Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Amanda Rafaela
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
dARK ID: ark:/38995/001300000cc3d
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/4912
Resumo: The genus Trichoderma is represented by non-pathogenic soil fungi that have been studied by act as biological control agents against fungal pathogens, such as Sclerotinia sclerotiorum, the fungus that causes white mold, besides that, reduce environment and human health risks by being able to replace agrochemicals. Proteomic strategies with MS techniques have been important tools in studies of patterns identification for protein expression in different growth conditions. This work aims to development new strategies that enable the detection and identification of proteins secreted by Trichoderma asperellum (TR356) and Sclerotinia sclerotiorum when inoculated together and separated. The results obtained by MALDI / TOF analysis allowed the identification of a β-1.3--glucanosiltransferase and α-1.2-Dmannosidase, demonstrating the possibility of proteins identification for better comprehension about interaction between these organisms. It was therefore possible to identify the proteins through strategies used, but further analysis are required in order to elucidate the function and interaction of proteins secreted by the fungus Trichoderma asperellum TR356 against S. sclerotiorum.
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spelling Ulhoa, Cirano Joséhttp://lattes.cnpq.br/8368469162867277Ulhoa, Cirano JoséMonteiro, Valdirene NevesFernandes, Kátia Flaviahttp://lattes.cnpq.br/8588374897705119Rodrigues, Amanda Rafaela2015-11-19T10:17:31Z2014-10-23RODRIGUES, A. R. Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum. 2014.86 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2014.http://repositorio.bc.ufg.br/tede/handle/tede/4912ark:/38995/001300000cc3dThe genus Trichoderma is represented by non-pathogenic soil fungi that have been studied by act as biological control agents against fungal pathogens, such as Sclerotinia sclerotiorum, the fungus that causes white mold, besides that, reduce environment and human health risks by being able to replace agrochemicals. Proteomic strategies with MS techniques have been important tools in studies of patterns identification for protein expression in different growth conditions. This work aims to development new strategies that enable the detection and identification of proteins secreted by Trichoderma asperellum (TR356) and Sclerotinia sclerotiorum when inoculated together and separated. The results obtained by MALDI / TOF analysis allowed the identification of a β-1.3--glucanosiltransferase and α-1.2-Dmannosidase, demonstrating the possibility of proteins identification for better comprehension about interaction between these organisms. It was therefore possible to identify the proteins through strategies used, but further analysis are required in order to elucidate the function and interaction of proteins secreted by the fungus Trichoderma asperellum TR356 against S. sclerotiorum.OgêneroTrichodermaérepresentadoporfungosdesolonãopatogênicosquesãoestudad osporsuaaçãocomoagentesdecontrolebiológicocontrafungosfitopatógenos,comoScler otiniasclerotiorum,ofungocausadordomofobranco.Osfungos Trichoderma spp.atuamcomoimportantesagentesdecontrolebiológico,diminuindoosriscosàsaúdehu manaeaomeioambienteporseremcapazesdesubstituirosagroquímicos.Estratégiasprot eômicas juntamente com técnicas de MSsãoimportantesferramentasemestudosdepadrão, identificação e expressãodeproteínasemdiferentescondiçõesdecrescimento.Levando em consideração a importância de tais estudos, o presente trabalho tem por objetivo o desenvolvimento de novas estratégias que possibilitem a detecção e identificação de proteínas secretadas por Trichoderma asperellum (TR356) e Sclerotinia sclerotiorum quando inoculados juntos e/ou separados os dois organismos vivos. Os resultados obtidos através das análises por MALDI/TOF permitiram a identificação dasproteínas, β-1,3-glucanosiltransferase e α-1,2-D-mannosidase,demonstrando a possibilidade de identificaçãopara o entendimento futuro acerca da interação destes organismos. Foi possível, portanto, a identificação das proteínas através das estratégias utilizadas, porém serão necessárias futuras análises afim de elucidar a interação e função das proteínas secretadas através do fungo T. asperellum TR356sobre S. sclerotiorum.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2015-11-19T10:15:55Z No. of bitstreams: 2 Dissertação - Amanda Rafaela Rodrigues - 2014.pdf: 1648839 bytes, checksum: 9a00f301e577d05e94214afe813688c5 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-11-19T10:17:31Z (GMT) No. of bitstreams: 2 Dissertação - Amanda Rafaela Rodrigues - 2014.pdf: 1648839 bytes, checksum: 9a00f301e577d05e94214afe813688c5 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2015-11-19T10:17:31Z (GMT). No. of bitstreams: 2 Dissertação - Amanda Rafaela Rodrigues - 2014.pdf: 1648839 bytes, checksum: 9a00f301e577d05e94214afe813688c5 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-10-23Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessTrichoderma asperellumS. sclerotiorumMALDI/TOFSecretomaTrichoderma asperellumS. sclerotiorumMALDI/TOFSecretomeCIENCIAS BIOLOGICAS::BIOLOGIA GERALAnálise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorumAnalysis of Trichoderma asperellum (TR356) secretome in response to plant pathogenic fungus Sclerotinia sclerotioruminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6883982777473437920600600600600-3872772117827373404-16345593859312446972075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum
dc.title.alternative.por.fl_str_mv Analysis of Trichoderma asperellum (TR356) secretome in response to plant pathogenic fungus Sclerotinia sclerotiorum
title Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum
spellingShingle Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum
Rodrigues, Amanda Rafaela
Trichoderma asperellum
S. sclerotiorum
MALDI/TOF
Secretoma
Trichoderma asperellum
S. sclerotiorum
MALDI/TOF
Secretome
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum
title_full Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum
title_fullStr Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum
title_full_unstemmed Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum
title_sort Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum
author Rodrigues, Amanda Rafaela
author_facet Rodrigues, Amanda Rafaela
author_role author
dc.contributor.advisor1.fl_str_mv Ulhoa, Cirano José
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8368469162867277
dc.contributor.referee1.fl_str_mv Ulhoa, Cirano José
dc.contributor.referee2.fl_str_mv Monteiro, Valdirene Neves
dc.contributor.referee3.fl_str_mv Fernandes, Kátia Flavia
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8588374897705119
dc.contributor.author.fl_str_mv Rodrigues, Amanda Rafaela
contributor_str_mv Ulhoa, Cirano José
Ulhoa, Cirano José
Monteiro, Valdirene Neves
Fernandes, Kátia Flavia
dc.subject.por.fl_str_mv Trichoderma asperellum
S. sclerotiorum
MALDI/TOF
Secretoma
topic Trichoderma asperellum
S. sclerotiorum
MALDI/TOF
Secretoma
Trichoderma asperellum
S. sclerotiorum
MALDI/TOF
Secretome
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.eng.fl_str_mv Trichoderma asperellum
S. sclerotiorum
MALDI/TOF
Secretome
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description The genus Trichoderma is represented by non-pathogenic soil fungi that have been studied by act as biological control agents against fungal pathogens, such as Sclerotinia sclerotiorum, the fungus that causes white mold, besides that, reduce environment and human health risks by being able to replace agrochemicals. Proteomic strategies with MS techniques have been important tools in studies of patterns identification for protein expression in different growth conditions. This work aims to development new strategies that enable the detection and identification of proteins secreted by Trichoderma asperellum (TR356) and Sclerotinia sclerotiorum when inoculated together and separated. The results obtained by MALDI / TOF analysis allowed the identification of a β-1.3--glucanosiltransferase and α-1.2-Dmannosidase, demonstrating the possibility of proteins identification for better comprehension about interaction between these organisms. It was therefore possible to identify the proteins through strategies used, but further analysis are required in order to elucidate the function and interaction of proteins secreted by the fungus Trichoderma asperellum TR356 against S. sclerotiorum.
publishDate 2014
dc.date.issued.fl_str_mv 2014-10-23
dc.date.accessioned.fl_str_mv 2015-11-19T10:17:31Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv RODRIGUES, A. R. Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum. 2014.86 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2014.
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dc.identifier.dark.fl_str_mv ark:/38995/001300000cc3d
identifier_str_mv RODRIGUES, A. R. Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum. 2014.86 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2014.
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dc.publisher.department.fl_str_mv Instituto de Ciências Biológicas - ICB (RG)
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