Avaliação das atividades genotóxica, antigenotóxica, angiogênica e potencial de cicatrização do látex da Synadenium umbellatum Pax

• Autor(a) principal: Reis, Paulo Roberto de Melo
• Data de Publicação: 2009
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/0013000007kz7
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/12541
• topic: Genotoxicidade; Angiogênese; Cicatrização; Mutagenic; Angiogenesis; Wound Repair; CIENCIAS BIOLOGICAS

Synadenium umbellatum Pax (1894) is a vegetable species from Euphorbiaceae‟s family. The species of this family produce latex. The latex extracted from S. umbellatum has been used by the Brazilian people as anti-tumoral, anti-inflammatory and wound healing agents. However, this latex presents toxic substances and proteolic enzymes. The aim of this work was to evaluate the possible genotoxic, antigenotoxic, cytotoxic, angiogenic and wound healing activities of S. umbellatum latex (SuL) in rats. The genotoxic and antigenotoxic activities were evaluated by mouse bone marrow micronucleus test. An alkylant agent mitomycin C (4 mg.kg-1 body weight) was used as a positive control. For all doses, micronucleated polychromatic erythrocytes (MNPCE) frequency was evaluated after 24 hours of treatment. To evaluate the antimutagenic activity, animals were treated with 10, 30, 50 and 100 mg.kg-1 and 4 mg. kg-1 of mitomycin C (MMC) simultaneously. The frequency of MNPCE was evaluated 24 hours after exposure. Cytotoxicity was evaluated by the polychromatic and normochromatic erythrocytes ratio (PCE/NCE). Angiogenic activity of the SuL was evaluated in chorioallantoic membrane (CAM) of fertilized chicken eggs. The concentrations of the SuL were of 10 and 20 mg/mL. Wound healing activity was evaluated in skin of rats. The skin fragment was removed with area 3 x 2,5 cm and submitted to topic application with 5 mL of SuL (concentration 10 mg/mL) and positive and negative controls. The results showed that the SuL was strongly mutagenic, cytotoxic, and antimutagenic, and a moderate activity of anticytotoxicity. In the evaluation of the angiogenesis, the SuL has promoted the increase of the proliferation of blood vessels in the chorioallantoic xvii membrane of fertilized chicken eggs and it also induced the increase of velocity of wound healing in rat skin lesion.