Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos

Detalhes bibliográficos
Autor(a) principal: Cunha, Carolina Cândida de Queiroz Brito
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
dARK ID: ark:/38995/001300000796w
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/3119
Resumo: An actinomycete strain, isolated from cane sugar bagasse (CSB), identified as Streptomyces sp was selected for its ability to produce cellulases. The production of cellulases was analyzed by submerged fermentation by cultivation on minimal medium (MM) containing CSB, wheat bran (WB) or carboxymethylcellulose (CMC) as carbon source, and yeast extract (YE) as nitrogen source. The results show that WB was the best inducer of CMCases (2.0 U.mL-1). Aiming to analyze the production of cellulases and xylanases kinetics, the isolate was inoculated in minimal medium containing 0.5% (w/v) WB and maintained for 12 days at 45°C under constant agitation of 180 rpm. The highest yield of Avicelase was observed after 264 h of cultivation (5.646 Uml-1), after 144 h for CMCase (3.872 Uml-1), after 144 h for FPase (0.0947 Uml-1) and after 288 h for Xylanase (92.40 Uml- 1). Culture supernatants with maximum activity of Avicelase, CMCase, Fpase and Xylanase were analyzed for optima pH and temperature of the respective enzymes. The highest enzyme activities were detected at pH 7.0 at 35°C for Avicelase, pH 4.5/75°C for CMCase, pH 5.5/45°Cfor FPase and pH 5.5/70°C for Xylanase. The enzymes retained more than 70% of the initial activity after 2 h incubation at 50°C. The profile proteins analyzed by zymogram demonstrated a set of secreted cellulases (37, 21 and 17 kDa) and xylanases (39, 21, 18 and 17 kDa) when grown on FT for 144 h. The saccharification assay with CSB as substrate showed that the enzyme complex was able to release 19% of glucose and 62.9% of xylose.
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spelling Bataus, Luiz Artur Mendeshttp://lattes.cnpq.br/3739169267521003http://lattes.cnpq.br/3556341716515132Cunha, Carolina Cândida de Queiroz Brito2014-09-22T19:09:30Z2012-10-27CUNHA, Carolina Cândida de Queiroz Brito. Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos. 2012. 99 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2012.http://repositorio.bc.ufg.br/tede/handle/tede/3119ark:/38995/001300000796wAn actinomycete strain, isolated from cane sugar bagasse (CSB), identified as Streptomyces sp was selected for its ability to produce cellulases. The production of cellulases was analyzed by submerged fermentation by cultivation on minimal medium (MM) containing CSB, wheat bran (WB) or carboxymethylcellulose (CMC) as carbon source, and yeast extract (YE) as nitrogen source. The results show that WB was the best inducer of CMCases (2.0 U.mL-1). Aiming to analyze the production of cellulases and xylanases kinetics, the isolate was inoculated in minimal medium containing 0.5% (w/v) WB and maintained for 12 days at 45°C under constant agitation of 180 rpm. The highest yield of Avicelase was observed after 264 h of cultivation (5.646 Uml-1), after 144 h for CMCase (3.872 Uml-1), after 144 h for FPase (0.0947 Uml-1) and after 288 h for Xylanase (92.40 Uml- 1). Culture supernatants with maximum activity of Avicelase, CMCase, Fpase and Xylanase were analyzed for optima pH and temperature of the respective enzymes. The highest enzyme activities were detected at pH 7.0 at 35°C for Avicelase, pH 4.5/75°C for CMCase, pH 5.5/45°Cfor FPase and pH 5.5/70°C for Xylanase. The enzymes retained more than 70% of the initial activity after 2 h incubation at 50°C. The profile proteins analyzed by zymogram demonstrated a set of secreted cellulases (37, 21 and 17 kDa) and xylanases (39, 21, 18 and 17 kDa) when grown on FT for 144 h. The saccharification assay with CSB as substrate showed that the enzyme complex was able to release 19% of glucose and 62.9% of xylose.Uma linhagem de Actinomiceto, isolada do bagaço de cana-de-açúcar (BCA), identificada como Streptomyces sp foi selecionada pela sua capacidade de produzir celulases. A produção de celulases foi analisada por fermentação submersa (FS) pelo cultivo do isolado em meio mínimo (MM) contendo BCA, farelo de trigo (FT) ou carboximetilcelulose (CMC) como fonte de carbono, e extrato de levedura (EL) como fonte de nitrogênio. Os resultados demostraram que o FT foi o melhor indutor da produção de CMCases (2,0 U.mL-1). Com o objetivo de analisar a cinética de produção de celulases e xilanases pelo isolado, este foi inoculado em meio mínimo contendo 0,5% (w/v) FT e mantido por 12 dias a 45°C sob agitação constante de 180 rpm. A maior produção de Avicelase foi observada após 264 h de cultivo (5,646 UmL-1), de CMCase após 144 h (3,872 UmL-1), de FPase após 144 h (0,0947 UmL-1) e de Xilanase após 288 h (92,40 UmL-1). Os sobrenantes de cultura com atividade máxima de Avicelase, CMCase, FPase e Xilanase foram analisados quanto ao pH e temperatura ótimos das respectivas enzimas. Os resultados obtidos demonstraram que a maior atividade de Avicelase foi detectada em pH 7,0 a 35°C; CMCase apresentou melhor atividade em pH 4,5 a 75°C; FPase apresentou melhor atividade em pH 5,5 a 45°C e Xilanase apresentou melhor atividade em pH 5,5 a 70°C. Quanto à termoestabilidade, as enzimas presentes mantiveram mais de 70% da atividade inicial após 2 h de incubação a 50°C. O perfil de proteínas analisado por zimograma demonstrou que o isolado secretou um conjunto de celulases (37, 21 e 17 KDa) e xilanases (39, 21, 18 e 17 KDa) quando cultivado em FT por 144 h. No ensaio de sacarificação de BCA o complexo enzimático foi capaz de liberar 19% de glicose e 62,9% de xilose.Submitted by Erika Demachki (erikademachki@gmail.com) on 2014-09-18T19:15:37Z No. of bitstreams: 2 Carolina C. de Queiroz Brito Cunha - 2012.pdf: 4678972 bytes, checksum: aa3da530028732bcbc87f49ba9ea6725 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Rejected by Luciana Ferreira (lucgeral@gmail.com), reason: Há problemas nos campos de palavras chaves e citação. Foi acrescentado da seguinte forma: Streptomyces - bagaço de cana. De acordo com as orientações seria: Streptomyces Bagaço de cana Foi acrescentado no campo de citação: Citação: Cunha, Carolina Cândida de Queiroz Brito - Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos - 2012 - 99 f. - Dissertação - Programa de Pós-graduação em Biologia (ICB) - Universidade Federal de Goiás - Goiânia, 2012. Deve-se usar a NBR6023, ex.: ALCÂNTARA, Guizelle Aparecida de. Caracterização farmacognostica e atividade antimicrobiana da folha e casca do caule da myrciarostratadc.(myrtaceae). 2012. 41 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2012. on 2014-09-19T13:12:18Z (GMT)Submitted by Erika Demachki (erikademachki@gmail.com) on 2014-09-22T18:04:37Z No. of bitstreams: 2 Carolina C. de Queiroz Brito Cunha - 2012.pdf: 4678972 bytes, checksum: aa3da530028732bcbc87f49ba9ea6725 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Jaqueline Silva (jtas29@gmail.com) on 2014-09-22T19:09:30Z (GMT) No. of bitstreams: 2 Carolina C. de Queiroz Brito Cunha - 2012.pdf: 4678972 bytes, checksum: aa3da530028732bcbc87f49ba9ea6725 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2014-09-22T19:09:30Z (GMT). No. of bitstreams: 2 Carolina C. de Queiroz Brito Cunha - 2012.pdf: 4678972 bytes, checksum: aa3da530028732bcbc87f49ba9ea6725 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2012-10-27Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfhttp://repositorio.bc.ufg.br/tede/retrieve/8215/Carolina%20C.%20de%20Queiroz%20%20Brito%20Cunha%20-%202012.pdf.jpgporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)ADNEY, B.; BAKER, J. Measurement of cellulase activities. Chemical Analysis and Testing Task: Laboratory Analytical Procedure. Golden, CO: National Renewable Energy Laboratory, 11 p. (NREL LAP, 006). ALANI, F., ANDERSON, W.A., YOUNG, M.M. (2008) New isolate of Streptomyces sp. With novel thermoalkalotolerant cellulases. Biotechnol Lett 30: 123-126 ANTANOPOULUS, V.T.; HERNANDEZ, M; ARIAS, M.E.; MAVRAKOS, E. 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dc.title.por.fl_str_mv Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos
dc.title.alternative.eng.fl_str_mv Production and characterization of cellulases and xylanases by thermophilic Streptomyces sp. grown on lignocellulosic wastes
title Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos
spellingShingle Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos
Cunha, Carolina Cândida de Queiroz Brito
Streptomyces - bagaço de cana
Streptomyces - celulase
cellulases and xylanases
MICROBIOLOGIA::MICROBIOLOGIA APLICADA
title_short Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos
title_full Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos
title_fullStr Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos
title_full_unstemmed Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos
title_sort Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos
author Cunha, Carolina Cândida de Queiroz Brito
author_facet Cunha, Carolina Cândida de Queiroz Brito
author_role author
dc.contributor.advisor1.fl_str_mv Bataus, Luiz Artur Mendes
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3739169267521003
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3556341716515132
dc.contributor.author.fl_str_mv Cunha, Carolina Cândida de Queiroz Brito
contributor_str_mv Bataus, Luiz Artur Mendes
dc.subject.por.fl_str_mv Streptomyces - bagaço de cana
Streptomyces - celulase
topic Streptomyces - bagaço de cana
Streptomyces - celulase
cellulases and xylanases
MICROBIOLOGIA::MICROBIOLOGIA APLICADA
dc.subject.eng.fl_str_mv cellulases and xylanases
dc.subject.cnpq.fl_str_mv MICROBIOLOGIA::MICROBIOLOGIA APLICADA
description An actinomycete strain, isolated from cane sugar bagasse (CSB), identified as Streptomyces sp was selected for its ability to produce cellulases. The production of cellulases was analyzed by submerged fermentation by cultivation on minimal medium (MM) containing CSB, wheat bran (WB) or carboxymethylcellulose (CMC) as carbon source, and yeast extract (YE) as nitrogen source. The results show that WB was the best inducer of CMCases (2.0 U.mL-1). Aiming to analyze the production of cellulases and xylanases kinetics, the isolate was inoculated in minimal medium containing 0.5% (w/v) WB and maintained for 12 days at 45°C under constant agitation of 180 rpm. The highest yield of Avicelase was observed after 264 h of cultivation (5.646 Uml-1), after 144 h for CMCase (3.872 Uml-1), after 144 h for FPase (0.0947 Uml-1) and after 288 h for Xylanase (92.40 Uml- 1). Culture supernatants with maximum activity of Avicelase, CMCase, Fpase and Xylanase were analyzed for optima pH and temperature of the respective enzymes. The highest enzyme activities were detected at pH 7.0 at 35°C for Avicelase, pH 4.5/75°C for CMCase, pH 5.5/45°Cfor FPase and pH 5.5/70°C for Xylanase. The enzymes retained more than 70% of the initial activity after 2 h incubation at 50°C. The profile proteins analyzed by zymogram demonstrated a set of secreted cellulases (37, 21 and 17 kDa) and xylanases (39, 21, 18 and 17 kDa) when grown on FT for 144 h. The saccharification assay with CSB as substrate showed that the enzyme complex was able to release 19% of glucose and 62.9% of xylose.
publishDate 2012
dc.date.issued.fl_str_mv 2012-10-27
dc.date.accessioned.fl_str_mv 2014-09-22T19:09:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv CUNHA, Carolina Cândida de Queiroz Brito. Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos. 2012. 99 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2012.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/3119
dc.identifier.dark.fl_str_mv ark:/38995/001300000796w
identifier_str_mv CUNHA, Carolina Cândida de Queiroz Brito. Caracterização de celulases e xilanases produzidas por Streptomyces sp. cultivado em resíduos lignocelulósicos. 2012. 99 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2012.
ark:/38995/001300000796w
url http://repositorio.bc.ufg.br/tede/handle/tede/3119
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 6883982777473437920
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv -3872772117827373404
dc.relation.cnpq.fl_str_mv -203884118251241698
dc.relation.sponsorship.fl_str_mv -2555911436985713659
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