Avaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológico
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/6373 |
Resumo: | In this work we investigated the magnetothermal efficacy of two nanocarriers, magnetic fluids (MF) and magnetoliposomes (ML), with potential for oncology therapy. Ferrofluids based on manganese ferrite nanoparticles, surface coated with citric acid, were synthesized. Magnetoliposomes, vesicles containing magnetic nanoparticles inside, were prepared by the extrusion method. X-ray diffraction corfimed the spinel structure, while transmission electron microscopy revealed spherical nanoparticles with a lognormal size distribution and mean diameter of (11,1 ± 3,5) nm. Magnetization curves, at quasi-static conditions revealed particles at the superparamagnetic regime. A reduction in the saturation magnetization in comparison with bulk samples were explained by a nonmagnetic shell with thickness of 1.1 nm. The concentration and particle size distribution of the liposomes (mean diameter of 180 nm) were obtained from the nanoparticle tracking analysis (NTA). The combination of magnetometry and NTA data revealed an encapsulation particle volume fraction in the range of 0.26 to 0.83% for the samples. Magnetic birefringence data showed a higher fraction of aggregates in ML in comparison to MF. Magnetic hyperthermia experiments were perfomed at distinct frequencies (111 -632 kHz) and field amplitudes (up to 250 Oe) for distinct ML and MF samples. In general, we found a reduction of magnetothermal efficacy for magnetoliposomes when compared to MF. although the effect is less evident at lower frequencies (<170 kHz). The explanation was obtained through a new theoretical model that, not only takes into account the effect of dipolar interaction in the magnetic anisotropy, but also the reduction of susceptibility due to blocked nanoparticles. This contribution was shown to be strongly dependent upon field frequency, amplitude and aggregates in the suspension. |
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Bakuzis, Andris Fisgueiroahttp://lattes.cnpq.br/3477269475651042Bakuzis, Andris FigueiroaMiotto, RoneiSilva, Sebastião William daBufaiçal, Leandro FelixPelegrini, Fernandohttp://lattes.cnpq.br/8747599940360430Silva, Anderson Costa da2016-10-10T15:51:04Z2016-08-17SILVA, Anderson Costa da. Avaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológico. 2015. 137 f. Tese (Doutorado em Física) - Universidade Federal de Goiás, Goiânia, 2016.http://repositorio.bc.ufg.br/tede/handle/tede/6373In this work we investigated the magnetothermal efficacy of two nanocarriers, magnetic fluids (MF) and magnetoliposomes (ML), with potential for oncology therapy. Ferrofluids based on manganese ferrite nanoparticles, surface coated with citric acid, were synthesized. Magnetoliposomes, vesicles containing magnetic nanoparticles inside, were prepared by the extrusion method. X-ray diffraction corfimed the spinel structure, while transmission electron microscopy revealed spherical nanoparticles with a lognormal size distribution and mean diameter of (11,1 ± 3,5) nm. Magnetization curves, at quasi-static conditions revealed particles at the superparamagnetic regime. A reduction in the saturation magnetization in comparison with bulk samples were explained by a nonmagnetic shell with thickness of 1.1 nm. The concentration and particle size distribution of the liposomes (mean diameter of 180 nm) were obtained from the nanoparticle tracking analysis (NTA). The combination of magnetometry and NTA data revealed an encapsulation particle volume fraction in the range of 0.26 to 0.83% for the samples. Magnetic birefringence data showed a higher fraction of aggregates in ML in comparison to MF. Magnetic hyperthermia experiments were perfomed at distinct frequencies (111 -632 kHz) and field amplitudes (up to 250 Oe) for distinct ML and MF samples. In general, we found a reduction of magnetothermal efficacy for magnetoliposomes when compared to MF. although the effect is less evident at lower frequencies (<170 kHz). The explanation was obtained through a new theoretical model that, not only takes into account the effect of dipolar interaction in the magnetic anisotropy, but also the reduction of susceptibility due to blocked nanoparticles. This contribution was shown to be strongly dependent upon field frequency, amplitude and aggregates in the suspension.Neste trabalho investigamos a eficiência magneto-térmica de dois nanocarreadores, fluidos magnéticos (FMs) e magnetolipossomos (MLs), com potencial para terapia oncológica. Fluidos magnéticos a base de nanopartículas de ferrita de manganês recobertas com ácido cítrico foram sintetizados. Magnetolipossomos, vesículas contendo as mesmas nanopartículas, foram preparados pelo método de extrusão. A difração de raios-X confirmou a estrutura cristalina das nanopartículas, do tipo espinélio, enquanto a microscopia eletrônica de transmissão indicou partículas esféricas com distribuição do tipo lognormal e diâmetro medio de (11,1 ± 3,5) nm. Os dados de magnetometria de amostra vibrante (MAV) revelaram partículas no regime superparamagnético em condição quasi-estática. A redução da magnetização de saturação da nanopartícula em relação a amostra bulk foi explicada pela existência de uma casca fracamente magnética com espessura de 1,1 nm. A concentração e distribuição de diâmetros dos lipossomos (diâmetro médio de 180 nm) foram obtidos pela técnica de análise de monitoramento de nanopartículas (NTA). Os dados de NTA e MAV permitiram obter a fração de partículas encapsuladas nas vesículas, que variou de 0,26 a 0,83%. Medidas de birrefringência magnética revelaram uma maior fração de partículas formando agregados nos MLs em relação aos FMs. Medidas de hipertermia magnética foram feitas variando a frequência (111 -632 kHz) e amplitude de campo magnético (até 250 Oe) em amostras de FMs e MLs com diferentes concentrações de partículas. Em geral, encontramos que a eficiência magnetotérmica dos MLs foi menor que a dos FMs, apesar deste efeito ser menos significativo em baixas frequencias (< 170 kHz). A explicação para o fenômeno foi fornecida por um novo modelo teórico que considerou, além do efeito da interação dipolar na anisotropia magnética, a redução da susceptibilidade devido a partículas bloqueadas. Esta contribuição foi fortemente dependente da frequência, amplitude do campo alternado e de agregados no colóide.Submitted by Erika Demachki (erikademachki@gmail.com) on 2016-10-07T17:34:38Z No. of bitstreams: 2 Tese - Anderson Costa da Silva - 2015.pdf: 4359148 bytes, checksum: 7b5f3865e16068f45cad2336c7692f09 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-10-10T15:51:04Z (GMT) No. of bitstreams: 2 Tese - Anderson Costa da Silva - 2015.pdf: 4359148 bytes, checksum: 7b5f3865e16068f45cad2336c7692f09 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2016-10-10T15:51:04Z (GMT). No. of bitstreams: 2 Tese - Anderson Costa da Silva - 2015.pdf: 4359148 bytes, checksum: 7b5f3865e16068f45cad2336c7692f09 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-08-17Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEGapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Fisica (IF)UFGBrasilInstituto de Física - IF (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMagnetolipossomosMagnetoliposomeCIENCIAS EXATAS E DA TERRA::FISICAAvaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológicoExperimental and theoretical evaluation of magnetoliposomes magnetic hyperthermia for oncological treatmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis3162138865744262028600600600600-4029658853652049306-8327146296503745929-961409807440757778reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Avaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológico |
dc.title.alternative.eng.fl_str_mv |
Experimental and theoretical evaluation of magnetoliposomes magnetic hyperthermia for oncological treatment |
title |
Avaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológico |
spellingShingle |
Avaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológico Silva, Anderson Costa da Magnetolipossomos Magnetoliposome CIENCIAS EXATAS E DA TERRA::FISICA |
title_short |
Avaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológico |
title_full |
Avaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológico |
title_fullStr |
Avaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológico |
title_full_unstemmed |
Avaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológico |
title_sort |
Avaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológico |
author |
Silva, Anderson Costa da |
author_facet |
Silva, Anderson Costa da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Bakuzis, Andris Fisgueiroa |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3477269475651042 |
dc.contributor.referee1.fl_str_mv |
Bakuzis, Andris Figueiroa |
dc.contributor.referee2.fl_str_mv |
Miotto, Ronei |
dc.contributor.referee3.fl_str_mv |
Silva, Sebastião William da |
dc.contributor.referee4.fl_str_mv |
Bufaiçal, Leandro Felix |
dc.contributor.referee5.fl_str_mv |
Pelegrini, Fernando |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8747599940360430 |
dc.contributor.author.fl_str_mv |
Silva, Anderson Costa da |
contributor_str_mv |
Bakuzis, Andris Fisgueiroa Bakuzis, Andris Figueiroa Miotto, Ronei Silva, Sebastião William da Bufaiçal, Leandro Felix Pelegrini, Fernando |
dc.subject.por.fl_str_mv |
Magnetolipossomos |
topic |
Magnetolipossomos Magnetoliposome CIENCIAS EXATAS E DA TERRA::FISICA |
dc.subject.eng.fl_str_mv |
Magnetoliposome |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::FISICA |
description |
In this work we investigated the magnetothermal efficacy of two nanocarriers, magnetic fluids (MF) and magnetoliposomes (ML), with potential for oncology therapy. Ferrofluids based on manganese ferrite nanoparticles, surface coated with citric acid, were synthesized. Magnetoliposomes, vesicles containing magnetic nanoparticles inside, were prepared by the extrusion method. X-ray diffraction corfimed the spinel structure, while transmission electron microscopy revealed spherical nanoparticles with a lognormal size distribution and mean diameter of (11,1 ± 3,5) nm. Magnetization curves, at quasi-static conditions revealed particles at the superparamagnetic regime. A reduction in the saturation magnetization in comparison with bulk samples were explained by a nonmagnetic shell with thickness of 1.1 nm. The concentration and particle size distribution of the liposomes (mean diameter of 180 nm) were obtained from the nanoparticle tracking analysis (NTA). The combination of magnetometry and NTA data revealed an encapsulation particle volume fraction in the range of 0.26 to 0.83% for the samples. Magnetic birefringence data showed a higher fraction of aggregates in ML in comparison to MF. Magnetic hyperthermia experiments were perfomed at distinct frequencies (111 -632 kHz) and field amplitudes (up to 250 Oe) for distinct ML and MF samples. In general, we found a reduction of magnetothermal efficacy for magnetoliposomes when compared to MF. although the effect is less evident at lower frequencies (<170 kHz). The explanation was obtained through a new theoretical model that, not only takes into account the effect of dipolar interaction in the magnetic anisotropy, but also the reduction of susceptibility due to blocked nanoparticles. This contribution was shown to be strongly dependent upon field frequency, amplitude and aggregates in the suspension. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-10-10T15:51:04Z |
dc.date.issued.fl_str_mv |
2016-08-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Anderson Costa da. Avaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológico. 2015. 137 f. Tese (Doutorado em Física) - Universidade Federal de Goiás, Goiânia, 2016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/6373 |
identifier_str_mv |
SILVA, Anderson Costa da. Avaliação experimental e teórica da hipertermia magnética de magnetolipossomos para tratamento oncológico. 2015. 137 f. Tese (Doutorado em Física) - Universidade Federal de Goiás, Goiânia, 2016. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/6373 |
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por |
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por |
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dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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UFG |
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Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Física - IF (RG) |
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Universidade Federal de Goiás |
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