Análise proteômica de membrans de Paracoccidioides sp. durante privação de zinco

• Autor(a) principal: Silva, Marielle Garcia
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/0013000000d4j
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/7643
• topic: Proteoma; Proteínas de membranas; Privação de zinco; Proteome; Membranes proteins; Zinc deprivation; CIENCIAS BIOLOGICAS::MICROBIOLOGIA

Paracoccidioides spp. are pathogenic fungi that causes paracoccidioidomycosis, an important systemic mycosis in Latin American countries. The success of infection depends on the pathogen ability to obtain essential micronutrients (metals) from the host. This process of nutrient uptake occurs through membrane associated transporters. The membranes are constituted of a lipid bilayer with associated proteins and are involved in different processes during the establishment of infection, such as transport of nutrients and homeostatic regulation. As zinc is a metal that plays an important role in the regulation of host-pathogen interaction and changes in this micronutrient homeostasis are implicated in the pathogenesis of infectious diseases, the aim of this study was to identify the membrane proteins expressed under conditions of zinc deprivation. NanoUPLC-MSE technique was employed in order to identify membrane proteins of yeast cells (Pb01) grown in chemically defined media in the presence and absence of zinc. Transmission electron microscopy was performed to confirm the sample enrichment with membranes. Subsequently, the samples were digested and analyzed by NanoUPLC-MSE. In silico analysis was performed to determine the location of 460 proteins identified in extracts of Pb01 grown in + Zn (control) and TPEN (treated) medium. Among the identified proteins, 141 were classified as belonging to membranes and of those, 120 proteins were repressed and 21 were induced during zinc deprivation. Among the 141 membrane proteins, 81 showed transmembrane domains and 9 were classified as membranes proteins with post-transcriptional modification. A total of 15 membrane proteins showed signal peptide. Analysis of the function of membrane proteins, allowed the description that phospholipid metabolism and cell integrity maintenance pathways were affected by zinc deprivation.