Caracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismo
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
dARK ID: | ark:/38995/0013000006fq0 |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/5000 |
Resumo: | Fungi of the genus Trichoderma have emerged as biological control agents, especially those belonging to the species Trichoderma harzianum with various mechanisms involved include production of antibiotics, mycoparasitism, competition for nutrients and host plant resistance induction. Mycoparasitism is a sequential process that involves recognition of the host contact, adhesion, expression of specific genes and secretion of secondary metabolites. Several hydrolytic enzymes are involved in this process among them chitinases, beta-glucanases and proteases. Recent studies of T. harzianum interaction with some pathogens have shown that other enzymes may also be involved in this mechanism, among which alphamannosidase. Alpha-mannosidase has an essential role in the recycling of glycoproteins in filamentous fungi, but little is known about the role of these enzymes in mycoparasitism. This study aims to characterize the expression of T. alphamannosidase gene harzianum after growth in cell wall of Sclerotinia sclerotiorum (SS), Fusarium solani (FS) and Fusarium oxysporum (FO) as well as during the interaction in vivo . It was used as a control cell wall T. harzianum (TH). Also, jobs obtaining mutants of this gene in T. harzianum were performed. Our data show that alpha-mannosidase gene was highly expressed as T. harzianum grown in the presence of cell wall SS (4.5X), FS (18X) and FO (75X) compared to the control TH (1X). In comparison situation it was noticed a 75X greater expression in TH x FO state during contact as compared with the control (TH x TH). In other situations of confrontation did not observe a significant increase in expression. The promoter region, the selection mark and the terminator region of the alpha-mannosidase of T. harzianum gene was cloned into the pRS426 vector, and the deletion cassette was assembled by the recombination technique in Saccharomyces cerevisiae. Then, these vectors were used for transformation of T. harzianum and transformants were obtained by selection on plates containing hygromycin b. Approximately 50 transformants were obtained, but none of the alpha-mannosidase gene was successfully deleted. Obtaining more transformants is necessary for us to have a mutant alpha-mannosidase gene deleted. |
id |
UFG-2_a7c8f29121a006ecbca60a465a8c8ce0 |
---|---|
oai_identifier_str |
oai:repositorio.bc.ufg.br:tede/5000 |
network_acronym_str |
UFG-2 |
network_name_str |
Repositório Institucional da UFG |
repository_id_str |
|
spelling |
Ulhoa, Cirano Joséhttp://lattes.cnpq.br/8368469162867277Silva, Roberto do Nascimentohttp://lattes.cnpq.br/5771309284349109Ulhoa, Cirano JoséVieira, Pabline MarinhoGeorg, Raphaela de Castrohttp://lattes.cnpq.br/7089252809031211Mota, Paulo Roberto da2015-12-04T10:27:57Z2015-08-28MOTA, P. R. Caracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismo. 2015. 54 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/5000ark:/38995/0013000006fq0Fungi of the genus Trichoderma have emerged as biological control agents, especially those belonging to the species Trichoderma harzianum with various mechanisms involved include production of antibiotics, mycoparasitism, competition for nutrients and host plant resistance induction. Mycoparasitism is a sequential process that involves recognition of the host contact, adhesion, expression of specific genes and secretion of secondary metabolites. Several hydrolytic enzymes are involved in this process among them chitinases, beta-glucanases and proteases. Recent studies of T. harzianum interaction with some pathogens have shown that other enzymes may also be involved in this mechanism, among which alphamannosidase. Alpha-mannosidase has an essential role in the recycling of glycoproteins in filamentous fungi, but little is known about the role of these enzymes in mycoparasitism. This study aims to characterize the expression of T. alphamannosidase gene harzianum after growth in cell wall of Sclerotinia sclerotiorum (SS), Fusarium solani (FS) and Fusarium oxysporum (FO) as well as during the interaction in vivo . It was used as a control cell wall T. harzianum (TH). Also, jobs obtaining mutants of this gene in T. harzianum were performed. Our data show that alpha-mannosidase gene was highly expressed as T. harzianum grown in the presence of cell wall SS (4.5X), FS (18X) and FO (75X) compared to the control TH (1X). In comparison situation it was noticed a 75X greater expression in TH x FO state during contact as compared with the control (TH x TH). In other situations of confrontation did not observe a significant increase in expression. The promoter region, the selection mark and the terminator region of the alpha-mannosidase of T. harzianum gene was cloned into the pRS426 vector, and the deletion cassette was assembled by the recombination technique in Saccharomyces cerevisiae. Then, these vectors were used for transformation of T. harzianum and transformants were obtained by selection on plates containing hygromycin b. Approximately 50 transformants were obtained, but none of the alpha-mannosidase gene was successfully deleted. Obtaining more transformants is necessary for us to have a mutant alpha-mannosidase gene deleted.Fungos do gênero Trichoderma têm se destacado como agentes de controle biológico, principalmente aqueles pertencentes à espécie Trichoderma harzianumcom vários mecanismos envolvidos incluem produção de antibióticos, micoparasitismo, competição por nutrientes e indução de resistência da planta hospedeira. Micoparasitismo é um processo sequencial que envolve reconhecimento do hospedeiro, contato, adesão, expressão de genes específicos e secreção de metabólitos secundários. Várias enzimas hidrolíticas estão envolvidas neste processo dentre elas quitinases, beta-glucanases e proteases. Estudos recentes de interação de T. harzianum com alguns fitopatógenos tem demonstrado que outras enzimas podem também estar envolvidas neste mecanismo, dentre elas as alfamanosidases. As alfa-manosidases tem um papel fundamental na reciclagem de glicoproteínas em fungos filamentosos, mas pouco se sabe sobre o papel destas enzimas no micoparasitismo. Este trabalho tem como objetivo a caracterização da expressão do gene de alfa-manosidase de T. harzianumapós crescimento em parede celular de Sclerotinia sclerotiorum (SS), Fusarium solani (FS) e Fusarium oxysporum (FO), bem como durante a interação in vivo. Como controle foi utilizado parede celular de T. harzianum (TH). Além disto, trabalhos de obtenção de mutantes deste gene em T. harzianum foram realizados. Nossos dados mostraram que o gene de alfa-manosidase foi altamente expresso quando T. harzianum cresceu na presença de parede celular de SS (4.5X), FS (18X) e FO (75X) quando comparado com o controle TH (1X). Em situação de confronto foi observado uma expressão de 75X maior na situação TH x FO durante o contato, quando comparado com o controle (TH x TH). Na outras situações de confronto não observamos um aumento significativo de expressão. A região promotora, a marca de seleção e a região terminadora do gene da alfa-manosidase de T. harzianumfoi clonado no vetor pRS426 e o cassete de deleção foi montado através da técnica de recombinação em Saccharomyces cerevisiae . Em seguida, estes vetores foram utilizados para a transformação de T. harzianume os transformantes foram obtidos por seleção em placas contendo higromicina b. Aproximadamente 50 transformantes foram obtidos, mas em nenhum deles o gene da alfa-manosidase foi deletado com sucesso. A obtenção de mais transformantes se faz necessário para que possamos ter um mutante com o gene da alfa-manosidase deletado.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2015-12-04T10:24:50Z No. of bitstreams: 2 Dissertação - Paulo Roberto da Mota - 2015.pdf: 1806908 bytes, checksum: d6798b75ceeef2f456ddde6fbf387e77 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-12-04T10:27:57Z (GMT) No. of bitstreams: 2 Dissertação - Paulo Roberto da Mota - 2015.pdf: 1806908 bytes, checksum: d6798b75ceeef2f456ddde6fbf387e77 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2015-12-04T10:27:57Z (GMT). No. of bitstreams: 2 Dissertação - Paulo Roberto da Mota - 2015.pdf: 1806908 bytes, checksum: d6798b75ceeef2f456ddde6fbf387e77 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2015-08-28application/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessFitopatógenosMicoparasitismoCompetiçãoParede celular e expressãoPlant pathogensMycoparasitismCompetitionCcellular wall and expressionCIENCIAS BIOLOGICAS::BIOLOGIA GERALCaracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6883982777473437920600600600-3872772117827373404-1634559385931244697reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://repositorio.bc.ufg.br/tede/bitstreams/8ecabf2b-2923-4e96-b468-71c0e261cbef/downloadbd3efa91386c1718a7f26a329fdcb468MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.bc.ufg.br/tede/bitstreams/16dc8033-5a31-41f9-912f-b90bee8834b7/download4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-821468http://repositorio.bc.ufg.br/tede/bitstreams/a222949d-8671-470d-bd39-54418ae6e4b8/downloadae2fe251842ade1134c5d9bb99b6eefeMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-823148http://repositorio.bc.ufg.br/tede/bitstreams/ea54cd45-4d74-4d7e-a0a8-f99ff5432c10/download9da0b6dfac957114c6a7714714b86306MD54ORIGINALDissertação - Paulo Roberto da Mota - 2015.pdfDissertação - Paulo Roberto da Mota - 2015.pdfapplication/pdf1806908http://repositorio.bc.ufg.br/tede/bitstreams/a9d19740-92c1-41c9-b050-b83a5695323a/downloadd6798b75ceeef2f456ddde6fbf387e77MD55tede/50002015-12-04 08:27:57.962http://creativecommons.org/licenses/by-nc-nd/4.0/Acesso Abertoopen.accessoai:repositorio.bc.ufg.br:tede/5000http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2015-12-04T10:27:57Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.por.fl_str_mv |
Caracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismo |
title |
Caracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismo |
spellingShingle |
Caracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismo Mota, Paulo Roberto da Fitopatógenos Micoparasitismo Competição Parede celular e expressão Plant pathogens Mycoparasitism Competition Ccellular wall and expression CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Caracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismo |
title_full |
Caracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismo |
title_fullStr |
Caracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismo |
title_full_unstemmed |
Caracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismo |
title_sort |
Caracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismo |
author |
Mota, Paulo Roberto da |
author_facet |
Mota, Paulo Roberto da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ulhoa, Cirano José |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8368469162867277 |
dc.contributor.advisor-co1.fl_str_mv |
Silva, Roberto do Nascimento |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/5771309284349109 |
dc.contributor.referee1.fl_str_mv |
Ulhoa, Cirano José |
dc.contributor.referee2.fl_str_mv |
Vieira, Pabline Marinho |
dc.contributor.referee3.fl_str_mv |
Georg, Raphaela de Castro |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7089252809031211 |
dc.contributor.author.fl_str_mv |
Mota, Paulo Roberto da |
contributor_str_mv |
Ulhoa, Cirano José Silva, Roberto do Nascimento Ulhoa, Cirano José Vieira, Pabline Marinho Georg, Raphaela de Castro |
dc.subject.por.fl_str_mv |
Fitopatógenos Micoparasitismo Competição Parede celular e expressão |
topic |
Fitopatógenos Micoparasitismo Competição Parede celular e expressão Plant pathogens Mycoparasitism Competition Ccellular wall and expression CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Plant pathogens Mycoparasitism Competition Ccellular wall and expression |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Fungi of the genus Trichoderma have emerged as biological control agents, especially those belonging to the species Trichoderma harzianum with various mechanisms involved include production of antibiotics, mycoparasitism, competition for nutrients and host plant resistance induction. Mycoparasitism is a sequential process that involves recognition of the host contact, adhesion, expression of specific genes and secretion of secondary metabolites. Several hydrolytic enzymes are involved in this process among them chitinases, beta-glucanases and proteases. Recent studies of T. harzianum interaction with some pathogens have shown that other enzymes may also be involved in this mechanism, among which alphamannosidase. Alpha-mannosidase has an essential role in the recycling of glycoproteins in filamentous fungi, but little is known about the role of these enzymes in mycoparasitism. This study aims to characterize the expression of T. alphamannosidase gene harzianum after growth in cell wall of Sclerotinia sclerotiorum (SS), Fusarium solani (FS) and Fusarium oxysporum (FO) as well as during the interaction in vivo . It was used as a control cell wall T. harzianum (TH). Also, jobs obtaining mutants of this gene in T. harzianum were performed. Our data show that alpha-mannosidase gene was highly expressed as T. harzianum grown in the presence of cell wall SS (4.5X), FS (18X) and FO (75X) compared to the control TH (1X). In comparison situation it was noticed a 75X greater expression in TH x FO state during contact as compared with the control (TH x TH). In other situations of confrontation did not observe a significant increase in expression. The promoter region, the selection mark and the terminator region of the alpha-mannosidase of T. harzianum gene was cloned into the pRS426 vector, and the deletion cassette was assembled by the recombination technique in Saccharomyces cerevisiae. Then, these vectors were used for transformation of T. harzianum and transformants were obtained by selection on plates containing hygromycin b. Approximately 50 transformants were obtained, but none of the alpha-mannosidase gene was successfully deleted. Obtaining more transformants is necessary for us to have a mutant alpha-mannosidase gene deleted. |
publishDate |
2015 |
dc.date.accessioned.fl_str_mv |
2015-12-04T10:27:57Z |
dc.date.issued.fl_str_mv |
2015-08-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MOTA, P. R. Caracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismo. 2015. 54 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/5000 |
dc.identifier.dark.fl_str_mv |
ark:/38995/0013000006fq0 |
identifier_str_mv |
MOTA, P. R. Caracterizaçãoda alfa-manosidase produzida por Trichoderma harzianum durante o micoparasitismo. 2015. 54 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2015. ark:/38995/0013000006fq0 |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/5000 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
6883982777473437920 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
-3872772117827373404 |
dc.relation.cnpq.fl_str_mv |
-1634559385931244697 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Biologia (ICB) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Ciências Biológicas - ICB (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/8ecabf2b-2923-4e96-b468-71c0e261cbef/download http://repositorio.bc.ufg.br/tede/bitstreams/16dc8033-5a31-41f9-912f-b90bee8834b7/download http://repositorio.bc.ufg.br/tede/bitstreams/a222949d-8671-470d-bd39-54418ae6e4b8/download http://repositorio.bc.ufg.br/tede/bitstreams/ea54cd45-4d74-4d7e-a0a8-f99ff5432c10/download http://repositorio.bc.ufg.br/tede/bitstreams/a9d19740-92c1-41c9-b050-b83a5695323a/download |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f ae2fe251842ade1134c5d9bb99b6eefe 9da0b6dfac957114c6a7714714b86306 d6798b75ceeef2f456ddde6fbf387e77 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1815172578437758976 |