Sensores eletroanalíticos de sonogel e pasta de carbono para análise de paracetamol gotas

• Autor(a) principal: Crispim, Denise Vaz Ferreira da Silva
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/5077
• topic: Eletrodos modificados; Desenvolvimento farmacêutico; Formas de dosagem; Controle de qualidade; Sonogel-carbon electrodes; Pharmaceutical development; Dosage forms; Quality control; CIENCIAS DA SAUDE::FARMACIA

Paracetamol (N-acetyl-p-aminophenol or acetaminophen) is a drug with antipyretic, analgesic and anti-inflammatory, stands out as one of the most consumed worldwide. Because of this demand, the development and registration of new formulations by the pharmaceutical sector is ongoing. The process control and final product is an obligatory part of quality control. The official methods for quantitative determination of paracetamol include spectrophotometric and chromatographic techniques. In turn, due to the inherent electroactivity of this compound, the scientific literature involving electroanalytical methods is also large. Our objective was to demonstrate the efficiency of carbon electrodes modified with silica sonogel compared to conventional electrodes and official methods. The development and validation of an electroanalytical method for quantitative determination of paracetamol in liquid formulations. Apply the method in different product development phases, like product and reference, comparing the method developed with the official method of CLAE using conventional glassy carbon electrodes and carbon and sonogel folder. The samples consisted of a formulation drops reference (Tylenol®), 5 test formulations, standard solution of Paracetamol, all the 200 mg/mL. For differential pulse voltammetry (DPV) was used a potentiostat/galvanostat. Electrochemical cell with 5.0mL capacity, with three-electrode system, glassy carbon electrodes or sonogel, Pt ring and calomel (SCE), represented the electrodes working, auxiliary and reference, respectively. The VPD conditions were: 75mV pulse amplitude, pulse width of 0.4 is scanning speed of 5 mV/s. For chromatographic testing was performed using a UV detector at 272 nm, C18 column (200 mm x 4.6 mm x 10 microns) with a mobile phase flow 2 ml/min. The observed voltammetric profiles showed an anodic peak at 293 mV (vs. Ag/AgClsat, pH = 6.0), whose potential and current levels was lower than that observed for cabono of electrode. Have excipients showed no do not constitute interfering peaks. Carbon electrodes with nanostructured silica sonogel also showed good linearity and recovery rate (CV <5%) compared to standard solution and official method (CLAE). This fact combined with good selectivity, sensitivity, and low overall cost makes this very promising device for analysis of paracetamol formulations.