Salmonella sp. em ovos e patos (Cairina moschata) de criações informais
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/6945 |
Resumo: | The survey was conducted in order to investigate the presence of Salmonella sp. in swabs of cloaca and duck eggs coming from 38 informal breeding sites and 38 markets, and to detect anti-Salmonella Gallinarum and Pullorum antibodies in blood serum of these birds, in the State of Goiás and the Federal Distrito. To determine the productive and sanitary profile of the farms where ducks are raised, we applied 38 questionnaires consisting of 12 closed questions. Through the information obtained by the responses to the questionnaires during the visits, we observed that the farms of the studied regions did not present adequate productive and sanitary management. We collected 324 whole blood samples were used in the test Serological Rapid Agglutination plate (SAR) to detect anti-Salmonella pullorum antibodies. In this test 117/324 (36.1%) blood sera were seropositive. We harvested 324 cloacal swabs from ducks and 912 eggs from 38 farms and 38 markets. The samples were subdivided according to the structure in pools, producing 228 eggshell samples, 228 albumen samples and 228 yolk samples, totaling 684 samples. Cloacal swabs and eggs were analyzed by conventional bacteriology for Salmonella sp. We obtained 38 isolates, 36 from eggs in 36/684 (5.3%), and the serovars were Salmonella Heidelberg, Schwarzengrund, antigenic form O: 9.12, and Salmonella typhimurium, and two isolates from cloaca swabs 02/324 (0.62%). The identified serovars were Salmonella Typhimurium and Hadar. The 36 isolates from eggs and two from cloacal swabs were submitted to susceptibility test to 15 antimicrobials, from which we obtained the following distribution of resistance: 29/38 (76.3%) to sulfonomide, 14/38 (36, 9%) to enroflaxacin, 10/38 (26.3%) to amoxicillin, 7/38 (18.4%) to ampicillin, 7/38 (18.4%) to gentamicin, 6/38 (15.8%) to cotrimoxazole, 6/38 (15.8%) to cephalothin, 6/38 (15.8%) to trimethoprim, 03/38 (7.9%) to ceftiofur, 05/38 (13.1 %) to neomycin, 5/38 (13.1%) to tetracycline, 5/38 (13.1%) to fosfomycin, 3/38 (7.9%) to chloramphenicol, 2/38 (5.3%) to ciprofloxacin, and 0/38 (0%) to florfenicol. The percentage distribution of the isolated serovars was: 32/38 (84.2%) Salmonella Schwarzengrund, 3/38 (7.9%) Salmonella Typhimurium, 1/38 (2.6%) the antigen form: 9.12, 1/38 (2.6%) Salmonella Heidelberg, and 1/38 (2.6%) Salmonella Hadar. The 36 isolates of Salmonella were also submitted to proof of real-time polymerase chain reaction (real-time PCR) on which the following genes were detected: 33/35 (94.3%) for rfbJ, 07/35 (20%) for Int1 gene, and 06/35 (17,1%) for Sul1 gene. Therefore, we verified circulation of Salmonella in ducks and their eggs in informal rearing farms in the state of Goiás and in the Federal District, we also confirmed the antimicrobial resistance and detected resistance genes. This information is important for determining risk for both poultry and public health. |
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Jayme, Valéria de Sáhttp://lattes.cnpq.br/0603234425928309Andrade, Maria Auxiliadorahttp://lattes.cnpq.br/9441751521255467Jayme, Valéria de Sáhttp://lattes.cnpq.br/0603234425928309Meireles, Marcelo VasconcelosAndré, Maria Cláudia Dantas Porfírio BorgesMoraes, Dunya Mara CardosoResende, Cintia S. Minafra dehttp://lattes.cnpq.br/3613389906366977Delfino, Denizard André de Abreu2017-03-20T12:43:06Z2016-10-07DELFINO, D. A. A. Salmonella sp. em ovos e patos (Cairina moschata) de criações informais. 2016. 74 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016.http://repositorio.bc.ufg.br/tede/handle/tede/6945ark:/38995/0013000001fdnThe survey was conducted in order to investigate the presence of Salmonella sp. in swabs of cloaca and duck eggs coming from 38 informal breeding sites and 38 markets, and to detect anti-Salmonella Gallinarum and Pullorum antibodies in blood serum of these birds, in the State of Goiás and the Federal Distrito. To determine the productive and sanitary profile of the farms where ducks are raised, we applied 38 questionnaires consisting of 12 closed questions. Through the information obtained by the responses to the questionnaires during the visits, we observed that the farms of the studied regions did not present adequate productive and sanitary management. We collected 324 whole blood samples were used in the test Serological Rapid Agglutination plate (SAR) to detect anti-Salmonella pullorum antibodies. In this test 117/324 (36.1%) blood sera were seropositive. We harvested 324 cloacal swabs from ducks and 912 eggs from 38 farms and 38 markets. The samples were subdivided according to the structure in pools, producing 228 eggshell samples, 228 albumen samples and 228 yolk samples, totaling 684 samples. Cloacal swabs and eggs were analyzed by conventional bacteriology for Salmonella sp. We obtained 38 isolates, 36 from eggs in 36/684 (5.3%), and the serovars were Salmonella Heidelberg, Schwarzengrund, antigenic form O: 9.12, and Salmonella typhimurium, and two isolates from cloaca swabs 02/324 (0.62%). The identified serovars were Salmonella Typhimurium and Hadar. The 36 isolates from eggs and two from cloacal swabs were submitted to susceptibility test to 15 antimicrobials, from which we obtained the following distribution of resistance: 29/38 (76.3%) to sulfonomide, 14/38 (36, 9%) to enroflaxacin, 10/38 (26.3%) to amoxicillin, 7/38 (18.4%) to ampicillin, 7/38 (18.4%) to gentamicin, 6/38 (15.8%) to cotrimoxazole, 6/38 (15.8%) to cephalothin, 6/38 (15.8%) to trimethoprim, 03/38 (7.9%) to ceftiofur, 05/38 (13.1 %) to neomycin, 5/38 (13.1%) to tetracycline, 5/38 (13.1%) to fosfomycin, 3/38 (7.9%) to chloramphenicol, 2/38 (5.3%) to ciprofloxacin, and 0/38 (0%) to florfenicol. The percentage distribution of the isolated serovars was: 32/38 (84.2%) Salmonella Schwarzengrund, 3/38 (7.9%) Salmonella Typhimurium, 1/38 (2.6%) the antigen form: 9.12, 1/38 (2.6%) Salmonella Heidelberg, and 1/38 (2.6%) Salmonella Hadar. The 36 isolates of Salmonella were also submitted to proof of real-time polymerase chain reaction (real-time PCR) on which the following genes were detected: 33/35 (94.3%) for rfbJ, 07/35 (20%) for Int1 gene, and 06/35 (17,1%) for Sul1 gene. Therefore, we verified circulation of Salmonella in ducks and their eggs in informal rearing farms in the state of Goiás and in the Federal District, we also confirmed the antimicrobial resistance and detected resistance genes. This information is important for determining risk for both poultry and public health.A pesquisa foi feita com o objetivo de investigar a presença de Salmonella sp. em suabes de cloaca e nos ovos de patas oriundos de 38 criações informais e de 38 feiras livres, além de detectar anticorpos anti-Samonella Gallinarum e Pullorum em soro sanguíneo destas aves, no Estado de Goiás e no Distrito Federal. Para determinação do perfil produtivo e sanitário destas propriedades que criavam patos foram aplicados 38 questionários compostos por 12 perguntas fechadas. Mediante as informações obtidas pelas respostas dos questionários aplicados durante as visitas, foi observado que as propriedades das regiões estudadas não apresentavam manejo produtivo e sanitário adequados. Foram colhidas 324 amostras de sangue total que foram utilizadas na prova Sorológica de Aglutinação Rápida em Placa (SAR), para detecção de anticorpos anti-Salmonella Pullorum e Gallinarum. Nesta prova 117/324 (36,1%) dos soros sanguíneos foram sororreagentes. Foram colhidas 324 suabes cloacais de patos e 912 ovos em 38 propriedades e 38 feiras, que foram subdivididos de acordo com a estrutura em pool, dando origem a 228 amostras de casca, 228 de albúmen e 228 de gemas, totalizando 684 amostras. Os suabes cloacais e ovos foram analisados por bacteriologia convencional para pesquisa de Salmonella sp. Foram obtidos 38 isolados, sendo 36 em ovos 36/684 (5,3%), onde os sorovares foram Salmonella Heidelberg, Schwarzengrund, forma antigênica O:9,12 e Salmonella Typhimurium e dois isolados de suabes de cloaca 02/324 (0,62%), sendo identificados os sorovares Salmonella Typhimurium e Hadar. A distribuição percentual dos sorovares isolados foi: 32/38 (84,2%) Salmonella Schwarzengrund, 3/38 (7,9%) Salmonella Typhimurium, 1/38 (2,6%) a forma antigênica O:9,12, 1/38 (2,6%) Salmonella Heidelberg e 1/38 (2,6%) Salmonella Hadar. Os 36 isolados de ovos e os dois de suabes cloacais foram submetidos aos testes de suscetibilidade a 15 antimicrobianos, nos quais se obteve a seguinte distribuição de resistência: 29/38 (76,3%,) às sulfonamidas, 14/38 (36,9%) à enroflaxacina, 10/38 (26,3%) à amoxicilina, 7/38 (18,4%) à ampicilina, 7/38 (18,4%) à gentamicina, 6/38 (15,8%) ao cotrimoxazol, 6/38 (15,8%) à cefalotina, 6/38 (15,8%) ao trimetoprim, 03/38 (7,9%) ao ceftiofur, 05/38 (13,1%) a neomicina, 5/38 (13,1%) à tetraciclina, 5/38 (13,1%) à fosfomicina, 3/38 (7,9%) ao cloranfenicol, 2/38 (5,3%) à ciprofloxacina e 0/38 (0%) ao florfenicol. Os 36 isolados de Salmonella foram ainda submetidos a prova de PCR em tempo real, onde foram detectados os seguintes genes: rfbJ 33/35 (94,3%), o gene Int1 foi de 07/35 (20%) e o gene Sul1 foi de 06/35 (17,1%). Portanto, foi detectada a circulação de Salmonella em patos e seus ovos nas criações informais e feiras no Estado de Goiás e no Distrito Federal, assim como a confirmação de resistência aos antimicrobianos e detecção de genes de resistência. Estas informações são importantes para determinação de risco, tanto para sanidade avícola quanto para saúde pública.Submitted by JÚLIO HEBER SILVA (julioheber@yahoo.com.br) on 2017-03-15T19:00:03Z No. of bitstreams: 2 Tese - Denizard André de Abreu Delfino - 2016.pdf: 1856323 bytes, checksum: cf3dc822049bf13dcac10982b702dfc6 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-03-20T12:43:06Z (GMT) No. of bitstreams: 2 Tese - Denizard André de Abreu Delfino - 2016.pdf: 1856323 bytes, checksum: cf3dc822049bf13dcac10982b702dfc6 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2017-03-20T12:43:06Z (GMT). No. of bitstreams: 2 Tese - Denizard André de Abreu Delfino - 2016.pdf: 1856323 bytes, checksum: cf3dc822049bf13dcac10982b702dfc6 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-10-07Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESConselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciência Animal (EVZ)UFGBrasilEscola de Veterinária e Zootecnia - EVZ (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAntimicrobianosGenesPropriedadesPCRResistênciaAntimicrobialsFacilitiesGenesPCRResistanceMEDICINA VETERINARIA PREVENTIVA::DOENCAS PARASITARIAS DE ANIMAISSalmonella sp. em ovos e patos (Cairina moschata) de criações informaisSalmonella sp. in eggs and ducks (Cairina moschata) from informative creationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis4581960685150189167600600600600600-621755211424909458210814403748859493862075167498588264571-2555911436985713659reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Salmonella sp. em ovos e patos (Cairina moschata) de criações informais |
dc.title.alternative.eng.fl_str_mv |
Salmonella sp. in eggs and ducks (Cairina moschata) from informative creations |
title |
Salmonella sp. em ovos e patos (Cairina moschata) de criações informais |
spellingShingle |
Salmonella sp. em ovos e patos (Cairina moschata) de criações informais Delfino, Denizard André de Abreu Antimicrobianos Genes Propriedades PCR Resistência Antimicrobials Facilities Genes PCR Resistance MEDICINA VETERINARIA PREVENTIVA::DOENCAS PARASITARIAS DE ANIMAIS |
title_short |
Salmonella sp. em ovos e patos (Cairina moschata) de criações informais |
title_full |
Salmonella sp. em ovos e patos (Cairina moschata) de criações informais |
title_fullStr |
Salmonella sp. em ovos e patos (Cairina moschata) de criações informais |
title_full_unstemmed |
Salmonella sp. em ovos e patos (Cairina moschata) de criações informais |
title_sort |
Salmonella sp. em ovos e patos (Cairina moschata) de criações informais |
author |
Delfino, Denizard André de Abreu |
author_facet |
Delfino, Denizard André de Abreu |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Jayme, Valéria de Sá |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0603234425928309 |
dc.contributor.advisor-co1.fl_str_mv |
Andrade, Maria Auxiliadora |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/9441751521255467 |
dc.contributor.referee1.fl_str_mv |
Jayme, Valéria de Sá |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/0603234425928309 |
dc.contributor.referee2.fl_str_mv |
Meireles, Marcelo Vasconcelos |
dc.contributor.referee3.fl_str_mv |
André, Maria Cláudia Dantas Porfírio Borges |
dc.contributor.referee4.fl_str_mv |
Moraes, Dunya Mara Cardoso |
dc.contributor.referee5.fl_str_mv |
Resende, Cintia S. Minafra de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3613389906366977 |
dc.contributor.author.fl_str_mv |
Delfino, Denizard André de Abreu |
contributor_str_mv |
Jayme, Valéria de Sá Andrade, Maria Auxiliadora Jayme, Valéria de Sá Meireles, Marcelo Vasconcelos André, Maria Cláudia Dantas Porfírio Borges Moraes, Dunya Mara Cardoso Resende, Cintia S. Minafra de |
dc.subject.por.fl_str_mv |
Antimicrobianos Genes Propriedades PCR Resistência |
topic |
Antimicrobianos Genes Propriedades PCR Resistência Antimicrobials Facilities Genes PCR Resistance MEDICINA VETERINARIA PREVENTIVA::DOENCAS PARASITARIAS DE ANIMAIS |
dc.subject.eng.fl_str_mv |
Antimicrobials Facilities Genes PCR Resistance |
dc.subject.cnpq.fl_str_mv |
MEDICINA VETERINARIA PREVENTIVA::DOENCAS PARASITARIAS DE ANIMAIS |
description |
The survey was conducted in order to investigate the presence of Salmonella sp. in swabs of cloaca and duck eggs coming from 38 informal breeding sites and 38 markets, and to detect anti-Salmonella Gallinarum and Pullorum antibodies in blood serum of these birds, in the State of Goiás and the Federal Distrito. To determine the productive and sanitary profile of the farms where ducks are raised, we applied 38 questionnaires consisting of 12 closed questions. Through the information obtained by the responses to the questionnaires during the visits, we observed that the farms of the studied regions did not present adequate productive and sanitary management. We collected 324 whole blood samples were used in the test Serological Rapid Agglutination plate (SAR) to detect anti-Salmonella pullorum antibodies. In this test 117/324 (36.1%) blood sera were seropositive. We harvested 324 cloacal swabs from ducks and 912 eggs from 38 farms and 38 markets. The samples were subdivided according to the structure in pools, producing 228 eggshell samples, 228 albumen samples and 228 yolk samples, totaling 684 samples. Cloacal swabs and eggs were analyzed by conventional bacteriology for Salmonella sp. We obtained 38 isolates, 36 from eggs in 36/684 (5.3%), and the serovars were Salmonella Heidelberg, Schwarzengrund, antigenic form O: 9.12, and Salmonella typhimurium, and two isolates from cloaca swabs 02/324 (0.62%). The identified serovars were Salmonella Typhimurium and Hadar. The 36 isolates from eggs and two from cloacal swabs were submitted to susceptibility test to 15 antimicrobials, from which we obtained the following distribution of resistance: 29/38 (76.3%) to sulfonomide, 14/38 (36, 9%) to enroflaxacin, 10/38 (26.3%) to amoxicillin, 7/38 (18.4%) to ampicillin, 7/38 (18.4%) to gentamicin, 6/38 (15.8%) to cotrimoxazole, 6/38 (15.8%) to cephalothin, 6/38 (15.8%) to trimethoprim, 03/38 (7.9%) to ceftiofur, 05/38 (13.1 %) to neomycin, 5/38 (13.1%) to tetracycline, 5/38 (13.1%) to fosfomycin, 3/38 (7.9%) to chloramphenicol, 2/38 (5.3%) to ciprofloxacin, and 0/38 (0%) to florfenicol. The percentage distribution of the isolated serovars was: 32/38 (84.2%) Salmonella Schwarzengrund, 3/38 (7.9%) Salmonella Typhimurium, 1/38 (2.6%) the antigen form: 9.12, 1/38 (2.6%) Salmonella Heidelberg, and 1/38 (2.6%) Salmonella Hadar. The 36 isolates of Salmonella were also submitted to proof of real-time polymerase chain reaction (real-time PCR) on which the following genes were detected: 33/35 (94.3%) for rfbJ, 07/35 (20%) for Int1 gene, and 06/35 (17,1%) for Sul1 gene. Therefore, we verified circulation of Salmonella in ducks and their eggs in informal rearing farms in the state of Goiás and in the Federal District, we also confirmed the antimicrobial resistance and detected resistance genes. This information is important for determining risk for both poultry and public health. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-10-07 |
dc.date.accessioned.fl_str_mv |
2017-03-20T12:43:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
DELFINO, D. A. A. Salmonella sp. em ovos e patos (Cairina moschata) de criações informais. 2016. 74 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/6945 |
dc.identifier.dark.fl_str_mv |
ark:/38995/0013000001fdn |
identifier_str_mv |
DELFINO, D. A. A. Salmonella sp. em ovos e patos (Cairina moschata) de criações informais. 2016. 74 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016. ark:/38995/0013000001fdn |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/6945 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
4581960685150189167 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 |
dc.relation.department.fl_str_mv |
-6217552114249094582 |
dc.relation.cnpq.fl_str_mv |
1081440374885949386 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 -2555911436985713659 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Ciência Animal (EVZ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Veterinária e Zootecnia - EVZ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
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repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
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1811721335438573568 |