cerevisiae yeasts diagnosis and solution of problems related to the industrial production of ethanol |
title |
Avaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanol |
spellingShingle |
Avaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanol Silveira, Larissa Gabriele Silva Produção de etanol Fermentação industrial Leveduras selvagens Nutrientes minerais Ethanol production Industrial fermentation Wild yeasts Mineral nutrients ENGENHARIAS::ENGENHARIA QUIMICA::PROCESSOS INDUSTRIAIS DE ENGENHARIA QUIMICA |
title_short |
Avaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanol |
title_full |
Avaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanol |
title_fullStr |
Avaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanol |
title_full_unstemmed |
Avaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanol |
title_sort |
Avaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanol |
author |
Silveira, Larissa Gabriele Silva |
author_facet |
Silveira, Larissa Gabriele Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Castiglioni, Gabriel Luis |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6050198962131737 |
dc.contributor.advisor-co1.fl_str_mv |
Freitas, Fernanda Ferreira |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0883086742146577 |
dc.contributor.referee1.fl_str_mv |
Castiglioni, Gabriel Luis |
dc.contributor.referee2.fl_str_mv |
Vendruscolo, Francielo |
dc.contributor.referee3.fl_str_mv |
Ribeiro, Eloizio Júlio |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1570481013278036 |
dc.contributor.author.fl_str_mv |
Silveira, Larissa Gabriele Silva |
contributor_str_mv |
Castiglioni, Gabriel Luis Freitas, Fernanda Ferreira Castiglioni, Gabriel Luis Vendruscolo, Francielo Ribeiro, Eloizio Júlio |
dc.subject.por.fl_str_mv |
Produção de etanol Fermentação industrial Leveduras selvagens Nutrientes minerais |
topic |
Produção de etanol Fermentação industrial Leveduras selvagens Nutrientes minerais Ethanol production Industrial fermentation Wild yeasts Mineral nutrients ENGENHARIAS::ENGENHARIA QUIMICA::PROCESSOS INDUSTRIAIS DE ENGENHARIA QUIMICA |
dc.subject.eng.fl_str_mv |
Ethanol production Industrial fermentation Wild yeasts Mineral nutrients |
dc.subject.cnpq.fl_str_mv |
ENGENHARIAS::ENGENHARIA QUIMICA::PROCESSOS INDUSTRIAIS DE ENGENHARIA QUIMICA |
description |
The International Energy Agency estimates that ethanol from sugar cane reduces the emission of greenhouse gases by 89%. Brazil ranks second in world production of ethanol but still needs to improve in the coming years to reach the decarbonization targets proposed by the National Biofuel Policy. However, the process is consolidated the ethanol production improvement depends on the role performed by several factors as fermentation. Industrial yeasts are inoculated in the fermentation owing to their great fermentation characteristics but it may happen the entrance or appearance of contaminating microorganisms which are called wild yeasts. The process requires a wide range of minerals to reach adequate efficiency and a correct range of macro and micronutrients in the fermentation may improve the functioning of cellular metabolism and prevent the development of wild yeasts. This study aims to analyze the effects of mineral nutrients on ethanol production from synthetic sugarcane juice and a mixed culture of industrial and wild yeast. The industrial yeasts BG-1, CAT-1, FT-858L and PE-2 of the species Saccharomyces cerevisiae were used and the wild yeast was isolated from an Alcohol Power Plant in Goiás and called LFS. The yeasts were identified according to their macromorphological characteristics in the growth of colonies on plates. To assure the experimental reproducibility the synthetic sugarcane juice was used with 16% of sugars (m/v). The fermentation essays were evaluated by high performance liquid chromatography and residual sugars, glycerol, ethanol and acetic acid quantified. Minerals were quantified by atomic absorption spectrometry. The tests were carried out in the proportion of 30% of the wild yeast and 17.5% of each industrial yeast for 72 hours at 30ºC with 2.5 mL of the synthetic sugarcane juice. The inoculum was prepared from the scraping of the yeast present in the sterile plates and the solution mineral was added for the supplementation. The influence of Ca, Fe, Cu, K, P, N, S and Mg ions was evaluated at the end of fermentation through colony population dynamics, sugar residual and ethanol production. The tests showed that nitrogen, magnesium, sulfur and copper favor fermentation while calcium, phosphorus and iron are more prejudicial to the process. To evaluate the relationship between the prejudicial minerals was carried out a simplex-lattice mixture design with 3 components and results showed that excess calcium is prejudicial to ethanol production. Another simplexcentroid mixture design was carried out with the 4 elements identified as favorable in synthetic sugarcane juice with calcium excess and the contamination decreased by 67% with supplementation of nitrogen, magnesium and sulfur. Tests in natural sugarcane juice showed that reduction of contaminating was by 48% and confirmed that nitrogen, magnesium and sulfur are favorable to inhibit wild yeast growth. In conclusion, the tests demonstrated that supplementation of nitrogen, magnesium and sulfur reduces the contamination and increases ethanol production. As a suggestion, the addition of fertilizers with these minerals during the cultivation of sugarcane or in start fermentation may help to improve the industrial process. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-12-16 |
dc.date.accessioned.fl_str_mv |
2023-04-26T10:27:54Z |
dc.date.available.fl_str_mv |
2023-04-26T10:27:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVEIRA, L. G. S. Avaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanol. 2022. 98 f. Dissertação (Mestrado em Engenharia Química) - Universidade Federal de Goiás, Goiânia, 2022. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/12788 |
dc.identifier.dark.fl_str_mv |
ark:/38995/0013000008gmt |
identifier_str_mv |
SILVEIRA, L. G. S. Avaliação da composição mineral do mosto de cana de açúcar no desempenho fermentativo com cultura mista de leveduras Saccharomyces cerevisiae diagnóstico e solução de problemas relacionados a produção industrial de etanol. 2022. 98 f. Dissertação (Mestrado em Engenharia Química) - Universidade Federal de Goiás, Goiânia, 2022. ark:/38995/0013000008gmt |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/12788 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
51 |
dc.relation.confidence.fl_str_mv |
500 500 500 500 |
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UFG |
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