Viabilidade de massas ovígeras de Biomphalaria glabrata em filme de água e susceptibilidade a Metarhizium anisopliae e Beauveria bassiana

• Autor(a) principal: Duarte, Glennyha Fernandes
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/4200
• topic: Pedra de argila; Ágar água; Blastosporos; Fungos entomopatogênicos; Clay stone; Water agar; Blastospores; Entomopathogenic fungi; CIENCIAS BIOLOGICAS::PARASITOLOGIA

Biomphalaria glabrata snails are aquatic and inhabit often ephemeral basins. They have high reproductive capacity, and egg masses are laid on submerged substrates on the banks of breeding sites exposed to desiccation and pathogens like fungi. Virtually nothing is known about the viability of egg masses in breeding and transient sites and their pathogens. This information is important to control this intermediate host of Schistosoma mansoni, which causes schistosomiasis. This study describes the feasibility of egg masses in water films and susceptibility infection with to Beauveria bassiana and Metarhizium ansiopliae. Egg masses were exposed to water film formed in two different devices, one with a stone clay in a cup with water and another one with water agar at different concentrations of agar (0.3% - 3%) in Petri dishes in different relative humidity (RH > 98% and 75%). Conidia or blastospores of B. bassiana (ARSEF 9588) or M. anisopliae (IP 46) (2 x 107 conidia or blastospores/egg mass) were inoculated in egg masses exposed in the water film in stone clay or submerged (2 x 107 conidia/ml or blastospores/ml). Egg masses exposed in the water film in stone clay or in water agar (agar < 1%) at RH > 98% were viable. The eclosion and the displacement of juveniles in the films were observed regardless of the tested device (RH > 98%, agar < 1%), except at 75% RH and glass without water film. Mycelium and conidia developed on egg masses treated with conidia or blastospores of M. anisopliae in the water film in stone clay (RH > 98%), and there was no eclosion of juveniles from egg masses treated with blastospores of M. anisopliae. In egg masses submerged treated with blastospores of M. anisopliae or B. bassiana no eclosion of juveniles and egg masses treated with conidia accumulated average onset was < 52.5%. The feasibility of egg masses and the displacement of juveniles in both devices on RH > 98% was due to the formation of stable water film. Blastospores may have released toxic metabolites that inhibited the eclosion of juvenile B. glabrata. Both devices are promising for testing egg masses in the water film. The feasibility of egg masses in water under laboratory conditions film should be considered to control B. glabrata. Blastospores of M. anisopliae and B. bassiana are promising against B. glabrata.