Remoção de hormônios sexuais sintéticos por carbonização hidrotermal e por fungos de decomposição branca

• Autor(a) principal: Morais, Ruiter Lima
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/0013000002cg8
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/4271
• topic: Hormônios sexuais sintéticos; Carbonização hidrotermal; Fungos de decomposição branca; Synthetic sex hormones; Hydrothermal carbonization; White decomposition fungal; SANEAMENTO AMBIENTAL::QUALIDADE DO AR, DAS AGUAS E DO SOLO

Contaminations of water and wastewater with endocrine disrupters from domestic and industrial discharges have been proven in several regions of the planet. Among the endocrine disruptors, the synthetic sex hormones cause great concern because the presence of phenolic rings in their structures makes them stable and recalcitrant in the environment. In Goiás, the presence of synthetic sex hormone concentrations of ethinylestradiol in mg.L-1 in the Meia Ponte River, which goes accross the metropolitan area of Goiânia, was recently confirmed. Studies have shown that concentrations of ng.L-1 can affect sexual differentiation and cause serious damage to the reproductive system in fish and humans. Further, there are few techniques that are proven of being effective on removing this type of substance. The aim of this study was to evaluate the removal of synthetic sex hormones ethinylestradiol, gestodene, and cyproterone acetate by the hydrothermal carbonization process and biological treatment with fungi decomposing white Pycnoporus sanguineus and Trametes villosa. For this purpose, solutions were prepared, individually and in combination with a concentration of 1,0 μg.mL-1 and pH correction into the range of 2-3 with solutions of phosphoric acid or citric acid for treatment with hydrothermal carbonization. For the treatment with the fungus in liquid culture medium and under the condition of stirring, a solution was prepared containing all three hormones (ethinyl estradiol, cyproterone acetate and gestodene) in concentrations of 0,333 μg.mL-1. The carbonization treatments by the process of hydrothermal three hormones with pH correction made with phosphoric acid and reaction time of 90 minutes showed satisfactory removal of Ethinylestradiol and Gestodene, above 90%. Already in the individual treatments, removal achieved was higher than 99% for all three hormones. The biological treatment with Pycnoporus sanguineus and Trametes villosa show significant removal of hormones and cyproterone acetate Ethinylestradiol above 99% and 78.9% for Gestodene for both fungi. It was possible to notice that the presence of hormones increased the enzymatic production of laccase, with peak production anticipated for the fungus Pycnoporus sanguineus. The toxicity test with Artemia salina was observed that the solution with the three hormones after carbonization hydrothermal treatment had fewer dead larvae of Artemia salina, than the solution with hormones without treatment. In test germination of Allium cepa, the solution treated with hormones hydrothermal carbonization showed the same germination rate of the control group, however, less vigor of the shoots. As the control group, the solution to the three hormones before treatment and after biological treatment, the 9.09% concentration, showed no mortality of larvae of Artemia salina. These results show that both the hydrothermal carbonization process as with the fungus Pycnoporus sanguineus and Trametes villosa, show potential for future applications of synthetic sex hormones removal of contaminated waters and effluents.