Identificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênita
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/11033 |
Resumo: | Introduction: The diagnosis of congenital toxoplasmosis is complex and might take months to confirm since most newborns do not present symptoms and levels of undetected IgM and IgA anti-Toxoplasma gondii antibodies. Besides, pregnant women may have residual IgM, making it difficult to diagnose acute infection during pregnancy. Despite the various serological tests developed, there are few studies about biomarkers that can assist in the diagnosis of acute and congenital toxoplasmosis. Objective: Analyze the immunoreactivity profile of T. gondii protein with the potential to be biomarkers for the diagnosis of acute toxoplasmosis and the diagnosis and prognosis of congenital toxoplasmosis. Methods: Serum samples were selected from children with symptomatic and asymptomatic congenital toxoplasmosis and women of childbearing age or pregnant women with acute and chronic acquired toxoplasmosis. These samples were grouped according to the patients' laboratory and clinical results. Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) was used to separate T. gondii proteins. The Immunoblotting technique was applied to the analysis of immunoreactive bands profile by IgG antibodies, using nitrocellulose membranes sensitized with parasite proteins. Results: One hundred and sixteen samples were analyzed. When comparing immunoreactive bands profile for antibodies present in samples from different groups and subgroups, the 150, 18.5, and 16.96 kDa proteins were more immunoreactive by the antibodies present in serum samples from the acquired infection group. The 343, 189, 150, 75, and 42 kDa proteins showed more chance to be detected by the symptomatic congenital infection subgroup, while the 61, 50, and 16.96 kDa proteins were significantly immunoreactive by the acute infection subgroup. Conclusions: Before obtained results, it was possible to identify potential biomarkers that may assist in early diagnosis and treatment of toxoplasmosis, avoiding the appearance of clinical manifestations throughout the life of children congenitally infected. |
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Castro, Ana Maria dehttp://lattes.cnpq.br/9232309971000621Borges, Clayton LuizAlves, Daniella de Sousa Mendes MoreiraRezende, Hanstter Hallison AlvesPaccez, Juliano DomiraciCastro, Ana Maria dehttp://lattes.cnpq.br/3543813852521316Storchilo, Heloisa Ribeiro2021-01-15T12:00:19Z2021-01-15T12:00:19Z2020-11-27STORCHILO, H. R. Identificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênita. 2020. 110 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2020.http://repositorio.bc.ufg.br/tede/handle/tede/11033ark:/38995/001300000dsdkIntroduction: The diagnosis of congenital toxoplasmosis is complex and might take months to confirm since most newborns do not present symptoms and levels of undetected IgM and IgA anti-Toxoplasma gondii antibodies. Besides, pregnant women may have residual IgM, making it difficult to diagnose acute infection during pregnancy. Despite the various serological tests developed, there are few studies about biomarkers that can assist in the diagnosis of acute and congenital toxoplasmosis. Objective: Analyze the immunoreactivity profile of T. gondii protein with the potential to be biomarkers for the diagnosis of acute toxoplasmosis and the diagnosis and prognosis of congenital toxoplasmosis. Methods: Serum samples were selected from children with symptomatic and asymptomatic congenital toxoplasmosis and women of childbearing age or pregnant women with acute and chronic acquired toxoplasmosis. These samples were grouped according to the patients' laboratory and clinical results. Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) was used to separate T. gondii proteins. The Immunoblotting technique was applied to the analysis of immunoreactive bands profile by IgG antibodies, using nitrocellulose membranes sensitized with parasite proteins. Results: One hundred and sixteen samples were analyzed. When comparing immunoreactive bands profile for antibodies present in samples from different groups and subgroups, the 150, 18.5, and 16.96 kDa proteins were more immunoreactive by the antibodies present in serum samples from the acquired infection group. The 343, 189, 150, 75, and 42 kDa proteins showed more chance to be detected by the symptomatic congenital infection subgroup, while the 61, 50, and 16.96 kDa proteins were significantly immunoreactive by the acute infection subgroup. Conclusions: Before obtained results, it was possible to identify potential biomarkers that may assist in early diagnosis and treatment of toxoplasmosis, avoiding the appearance of clinical manifestations throughout the life of children congenitally infected.O diagnóstico da toxoplasmose congênita é complexo e pode levar meses para ser confirmado, visto que a maioria dos recém-nascidos não apresenta sintomatologia e níveis detectáveis de anticorpos IgM e IgA anti-Toxoplasma gondii. Além disso, gestantes podem apresentar IgM residual, dificultando o diagnóstico da infecção aguda durante a gestão. Apesar dos diversos testes sorológicos desenvolvidos, há escassez na literatura sobre biomarcadores que possam auxiliar no diagnóstico precoce da toxoplasmose congênita e aguda. Objetivo: Analisar o perfil de imunoreatividade das proteínas de T. gondii com potencial para serem biomarcadores para o diagnóstico da toxoplasmose aguda e para o diagnóstico e prognóstico da toxoplasmose congênita. Métodos: Foram selecionadas amostras de soro de crianças com toxoplasmose congênita sintomática e assintomática e de mulheres em idade fértil e/ou gestantes com toxoplasmose adquirida aguda e crônica. Estas amostras foram agrupadas de acordo com os resultados laboratoriais e clínicos dos pacientes. Foi realizada eletroforese em gel de poliacrilamida com dodecil sulfato de sódio (SDS-PAGE) para separação das proteínas de T. gondii. A técnica de Immunoblotting foi aplicada para a análise do perfil de proteínas imunorreativas à anticorpos IgG anti-T.gondii, utilizando membranas de nitrocelulose sensibilizadas com proteínas do parasito. Resultados: Foram analisadas 116 amostras de soro. Na comparação do perfil de proteínas imunorreativas a anticorpos presentes nas amostras dos diferentes grupos e subgrupos, as proteínas de 150, 18.5 e 16.96 kDa foram prevalentemente reconhecidas pelas amostras do grupo de infecção adquirida. As proteínas de 343, 189, 150, 75 e 42 kDa apresentaram maiores chances de ser detectadas pelo subgrupo de infecção congênita sintomática, enquanto as proteínas de 61, 50 e 16.96 kDa foram reconhecidas significativamente pelo subgrupo de infecção adquirida aguda. Conclusões: Diante dos resultados obtidos foi possível identificar potenciais biomarcadores que poderão auxiliar no diagnóstico e tratamento precoce da toxoplasmose, evitando o aparecimento de manifestações clínicas no decorrer da vida de crianças congenitamente infectadas.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2021-01-13T14:16:54Z No. of bitstreams: 2 license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Tese - Heloisa Ribeiro Storchilo - 2020.pdf: 4873615 bytes, checksum: c2cc75104aaeea299001e239a2123dc2 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2021-01-15T12:00:19Z (GMT) No. of bitstreams: 2 license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Tese - Heloisa Ribeiro Storchilo - 2020.pdf: 4873615 bytes, checksum: c2cc75104aaeea299001e239a2123dc2 (MD5)Made available in DSpace on 2021-01-15T12:00:19Z (GMT). No. of bitstreams: 2 license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Tese - Heloisa Ribeiro Storchilo - 2020.pdf: 4873615 bytes, checksum: c2cc75104aaeea299001e239a2123dc2 (MD5) Previous issue date: 2020-11-27Fundação de Amparo à Pesquisa do Estado de GoiásOutroporUniversidade Federal de GoiásPrograma de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessImmunoblottingPrognósticoToxoplasmose aguda e toxoplasmose congênitaImmunoblottingPrognosisAcute toxoplasmosis and congenital toxoplasmosisCIENCIAS DA SAUDE::SAUDE COLETIVA::SAUDE PUBLICAIdentificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênitaIdentification of biomarkers for the diagnosis of acute and congenital toxoplasmosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis7050050050050050028113835reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/0c8db46f-a1b4-4830-895e-0bb5630e484a/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/e8c587ed-b7a4-423a-b2eb-6f3c385c01c1/download4460e5956bc1d1639be9ae6146a50347MD52ORIGINALTese - Heloisa Ribeiro Storchilo - 2020.pdfTese - Heloisa Ribeiro Storchilo - 2020.pdfapplication/pdf4873615http://repositorio.bc.ufg.br/tede/bitstreams/6bc1cdee-f88c-4c58-b39b-8be40a140ab4/downloadc2cc75104aaeea299001e239a2123dc2MD53tede/110332021-01-15 09:00:20.272http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/11033http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2021-01-15T12:00:20Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.pt_BR.fl_str_mv |
Identificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênita |
dc.title.alternative.eng.fl_str_mv |
Identification of biomarkers for the diagnosis of acute and congenital toxoplasmosis |
title |
Identificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênita |
spellingShingle |
Identificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênita Storchilo, Heloisa Ribeiro Immunoblotting Prognóstico Toxoplasmose aguda e toxoplasmose congênita Immunoblotting Prognosis Acute toxoplasmosis and congenital toxoplasmosis CIENCIAS DA SAUDE::SAUDE COLETIVA::SAUDE PUBLICA |
title_short |
Identificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênita |
title_full |
Identificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênita |
title_fullStr |
Identificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênita |
title_full_unstemmed |
Identificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênita |
title_sort |
Identificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênita |
author |
Storchilo, Heloisa Ribeiro |
author_facet |
Storchilo, Heloisa Ribeiro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Castro, Ana Maria de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9232309971000621 |
dc.contributor.referee1.fl_str_mv |
Borges, Clayton Luiz |
dc.contributor.referee2.fl_str_mv |
Alves, Daniella de Sousa Mendes Moreira |
dc.contributor.referee3.fl_str_mv |
Rezende, Hanstter Hallison Alves |
dc.contributor.referee4.fl_str_mv |
Paccez, Juliano Domiraci |
dc.contributor.referee5.fl_str_mv |
Castro, Ana Maria de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3543813852521316 |
dc.contributor.author.fl_str_mv |
Storchilo, Heloisa Ribeiro |
contributor_str_mv |
Castro, Ana Maria de Borges, Clayton Luiz Alves, Daniella de Sousa Mendes Moreira Rezende, Hanstter Hallison Alves Paccez, Juliano Domiraci Castro, Ana Maria de |
dc.subject.por.fl_str_mv |
Immunoblotting Prognóstico Toxoplasmose aguda e toxoplasmose congênita |
topic |
Immunoblotting Prognóstico Toxoplasmose aguda e toxoplasmose congênita Immunoblotting Prognosis Acute toxoplasmosis and congenital toxoplasmosis CIENCIAS DA SAUDE::SAUDE COLETIVA::SAUDE PUBLICA |
dc.subject.eng.fl_str_mv |
Immunoblotting Prognosis Acute toxoplasmosis and congenital toxoplasmosis |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::SAUDE COLETIVA::SAUDE PUBLICA |
description |
Introduction: The diagnosis of congenital toxoplasmosis is complex and might take months to confirm since most newborns do not present symptoms and levels of undetected IgM and IgA anti-Toxoplasma gondii antibodies. Besides, pregnant women may have residual IgM, making it difficult to diagnose acute infection during pregnancy. Despite the various serological tests developed, there are few studies about biomarkers that can assist in the diagnosis of acute and congenital toxoplasmosis. Objective: Analyze the immunoreactivity profile of T. gondii protein with the potential to be biomarkers for the diagnosis of acute toxoplasmosis and the diagnosis and prognosis of congenital toxoplasmosis. Methods: Serum samples were selected from children with symptomatic and asymptomatic congenital toxoplasmosis and women of childbearing age or pregnant women with acute and chronic acquired toxoplasmosis. These samples were grouped according to the patients' laboratory and clinical results. Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) was used to separate T. gondii proteins. The Immunoblotting technique was applied to the analysis of immunoreactive bands profile by IgG antibodies, using nitrocellulose membranes sensitized with parasite proteins. Results: One hundred and sixteen samples were analyzed. When comparing immunoreactive bands profile for antibodies present in samples from different groups and subgroups, the 150, 18.5, and 16.96 kDa proteins were more immunoreactive by the antibodies present in serum samples from the acquired infection group. The 343, 189, 150, 75, and 42 kDa proteins showed more chance to be detected by the symptomatic congenital infection subgroup, while the 61, 50, and 16.96 kDa proteins were significantly immunoreactive by the acute infection subgroup. Conclusions: Before obtained results, it was possible to identify potential biomarkers that may assist in early diagnosis and treatment of toxoplasmosis, avoiding the appearance of clinical manifestations throughout the life of children congenitally infected. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-11-27 |
dc.date.accessioned.fl_str_mv |
2021-01-15T12:00:19Z |
dc.date.available.fl_str_mv |
2021-01-15T12:00:19Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
STORCHILO, H. R. Identificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênita. 2020. 110 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2020. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/11033 |
dc.identifier.dark.fl_str_mv |
ark:/38995/001300000dsdk |
identifier_str_mv |
STORCHILO, H. R. Identificação de biomarcadores para o diagnóstico da toxoplasmose aguda e congênita. 2020. 110 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2020. ark:/38995/001300000dsdk |
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http://repositorio.bc.ufg.br/tede/handle/tede/11033 |
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por |
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por |
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1138 |
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3 5 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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Universidade Federal de Goiás |
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Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP) |
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UFG |
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Brasil |
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Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
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Universidade Federal de Goiás |
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