Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/5759 |
Resumo: | The main transmitter species of serotypes of the dengue virus is Aedes aegypti, the most competent mosquitoes and more adapted to urban and man-made environment. The control measures of this disease are still mainly on the vector. Recently, several studies have shown the use of botanical origin of substances with potential insecticide in control of Ae. aegypti, alternatively opposite resistance to conventional insecticides. The efficacy oleoresin of C. langsdorffii the control of Ae. aegypti demonstrated in field work, signals the promising possibility of this botanical compound to control this vector requiring studies to clarify the mechanism of action. Thus, it is proposed to establish the mechanism of death process in the third stage larvae of Ae. aegypti through morfohistologic studies. The larvae of Ae. aegypti were subjected to oil-resin solutions and hexane fraction C. langsdorffii in concentrations of 70 ppm and 80 ppm, respectively. Subsequently collected in time intervals of 6h, 12h and 24h. They were fixed in 4% paraformaldhyde in 0.1 M sodium phosphate buffer pH 7.2, embedded in resin, mounted, stained with hematoxylin-eosin and analyzed by light microscopy. The oil-resin and hexane fraction C. langsdorffii, caused the death of the larvae by the destruction of the posterior cells of the midgut region through cytoplasmatic vacuolation, apical cytoplasmic vesicle formation, lip brush degeneration, increased cellular volume and fold in peritrophic matrix. The changes described in this paper demonstrate how bioactive compounds can trigger cell degeneration and accelerate cell disruption. |
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Silva, Ionizete Garcia dahttp://lattes.cnpq.br/5021551669347602Arruda, WalquíriaSilva, Heloisa Helena Garcia daSilva, Ionizete Garcia dahttp://lattes.cnpq.br/3563091265193700Santos, Daniela Braz dos2016-07-29T21:23:15Z2015-06-30SANTOS, D. B. Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae). 2015. 71 f. Dissertação (mestrado em Biologia das Interações Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/5759The main transmitter species of serotypes of the dengue virus is Aedes aegypti, the most competent mosquitoes and more adapted to urban and man-made environment. The control measures of this disease are still mainly on the vector. Recently, several studies have shown the use of botanical origin of substances with potential insecticide in control of Ae. aegypti, alternatively opposite resistance to conventional insecticides. The efficacy oleoresin of C. langsdorffii the control of Ae. aegypti demonstrated in field work, signals the promising possibility of this botanical compound to control this vector requiring studies to clarify the mechanism of action. Thus, it is proposed to establish the mechanism of death process in the third stage larvae of Ae. aegypti through morfohistologic studies. The larvae of Ae. aegypti were subjected to oil-resin solutions and hexane fraction C. langsdorffii in concentrations of 70 ppm and 80 ppm, respectively. Subsequently collected in time intervals of 6h, 12h and 24h. They were fixed in 4% paraformaldhyde in 0.1 M sodium phosphate buffer pH 7.2, embedded in resin, mounted, stained with hematoxylin-eosin and analyzed by light microscopy. The oil-resin and hexane fraction C. langsdorffii, caused the death of the larvae by the destruction of the posterior cells of the midgut region through cytoplasmatic vacuolation, apical cytoplasmic vesicle formation, lip brush degeneration, increased cellular volume and fold in peritrophic matrix. The changes described in this paper demonstrate how bioactive compounds can trigger cell degeneration and accelerate cell disruption.A principal espécie transmissora dos sorotipos do vírus dengue é o Aedes aegypti, o mosquito mais competente e mais adaptado ao ambiente urbano e antrópico. As medidas de controle dessa doença ainda estão prioritariamente sobre o vetor. Recentemente, diversos estudos têm evidenciado o uso de substâncias de origem botânica com potencial inseticida no controle de Ae. aegypti, como alternativa frente à resistência aos inseticidas convencionais. A eficácia do óleo-resina de Copaifera langsdorffii no controle das larvas de Ae. aegypti demonstrada em trabalhos de campo, sinaliza a possibilidade promissora desse composto botânico para o controle desse vetor requerendo estudos que esclareçam o seu mecanismo de ação. Dessa forma, propõe-se demonstrar o mecanismo do processo de mortalidade em larvas de terceiro estádio de Ae. aegypti, através de estudos morfohistológicos. As larvas de Ae. aegypti foram submetidas às soluções de óleo-resina e fração hexânica de C. langsdorffii nas concentrações de 70 ppm e 80 ppm, respectivamente. Posteriormente coletadas nos intervalos de tempo de 6 horas, 12horas e 24horas. Foram fixadas em paraformaldeído a 4% com tampão fosfato de sódio 0,1 M pH 7,2, incluídas em resina, montadas, coradas pela hematoxilina-eosina e analisadas por microscopia de luz. O óleo-resina e fração hexânica de C. langsdorffii, causaram a morte das larvas pela destruição das células da região posterior do mesêntero, por meio de intensa vacuolização citoplasmática, formação de vesícula citoplasmática apical, degeneração do bordo em escova, aumento do volume celular e a dobra na matriz peritrófica. As alterações descritas neste trabalho demonstram como compostos bioativos desencadeiam a degeneração celular e aceleram o rompimento celular.Submitted by Erika Demachki (erikademachki@gmail.com) on 2016-07-29T21:21:54Z No. of bitstreams: 2 Dissertação - Daniela Braz dos Santos - 2015.pdf: 4725212 bytes, checksum: 2e490fbb0143d4fa27287f47ec72f3eb (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Erika Demachki (erikademachki@gmail.com) on 2016-07-29T21:23:15Z (GMT) No. of bitstreams: 2 Dissertação - Daniela Braz dos Santos - 2015.pdf: 4725212 bytes, checksum: 2e490fbb0143d4fa27287f47ec72f3eb (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2016-07-29T21:23:15Z (GMT). No. of bitstreams: 2 Dissertação - Daniela Braz dos Santos - 2015.pdf: 4725212 bytes, checksum: 2e490fbb0143d4fa27287f47ec72f3eb (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-06-30application/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia das Interações PH (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAedes aegyptiCopaifera langsdorffiiAtividade larvicidaAlteração morfohistológicaLarvicidal activityMorpho-histologicalCLINICA MEDICA::DOENCAS INFECCIOSAS E PARASITARIASAtividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae)Larvicidal activity Copaifera langsdorffii (Fabaceae), evidenced by morphological histological changes in Aedes aegypti (Diptera, Culicidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6367159433410056917600600600-77690114445645562881767748423488408711reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae) |
dc.title.alternative.eng.fl_str_mv |
Larvicidal activity Copaifera langsdorffii (Fabaceae), evidenced by morphological histological changes in Aedes aegypti (Diptera, Culicidae) |
title |
Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae) |
spellingShingle |
Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae) Santos, Daniela Braz dos Aedes aegypti Copaifera langsdorffii Atividade larvicida Alteração morfohistológica Larvicidal activity Morpho-histological CLINICA MEDICA::DOENCAS INFECCIOSAS E PARASITARIAS |
title_short |
Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae) |
title_full |
Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae) |
title_fullStr |
Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae) |
title_full_unstemmed |
Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae) |
title_sort |
Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae) |
author |
Santos, Daniela Braz dos |
author_facet |
Santos, Daniela Braz dos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Ionizete Garcia da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5021551669347602 |
dc.contributor.referee1.fl_str_mv |
Arruda, Walquíria |
dc.contributor.referee2.fl_str_mv |
Silva, Heloisa Helena Garcia da |
dc.contributor.referee3.fl_str_mv |
Silva, Ionizete Garcia da |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3563091265193700 |
dc.contributor.author.fl_str_mv |
Santos, Daniela Braz dos |
contributor_str_mv |
Silva, Ionizete Garcia da Arruda, Walquíria Silva, Heloisa Helena Garcia da Silva, Ionizete Garcia da |
dc.subject.por.fl_str_mv |
Aedes aegypti Copaifera langsdorffii Atividade larvicida Alteração morfohistológica |
topic |
Aedes aegypti Copaifera langsdorffii Atividade larvicida Alteração morfohistológica Larvicidal activity Morpho-histological CLINICA MEDICA::DOENCAS INFECCIOSAS E PARASITARIAS |
dc.subject.eng.fl_str_mv |
Larvicidal activity Morpho-histological |
dc.subject.cnpq.fl_str_mv |
CLINICA MEDICA::DOENCAS INFECCIOSAS E PARASITARIAS |
description |
The main transmitter species of serotypes of the dengue virus is Aedes aegypti, the most competent mosquitoes and more adapted to urban and man-made environment. The control measures of this disease are still mainly on the vector. Recently, several studies have shown the use of botanical origin of substances with potential insecticide in control of Ae. aegypti, alternatively opposite resistance to conventional insecticides. The efficacy oleoresin of C. langsdorffii the control of Ae. aegypti demonstrated in field work, signals the promising possibility of this botanical compound to control this vector requiring studies to clarify the mechanism of action. Thus, it is proposed to establish the mechanism of death process in the third stage larvae of Ae. aegypti through morfohistologic studies. The larvae of Ae. aegypti were subjected to oil-resin solutions and hexane fraction C. langsdorffii in concentrations of 70 ppm and 80 ppm, respectively. Subsequently collected in time intervals of 6h, 12h and 24h. They were fixed in 4% paraformaldhyde in 0.1 M sodium phosphate buffer pH 7.2, embedded in resin, mounted, stained with hematoxylin-eosin and analyzed by light microscopy. The oil-resin and hexane fraction C. langsdorffii, caused the death of the larvae by the destruction of the posterior cells of the midgut region through cytoplasmatic vacuolation, apical cytoplasmic vesicle formation, lip brush degeneration, increased cellular volume and fold in peritrophic matrix. The changes described in this paper demonstrate how bioactive compounds can trigger cell degeneration and accelerate cell disruption. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-06-30 |
dc.date.accessioned.fl_str_mv |
2016-07-29T21:23:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, D. B. Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae). 2015. 71 f. Dissertação (mestrado em Biologia das Interações Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/5759 |
identifier_str_mv |
SANTOS, D. B. Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae). 2015. 71 f. Dissertação (mestrado em Biologia das Interações Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/5759 |
dc.language.iso.fl_str_mv |
por |
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por |
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6367159433410056917 |
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600 600 600 |
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dc.relation.cnpq.fl_str_mv |
1767748423488408711 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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Universidade Federal de Goiás |
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Programa de Pós-graduação em Biologia das Interações PH (IPTSP) |
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UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
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