Caracterização histológica de vermes adultos machos de Lagochilascaris minor
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/3680 |
Resumo: | Nematoda Lagochilascaris minor is the etiologic agent of lagochilascaríase in human beings. Until today, no one knows the natural host of this helminth and Brazil registers about 90% of the cases of lagochilascaríase world-wide human being. The life cycle of L. minor was described using an experimental model in mice and domestic cat, behaving as an intermediate host and definitive host respectively. The objective of this study was to evaluate the histology of the adult males of this helminth worms, using light microscopy. These specimens were removed after necropsy of cats kept in their cages cataloged in male and female worms, and the adult male specimens were fixed in formalin solution 10 % hot, AFA and 4% paraformaldehyde in sodium cacodylate buffer 0 1 M, pH 7.2. After the different fixations, the samples were dehydrated in a gradual series of ethanol in different concentrations of alcohol levels, and included in historesin cut with 3μm –thick microtome, with horizontal and transverse serial sections. The sections were stained with Hematoxylin & Eosin (HE), Carmin, Giemsa and Toluidine Blue. The best fixation was achieved with the use of AFA and 4% paraformaldehyde in 0.1 M sodium cacodylate buffer pH 7.2 and the best staining was obtained from the use of the dye HE, which showed histological structures of adult male worms L. minor loop as testicular, seminal vesicle, ejaculatory duct, sperm and spicule. It was also observed structures of the digestive system, muscular system, cuticle, wing side and lateral line. The sections were photomicrographed using photomicroscope with the help of the program Motic Images Plus 2.0, selected and analyzed. |
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Bezerra, José Clecildo Barretohttp://lattes.cnpq.br/9491755585617846Barbosa, Alverne PassosBezerra, José Clecildo BarretoOliveira, Jayrson Araújo deCampos, Dulcinéa Maria Barbosahttp://lattes.cnpq.br/4342249399592371Vieira, Ana Paula de Toledo2014-11-24T14:02:12Z2012-02-27VIEIRA, Ana Paula de Toledo. Caracterização histológica de vermes adultos machos de Lagochilascaris minor. 2012. 66 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2012.http://repositorio.bc.ufg.br/tede/handle/tede/3680ark:/38995/0013000008wp3Nematoda Lagochilascaris minor is the etiologic agent of lagochilascaríase in human beings. Until today, no one knows the natural host of this helminth and Brazil registers about 90% of the cases of lagochilascaríase world-wide human being. The life cycle of L. minor was described using an experimental model in mice and domestic cat, behaving as an intermediate host and definitive host respectively. The objective of this study was to evaluate the histology of the adult males of this helminth worms, using light microscopy. These specimens were removed after necropsy of cats kept in their cages cataloged in male and female worms, and the adult male specimens were fixed in formalin solution 10 % hot, AFA and 4% paraformaldehyde in sodium cacodylate buffer 0 1 M, pH 7.2. After the different fixations, the samples were dehydrated in a gradual series of ethanol in different concentrations of alcohol levels, and included in historesin cut with 3μm –thick microtome, with horizontal and transverse serial sections. The sections were stained with Hematoxylin & Eosin (HE), Carmin, Giemsa and Toluidine Blue. The best fixation was achieved with the use of AFA and 4% paraformaldehyde in 0.1 M sodium cacodylate buffer pH 7.2 and the best staining was obtained from the use of the dye HE, which showed histological structures of adult male worms L. minor loop as testicular, seminal vesicle, ejaculatory duct, sperm and spicule. It was also observed structures of the digestive system, muscular system, cuticle, wing side and lateral line. The sections were photomicrographed using photomicroscope with the help of the program Motic Images Plus 2.0, selected and analyzed.O nematoda Lagochilascaris minor é o agente etiológico da lagochilascaríase em humanos. Até hoje, não se conhece o hospedeiro natural deste helminto e o Brasil registra cerca de 90% dos casos da lagochilascaríase humana mundial. O ciclo evolutivo de L. minor foi descrito utilizando um modelo experimental com camundongo e gato doméstico, comportando-se como hospedeiro intermediário e hospedeiro definitivo, respectivamente. O objetivo deste trabalho foi avaliar a histologia dos vermes adultos machos deste helminto, utilizando microscopia de luz. Estes exemplares foram retirados após necropsia de gatos mantidos em biotério, catalogados em vermes machos e fêmeas, sendo que os exemplares machos adultos foram fixados em solução de Formalina a 10% a quente, AFA e Paraformaldeído a 4% em tampão cacodilato de sódio 0,1 M pH 7,2. Após as diferentes fixações, as amostras foram desidratadas em uma série gradual de etanol em diferentes concentrações de teores alcoólicos, incluídas em historesina e cortadas com 3μm de espessura em micrótomo, com secções seriadas horizontais e transversais. Os cortes foram corados com Hematoxilina & Eosina (HE), Carmim, Giemsa e Azul de Toluidina. A melhores fixações foram alcançadas com o uso de AFA e Paraformaldeído a 4% em tampão cacodilato sódio 0,1 M pH 7,2 e a melhor coloração obteve-se a partir da utilização do corante HE, que evidenciou estruturas histológicas dos vermes adultos machos de L. minor como alça testicular, vesícula seminal, ducto ejaculatório, espermatozoides e espículo. Observou-se também estruturas do sistema digestório, sistema muscular, cutícula, asa lateral e linha lateral. As imagens dos cortes foram realizadas utilizando um fotomicroscópio com o auxílio do programa Motic Images Plus 2.0, selecionadas e analisadas.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2014-11-24T13:11:26Z No. of bitstreams: 2 Dissertação - Ana Paula de Toledo Vieira - 2012.pdf: 10699833 bytes, checksum: 2e25915716db787ecacbd31491c5309a (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2014-11-24T14:02:12Z (GMT) No. of bitstreams: 2 Dissertação - Ana Paula de Toledo Vieira - 2012.pdf: 10699833 bytes, checksum: 2e25915716db787ecacbd31491c5309a (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2014-11-24T14:02:12Z (GMT). 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dc.title.por.fl_str_mv |
Caracterização histológica de vermes adultos machos de Lagochilascaris minor |
dc.title.alternative.eng.fl_str_mv |
Histological characterization of adult males worms of Lagochilascaris minor |
title |
Caracterização histológica de vermes adultos machos de Lagochilascaris minor |
spellingShingle |
Caracterização histológica de vermes adultos machos de Lagochilascaris minor Vieira, Ana Paula de Toledo Lagochilascaris minor Vermes adultos machos Microscopia Lagochilascaris minor Male adult worms Microscopy CLINICA MEDICA::DOENCAS INFECCIOSAS E PARASITARIAS |
title_short |
Caracterização histológica de vermes adultos machos de Lagochilascaris minor |
title_full |
Caracterização histológica de vermes adultos machos de Lagochilascaris minor |
title_fullStr |
Caracterização histológica de vermes adultos machos de Lagochilascaris minor |
title_full_unstemmed |
Caracterização histológica de vermes adultos machos de Lagochilascaris minor |
title_sort |
Caracterização histológica de vermes adultos machos de Lagochilascaris minor |
author |
Vieira, Ana Paula de Toledo |
author_facet |
Vieira, Ana Paula de Toledo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Bezerra, José Clecildo Barreto |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9491755585617846 |
dc.contributor.advisor-co1.fl_str_mv |
Barbosa, Alverne Passos |
dc.contributor.referee1.fl_str_mv |
Bezerra, José Clecildo Barreto |
dc.contributor.referee2.fl_str_mv |
Oliveira, Jayrson Araújo de |
dc.contributor.referee3.fl_str_mv |
Campos, Dulcinéa Maria Barbosa |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4342249399592371 |
dc.contributor.author.fl_str_mv |
Vieira, Ana Paula de Toledo |
contributor_str_mv |
Bezerra, José Clecildo Barreto Barbosa, Alverne Passos Bezerra, José Clecildo Barreto Oliveira, Jayrson Araújo de Campos, Dulcinéa Maria Barbosa |
dc.subject.por.fl_str_mv |
Lagochilascaris minor Vermes adultos machos Microscopia |
topic |
Lagochilascaris minor Vermes adultos machos Microscopia Lagochilascaris minor Male adult worms Microscopy CLINICA MEDICA::DOENCAS INFECCIOSAS E PARASITARIAS |
dc.subject.eng.fl_str_mv |
Lagochilascaris minor Male adult worms Microscopy |
dc.subject.cnpq.fl_str_mv |
CLINICA MEDICA::DOENCAS INFECCIOSAS E PARASITARIAS |
description |
Nematoda Lagochilascaris minor is the etiologic agent of lagochilascaríase in human beings. Until today, no one knows the natural host of this helminth and Brazil registers about 90% of the cases of lagochilascaríase world-wide human being. The life cycle of L. minor was described using an experimental model in mice and domestic cat, behaving as an intermediate host and definitive host respectively. The objective of this study was to evaluate the histology of the adult males of this helminth worms, using light microscopy. These specimens were removed after necropsy of cats kept in their cages cataloged in male and female worms, and the adult male specimens were fixed in formalin solution 10 % hot, AFA and 4% paraformaldehyde in sodium cacodylate buffer 0 1 M, pH 7.2. After the different fixations, the samples were dehydrated in a gradual series of ethanol in different concentrations of alcohol levels, and included in historesin cut with 3μm –thick microtome, with horizontal and transverse serial sections. The sections were stained with Hematoxylin & Eosin (HE), Carmin, Giemsa and Toluidine Blue. The best fixation was achieved with the use of AFA and 4% paraformaldehyde in 0.1 M sodium cacodylate buffer pH 7.2 and the best staining was obtained from the use of the dye HE, which showed histological structures of adult male worms L. minor loop as testicular, seminal vesicle, ejaculatory duct, sperm and spicule. It was also observed structures of the digestive system, muscular system, cuticle, wing side and lateral line. The sections were photomicrographed using photomicroscope with the help of the program Motic Images Plus 2.0, selected and analyzed. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-27 |
dc.date.accessioned.fl_str_mv |
2014-11-24T14:02:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VIEIRA, Ana Paula de Toledo. Caracterização histológica de vermes adultos machos de Lagochilascaris minor. 2012. 66 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/3680 |
dc.identifier.dark.fl_str_mv |
ark:/38995/0013000008wp3 |
identifier_str_mv |
VIEIRA, Ana Paula de Toledo. Caracterização histológica de vermes adultos machos de Lagochilascaris minor. 2012. 66 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2012. ark:/38995/0013000008wp3 |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/3680 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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8197219506590464977 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-7769011444564556288 |
dc.relation.cnpq.fl_str_mv |
1767748423488408711 |
dc.relation.sponsorship.fl_str_mv |
-961409807440757778 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Medicina Tropical e Saúde Pública (IPTSP) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
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UFG |
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Repositório Institucional da UFG |
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bitstream.checksum.fl_str_mv |
4afdbb8c545fd630ea7db775da747b2f 1e0094e9d8adcf16b18effef4ce7ed83 9da0b6dfac957114c6a7714714b86306 bd3efa91386c1718a7f26a329fdcb468 2e25915716db787ecacbd31491c5309a 0132b42162ad2018f2df8ea45708ecb7 a982631800347ea9313468c0ea9823ae |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1811721458526715904 |