Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/12522 |
Resumo: | Rural communities are located far from urban centers, where the adequate supply of potable water is hampered. In the lack of a public distribution network, water is obtained from surface and groundwater sources, where the microbial quality is often unknown. Goiás has 9.7% of the population in rural areas and previous studies carried out in some of these rural and traditional communities have already demonstrated the vulnerability of water for the consumption of these residents by waterborne pathogens, such as enteric viruses. Therefore, the aim of the work was to continue the evaluation of fecal contamination of water samples from individual and collective sources, from 24 communities in rural areas of the State of Goiás. 160 samples were collected, whose water sources were shallow tubular well, deep tubular well, dug well and spring, surface water, rainwater stored in cisterns and water truck. The enteric viruses Human Adenovirus (HAdV), Rotavirus (RV) and Enterovirus (EV) were used as indicators of fecal contamination, analyzed by qPCR. In all, 30% of the samples showed signs of fecal contamination. Detection rates were 20% for RV, 9.4% for HAdV and 4.4% for EV. The geometric means of concentration of these indicators were 1.5x106 GC/L, 1.9x106 GC/L and 7.1x105 GC/L, respectively. RV was the most prevalent viral indicator in underground sources, being statistically associated with shallow tubular wells. In spring samples, HAdV stands out with 11.8%. In the cistern samples, both HAdV and RV were found in the same prevalence. These results reveals that the analyzed population is vulnerable to waterborne diseases caused by enteric pathogens, serving to guide future decisions and improve daily water treatment practices in these communities. |
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Carneiro, Lilian Carlahttp://lattes.cnpq.br/6506744224041777Carneiro, Lilian CarlaAlbuquerque, Antônio João Carvalho deOliveira, Bruno Francesco Rodrigues dehttp://lattes.cnpq.br/0753409922612066Bordoni, Graziela Picciola2022-12-26T13:27:08Z2022-12-26T13:27:08Z2022-11-28BORDONI, G. P. Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás. 2022. 113 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2022.http://repositorio.bc.ufg.br/tede/handle/tede/12522Rural communities are located far from urban centers, where the adequate supply of potable water is hampered. In the lack of a public distribution network, water is obtained from surface and groundwater sources, where the microbial quality is often unknown. Goiás has 9.7% of the population in rural areas and previous studies carried out in some of these rural and traditional communities have already demonstrated the vulnerability of water for the consumption of these residents by waterborne pathogens, such as enteric viruses. Therefore, the aim of the work was to continue the evaluation of fecal contamination of water samples from individual and collective sources, from 24 communities in rural areas of the State of Goiás. 160 samples were collected, whose water sources were shallow tubular well, deep tubular well, dug well and spring, surface water, rainwater stored in cisterns and water truck. The enteric viruses Human Adenovirus (HAdV), Rotavirus (RV) and Enterovirus (EV) were used as indicators of fecal contamination, analyzed by qPCR. In all, 30% of the samples showed signs of fecal contamination. Detection rates were 20% for RV, 9.4% for HAdV and 4.4% for EV. The geometric means of concentration of these indicators were 1.5x106 GC/L, 1.9x106 GC/L and 7.1x105 GC/L, respectively. RV was the most prevalent viral indicator in underground sources, being statistically associated with shallow tubular wells. In spring samples, HAdV stands out with 11.8%. In the cistern samples, both HAdV and RV were found in the same prevalence. These results reveals that the analyzed population is vulnerable to waterborne diseases caused by enteric pathogens, serving to guide future decisions and improve daily water treatment practices in these communities.As comunidades rurais estão localizadas longe dos centros urbanos, onde o fornecimento adequado de água potável é prejudicado. Na falta de uma rede pública de distribuição, a obtenção de água, é feita a partir de fontes superficiais e subterrâneas, onde a qualidade microbiana é muitas vezes desconhecida. Goiás possui 9,7% da população em áreas rurais e estudos anteriores feitos em algumas dessas comunidades rurais e tradicionais já demonstraram vulnerabilidade da água para o consumo desses residentes por patógenos de veiculação hídrica, como os vírus entéricos. Logo, o objetivo do trabalho foi continuar a avaliação da contaminação fecal de amostras de água provenientes de fontes individuais e coletivas, de 24 comunidades em áreas rurais do Estado de Goiás. Foram coletadas 160 amostras, cujas fontes de água eram poço tubular raso, poço tubular profundo, poço raso escavado e nascente, mananciais superficiais, água da chuva armazenada em cisternas e caminhão pipa. Utilizou-se como indicadores de contaminação fecal os vírus entéricos Adenovírus humano (HAdV), Rotavírus (RV) e Enterovírus (EV), analisados por qPCR. Ao todo, 30% das amostras apresentaram indicativo de contaminação fecal. As taxas de detecção foram de 20% para RV, 9,4% para HAdV e 4,4% para EV. As médias geométricas de concentração desses indicadores foram 1,5x106 CG/L, 1,9x106 CG/L e 7,1x105 CG/L, respectivamente. RV foi o indicador viral de maior prevalência em fontes subterrâneas, sendo estatisticamente associado a poços tubulares rasos. Em amostras de nascente, destaca-se o HAdV com 11,8%. Nas amostras de cisterna, tanto HAdV quanto RV, foram encontrados na mesma prevalência. Esses resultados revelam que a população analisada está vulnerável a doenças de veiculação hídrica causadas por patógenos entéricos, servindo para orientar a futuras decisões e aprimorar as práticas cotidianas de tratamento de água nestas comunidades.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2022-12-19T21:25:47Z No. of bitstreams: 2 Dissertação - Graziela Picciola Bordoni - 2022.pdf: 3521034 bytes, checksum: 56babd1633497e7ea51375bec1c43d95 (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2022-12-26T13:27:08Z (GMT) No. of bitstreams: 2 Dissertação - Graziela Picciola Bordoni - 2022.pdf: 3521034 bytes, checksum: 56babd1633497e7ea51375bec1c43d95 (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Made available in DSpace on 2022-12-26T13:27:08Z (GMT). No. of bitstreams: 2 Dissertação - Graziela Picciola Bordoni - 2022.pdf: 3521034 bytes, checksum: 56babd1633497e7ea51375bec1c43d95 (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Previous issue date: 2022-11-28OutroporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia da Relação Parasito-Hospedeiro (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RMG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessComunidades ruraisContaminação fecalFontes de águaVírus entéricosRural communitiesFecal contaminationWater sourcesEnteric virusesCIENCIAS BIOLOGICASIndicadores de contaminação viral em amostras de água consumida em regiões rurais de GoiásIndicators of viral contamination in water samples consumed in rural regions of Goiásinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis16500500500500281725reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/2ce878e6-3575-45b9-bb35-454829225a61/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/c0fef05b-7a67-4553-8190-36e4ecb9988a/download4460e5956bc1d1639be9ae6146a50347MD52ORIGINALDissertação - Graziela Picciola Bordoni - 2022.pdfDissertação - Graziela Picciola Bordoni - 2022.pdfapplication/pdf3521034http://repositorio.bc.ufg.br/tede/bitstreams/df2d5af5-83bd-4c10-81c5-1f8e57c8477d/download56babd1633497e7ea51375bec1c43d95MD53tede/125222022-12-26 10:27:08.261http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/12522http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2022-12-26T13:27:08Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.pt_BR.fl_str_mv |
Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás |
dc.title.alternative.eng.fl_str_mv |
Indicators of viral contamination in water samples consumed in rural regions of Goiás |
title |
Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás |
spellingShingle |
Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás Bordoni, Graziela Picciola Comunidades rurais Contaminação fecal Fontes de água Vírus entéricos Rural communities Fecal contamination Water sources Enteric viruses CIENCIAS BIOLOGICAS |
title_short |
Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás |
title_full |
Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás |
title_fullStr |
Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás |
title_full_unstemmed |
Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás |
title_sort |
Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás |
author |
Bordoni, Graziela Picciola |
author_facet |
Bordoni, Graziela Picciola |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Carneiro, Lilian Carla |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6506744224041777 |
dc.contributor.referee1.fl_str_mv |
Carneiro, Lilian Carla |
dc.contributor.referee2.fl_str_mv |
Albuquerque, Antônio João Carvalho de |
dc.contributor.referee3.fl_str_mv |
Oliveira, Bruno Francesco Rodrigues de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0753409922612066 |
dc.contributor.author.fl_str_mv |
Bordoni, Graziela Picciola |
contributor_str_mv |
Carneiro, Lilian Carla Carneiro, Lilian Carla Albuquerque, Antônio João Carvalho de Oliveira, Bruno Francesco Rodrigues de |
dc.subject.por.fl_str_mv |
Comunidades rurais Contaminação fecal Fontes de água Vírus entéricos |
topic |
Comunidades rurais Contaminação fecal Fontes de água Vírus entéricos Rural communities Fecal contamination Water sources Enteric viruses CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Rural communities Fecal contamination Water sources Enteric viruses |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS |
description |
Rural communities are located far from urban centers, where the adequate supply of potable water is hampered. In the lack of a public distribution network, water is obtained from surface and groundwater sources, where the microbial quality is often unknown. Goiás has 9.7% of the population in rural areas and previous studies carried out in some of these rural and traditional communities have already demonstrated the vulnerability of water for the consumption of these residents by waterborne pathogens, such as enteric viruses. Therefore, the aim of the work was to continue the evaluation of fecal contamination of water samples from individual and collective sources, from 24 communities in rural areas of the State of Goiás. 160 samples were collected, whose water sources were shallow tubular well, deep tubular well, dug well and spring, surface water, rainwater stored in cisterns and water truck. The enteric viruses Human Adenovirus (HAdV), Rotavirus (RV) and Enterovirus (EV) were used as indicators of fecal contamination, analyzed by qPCR. In all, 30% of the samples showed signs of fecal contamination. Detection rates were 20% for RV, 9.4% for HAdV and 4.4% for EV. The geometric means of concentration of these indicators were 1.5x106 GC/L, 1.9x106 GC/L and 7.1x105 GC/L, respectively. RV was the most prevalent viral indicator in underground sources, being statistically associated with shallow tubular wells. In spring samples, HAdV stands out with 11.8%. In the cistern samples, both HAdV and RV were found in the same prevalence. These results reveals that the analyzed population is vulnerable to waterborne diseases caused by enteric pathogens, serving to guide future decisions and improve daily water treatment practices in these communities. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-12-26T13:27:08Z |
dc.date.available.fl_str_mv |
2022-12-26T13:27:08Z |
dc.date.issued.fl_str_mv |
2022-11-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BORDONI, G. P. Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás. 2022. 113 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2022. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/12522 |
identifier_str_mv |
BORDONI, G. P. Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás. 2022. 113 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2022. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/12522 |
dc.language.iso.fl_str_mv |
por |
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por |
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500 500 500 500 |
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28 |
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172 |
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5 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Biologia da Relação Parasito-Hospedeiro (IPTSP) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RMG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
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