Análise da diversidade genética e mutações no gene da integrase de isolados do HIV-1 de pacientes atendidos no município de Jataí/Goiás

• Autor(a) principal: Paula, Marcella Silva de
• Data de Publicação: 2018
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/0013000006dbk
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/8470
• topic: HIV-1; Resistência; Diversidade e Integrase HIV-1; Resistance; Diversity and integrase; HIV-1; CLINICA MEDICA::DOENCAS INFECCIOSAS E PARASITARIAS

INI have been available in Brazil since 2009, when the first INI, Raltegravir, became available for therapy of rescue of HIV + patients in therapeutic failure. In the year 2017, a second INI was introduced into ART of patients newly diagnosed with HIV-1, Dolutegravir, which had a higher genetic barrier and a single daily dose, replaced Efavirenz in the first line of treatment. However, despite the efficiency of INI, the emergence of viral variants resistant to these drugs is inevitable. For this reason, it is necessary to monitor resistance mutations to INI, which may lead to therapeutic failure, aiming at optimizing the therapeutic regimen and controlling HIV infection. The objective of this study was to evaluate the occurrence of INI mutations and the resistance profile in HIV + / AIDS patients in the city of Jataí/Goiás. The complete IN gene was sequenced from samples from INI-naive patients. Resistance mutations were identified by the Stanford-HIV and IAS-USA database. Viral subtypes were identified by phylogenetic analysis. Among the 52 samples analyzed, no primary mutation was identified. Two accessory mutations (T97A / G163K) were identified and these induce a low level of INI resistance. In total, 152 polymorphisms were identified. The most prevalent subtype was subtype B. Therefore, these data demonstrate that the IN region is still highly conserved, encouraging the use of INI in HIV-1 therapy, and assist in the mapping of HIV-1 genetic diversity in the Southwest region of Goiás.