Efeito antibacteriano dos sistemas Self-Ajusting File, XP-endo finisher e irrigação ultrassônica passiva sobre biofilme de Enterococcus faecalis

• Autor(a) principal: Sousa, Vinicius Caixeta de
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/0013000009jwh
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/7290
• topic: Preparo do canal radicular; Self-Adjusting File; Xp-Endo Finisher; Irrigação ultrassônica passiva; Ação antibacteriana; Root canal preparation; Self-Adjusting File; Xp-Endo Finisher; Passive ultrasonic irrigation; Antibacterial action; CIENCIAS DA SAUDE

Abstract: Objective: To evaluate the effectiveness of complementary protocols of sanitization in the decontamination of infected root canals. Methods: Fifty uniradicular maxilary anterior human teeth were selected. The crowns were removed and the lengths standardized at 16 mm. The specimens were inoculated with Enterococcus faecalis and incubated at 37 °C for sixty days. Thirty teeth were performed with BioRace instruments until diameter corresponding to #60.02, and then complemented with Self-Adjusting File (SAF); XP-endo Finisher (XPF) and passive ultrasonic irrigation (PUI). Ten samples were used as positive control and 10 were not contaminated. Initial and final samples were collected and incubated at 37 °C for a period of 48 hours. The bacterial growth was analyzed in culture, determining the presence or absence of bacteria. The optical density of the culture medium was interpreted by UV spectrophotometry. The specimens were sectioned and prepared for evaluation in SEM. The images of the root surfaces were analyzed and classified by scores according to the presence of debris. The Kruskal-Wallis test was used for statistical analysis. The level of significance was 5%. Results: The mean optical density (μm) of the sanification protocols showed bacterial reduction in all groups. The experimental groups did not present statistically significant differences (p = 0.196). The analysis of SEM images revealed no significant difference (p = 0.414) between the scores of the groups. Conclusion: Complementary sanitization protocols reduced bacterial contamination.