Citotoxicidade de fios ortodônticos de níquel - titânio superelásticos e termoativado

• Autor(a) principal: Lopes, Rúbia Lorena Siqueira Garcia
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/9857
• topic: Citotoxicidade; Níquel; Fios ortodônticos; Cytotoxicity; Nickel; Orthodontic wires; ODONTOLOGIA::CLINICA ODONTOLOGICA

Introduction: the use of wires or any other orthodontic appliance that contains nickel can sensitize an individual not previously sensitive. Nickel titanium wires (NiTi) are used during many stages of orthodontic treatment, staying for a considerable time in contact with the mucous and saliva. Purpose: To assess "in vitro" fibroblast cell viability in relation to nickel present in cytotoxicity superelastic nickel-titanium wire and termoativado. Materials and Methods: The study "in vitro" used strains of murine fibroblasts commercially available in order to evaluate the cytotoxicity of NiTi wires 0014 '. L929 cells were cultured, exposed to extracts from immersion of the wires in artificial saliva for 30 to 60 days. The sample consisted of four commercial brands: Morelli, Orthometric, GAC and Abzil three types of alloys: stainless steel, superelastic NiTi and NiTi termoativado. All wires were sterilized in an autoclave, according to the manufacturer's instructions. Tests were performed to verify the release of nickel ions in the artificial saliva, atomic absorption spectrophotometry (AAS). The Scanning Electron Microscopy (SEM) to analyze the surface morphology. Elemental analysis of the surface of the wire by Energy Dispersive Spectroscopy (EDS). And cell viability testing, to test the cytotoxicity of nickel through the cell culture extracts. The two-way ANOVA test was used to evaluate the interaction of wire marks and alloys and the t- test was used to compare the times of 30 and 60 days with p <0.05. Results: In the EAA there was the release of nickel ions, with statistically significant differences between the brands and types of alloys in the time of 60 days. In SEM of the wires was not observed morphological differences between samples and their controls. There was no cytotoxicity when cultures were exposed to the sample extracts.