Rastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/7001 |
Resumo: | Men who have sex with men (MSM) are more vulnerable to hepatitis B virus (HBV) infection when compared to the general population. In Brazil, the epidemiological profile of hepatitis B in MSM is still poorly understood. This study aimed to estimate the prevalence of HBV infection in MSM in Goiânia-Goiás and to analyze the associated factors, perform a molecular characterization of the viral isolates, as well as to investigate the vaccine situation against hepatitis B in this population. A cross-sectional study was conducted among MSM in the City of Goiânia, Central Brazil. From March to November 2014, a total of 522 participants were recruited using respondent-driven sampling (RDS). After signing the consent form, MSM were interviewed and a blood sample was collected. All samples were tested for HBV serological markers and HBV DNA. HBV isolates were sequenced for the determination of HBV genotypes and subgenotypes by phylogenetic analysis and for identification of mutations in viral genome. Data were analyzed using the RDSAT and SPSS programs. The overall prevalence of HBV infection (presence of anti-HBc marker) was 15.4% (95% CI: 8.7-25.8) and the rate of HBsAg carriers was 0.6% (95% CI: 0.2-1.6). Age over 25 years old, receptive anal intercourse, previous sex with women, and history of sexually transmitted infection (STI) were factors associated with HBV infection. Viral DNA was detected only in HBsAg-positive individuals, and occult HBV infection was not observed in the study population. HBV isolates were classified into genotype A (subgenotypes A1 and A2).Mutations in the S (T131N), basal core promoter (BCP) (A1762T/G1764A) and Pre-C/C (G1862T/G1888A and G1862T) regions of the HBV genome were identified. About 40% (95% CI: 32.3-48.8) of the participants had serological evidence of previous HBV vaccination (reactive for isolated anti-HBs), while 76.2% reported having previously received the vaccine. In addition, 44.3% (95% CI: 36.1-52.9) of MSM were susceptible to HBV infection. A greater frequency of positivity for anti-HBs alone was observed among MSM up to 25 years old, followed by declining vaccination with increasing age. By contrast, an increase in HBV infection with increasing age was found. HBV vaccination was associated with unprotected anal intercourse and history of STI (with a borderline p-value). In this study, we observed a poor agreement when comparing the self-reported and serologic evidence of HBV vaccination (k = 0.17). These results reveal a high overall prevalence of HBV infection in the study population associated with sexual risk behaviors, and in contrast, a low frequency of hepatitis B vaccination, evidencing a need for public health strategies to increase vaccine coverage in MSM, in addition to monitoring HBV carriers. |
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Martins, Regina Maria Bringelhttp://lattes.cnpq.br/2582896795892370Matos, Márcia Alves Dias dehttp://lattes.cnpq.br/0582530071710533Martins, Regina Maria BringelCastro, Ana Rita Coimbra Motta deLago, Bárbara VieiraTeles, Sheila AraújoAires, Rodrigo Sebbahttp://lattes.cnpq.br/1772687803415650Oliveira, Marina Pedroso de2017-03-24T11:03:37Z2017-02-21Oliveira, M. P. Rastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS). 2017.136 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2017.http://repositorio.bc.ufg.br/tede/handle/tede/7001Men who have sex with men (MSM) are more vulnerable to hepatitis B virus (HBV) infection when compared to the general population. In Brazil, the epidemiological profile of hepatitis B in MSM is still poorly understood. This study aimed to estimate the prevalence of HBV infection in MSM in Goiânia-Goiás and to analyze the associated factors, perform a molecular characterization of the viral isolates, as well as to investigate the vaccine situation against hepatitis B in this population. A cross-sectional study was conducted among MSM in the City of Goiânia, Central Brazil. From March to November 2014, a total of 522 participants were recruited using respondent-driven sampling (RDS). After signing the consent form, MSM were interviewed and a blood sample was collected. All samples were tested for HBV serological markers and HBV DNA. HBV isolates were sequenced for the determination of HBV genotypes and subgenotypes by phylogenetic analysis and for identification of mutations in viral genome. Data were analyzed using the RDSAT and SPSS programs. The overall prevalence of HBV infection (presence of anti-HBc marker) was 15.4% (95% CI: 8.7-25.8) and the rate of HBsAg carriers was 0.6% (95% CI: 0.2-1.6). Age over 25 years old, receptive anal intercourse, previous sex with women, and history of sexually transmitted infection (STI) were factors associated with HBV infection. Viral DNA was detected only in HBsAg-positive individuals, and occult HBV infection was not observed in the study population. HBV isolates were classified into genotype A (subgenotypes A1 and A2).Mutations in the S (T131N), basal core promoter (BCP) (A1762T/G1764A) and Pre-C/C (G1862T/G1888A and G1862T) regions of the HBV genome were identified. About 40% (95% CI: 32.3-48.8) of the participants had serological evidence of previous HBV vaccination (reactive for isolated anti-HBs), while 76.2% reported having previously received the vaccine. In addition, 44.3% (95% CI: 36.1-52.9) of MSM were susceptible to HBV infection. A greater frequency of positivity for anti-HBs alone was observed among MSM up to 25 years old, followed by declining vaccination with increasing age. By contrast, an increase in HBV infection with increasing age was found. HBV vaccination was associated with unprotected anal intercourse and history of STI (with a borderline p-value). In this study, we observed a poor agreement when comparing the self-reported and serologic evidence of HBV vaccination (k = 0.17). These results reveal a high overall prevalence of HBV infection in the study population associated with sexual risk behaviors, and in contrast, a low frequency of hepatitis B vaccination, evidencing a need for public health strategies to increase vaccine coverage in MSM, in addition to monitoring HBV carriers.Homens que fazem sexo com homens (HSH) apresentam maior vulnerabilidade para infecção pelo vírus da hepatite B (HBV) quando comparados à população em geral. No Brasil, o perfil epidemiológico da hepatite B em HSH ainda é pouco conhecido. Este estudo teve como objetivos estimar a prevalência da infecção pelo HBV em HSH em Goiânia-Goiás e analisar os fatores associados, realizar a caracterização molecular dos isolados virais, bem como investigar a situação vacinal contra hepatite B e fatores associados. Estudo transversal conduzido em HSH na região metropolitana de Goiânia-GO. De março a novembro de 2014, um total de 522 participantes foi recrutado utilizando o método respondent-driven sampling (RDS). Após assinatura do termo de consentimento livre e esclarecido, os HSH foram entrevistados e amostras sanguíneas coletadas. Todas as amostras foram testadas por ensaio imunoenzimático (ELISA) para detecção dos marcadores sorológicos da hepatite B e submetidas à extração e amplificação do HBV DNA. Os isolados do HBV foram sequenciados para determinação dos genótipos e subgenótipos do vírus por análise filogenética e para identificação de mutações no genoma viral. Os dados foram analisados utilizando os programas RDSAT e SPSS. A prevalência global da infecção pelo HBV (anti-HBc) foi de 15,4% (IC 95%: 8,7-25,8) e do HBsAg de 0,6% (IC 95%: 0,2-1,6). Idade acima de 25 anos, sexo anal receptivo, relação sexual com mulheres e história de infecções sexualmente transmissíveis (IST) foram fatores associados à infecção pelo HBV. O DNA viral foi detectado apenas nos indivíduos HBsAg positivos, não sendo observada infecção oculta na população estudada. Os isolados do HBV foram classificados como pertencentes ao genótipo A (subgenótipos A1 e A2) do HBV. Mutações nas regiões S (T131N), promotora basal do core (BCP) (A1762T/G1764A) e Pré-C/C (G1862T/G1888A e G1862T) do genoma viral foram identificadas. Cerca de 40% (IC 95%: 32,3-48,8) dos participantes tinham evidência sorológica de vacinação contra hepatite B (anti-HBs isolado), enquanto que 76,2% relataram ter recebido a vacina previamente. Além disso, 44,3% (IC 95%: 36,1-52,9) dos HSH eram suscetíveis à infecção pelo HBV. Observou-se uma maior positividade para anti-HBs isolado entre os HSH com até 25 anos de idade, seguido de seu declínio com o aumento da idade e, por outro lado, verificou-se aumento da infecção pelo HBV. A vacinação contra a hepatite B foi associada ao sexo anal desprotegido e marginalmente à história de IST. Neste estudo, notou-se, ainda, uma baixa concordância entre evidência sorológica e auto relato de vacinação (k = 0,17). Estes resultados revelam uma prevalência global elevada da infecção pelo HBV na população estudada e sua associação com comportamentos sexuais de risco e, em contraste, uma frequência baixa de vacinação contra hepatite B, evidenciando a necessidade de políticas públicas contínuas de saúde voltadas para os HSH, incluindo estratégias para aumentar a cobertura vacinal, além do monitoramento dos indivíduos portadores do HBV.Submitted by Erika Demachki (erikademachki@gmail.com) on 2017-03-23T21:02:36Z No. of bitstreams: 2 Tese - Marina Pedroso de Oliveira - 2017.pdf: 6913469 bytes, checksum: 0f52c792d453bdaabe154c7fca4f726b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-03-24T11:03:37Z (GMT) No. of bitstreams: 2 Tese - Marina Pedroso de Oliveira - 2017.pdf: 6913469 bytes, checksum: 0f52c792d453bdaabe154c7fca4f726b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2017-03-24T11:03:37Z (GMT). No. of bitstreams: 2 Tese - Marina Pedroso de Oliveira - 2017.pdf: 6913469 bytes, checksum: 0f52c792d453bdaabe154c7fca4f726b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-02-21Outroapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessHepatite BHSHEpidemiologiaVacinaçãoGenótiposMutaçõesHepatitis BMSMEpidemiologyVaccinationGenotypesMutationsCIENCIAS BIOLOGICAS::MICROBIOLOGIARastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS)Serological and molecular screening of hepatitis B virus among men who have sex with men in Goiânia-Goiás, using the respondent-driven sampling (RDS) methodinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis6085308344741430434600600600600-7769011444564556288-38545834699762208122442915598251853972reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALTese - Marina Pedroso de Oliveira - 2017.pdfTese - Marina Pedroso de Oliveira - 2017.pdfapplication/pdf4578460http://repositorio.bc.ufg.br/tede/bitstreams/75aa0f57-2fc1-4d95-beac-11c737d5ee99/download4c316ff1b229050b11ef25b4650e9b37MD55CC-LICENSElicense_urllicense_urltext/plain; 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dc.title.por.fl_str_mv |
Rastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS) |
dc.title.alternative.eng.fl_str_mv |
Serological and molecular screening of hepatitis B virus among men who have sex with men in Goiânia-Goiás, using the respondent-driven sampling (RDS) method |
title |
Rastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS) |
spellingShingle |
Rastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS) Oliveira, Marina Pedroso de Hepatite B HSH Epidemiologia Vacinação Genótipos Mutações Hepatitis B MSM Epidemiology Vaccination Genotypes Mutations CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
title_short |
Rastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS) |
title_full |
Rastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS) |
title_fullStr |
Rastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS) |
title_full_unstemmed |
Rastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS) |
title_sort |
Rastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS) |
author |
Oliveira, Marina Pedroso de |
author_facet |
Oliveira, Marina Pedroso de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Martins, Regina Maria Bringel |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2582896795892370 |
dc.contributor.advisor-co1.fl_str_mv |
Matos, Márcia Alves Dias de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0582530071710533 |
dc.contributor.referee1.fl_str_mv |
Martins, Regina Maria Bringel |
dc.contributor.referee2.fl_str_mv |
Castro, Ana Rita Coimbra Motta de |
dc.contributor.referee3.fl_str_mv |
Lago, Bárbara Vieira |
dc.contributor.referee4.fl_str_mv |
Teles, Sheila Araújo |
dc.contributor.referee5.fl_str_mv |
Aires, Rodrigo Sebba |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1772687803415650 |
dc.contributor.author.fl_str_mv |
Oliveira, Marina Pedroso de |
contributor_str_mv |
Martins, Regina Maria Bringel Matos, Márcia Alves Dias de Martins, Regina Maria Bringel Castro, Ana Rita Coimbra Motta de Lago, Bárbara Vieira Teles, Sheila Araújo Aires, Rodrigo Sebba |
dc.subject.por.fl_str_mv |
Hepatite B HSH Epidemiologia Vacinação Genótipos Mutações |
topic |
Hepatite B HSH Epidemiologia Vacinação Genótipos Mutações Hepatitis B MSM Epidemiology Vaccination Genotypes Mutations CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
dc.subject.eng.fl_str_mv |
Hepatitis B MSM Epidemiology Vaccination Genotypes Mutations |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
description |
Men who have sex with men (MSM) are more vulnerable to hepatitis B virus (HBV) infection when compared to the general population. In Brazil, the epidemiological profile of hepatitis B in MSM is still poorly understood. This study aimed to estimate the prevalence of HBV infection in MSM in Goiânia-Goiás and to analyze the associated factors, perform a molecular characterization of the viral isolates, as well as to investigate the vaccine situation against hepatitis B in this population. A cross-sectional study was conducted among MSM in the City of Goiânia, Central Brazil. From March to November 2014, a total of 522 participants were recruited using respondent-driven sampling (RDS). After signing the consent form, MSM were interviewed and a blood sample was collected. All samples were tested for HBV serological markers and HBV DNA. HBV isolates were sequenced for the determination of HBV genotypes and subgenotypes by phylogenetic analysis and for identification of mutations in viral genome. Data were analyzed using the RDSAT and SPSS programs. The overall prevalence of HBV infection (presence of anti-HBc marker) was 15.4% (95% CI: 8.7-25.8) and the rate of HBsAg carriers was 0.6% (95% CI: 0.2-1.6). Age over 25 years old, receptive anal intercourse, previous sex with women, and history of sexually transmitted infection (STI) were factors associated with HBV infection. Viral DNA was detected only in HBsAg-positive individuals, and occult HBV infection was not observed in the study population. HBV isolates were classified into genotype A (subgenotypes A1 and A2).Mutations in the S (T131N), basal core promoter (BCP) (A1762T/G1764A) and Pre-C/C (G1862T/G1888A and G1862T) regions of the HBV genome were identified. About 40% (95% CI: 32.3-48.8) of the participants had serological evidence of previous HBV vaccination (reactive for isolated anti-HBs), while 76.2% reported having previously received the vaccine. In addition, 44.3% (95% CI: 36.1-52.9) of MSM were susceptible to HBV infection. A greater frequency of positivity for anti-HBs alone was observed among MSM up to 25 years old, followed by declining vaccination with increasing age. By contrast, an increase in HBV infection with increasing age was found. HBV vaccination was associated with unprotected anal intercourse and history of STI (with a borderline p-value). In this study, we observed a poor agreement when comparing the self-reported and serologic evidence of HBV vaccination (k = 0.17). These results reveal a high overall prevalence of HBV infection in the study population associated with sexual risk behaviors, and in contrast, a low frequency of hepatitis B vaccination, evidencing a need for public health strategies to increase vaccine coverage in MSM, in addition to monitoring HBV carriers. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-03-24T11:03:37Z |
dc.date.issued.fl_str_mv |
2017-02-21 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Oliveira, M. P. Rastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS). 2017.136 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2017. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/7001 |
identifier_str_mv |
Oliveira, M. P. Rastreamento sorológico e molecular do vírus da hepatite B em homens que fazem sexo com homens em Goiânia-Goiás, utilizando o método respondent-driven sampling (RDS). 2017.136 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2017. |
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http://repositorio.bc.ufg.br/tede/handle/tede/7001 |
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por |
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por |
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Universidade Federal de Goiás |
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UFG |
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Brasil |
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Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
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Universidade Federal de Goiás |
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