Padronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellus
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/7586 |
Resumo: | The genus Brachiaria has provided important forage species to the countries located in tropical regions. However, this genus of grass is associated with photosensitization and poisoning in animal production. Thus, the aim of this study was to establish the standard for collection, processing, and conservation of Brachiaria brizantha for protodioscin quantification and spore count from Pithomyces chartarum and the evaluation of guinea pig (Cavia porcellus) as an experimental model of poisoning by diosgenin. For Brachiaria brizantha evaluation, samples were collected from eight pastures, which were subdivided into young leaves, mature leaves, old leaves, and whole plant. After that, the samples were submitted to nine different drying and conservation treatments. After separation and treatment of the samples, saponins were quantified and spores were counted. In most of treatments, the young leaves presented larger amounts of protodioscin than the old leaves and the whole plant. The treatments that maintained the highest amounts of protodioscin were room temperature, oven, and oven with forced air circulation. No fungal spores were found in the pasture samples. Thus, we concluded that the concentration of protodioscin varies with the maturation stage of the leaves; drying at room temperature, in an oven, or in an oven with forced air circulation are the best methods, and freezing plants for preservation of protodioscin is not recommended. In the guinea pigs evaluation, 14 guinea pigs were divided into two groups, the treatment group (TG) and the control group (CG). The TG received 480 mg/kg of diosgenin and the CG received only vegetable oil. Both groups received the treatment for 30 days. Blood samples were collected on day 0 (M0) and day 30 (M30) for blood cell count and biochemical analyzes. In clinical biochemistry, the analyzes were performed for alanine aminotransferase, aspartate aminotransferase, gamma glutamyl trasferase, alkaline phosphatase, urea, creatinine, glucose, cholesterol, HDL, triglycerides, lactate, cholinesterase, and calcium. Hematology and biochemistry did not present alterations, which could not be related to hepatic disease caused by saponins. In histology, only one animal had bile duct hyperplasia, the other alterations observed were generally nonspecific. Therefore, we concluded that the administration of 480 mg/kg of diosgenin to guinea pigs did not produce significant hepatic lesions or hematological and biochemical alterations related to liver disease. |
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Fioravanti, Maria Clorinda Soareshttp://lattes.cnpq.br/8772502020076257Arnhold, Emmanuelhttp://lattes.cnpq.br/7156945506134934Riet-Correa, Franklinhttp://lattes.cnpq.br/4742586492304200Fioravanti, Maria Clorinda SoaresBorges, José Renato JunqueiraSilva, Thiago Carvalho daCunha, Paulo Henrique Jorge daAraújo, Eugênio Gonçalves dehttp://lattes.cnpq.br/5221749202342407Goulart, Daniel Silva2017-07-18T10:31:42Z2017-03-02GOULART, D. S. Padronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellus. 2017. 94 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2017.http://repositorio.bc.ufg.br/tede/handle/tede/7586The genus Brachiaria has provided important forage species to the countries located in tropical regions. However, this genus of grass is associated with photosensitization and poisoning in animal production. Thus, the aim of this study was to establish the standard for collection, processing, and conservation of Brachiaria brizantha for protodioscin quantification and spore count from Pithomyces chartarum and the evaluation of guinea pig (Cavia porcellus) as an experimental model of poisoning by diosgenin. For Brachiaria brizantha evaluation, samples were collected from eight pastures, which were subdivided into young leaves, mature leaves, old leaves, and whole plant. After that, the samples were submitted to nine different drying and conservation treatments. After separation and treatment of the samples, saponins were quantified and spores were counted. In most of treatments, the young leaves presented larger amounts of protodioscin than the old leaves and the whole plant. The treatments that maintained the highest amounts of protodioscin were room temperature, oven, and oven with forced air circulation. No fungal spores were found in the pasture samples. Thus, we concluded that the concentration of protodioscin varies with the maturation stage of the leaves; drying at room temperature, in an oven, or in an oven with forced air circulation are the best methods, and freezing plants for preservation of protodioscin is not recommended. In the guinea pigs evaluation, 14 guinea pigs were divided into two groups, the treatment group (TG) and the control group (CG). The TG received 480 mg/kg of diosgenin and the CG received only vegetable oil. Both groups received the treatment for 30 days. Blood samples were collected on day 0 (M0) and day 30 (M30) for blood cell count and biochemical analyzes. In clinical biochemistry, the analyzes were performed for alanine aminotransferase, aspartate aminotransferase, gamma glutamyl trasferase, alkaline phosphatase, urea, creatinine, glucose, cholesterol, HDL, triglycerides, lactate, cholinesterase, and calcium. Hematology and biochemistry did not present alterations, which could not be related to hepatic disease caused by saponins. In histology, only one animal had bile duct hyperplasia, the other alterations observed were generally nonspecific. Therefore, we concluded that the administration of 480 mg/kg of diosgenin to guinea pigs did not produce significant hepatic lesions or hematological and biochemical alterations related to liver disease.O gênero Brachiaria possui importantes espécies de forrageiras utilizadas em países localizados em regiões tropicais. No entanto, esse gênero de gramíneas está associado à fotossensibilização e intoxicações em animais de produção. Deste modo, objetivou-se com este estudo estabelecer o padrão para colheita, processamento e conservação de amostras de Brachiaria brizantha para quantificação de protodioscina e contagem de esporos de Pithomyces chartarum e a avaliação da Cavia porcellus (cobaias) como modelo experimental na intoxicação por diosgenina. Para a avaliação da Brachiaria brizantha, amostras foram colhidas de oito pastagens as quais foram subdivididas em folhas jovens, folhas maduras, folhas velhas e planta inteira e submetidas a nove diferentes métodos de processamento. Após separação e tratamento das amostras foi realizada a quantificação de saponinas e a contagem de esporos. Observou-se que na maioria dos tratamentos as folhas jovens apresentaram quantidades maiores de protodioscina que as folhas velhas e a planta inteira. Os tratamentos que mantiveram as maiores quantidades de protodioscina foram temperatura ambiente, estufa e estufa com ventilação forçada. Nas amostras das pastagens não foram encontrados esporos do fungo. Desta forma, conclui-se que a concentração de protodioscina é maior nas folhas jovens; a secagem em temperatura ambiente, em estufa ou em estufa de ventilação forçada são os melhores métodos para preservação de protodioscina e o congelamento de plantas para a quantificação de protodioscina não é recomendado. Para a avaliação das cobaias como modelos experimentais na intoxicação por diosgenina foram utilizadas 14 cobaias, divididas em dois grupos, o grupo tratado (GT) e o grupo controle (GC). O GT recebeu 480 mg/kg de diosgenina diluída em óleo vegetal e o GC recebeu somente o óleo vegetal, ambos por 30 dias. Para avaliação do hemograma e análises bioquímicas foram colhidas amostras sanguíneas no dia 0 (M0) e no dia 30 (M30). Na bioquímica plasmática foram realizadas análises para ALT, AST, GGT, ALP, ureia, creatinina, glicose, colesterol, HDL, triglicerídeos, lactato, colinesterase e cálcio. A hematologia e a bioquímica não apresentaram alterações relacionadas à doença hepática causada por saponinas. Na histologia apenas um animal apresentou hiperplasia de ductos biliares, as outras alterações observadas foram de forma geral inespecíficas. Conclui-se que, na dose utilizada, a administração da diosgenina a cobaias não produz lesões hepáticas expressivas nem alterações hematológicas e bioquímicas relacionadas a hepatopatia.Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2017-07-17T11:35:24Z No. of bitstreams: 2 Tese - Daniel Silva Goulart -2017.pdf: 3553497 bytes, checksum: 6ec13ae3cfbad63a4fdfdcf4e9b0dab1 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-07-18T10:31:42Z (GMT) No. of bitstreams: 2 Tese - Daniel Silva Goulart -2017.pdf: 3553497 bytes, checksum: 6ec13ae3cfbad63a4fdfdcf4e9b0dab1 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2017-07-18T10:31:42Z (GMT). No. of bitstreams: 2 Tese - Daniel Silva Goulart -2017.pdf: 3553497 bytes, checksum: 6ec13ae3cfbad63a4fdfdcf4e9b0dab1 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-03-02Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciência Animal (EVZ)UFGBrasilEscola de Veterinária e Zootecnia - EVZ (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessBrachiaria brizanthaDiosgeninaFotossensibilizaçãoModelo experimentalProtodioscinaBrachiaria brizanthaDiosgeninExperimental modelPhotosensitizationProtodioscinPATOLOGIA ANIMAL::PATOLOGIA CLINICA ANIMALCLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMALPadronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellusStandardization for harvesting and conservation of Brachiaria samples for quantification of saponin and subchronic toxicity of diosgenin in Cavia porcellusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis4581960685150189167600600600600600-62175521142490945826109832347911310615710819066431151437-2555911436985713659reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.eng.fl_str_mv |
Padronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellus |
dc.title.alternative.eng.fl_str_mv |
Standardization for harvesting and conservation of Brachiaria samples for quantification of saponin and subchronic toxicity of diosgenin in Cavia porcellus |
title |
Padronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellus |
spellingShingle |
Padronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellus Goulart, Daniel Silva Brachiaria brizantha Diosgenina Fotossensibilização Modelo experimental Protodioscina Brachiaria brizantha Diosgenin Experimental model Photosensitization Protodioscin PATOLOGIA ANIMAL::PATOLOGIA CLINICA ANIMAL CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL |
title_short |
Padronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellus |
title_full |
Padronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellus |
title_fullStr |
Padronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellus |
title_full_unstemmed |
Padronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellus |
title_sort |
Padronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellus |
author |
Goulart, Daniel Silva |
author_facet |
Goulart, Daniel Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fioravanti, Maria Clorinda Soares |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8772502020076257 |
dc.contributor.advisor-co1.fl_str_mv |
Arnhold, Emmanuel |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7156945506134934 |
dc.contributor.advisor-co2.fl_str_mv |
Riet-Correa, Franklin |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/4742586492304200 |
dc.contributor.referee1.fl_str_mv |
Fioravanti, Maria Clorinda Soares |
dc.contributor.referee2.fl_str_mv |
Borges, José Renato Junqueira |
dc.contributor.referee3.fl_str_mv |
Silva, Thiago Carvalho da |
dc.contributor.referee4.fl_str_mv |
Cunha, Paulo Henrique Jorge da |
dc.contributor.referee5.fl_str_mv |
Araújo, Eugênio Gonçalves de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5221749202342407 |
dc.contributor.author.fl_str_mv |
Goulart, Daniel Silva |
contributor_str_mv |
Fioravanti, Maria Clorinda Soares Arnhold, Emmanuel Riet-Correa, Franklin Fioravanti, Maria Clorinda Soares Borges, José Renato Junqueira Silva, Thiago Carvalho da Cunha, Paulo Henrique Jorge da Araújo, Eugênio Gonçalves de |
dc.subject.por.fl_str_mv |
Brachiaria brizantha Diosgenina Fotossensibilização Modelo experimental Protodioscina |
topic |
Brachiaria brizantha Diosgenina Fotossensibilização Modelo experimental Protodioscina Brachiaria brizantha Diosgenin Experimental model Photosensitization Protodioscin PATOLOGIA ANIMAL::PATOLOGIA CLINICA ANIMAL CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL |
dc.subject.eng.fl_str_mv |
Brachiaria brizantha Diosgenin Experimental model Photosensitization Protodioscin |
dc.subject.cnpq.fl_str_mv |
PATOLOGIA ANIMAL::PATOLOGIA CLINICA ANIMAL CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL |
description |
The genus Brachiaria has provided important forage species to the countries located in tropical regions. However, this genus of grass is associated with photosensitization and poisoning in animal production. Thus, the aim of this study was to establish the standard for collection, processing, and conservation of Brachiaria brizantha for protodioscin quantification and spore count from Pithomyces chartarum and the evaluation of guinea pig (Cavia porcellus) as an experimental model of poisoning by diosgenin. For Brachiaria brizantha evaluation, samples were collected from eight pastures, which were subdivided into young leaves, mature leaves, old leaves, and whole plant. After that, the samples were submitted to nine different drying and conservation treatments. After separation and treatment of the samples, saponins were quantified and spores were counted. In most of treatments, the young leaves presented larger amounts of protodioscin than the old leaves and the whole plant. The treatments that maintained the highest amounts of protodioscin were room temperature, oven, and oven with forced air circulation. No fungal spores were found in the pasture samples. Thus, we concluded that the concentration of protodioscin varies with the maturation stage of the leaves; drying at room temperature, in an oven, or in an oven with forced air circulation are the best methods, and freezing plants for preservation of protodioscin is not recommended. In the guinea pigs evaluation, 14 guinea pigs were divided into two groups, the treatment group (TG) and the control group (CG). The TG received 480 mg/kg of diosgenin and the CG received only vegetable oil. Both groups received the treatment for 30 days. Blood samples were collected on day 0 (M0) and day 30 (M30) for blood cell count and biochemical analyzes. In clinical biochemistry, the analyzes were performed for alanine aminotransferase, aspartate aminotransferase, gamma glutamyl trasferase, alkaline phosphatase, urea, creatinine, glucose, cholesterol, HDL, triglycerides, lactate, cholinesterase, and calcium. Hematology and biochemistry did not present alterations, which could not be related to hepatic disease caused by saponins. In histology, only one animal had bile duct hyperplasia, the other alterations observed were generally nonspecific. Therefore, we concluded that the administration of 480 mg/kg of diosgenin to guinea pigs did not produce significant hepatic lesions or hematological and biochemical alterations related to liver disease. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-07-18T10:31:42Z |
dc.date.issued.fl_str_mv |
2017-03-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GOULART, D. S. Padronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellus. 2017. 94 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2017. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/7586 |
identifier_str_mv |
GOULART, D. S. Padronização para colheita e conservação de amostras de braquiária para quantificação de saponina e toxidade subcrônica da diosgenina em Cavia porcellus. 2017. 94 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2017. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/7586 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
4581960685150189167 |
dc.relation.confidence.fl_str_mv |
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