Caracterização citogenética de bovinos da raça Nelore (Bos taurus indicus, Linnaeus 1758)

• Autor(a) principal: Amancio, Andréia Pires
• Data de Publicação: 2019
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/0013000003r95
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/10083
• topic: Cariótipo; Citogenética molecular; Alterações cromossômicas estruturais; Bos taurus indicus; Molecular cytogenetics; Strtuctural chromosomal alterations; Bos taurus indicus; Karyotype; GENETICA::GENETICA ANIMAL

The agricultural sector has been standing out in the Brazilian economy in recent decades for significant increase in productivity and its growing importance for maintaining the country's balance of trade. After the introduction of cattle in Brazil and their subsequent dispersal throughout the national territory, there was an intense process of adaptation of certain groups to specific environmental conditions of each place or region, which is responsible for the formation of several local Brazilian cattle breeds. These animals may present numerical and/or structural chromosomal alterations, which may result in a group of undesired characteristics to the producer that intends to expand his herd or even for the preservation of characteristics of a species. The aim of this study was to standardize the C, GTG and NOR banding for the bovine subspecies (Bos taurus indicus, Linnaeus 1758) and subsequently produce a pancentromeric probe by amplification and labeling the centromeric regions of autosomal chromosomes of cattle. Bovine blood samples were collected to detect metaphasic chromosomes using the cell culture method. Subsequently, cytogenetic banding, amplification, chromosomal probe labeling and FISH technique were performed. Through the banding it was possible the correct chromosomal pairing of these animals and with the use of the pancentromeric probe, the marking of the centromere regions of all autosomal chromosomes. These results may offer a new tool that meets the needs of genetic improvement of these animals.