Identificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinal
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/9714 |
Resumo: | Trypanosomatids are parasites that cause different diseases affecting mainly low-income populations from countries in development or underdeveloped countries. These diseases are classified as neglected tropical diseases (NTDs). The few drugs available for the treatment of these parasitic diseases present several problems regarding low efficacy, resistance, and toxicity, making the need of new therapeutic options an urgent task. In this sense, the aim of this work was the identification of novel drug candidates against trypanosomatids through the application of integrated strategies in Medicinal Chemistry. To achieve this goal, three studies were performed combining different computer assisted drug design (CADD) approaches and experimental validation. In the first study, 39 virtual hits were identified by quantitative structure activity relationships (QSAR)-based virtual screening (VS) and selected for experimental evaluation. Among them, 13 compounds were active and selective in vitro against intracellular T. cruzi. Additionally, an in silico multi-parameter analysis for evaluation and comparison of some pharmacokinetic (ADMET) and physicochemical properties, with potency and selectivity, allowed the prioritization of the most promising compounds for further in vivo assays. In the second study, VS was performed for identification of novel potential inhibitors of pyruvate kinase (PK) from Leishmania spp., allowing the discovery of 14 novel potential inhibitors of this enzyme. These compounds were submitted to experimental evaluation against L. infantum amastigotes. In the third study, a dual-target VS was performed, allowing the identification of 15 potential inhibitors of both PK and sterol 14 α-demethylase (CYP51) of Leishmania spp., also predicted as active compounds against L. infantum amastigotes by QSAR models. Therefore, this work has demonstrated the potential of the integration of computational and experimental methods for the identification of active compounds against trypanosomatids. |
id |
UFG-2_879ebc6e36eec4ad65fb6550ab28b927 |
---|---|
oai_identifier_str |
oai:repositorio.bc.ufg.br:tede/9714 |
network_acronym_str |
UFG-2 |
network_name_str |
Repositório Institucional da UFG |
repository_id_str |
|
spelling |
Andrade, Carolina Hortahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4745602P1Braga, Rodolpho de Camposhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4731114E4Andrade, Carolina HortaCastro, Ana Maria deOliveira, Milton Adriano Pelli deLacerda, Elisângela de Paula SilveiraScotti, Marcus Tulliushttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4455043E5Melo Filho, Cleber Camilo de2019-06-18T15:33:58Z2018-04-02MELO FILHO, Cleber Camilo de. Identificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinal. 2018. 164 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás,Goiânia, 2018.http://repositorio.bc.ufg.br/tede/handle/tede/9714Trypanosomatids are parasites that cause different diseases affecting mainly low-income populations from countries in development or underdeveloped countries. These diseases are classified as neglected tropical diseases (NTDs). The few drugs available for the treatment of these parasitic diseases present several problems regarding low efficacy, resistance, and toxicity, making the need of new therapeutic options an urgent task. In this sense, the aim of this work was the identification of novel drug candidates against trypanosomatids through the application of integrated strategies in Medicinal Chemistry. To achieve this goal, three studies were performed combining different computer assisted drug design (CADD) approaches and experimental validation. In the first study, 39 virtual hits were identified by quantitative structure activity relationships (QSAR)-based virtual screening (VS) and selected for experimental evaluation. Among them, 13 compounds were active and selective in vitro against intracellular T. cruzi. Additionally, an in silico multi-parameter analysis for evaluation and comparison of some pharmacokinetic (ADMET) and physicochemical properties, with potency and selectivity, allowed the prioritization of the most promising compounds for further in vivo assays. In the second study, VS was performed for identification of novel potential inhibitors of pyruvate kinase (PK) from Leishmania spp., allowing the discovery of 14 novel potential inhibitors of this enzyme. These compounds were submitted to experimental evaluation against L. infantum amastigotes. In the third study, a dual-target VS was performed, allowing the identification of 15 potential inhibitors of both PK and sterol 14 α-demethylase (CYP51) of Leishmania spp., also predicted as active compounds against L. infantum amastigotes by QSAR models. Therefore, this work has demonstrated the potential of the integration of computational and experimental methods for the identification of active compounds against trypanosomatids.Tripanossomatídeos são parasitos causadores de diferentes doenças que afetam principalmente populações de baixa renda de países subdesenvolvidos e em desenvolvimento, sendo classificadas como doenças tropicais negligenciadas (DTNs). Os poucos fármacos disponíveis para o tratamento dessas parasitoses apresentam diversos problemas relacionados à baixa eficácia, resistência e toxicidade, tornando urgente a busca por novas opções terapêuticas. Nesse contexto, o objetivo deste trabalho foi identificar novos candidatos a fármacos contra tripanossomatídeos através da aplicação de estratégias integradas em Química Medicinal. Para atingir esse objetivo, foram desenvolvidos três estudos combinando diferentes abordagens do planejamento de fármacos auxiliado por computador (CADD) e validação experimental. No primeiro estudo, a partir de uma triagem virtual (VS) baseada em modelos de relações quantitativas entre estrutura e atividade (QSAR), foram identificados e selecionados 39 hits virtuais para avaliação experimental. Dentre estes, 13 compostos foram ativos e seletivos in vitro contra T. cruzi intracelular. Foi realizada uma análise multiparamétrica in silico para avaliar e comparar algumas propriedades farmacocinéticas (ADMET), físico-químicas, com a potência e seletividade, que nos permitiu priorizar os compostos mais promissores para futuros ensaios in vivo. No segundo estudo, foi realizada uma VS para identificação de novos potenciais inibidores da enzima piruvato quinase (PK) de Leishmania spp., que levou a identificação e seleção de 14 novos potenciais inibidores dessa enzima. Estes compostos foram enviados para avaliação experimental contra L. infantum amastigota. No terceiro estudo, foi realizada uma VS com dois alvos (dual-target), que permitiu a identificação e seleção de 15 potenciais inibidores de ambas as enzimas PK e 14 esterol desmetilase (CYP51) de Leishmania spp., também preditos, através de modelos de QSAR, como ativos contra forma amastigota de L. infantum. Portanto, este trabalho demonstrou o potencial da integração de métodos computacionais e experimentais para a identificação de compostos ativos contra tripanossomatídeos.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2019-06-13T17:45:54Z No. of bitstreams: 2 Tese - Cleber Camilo de Melo Filho - 2018.pdf: 11884661 bytes, checksum: 3ce1da0656eebd1d5a32af851b6d7f56 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2019-06-18T15:33:58Z (GMT) No. of bitstreams: 2 Tese - Cleber Camilo de Melo Filho - 2018.pdf: 11884661 bytes, checksum: 3ce1da0656eebd1d5a32af851b6d7f56 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-06-18T15:33:58Z (GMT). No. of bitstreams: 2 Tese - Cleber Camilo de Melo Filho - 2018.pdf: 11884661 bytes, checksum: 3ce1da0656eebd1d5a32af851b6d7f56 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-04-02Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessTriagem virtualDescoberta de fármacosTrypanosoma cruziLeishmaniaTratamentoVirtual screeningDrug discoveryTreatmentCIENCIAS BIOLOGICAS::PARASITOLOGIAIdentificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinalIdentification of novel bioactive compounds against trypanosomatids through integrated strategies in medicinal chemistryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis6085308344741430434600600600600-7769011444564556288-4544576747271574306-2555911436985713659reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://repositorio.bc.ufg.br/tede/bitstreams/844ea533-00b1-4075-98b3-22545a4875c3/downloadbd3efa91386c1718a7f26a329fdcb468MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.bc.ufg.br/tede/bitstreams/2cad86d5-5cb8-44b5-a946-66849fa66748/download4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://repositorio.bc.ufg.br/tede/bitstreams/5377e628-1847-4285-8ad9-98ead83ee15a/downloadd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://repositorio.bc.ufg.br/tede/bitstreams/eba9f408-6b3f-4fcf-a073-397c695c213f/downloadd41d8cd98f00b204e9800998ecf8427eMD54ORIGINALTese - Cleber Camilo de Melo Filho - 2018.pdfTese - Cleber Camilo de Melo Filho - 2018.pdfapplication/pdf11884661http://repositorio.bc.ufg.br/tede/bitstreams/51675b43-8a7b-4d79-b36b-0f58d2af5a51/download3ce1da0656eebd1d5a32af851b6d7f56MD55tede/97142019-06-18 12:33:58.708http://creativecommons.org/licenses/by-nc-nd/4.0/Acesso Abertoopen.accessoai:repositorio.bc.ufg.br:tede/9714http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2019-06-18T15:33:58Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.eng.fl_str_mv |
Identificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinal |
dc.title.alternative.eng.fl_str_mv |
Identification of novel bioactive compounds against trypanosomatids through integrated strategies in medicinal chemistry |
title |
Identificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinal |
spellingShingle |
Identificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinal Melo Filho, Cleber Camilo de Triagem virtual Descoberta de fármacos Trypanosoma cruzi Leishmania Tratamento Virtual screening Drug discovery Treatment CIENCIAS BIOLOGICAS::PARASITOLOGIA |
title_short |
Identificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinal |
title_full |
Identificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinal |
title_fullStr |
Identificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinal |
title_full_unstemmed |
Identificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinal |
title_sort |
Identificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinal |
author |
Melo Filho, Cleber Camilo de |
author_facet |
Melo Filho, Cleber Camilo de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Andrade, Carolina Horta |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4745602P1 |
dc.contributor.advisor-co1.fl_str_mv |
Braga, Rodolpho de Campos |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4731114E4 |
dc.contributor.referee1.fl_str_mv |
Andrade, Carolina Horta |
dc.contributor.referee2.fl_str_mv |
Castro, Ana Maria de |
dc.contributor.referee3.fl_str_mv |
Oliveira, Milton Adriano Pelli de |
dc.contributor.referee4.fl_str_mv |
Lacerda, Elisângela de Paula Silveira |
dc.contributor.referee5.fl_str_mv |
Scotti, Marcus Tullius |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4455043E5 |
dc.contributor.author.fl_str_mv |
Melo Filho, Cleber Camilo de |
contributor_str_mv |
Andrade, Carolina Horta Braga, Rodolpho de Campos Andrade, Carolina Horta Castro, Ana Maria de Oliveira, Milton Adriano Pelli de Lacerda, Elisângela de Paula Silveira Scotti, Marcus Tullius |
dc.subject.por.fl_str_mv |
Triagem virtual Descoberta de fármacos Trypanosoma cruzi Leishmania Tratamento |
topic |
Triagem virtual Descoberta de fármacos Trypanosoma cruzi Leishmania Tratamento Virtual screening Drug discovery Treatment CIENCIAS BIOLOGICAS::PARASITOLOGIA |
dc.subject.eng.fl_str_mv |
Virtual screening Drug discovery Treatment |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::PARASITOLOGIA |
description |
Trypanosomatids are parasites that cause different diseases affecting mainly low-income populations from countries in development or underdeveloped countries. These diseases are classified as neglected tropical diseases (NTDs). The few drugs available for the treatment of these parasitic diseases present several problems regarding low efficacy, resistance, and toxicity, making the need of new therapeutic options an urgent task. In this sense, the aim of this work was the identification of novel drug candidates against trypanosomatids through the application of integrated strategies in Medicinal Chemistry. To achieve this goal, three studies were performed combining different computer assisted drug design (CADD) approaches and experimental validation. In the first study, 39 virtual hits were identified by quantitative structure activity relationships (QSAR)-based virtual screening (VS) and selected for experimental evaluation. Among them, 13 compounds were active and selective in vitro against intracellular T. cruzi. Additionally, an in silico multi-parameter analysis for evaluation and comparison of some pharmacokinetic (ADMET) and physicochemical properties, with potency and selectivity, allowed the prioritization of the most promising compounds for further in vivo assays. In the second study, VS was performed for identification of novel potential inhibitors of pyruvate kinase (PK) from Leishmania spp., allowing the discovery of 14 novel potential inhibitors of this enzyme. These compounds were submitted to experimental evaluation against L. infantum amastigotes. In the third study, a dual-target VS was performed, allowing the identification of 15 potential inhibitors of both PK and sterol 14 α-demethylase (CYP51) of Leishmania spp., also predicted as active compounds against L. infantum amastigotes by QSAR models. Therefore, this work has demonstrated the potential of the integration of computational and experimental methods for the identification of active compounds against trypanosomatids. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-04-02 |
dc.date.accessioned.fl_str_mv |
2019-06-18T15:33:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MELO FILHO, Cleber Camilo de. Identificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinal. 2018. 164 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás,Goiânia, 2018. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/9714 |
identifier_str_mv |
MELO FILHO, Cleber Camilo de. Identificação de novos compostos bioativos contra tripanossomatídeos a partir de estratégias integradas em química medicinal. 2018. 164 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás,Goiânia, 2018. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/9714 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
6085308344741430434 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-7769011444564556288 |
dc.relation.cnpq.fl_str_mv |
-4544576747271574306 |
dc.relation.sponsorship.fl_str_mv |
-2555911436985713659 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/844ea533-00b1-4075-98b3-22545a4875c3/download http://repositorio.bc.ufg.br/tede/bitstreams/2cad86d5-5cb8-44b5-a946-66849fa66748/download http://repositorio.bc.ufg.br/tede/bitstreams/5377e628-1847-4285-8ad9-98ead83ee15a/download http://repositorio.bc.ufg.br/tede/bitstreams/eba9f408-6b3f-4fcf-a073-397c695c213f/download http://repositorio.bc.ufg.br/tede/bitstreams/51675b43-8a7b-4d79-b36b-0f58d2af5a51/download |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e 3ce1da0656eebd1d5a32af851b6d7f56 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1798044332170674176 |