Prevalência de tipos específicos de HPV e anormalidades citológicas em mulheres quilombolas

• Autor(a) principal: Batista, José Eduardo
• Data de Publicação: 2014
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/0013000002zhv
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/5955
• topic: Papilomavírus humano; Citologia; PCR; Diagnóstico; Prevalência; Human papillomavirus; Cytology; PCR; Diagnosis; Prevalence; CIENCIAS BIOLOGICAS::MICROBIOLOGIA

Introduction: Genital infection by human papillomavirus (HPV) is considered the most common sexually transmitted infection in several countries. The prevalence of HPV genotypes in women with or without cytological abnormalities may vary according to the studied population and the region. Objective: To detect and identify specific HPV types correlating with sociodemographic/behavioral characteristics and cytological abnormalities present in cervical smears of Maroon women. Methods: This cross-sectional study included 353 maroon women users of the unified health System of the municipalities of Maranhão for screening of cervical cancer. The samples were analyzed for the presence of cytological abnormalities by conventional methods and tested for 37 HPV genotypes by polymerase chain reaction (PCR) with primers PGMY09/11 followed by reverse line blot hybridization performed with the Linear Array HPV Genotyping Test kit by Roche Molecular System®. The association of HPV types and cytological diagnosis was investigated according to the different age groups. Results: HPV infection was detected in 13% (46/353) of the cases. Infection types with high-risk HPV were more frequent (78,3 %, 36/46) than for low-risk HPV (21,7 %, 10/46). Genotypes 68 (24,2 % - 11 /46), 58 (19,8% - 9/46), 52 and 31 (10,8% - 5/46 each), and 62 (8,8% 4/46) were the most prevalent. Only 6,6% (3/46) of the cases were positive for HPV 61. The genotypes 73, 70, 54, 53, 45, IS39 and 18, individually represented 4,4% (2/46) of all cases. The CP6 -108, 84, 72, 71, 66, 59, 56, 55, 51, 39, 33 and 16 genotypes individually represented 2,2% (1/46) of the cases. In women without cytological abnormalities, viral infections, both simple and multiple, were detected in 10,7%(35/328) of the cases. For those diagnosed with an abnormality, the prevalence of HPV infection was 24,0% (1 /46), being higher in high grade squamous intraepithelial lesion (HSIL-75,0 %), followed by atypical squamous cells cannot exclude high-grade squamous intraepithelial lesions (ASC-H, 50%), low grade squamous intraepithelial lesions (LSIL-33,4 %) and atypical squamous cells of undetermined significance (ASC-US, 14,3%). The analysis showed a statistically significant association between HPV infection and the detection of cytologic abnormalities in the age groups between 31-40 years (OR = 7.40, 95 % CI :1.07 -51 ,19 , p = 0.03) and 51 to 60 years (OR = 20.4 , 95 % CI : 1.12 to 704.69, p = 0.03). None of the behavioral variables showed significant association with HPV infection, however the presence of this infection was positively associated with detection of cytologic abnormalities (OR=6,57: IC:2,772-15,606), p=0,001. Conclusion: It is possible that the results of this study are due to characteristics of the study population, geographically isolated, with conservative habits and sexual intercourse only between members of the Quilombo. This study with Maroon women allowed us to delineate the epidemiology of this HPV infection in populations living in Maranhão.