Metabolismo do propionato em Paracoccidioides lutzii

Detalhes bibliográficos
Autor(a) principal: Santos, Luiz Paulo Araújo dos
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
dARK ID: ark:/38995/0013000002v48
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/8633
Resumo: Pathogens can find different carbon sources in host niches generating propionyl-CoA, among them propionate. This compound is toxic to the organism if accumulated within the cell, and can be generated in the host tissue by the metabolism of amino acids isoleucine, valine and methionine, or by the metabolism of odd-chain fatty acids. Therefore, during infection, the propionyl-CoA metabolism to nontoxic nutrient and usable energetically is of great relevance. Nonetheless, there are no studies about the propionyl-CoA metabolization pathway in fungi of the genus Paracoccidioides, causer of paracoccidioidomycosis, a systemic mycosis of high incidence in Latin America. Thus, to characterize of which metabolic pathway this fungus utilizes to propionyl-CoA metabolization, it was made a search the genes coding to enzymes of methylcitrate cycle in genome of Paracoccidioides spp. and were identified genes coding to the three exclusive enzymes of this cycle, which are methylcitrate synthase, methylcitrate dehydrogenase and methylcitrate lyase. After analysis of growth and viability, which demonstrated that Paracoccidioides lutzii utilizes propionate as carbon source, it was made gene expression analysis of enzymes of methylcitrate cycle and was observed that are regulated in response to propionate. Additionally, the enzymatic activity of the MCS showed that this enzyme is active inside of fungal cells and also when is secreted, as well as its dual capacity of to act with a citrate synthase and metylcitrate synthase. Finally, the proteomic profile of P. lutzii in propionate showed enzymes induction of methylcitrate cycle, glyoxylate cycle, amino acid metabolism and gluconeogenesis, and the repression of glycolytic pathway, fermentation and fatty acid synthesis, which demonstrated of metabolic rearrangement to supply the cellular energetic demand, metabolizing propionate. In this sense, to understand the mechanism of propionyl-CoA metabolism in P. lutzii provides data for visualization of metabolic adaptation that this fungus makes use in different colonization of niches.
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spelling Bailão, Alexandre Melohttp://lattes.cnpq.br/5415221996976886Bailão, Alexandre MeloBorges, Clayton LuizBailão, Elisa Flávia Luiz Cardosohttp://lattes.cnpq.br/6460945788659487Santos, Luiz Paulo Araújo dos2018-07-03T15:03:29Z2015-01-30SANTOS, L. P. A. Metabolismo do propionato em Paracoccidioides lutzii. 2015. 88 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/8633ark:/38995/0013000002v48Pathogens can find different carbon sources in host niches generating propionyl-CoA, among them propionate. This compound is toxic to the organism if accumulated within the cell, and can be generated in the host tissue by the metabolism of amino acids isoleucine, valine and methionine, or by the metabolism of odd-chain fatty acids. Therefore, during infection, the propionyl-CoA metabolism to nontoxic nutrient and usable energetically is of great relevance. Nonetheless, there are no studies about the propionyl-CoA metabolization pathway in fungi of the genus Paracoccidioides, causer of paracoccidioidomycosis, a systemic mycosis of high incidence in Latin America. Thus, to characterize of which metabolic pathway this fungus utilizes to propionyl-CoA metabolization, it was made a search the genes coding to enzymes of methylcitrate cycle in genome of Paracoccidioides spp. and were identified genes coding to the three exclusive enzymes of this cycle, which are methylcitrate synthase, methylcitrate dehydrogenase and methylcitrate lyase. After analysis of growth and viability, which demonstrated that Paracoccidioides lutzii utilizes propionate as carbon source, it was made gene expression analysis of enzymes of methylcitrate cycle and was observed that are regulated in response to propionate. Additionally, the enzymatic activity of the MCS showed that this enzyme is active inside of fungal cells and also when is secreted, as well as its dual capacity of to act with a citrate synthase and metylcitrate synthase. Finally, the proteomic profile of P. lutzii in propionate showed enzymes induction of methylcitrate cycle, glyoxylate cycle, amino acid metabolism and gluconeogenesis, and the repression of glycolytic pathway, fermentation and fatty acid synthesis, which demonstrated of metabolic rearrangement to supply the cellular energetic demand, metabolizing propionate. In this sense, to understand the mechanism of propionyl-CoA metabolism in P. lutzii provides data for visualization of metabolic adaptation that this fungus makes use in different colonization of niches.Micro-organismos patogênicos podem encontrar em nichos do hospedeiro diferentes fontes de carbono geradoras de propionil-CoA, dentre elas, o propionato. Este composto é tóxico para a célula se acumulado no seu interior, e pode ser gerado nos tecidos do hospedeiro pelo metabolismo dos aminoácidos isoleucina, valina e metionina, ou pelo metabolismo de ácidos graxos de cadeia ímpar. Portanto, durante a infecção, o metabolismo de propionil-CoA a um nutriente não tóxico e aproveitável energeticamente se faz de grande relevância. Contudo, não há estudos sobre a via de metabolização de propionil-CoA em fungo do gênero Paracoccidioides, causador da paracoccidioidomicose, uma micose sistêmica de alta incidência na América Latina. Sendo assim, para caracterização de qual via metabólica este organismo utiliza para metabolização de propionil-CoA, foi feita uma busca dos genes codificantes para enzimas do ciclo do metilcitrato no genoma de Paracoccidioides spp. e foram identificados genes codificantes para as três enzimas exclusivas desse ciclo, as quais são metilcitrato sintase, metilcitrato desidrogenase e metilcitrato liase. Após análise de crescimento e viabilidade, a qual demonstrou que Paracoccidioides lutzii utiliza propionato como fonte de carbono, foi feita a análise de expressão gênica das enzimas do ciclo do metilcitrato e observou-se que são reguladas em resposta ao propionato. Adicionalmente, a atividade enzimática da MCS demonstrou que essa enzima é ativa no interior da célula fúngica ou mesmo quando é secretada. Além disso, foi demostrada sua capacidade de atuar também como uma citrato sintase. Por fim, o perfil proteômico de P. lutzii em propionato mostrou a indução do ciclo do metilcitrato, ciclo do glioxilato, metabolismo de aminoácidos e gliconeogênese, e a repressão de vias como glicólise, fermentação e síntese de ácidos graxos, os quais demonstram o rearranjo metabólico para suprir a demanda energética celular metabolizando propionato. Neste sentido, entender os mecanismos pelo qual P. lutzii lança mão para metabolizar propionil-CoA permite a compreensão dos mecanismos de adaptação metabólica que esse fungo lança mão em diferentes nichos de colonização.Submitted by Erika Demachki (erikademachki@gmail.com) on 2018-06-29T18:20:13Z No. of bitstreams: 2 Dissertação - Luiz Paulo Araújo dos Santos - 2015.pdf: 1282200 bytes, checksum: ceda1b43513d7bf6756357d40f977117 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-07-03T15:03:29Z (GMT) No. of bitstreams: 2 Dissertação - Luiz Paulo Araújo dos Santos - 2015.pdf: 1282200 bytes, checksum: ceda1b43513d7bf6756357d40f977117 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-07-03T15:03:29Z (GMT). No. of bitstreams: 2 Dissertação - Luiz Paulo Araújo dos Santos - 2015.pdf: 1282200 bytes, checksum: ceda1b43513d7bf6756357d40f977117 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-01-30Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessParacoccidioides lutziiParacoccidioidomicoseCiclo do metilcitratoParacoccidioimycosisMethylcitrate cycleCIENCIAS BIOLOGICAS::MICROBIOLOGIAMetabolismo do propionato em Paracoccidioides lutziiMetabolism of propionate in Paracoccidioides lutziiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6085308344741430434600600600600-7769011444564556288-38545834699762208122075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.eng.fl_str_mv Metabolismo do propionato em Paracoccidioides lutzii
dc.title.alternative.eng.fl_str_mv Metabolism of propionate in Paracoccidioides lutzii
title Metabolismo do propionato em Paracoccidioides lutzii
spellingShingle Metabolismo do propionato em Paracoccidioides lutzii
Santos, Luiz Paulo Araújo dos
Paracoccidioides lutzii
Paracoccidioidomicose
Ciclo do metilcitrato
Paracoccidioimycosis
Methylcitrate cycle
CIENCIAS BIOLOGICAS::MICROBIOLOGIA
title_short Metabolismo do propionato em Paracoccidioides lutzii
title_full Metabolismo do propionato em Paracoccidioides lutzii
title_fullStr Metabolismo do propionato em Paracoccidioides lutzii
title_full_unstemmed Metabolismo do propionato em Paracoccidioides lutzii
title_sort Metabolismo do propionato em Paracoccidioides lutzii
author Santos, Luiz Paulo Araújo dos
author_facet Santos, Luiz Paulo Araújo dos
author_role author
dc.contributor.advisor1.fl_str_mv Bailão, Alexandre Melo
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5415221996976886
dc.contributor.referee1.fl_str_mv Bailão, Alexandre Melo
dc.contributor.referee2.fl_str_mv Borges, Clayton Luiz
dc.contributor.referee3.fl_str_mv Bailão, Elisa Flávia Luiz Cardoso
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6460945788659487
dc.contributor.author.fl_str_mv Santos, Luiz Paulo Araújo dos
contributor_str_mv Bailão, Alexandre Melo
Bailão, Alexandre Melo
Borges, Clayton Luiz
Bailão, Elisa Flávia Luiz Cardoso
dc.subject.por.fl_str_mv Paracoccidioides lutzii
Paracoccidioidomicose
Ciclo do metilcitrato
topic Paracoccidioides lutzii
Paracoccidioidomicose
Ciclo do metilcitrato
Paracoccidioimycosis
Methylcitrate cycle
CIENCIAS BIOLOGICAS::MICROBIOLOGIA
dc.subject.eng.fl_str_mv Paracoccidioimycosis
Methylcitrate cycle
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::MICROBIOLOGIA
description Pathogens can find different carbon sources in host niches generating propionyl-CoA, among them propionate. This compound is toxic to the organism if accumulated within the cell, and can be generated in the host tissue by the metabolism of amino acids isoleucine, valine and methionine, or by the metabolism of odd-chain fatty acids. Therefore, during infection, the propionyl-CoA metabolism to nontoxic nutrient and usable energetically is of great relevance. Nonetheless, there are no studies about the propionyl-CoA metabolization pathway in fungi of the genus Paracoccidioides, causer of paracoccidioidomycosis, a systemic mycosis of high incidence in Latin America. Thus, to characterize of which metabolic pathway this fungus utilizes to propionyl-CoA metabolization, it was made a search the genes coding to enzymes of methylcitrate cycle in genome of Paracoccidioides spp. and were identified genes coding to the three exclusive enzymes of this cycle, which are methylcitrate synthase, methylcitrate dehydrogenase and methylcitrate lyase. After analysis of growth and viability, which demonstrated that Paracoccidioides lutzii utilizes propionate as carbon source, it was made gene expression analysis of enzymes of methylcitrate cycle and was observed that are regulated in response to propionate. Additionally, the enzymatic activity of the MCS showed that this enzyme is active inside of fungal cells and also when is secreted, as well as its dual capacity of to act with a citrate synthase and metylcitrate synthase. Finally, the proteomic profile of P. lutzii in propionate showed enzymes induction of methylcitrate cycle, glyoxylate cycle, amino acid metabolism and gluconeogenesis, and the repression of glycolytic pathway, fermentation and fatty acid synthesis, which demonstrated of metabolic rearrangement to supply the cellular energetic demand, metabolizing propionate. In this sense, to understand the mechanism of propionyl-CoA metabolism in P. lutzii provides data for visualization of metabolic adaptation that this fungus makes use in different colonization of niches.
publishDate 2015
dc.date.issued.fl_str_mv 2015-01-30
dc.date.accessioned.fl_str_mv 2018-07-03T15:03:29Z
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dc.identifier.citation.fl_str_mv SANTOS, L. P. A. Metabolismo do propionato em Paracoccidioides lutzii. 2015. 88 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2015.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/8633
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identifier_str_mv SANTOS, L. P. A. Metabolismo do propionato em Paracoccidioides lutzii. 2015. 88 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2015.
ark:/38995/0013000002v48
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