Estudo da encapsulação do voriconazol em lipossomas: obtenção, caracterização e estudo de estabilidade

• Autor(a) principal: Benedetti, Naiara Ieza Gallo de Magalhães
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/00130000018mf
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/9055
• topic: Lipossomas; Vorizonazol; Teste de estabilidade; Liposomes; Voriconazole; Stability test; CIENCIAS DA SAUDE::FARMACIA

Nanotechnology has grown in recent years, mainly in the pharmaceutical area, responsible for improving problems inherent to drugs and formulation limitations. Among the drug nanocarriers, liposomes are the most studied. They were discovered more than 50 years ago and are mostly produced by biodegradable and biocompatible materials, capable of encapsulating both hydrophilic and lipophilic drugs. Currently there are some liposomal formulations approved by regulatory agencies and available for several treatments. Voriconazole is a second generation triazole antifungal drug, with a broad spectrum of action. It is used primarily in cases of resistant fungal infections, although its conventional formulation comes with some problems, such as the use of SBECD. Therefore, the present work aimed to develop, characterize and evaluate the stability of liposomes containing voriconazole. Three production techniques and the highest drug concentration, capable of being encapsulated by the liposomes, were evaluated. The particles were characterized according to the liposomes guidelines, published by Food and Drug Administration, lyophilized and tested for stability at 4º, 25º and 40º C for 120 days. Particles of 100 nm diameter containing 2 mg/ml of voriconazole were developed and lyophilized using only 7% sucrose. A method was developed and validated by HPLC-ELSD to quantify the vesicles structural components and to monitor the degradation products during stability test.