Padronização de dose de tetracloreto de carbono em modelo de lesão hepática aguda por estresse oxidativo em ratos Wistar
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/4309 |
Resumo: | The carbon tetrachloride (CCl4) is recognized as a classic hepatotoxin, being considered the best method to induce liver injury, commonly used as a model to test the hepatoprotective effect of drugs and natural substances and for investigation of hepatotoxicity, cytotoxicity and oxidative stress, in both in vivo and in vitro studies. This study aimed to standardize the lowest dose of CCl4 to cause acute liver injury by oxidative stress in Wistar rats. The in vivo experiment was carried out with 12 male Wistar rats (180-240g),which were maintained for 16 days under controlled environment and supplied with water and Purina® rodent ad libitum. The animals were separated into four groups: CG - control group; G0,5 - dose of 0.5 ml / kg body weight (bw); G0,75 - dose of 0.75 ml / kg bw and G1 - dose of 1 ml / kg bw. CCl4 was administered by intraperitoneal injection twice a week. 24h after the last CCl4 administration, all animals were anesthetized (xylazine: ketamine - 1: 1 v / v) for performing cardiac puncture, euthanasia and dissection of the liver for removal and analysis. Results obtained were subjected to normality test, and followed by the analysis of variance (ANOVA) and comparison of means (Tukey at 5% probability - post-hoc). It was observed that the mean weights of rats treated with CCl4 were higher than that of to the GC and the liver protein content (in g / 100 g) was lower in the treated groups, with no statistical difference between the test groups. Histopathological analysis showed changes in the structure, increased numbers of macrophages with intracellular lipid accumulation and increased cellular infiltration in groups treated with CCl4. By quantifying the enzymes alanine aminotransferase (ALT), aspartate and alanine aminotransferase (AST), it was found that the CCl4-treated groups showed significantly higher levels than CG, being higher in G1 compared to the other groups tested. All tested concentrations of CCl4 induced liver injury in vivo, in different degrees, and the concentration of 0.5 mL / kg bw, administered twice weekly, was the lowest dose tested that could cause changes in all the parameters evaluated. Therefore, this is the ideal dose for induction of acute liver injury, aiming to test the modulatory role of dietary and nutritional factors. |
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Naves, Maria Margareth Veloso http://lattes.cnpq.br/6563181057140270Cominetti, CristianeHorst, Maria AderuzaNaves, Maria Margareth VelosoCeles, Mara Rúbia Nuneshttp://lattes.cnpq.br/9945884989330825Vieira, Bárbara Martins2015-03-20T14:04:37Z2014-08-22VIEIRA, B. M. Padronização de dose de tetracloreto de carbono em modelo de lesão hepática aguda por estresse oxidativo em ratos Wistar. 2014. 53 f. Dissertação ( Mestrado em Nutrição e Saúde) - Universidade Federal de Goiás, Goiânia, 2014.http://repositorio.bc.ufg.br/tede/handle/tede/4309ark:/38995/0013000001srqThe carbon tetrachloride (CCl4) is recognized as a classic hepatotoxin, being considered the best method to induce liver injury, commonly used as a model to test the hepatoprotective effect of drugs and natural substances and for investigation of hepatotoxicity, cytotoxicity and oxidative stress, in both in vivo and in vitro studies. This study aimed to standardize the lowest dose of CCl4 to cause acute liver injury by oxidative stress in Wistar rats. The in vivo experiment was carried out with 12 male Wistar rats (180-240g),which were maintained for 16 days under controlled environment and supplied with water and Purina® rodent ad libitum. The animals were separated into four groups: CG - control group; G0,5 - dose of 0.5 ml / kg body weight (bw); G0,75 - dose of 0.75 ml / kg bw and G1 - dose of 1 ml / kg bw. CCl4 was administered by intraperitoneal injection twice a week. 24h after the last CCl4 administration, all animals were anesthetized (xylazine: ketamine - 1: 1 v / v) for performing cardiac puncture, euthanasia and dissection of the liver for removal and analysis. Results obtained were subjected to normality test, and followed by the analysis of variance (ANOVA) and comparison of means (Tukey at 5% probability - post-hoc). It was observed that the mean weights of rats treated with CCl4 were higher than that of to the GC and the liver protein content (in g / 100 g) was lower in the treated groups, with no statistical difference between the test groups. Histopathological analysis showed changes in the structure, increased numbers of macrophages with intracellular lipid accumulation and increased cellular infiltration in groups treated with CCl4. By quantifying the enzymes alanine aminotransferase (ALT), aspartate and alanine aminotransferase (AST), it was found that the CCl4-treated groups showed significantly higher levels than CG, being higher in G1 compared to the other groups tested. All tested concentrations of CCl4 induced liver injury in vivo, in different degrees, and the concentration of 0.5 mL / kg bw, administered twice weekly, was the lowest dose tested that could cause changes in all the parameters evaluated. Therefore, this is the ideal dose for induction of acute liver injury, aiming to test the modulatory role of dietary and nutritional factors.O Tetracloreto de Carbono (CCl4) é reconhecido como hepatotoxina clássica, sendo considerada a melhor substância para induzir lesão hepática, comumente utilizado como modelo para testar o efeito hepatoprotetor de drogas e substâncias naturais e para investigação de hepatotoxicidade, citotoxicidade e estresse oxidativo, tanto para estudos in vivo quanto in vitro. O presente estudo visou padronizar a menor dose de CCl4 capaz de provocar lesão hepática aguda por estresse oxidativo em ratos Wistar. O experimento in vivo foi realizado com 12 ratos Wistar adultos, machos, mantidos em condições de ambiente controladas e fornecimento de água e ração distribuídos ad libitum. O ensaio teve duração de 16 dias. Os animais foram separados em quatro grupos, sendo: GC – grupo controle; G0,5 – dose de 0,5 mL/kg de peso corporal (pc); G0,75 – dose de 0,75 mL/kg de pc e G1 – dose de 1 mL/kg de pc. O CCI4 foi administrado via injeção intraperitoneal, duas vezes por semana. Após 24h da última administração de CCI4, todos os animais foram anestesiados para realização de punção cardíaca, eutanásia e dissecação para retirada do fígado e análises. Os resultados obtidos foram submetidos ao teste de normalidade e, posteriormente, à análise de variância e comparação de médias. Observou-se que o peso do fígado dos ratos tratados com CCl4 foi maior em todos os grupos tratados em comparação ao GC e o teor protéico do fígado (em g/100g) foi menor nos grupos tratados, sem diferença estatística entre os grupos teste. As análises histopatológicas mostraram alteração na estrutura do tecido hepático, aumento do número de macrófagos com acúmulo intracelular de lipídeos e aumento do infiltrado celular nos grupos tratados com CCl4. Por meio da quantificação das enzimas alanina aminotransferase (ALT) e alanina aspartato aminotransferase (AST), verificou-se que os grupos tratados com CCl4 apresentaram concentrações significativamente mais elevadas em relação ao GC, sendo maiores no G1 em comparação aos demais grupos testados. Todas as concentrações de CCl4 testadas induziram lesão hepática, em diferentes graus, sendo a concentração de 0,5 mL/kg de pc, administrada duas vezes por semana, a menor dose testada que conseguiu provocar alterações em todos os parâmentos avaliados. Portanto, esta é a dose mais indicada para indução de lesão hepática aguda, objetivando testar o papel modulador de fatores alimentares e nutricionais.Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2015-03-17T11:16:45Z No. of bitstreams: 2 Dissertação - Barbara Martins Vieira - 2014.pdf: 1451459 bytes, checksum: 595a3718c8c4e83f9334cf6513f85119 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-03-20T14:04:37Z (GMT) No. of bitstreams: 2 Dissertação - Barbara Martins Vieira - 2014.pdf: 1451459 bytes, checksum: 595a3718c8c4e83f9334cf6513f85119 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2015-03-20T14:04:37Z (GMT). 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dc.title.por.fl_str_mv |
Padronização de dose de tetracloreto de carbono em modelo de lesão hepática aguda por estresse oxidativo em ratos Wistar |
dc.title.alternative.eng.fl_str_mv |
Carbon tetrachloride dose standardization of liver injury model acute oxidative stress in rats |
title |
Padronização de dose de tetracloreto de carbono em modelo de lesão hepática aguda por estresse oxidativo em ratos Wistar |
spellingShingle |
Padronização de dose de tetracloreto de carbono em modelo de lesão hepática aguda por estresse oxidativo em ratos Wistar Vieira, Bárbara Martins Fígado Modelo CCl4 Ratos Hepatotoxicidade Hepatotoxicity Liver Model Rats CIENCIAS DA SAUDE::NUTRICAO |
title_short |
Padronização de dose de tetracloreto de carbono em modelo de lesão hepática aguda por estresse oxidativo em ratos Wistar |
title_full |
Padronização de dose de tetracloreto de carbono em modelo de lesão hepática aguda por estresse oxidativo em ratos Wistar |
title_fullStr |
Padronização de dose de tetracloreto de carbono em modelo de lesão hepática aguda por estresse oxidativo em ratos Wistar |
title_full_unstemmed |
Padronização de dose de tetracloreto de carbono em modelo de lesão hepática aguda por estresse oxidativo em ratos Wistar |
title_sort |
Padronização de dose de tetracloreto de carbono em modelo de lesão hepática aguda por estresse oxidativo em ratos Wistar |
author |
Vieira, Bárbara Martins |
author_facet |
Vieira, Bárbara Martins |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Naves, Maria Margareth Veloso |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6563181057140270 |
dc.contributor.referee1.fl_str_mv |
Cominetti, Cristiane |
dc.contributor.referee2.fl_str_mv |
Horst, Maria Aderuza |
dc.contributor.referee3.fl_str_mv |
Naves, Maria Margareth Veloso |
dc.contributor.advisor2.fl_str_mv |
Celes, Mara Rúbia Nunes |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9945884989330825 |
dc.contributor.author.fl_str_mv |
Vieira, Bárbara Martins |
contributor_str_mv |
Naves, Maria Margareth Veloso Cominetti, Cristiane Horst, Maria Aderuza Naves, Maria Margareth Veloso Celes, Mara Rúbia Nunes |
dc.subject.por.fl_str_mv |
Fígado Modelo CCl4 Ratos Hepatotoxicidade Hepatotoxicity |
topic |
Fígado Modelo CCl4 Ratos Hepatotoxicidade Hepatotoxicity Liver Model Rats CIENCIAS DA SAUDE::NUTRICAO |
dc.subject.eng.fl_str_mv |
Liver Model Rats |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::NUTRICAO |
description |
The carbon tetrachloride (CCl4) is recognized as a classic hepatotoxin, being considered the best method to induce liver injury, commonly used as a model to test the hepatoprotective effect of drugs and natural substances and for investigation of hepatotoxicity, cytotoxicity and oxidative stress, in both in vivo and in vitro studies. This study aimed to standardize the lowest dose of CCl4 to cause acute liver injury by oxidative stress in Wistar rats. The in vivo experiment was carried out with 12 male Wistar rats (180-240g),which were maintained for 16 days under controlled environment and supplied with water and Purina® rodent ad libitum. The animals were separated into four groups: CG - control group; G0,5 - dose of 0.5 ml / kg body weight (bw); G0,75 - dose of 0.75 ml / kg bw and G1 - dose of 1 ml / kg bw. CCl4 was administered by intraperitoneal injection twice a week. 24h after the last CCl4 administration, all animals were anesthetized (xylazine: ketamine - 1: 1 v / v) for performing cardiac puncture, euthanasia and dissection of the liver for removal and analysis. Results obtained were subjected to normality test, and followed by the analysis of variance (ANOVA) and comparison of means (Tukey at 5% probability - post-hoc). It was observed that the mean weights of rats treated with CCl4 were higher than that of to the GC and the liver protein content (in g / 100 g) was lower in the treated groups, with no statistical difference between the test groups. Histopathological analysis showed changes in the structure, increased numbers of macrophages with intracellular lipid accumulation and increased cellular infiltration in groups treated with CCl4. By quantifying the enzymes alanine aminotransferase (ALT), aspartate and alanine aminotransferase (AST), it was found that the CCl4-treated groups showed significantly higher levels than CG, being higher in G1 compared to the other groups tested. All tested concentrations of CCl4 induced liver injury in vivo, in different degrees, and the concentration of 0.5 mL / kg bw, administered twice weekly, was the lowest dose tested that could cause changes in all the parameters evaluated. Therefore, this is the ideal dose for induction of acute liver injury, aiming to test the modulatory role of dietary and nutritional factors. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-08-22 |
dc.date.accessioned.fl_str_mv |
2015-03-20T14:04:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VIEIRA, B. M. Padronização de dose de tetracloreto de carbono em modelo de lesão hepática aguda por estresse oxidativo em ratos Wistar. 2014. 53 f. Dissertação ( Mestrado em Nutrição e Saúde) - Universidade Federal de Goiás, Goiânia, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/4309 |
dc.identifier.dark.fl_str_mv |
ark:/38995/0013000001srq |
identifier_str_mv |
VIEIRA, B. M. Padronização de dose de tetracloreto de carbono em modelo de lesão hepática aguda por estresse oxidativo em ratos Wistar. 2014. 53 f. Dissertação ( Mestrado em Nutrição e Saúde) - Universidade Federal de Goiás, Goiânia, 2014. ark:/38995/0013000001srq |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/4309 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-3010970718164250106 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
9028001981735587154 |
dc.relation.cnpq.fl_str_mv |
-1073001350029933100 |
dc.relation.sponsorship.fl_str_mv |
-2555911436985713659 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Nutrição e Saúde (FANUT) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Faculdade de Nutrição - FANUT (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
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UFG |
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UFG |
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Repositório Institucional da UFG |
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Repositório Institucional da UFG |
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Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
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tasesdissertacoes.bc@ufg.br |
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