Avaliação da atividade hemaglutinante e antibacteriana dos extratos proteicos intracelulares e extracelulares de bactérias isoladas a partir de corais marinhos

• Autor(a) principal: Pereira, Thairyne Naruan Alves
• Data de Publicação: 2023
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/12847
• topic: Corais de viveiro; Bactérias marinhas; Potencial antibacteriano; Aquarius corals; Marine bacteria; Antibacterial potential; OUTROS

For decades, the terrestrial environment was the center of studies, however, research was extended from the terrestrial to the marine scope, in order to discover new molecules. Corals are marine animals that are present in seas, oceans and marine nurseries. These animals can live in symbiosis with several organisms, including bacteria that produce molecules of high biotechnological interest. From the collection, isolation and cultivation of these organisms, it is possible to obtain molecules of biotechnological interest. In this context, this study aims to isolate bacterial colonies from marine corals, evaluate the hemagglutinating activity and antibacterial activity of intracellular and extracellular protein extracts from bacterial colonies isolated from Green Star Polyps and Xenia Pompom corals, and obtain the protein profile of the extracts from SDS-PAGE electrophoresis. Eighteen colonies of bacteria were isolated, 9 colonies from each coral. Crude extracts were obtained from the cultivation of isolated bacteria in two different culture media, which were subsequently subjected to 60% saturation with ammonium sulfate. For the hemagglutinating activity, only the extracellular extracts showed activity, which allows inferring that they may contain proteins that have different biological activities, such as antibacterial and antiviral. Therefore, carbohydrate inhibition was performed, however, there was no inhibition of hemagglutinating activity for the following sugars tested, galactose, fructose, maltose, glucose, xylose, rhaminose and sucrose. Therefore, these extracellular extracts were subjected to antibacterial activity, which was determined by the Minimum Inhibitory Concentration (MIC) method, by the broth microdilution technique, against the microorganisms Escherichia coli, Staphylococcus aureus, Bacillus cereus, Salmonella typhimurium, and Enterococcus faecalis. There was antibacterial activity of three (E1 G13, E2 G13 and E2 P2) of the four protein extracts against Enterococcus faecalis. Protein extracts E1 G13, E2 G13 and E1 P2 showed inhibition of microbial growth against another strain tested, obtaining a concentration of 1000 µg.mL-1 for Escherichia coli. The four extracellular protein extracts obtained hemagglutination for the three blood types tested and are promising antibacterial agents, which can contribute to the expansion of natural products of marine origin.